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Keygen Biotech recombinant proteins mccoy
Recombinant Proteins Mccoy, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5a+medium/5a+medium/pm41196678-252-167-172
Average 86 stars, based on 1 article reviews
recombinant proteins mccoy - by Bioz Stars, 2026-11
86/100 stars

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Cell Culture:

Article Title: A combined computational and functional approach identifies FBLN5 as an amino acid metabolism-related gene and suppressing colorectal cancer progression.
Article Snippet: 0.935 million in 2020, accounting for the second-highest number of deaths due to malignant cancer in the world (Abedizadeh et al. 2024).. According to the temporal profiles and demographic predictions, the global burden of CRC will continue to grow.. The incidence of CRC is projected to increase to 3.2 million new cases and 1.6 million deaths by 2040 with most cases predicted to occur in high or very high HDI countries (EM et al. 2023).

Article Title: Artemisitene triggers calcium-dependent ferroptosis by disrupting the LSH-EWSR1 interaction in colorectal cancer
Article Snippet: .. HCT-116 and HT-29 cells were cultured in McCoy's 5A medium (KeyGEN Biotechnology, China) supplemented with 10 % fetal bovine serum (Lonsera, USA). .. HCT-116 and HT-29 cells were cultured in McCoy's 5A medium (KeyGEN Biotechnology, China) supplemented with 10 % fetal bovine serum (Lonsera, USA).

Article Title: Targeting USP28 inhibits clear cell renal cell carcinoma growth.
Article Snippet: Clear cell renal cell carcinoma (ccRCC), the most common type of kidney cancer, remains challenging to treat due to the lack of effective targeted therapies.. Although cMyc is frequently overexpressed in ccRCC, the mechanisms governing its stability are not well understood.. Here, we identify the deubiquitinating enzyme USP28 as a key regulator of c-Myc protein stability in ccRCC.

Article Title: Artemisitene triggers calcium-dependent ferroptosis by disrupting the LSH-EWSR1 interaction in colorectal cancer.
Article Snippet: .. HCT-116 and HT-29 cells were cultured in McCoy’s 5A medium (KeyGEN Biotechnology, China) supplemented with 10 % fetal bovine serum (Lonsera, USA). .. HCT-8 cells were maintained in RPMI-1640 medium (GIBCO, USA) containing 10 % FBS.

Article Title: Identification and validation of a sphingolipid metabolism-related prognostic signature for predicting prognosis and immune microenvironment-related characteristics in ovarian cancer
Article Snippet: This study utilized the human normal ovarian epithelial cell line IOSE-80 (cat. no. AW-CH0170, AnWei-sci, Shanghai, China) and the human OV cell lines SKOV3 (cat. no. SCSP-5214, National Collection ofAuthenticated Cell Cultures, Shanghai, China) and ES-2 (cat. no. SCTT1074, Wuhan Zishan Biotechnology, Wuhan, China). .. Cells were cultured in either 5A medium (cat. no. KGL1702-500, KeyGEN BioTECH, Nanjing, China) or RPMI-1640 medium (cat. no. KGL1503-500, KeyGEN BioTECH, Nanjing, China), supplemented with 10% fetal bovine serum (cat. no. A5256701, Thermo Fisher, Waltham, MA, USA) and 1% penicillin-streptomycin (cat. no. PC0315, ProteinBio, Nanjing, China). ..

Negative Control:

Article Title: IGF2BP2 promotes malignant progression of ovarian cancer by regulating protein synthesis through liquid-liquid phase separation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-IGF2BP2 Polyclonal antibody Proteintech Cat# 11601-1-AP; RRID: AB_2122672 anti-METTL3 Polyclonal antibody Proteintech Cat# 15073-1-AP; RRID: AB_2142033 anti-METTL14 Polyclonal antibody Proteintech Cat# 26158-1-AP; RRID: AB_2800447 anti-Mouse monoclonal anti-β-Actin Santa Cruz Biotechnology Cat# sc-47778; RRID: AB_626632 anti-N6-methyladenosine/m6A Rabbit mAb ABclonal Cat# A19841; RRID: AB_2862753 Goat anti-Mouse IgG-HRP Biosharp Cat# BL001A; RRID: AB_2827665 Goat anti-Rabbit IgG-HRP Biosharp Cat# BL003A; RRID: AB_2827666 anti-EDC4 Polyclonal antibody Santa Cruz Biotechnology Cat# sc-376382; RRID: AB_10988077 anti-G3BP1 Polyclonal antibody Proteintech Cat# 13057-2-AP; RRID: AB_2232034 anti-Puromycin Rabbit mAb Millipore Cat# MABE343; RRID: AB_2566826 GFP tag Polyclonal antibody Proteintech Cat# 50430-2-AP, RRID: AB_11042881 anti-c-Myc Proteintech Cat# 67447-1-Ig; RRID: AB_2882681 Anti-eIF3B Proteintech Cat# 10319-1-AP; RRID: AB_2096732 Anti-eIF3L Proteintech Cat# 11482-1-AP; RRID: AB_2877768 Anti-GCN5 Proteintech Cat# 66575-1-Ig; RRID: AB_2881935 anti-Acetylated-Lysine Cell Signaling Technology Cat #9441S; RRID: AB_331805 Negative control antibody Millipore Cat# PP64; RRID: AB_97852 Bacterial and virus strains GFP-tagged wild-type IGF2BP2 overexpression lentivirus Guangzhou FulenGen Co., Ltd. N/A GFP-tagged KH1-4 mutant IGF2BP2 overexpression lentivirus Guangzhou FulenGen Co., Ltd. N/A Chemicals, peptides, and recombinant proteins McCoy’s 5A medium KeyGEN, Nanjing, China Cat# KGL1702-500 DMEM high glucose medium KeyGEN, Nanjing, China Cat# KGL1208-500 RPMI 1640 medium KeyGEN, Nanjing, China Cat# KGL1503-500 FBS NewZerum, Christchurch, New Zealand Cat# FBS-UE500 Penicillin-streptomycin KeyGEN, Nanjing, China Cat# KGL2303-100 RIPA Servicebio, Wuhan, China Cat# G2002-30ML 1% cocktail Thermo Fisher Scientific, Waltham, MA, USA Cat# 78425 100 μg/mL cycloheximide Sigma-Aldrich, MO, USA Cat# 66-81-9 GlycoBlue Thermo Fisher Scientific, Waltham, MA, USA Cat# AM9516 HiScript ll RT SuperMix Vazyme, Nanjing, China Cat# R223-01 ChamQ Universal SYBR qPCR Master Mix Vazyme, Nanjing, China Cat# Q711-02 Triton X-100 Biofroxx, Einhausen, Germany Cat# 9002-93-1 4% paraformaldehyde Biosharp, Hefei, China Cat# BL539A Puromycin Biosharp, Hefei, China Cat# BS111-25mg Puromycin Invitrogen Cat# ant-pr-1 Crystal violet Solarbio, Beijing, China Cat# C8470 CCK8 Servicebio, Wuhan, China Cat# 4103 Matrigel Corning, NY, USA Cat# 356234 (Continued on next page) Cell Reports 44, 116511, November 25, 2025 15 ..

Virus:

Article Title: IGF2BP2 promotes malignant progression of ovarian cancer by regulating protein synthesis through liquid-liquid phase separation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-IGF2BP2 Polyclonal antibody Proteintech Cat# 11601-1-AP; RRID: AB_2122672 anti-METTL3 Polyclonal antibody Proteintech Cat# 15073-1-AP; RRID: AB_2142033 anti-METTL14 Polyclonal antibody Proteintech Cat# 26158-1-AP; RRID: AB_2800447 anti-Mouse monoclonal anti-β-Actin Santa Cruz Biotechnology Cat# sc-47778; RRID: AB_626632 anti-N6-methyladenosine/m6A Rabbit mAb ABclonal Cat# A19841; RRID: AB_2862753 Goat anti-Mouse IgG-HRP Biosharp Cat# BL001A; RRID: AB_2827665 Goat anti-Rabbit IgG-HRP Biosharp Cat# BL003A; RRID: AB_2827666 anti-EDC4 Polyclonal antibody Santa Cruz Biotechnology Cat# sc-376382; RRID: AB_10988077 anti-G3BP1 Polyclonal antibody Proteintech Cat# 13057-2-AP; RRID: AB_2232034 anti-Puromycin Rabbit mAb Millipore Cat# MABE343; RRID: AB_2566826 GFP tag Polyclonal antibody Proteintech Cat# 50430-2-AP, RRID: AB_11042881 anti-c-Myc Proteintech Cat# 67447-1-Ig; RRID: AB_2882681 Anti-eIF3B Proteintech Cat# 10319-1-AP; RRID: AB_2096732 Anti-eIF3L Proteintech Cat# 11482-1-AP; RRID: AB_2877768 Anti-GCN5 Proteintech Cat# 66575-1-Ig; RRID: AB_2881935 anti-Acetylated-Lysine Cell Signaling Technology Cat #9441S; RRID: AB_331805 Negative control antibody Millipore Cat# PP64; RRID: AB_97852 Bacterial and virus strains GFP-tagged wild-type IGF2BP2 overexpression lentivirus Guangzhou FulenGen Co., Ltd. N/A GFP-tagged KH1-4 mutant IGF2BP2 overexpression lentivirus Guangzhou FulenGen Co., Ltd. N/A Chemicals, peptides, and recombinant proteins McCoy’s 5A medium KeyGEN, Nanjing, China Cat# KGL1702-500 DMEM high glucose medium KeyGEN, Nanjing, China Cat# KGL1208-500 RPMI 1640 medium KeyGEN, Nanjing, China Cat# KGL1503-500 FBS NewZerum, Christchurch, New Zealand Cat# FBS-UE500 Penicillin-streptomycin KeyGEN, Nanjing, China Cat# KGL2303-100 RIPA Servicebio, Wuhan, China Cat# G2002-30ML 1% cocktail Thermo Fisher Scientific, Waltham, MA, USA Cat# 78425 100 μg/mL cycloheximide Sigma-Aldrich, MO, USA Cat# 66-81-9 GlycoBlue Thermo Fisher Scientific, Waltham, MA, USA Cat# AM9516 HiScript ll RT SuperMix Vazyme, Nanjing, China Cat# R223-01 ChamQ Universal SYBR qPCR Master Mix Vazyme, Nanjing, China Cat# Q711-02 Triton X-100 Biofroxx, Einhausen, Germany Cat# 9002-93-1 4% paraformaldehyde Biosharp, Hefei, China Cat# BL539A Puromycin Biosharp, Hefei, China Cat# BS111-25mg Puromycin Invitrogen Cat# ant-pr-1 Crystal violet Solarbio, Beijing, China Cat# C8470 CCK8 Servicebio, Wuhan, China Cat# 4103 Matrigel Corning, NY, USA Cat# 356234 (Continued on next page) Cell Reports 44, 116511, November 25, 2025 15 ..

Over Expression:

Article Title: IGF2BP2 promotes malignant progression of ovarian cancer by regulating protein synthesis through liquid-liquid phase separation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-IGF2BP2 Polyclonal antibody Proteintech Cat# 11601-1-AP; RRID: AB_2122672 anti-METTL3 Polyclonal antibody Proteintech Cat# 15073-1-AP; RRID: AB_2142033 anti-METTL14 Polyclonal antibody Proteintech Cat# 26158-1-AP; RRID: AB_2800447 anti-Mouse monoclonal anti-β-Actin Santa Cruz Biotechnology Cat# sc-47778; RRID: AB_626632 anti-N6-methyladenosine/m6A Rabbit mAb ABclonal Cat# A19841; RRID: AB_2862753 Goat anti-Mouse IgG-HRP Biosharp Cat# BL001A; RRID: AB_2827665 Goat anti-Rabbit IgG-HRP Biosharp Cat# BL003A; RRID: AB_2827666 anti-EDC4 Polyclonal antibody Santa Cruz Biotechnology Cat# sc-376382; RRID: AB_10988077 anti-G3BP1 Polyclonal antibody Proteintech Cat# 13057-2-AP; RRID: AB_2232034 anti-Puromycin Rabbit mAb Millipore Cat# MABE343; RRID: AB_2566826 GFP tag Polyclonal antibody Proteintech Cat# 50430-2-AP, RRID: AB_11042881 anti-c-Myc Proteintech Cat# 67447-1-Ig; RRID: AB_2882681 Anti-eIF3B Proteintech Cat# 10319-1-AP; RRID: AB_2096732 Anti-eIF3L Proteintech Cat# 11482-1-AP; RRID: AB_2877768 Anti-GCN5 Proteintech Cat# 66575-1-Ig; RRID: AB_2881935 anti-Acetylated-Lysine Cell Signaling Technology Cat #9441S; RRID: AB_331805 Negative control antibody Millipore Cat# PP64; RRID: AB_97852 Bacterial and virus strains GFP-tagged wild-type IGF2BP2 overexpression lentivirus Guangzhou FulenGen Co., Ltd. N/A GFP-tagged KH1-4 mutant IGF2BP2 overexpression lentivirus Guangzhou FulenGen Co., Ltd. N/A Chemicals, peptides, and recombinant proteins McCoy’s 5A medium KeyGEN, Nanjing, China Cat# KGL1702-500 DMEM high glucose medium KeyGEN, Nanjing, China Cat# KGL1208-500 RPMI 1640 medium KeyGEN, Nanjing, China Cat# KGL1503-500 FBS NewZerum, Christchurch, New Zealand Cat# FBS-UE500 Penicillin-streptomycin KeyGEN, Nanjing, China Cat# KGL2303-100 RIPA Servicebio, Wuhan, China Cat# G2002-30ML 1% cocktail Thermo Fisher Scientific, Waltham, MA, USA Cat# 78425 100 μg/mL cycloheximide Sigma-Aldrich, MO, USA Cat# 66-81-9 GlycoBlue Thermo Fisher Scientific, Waltham, MA, USA Cat# AM9516 HiScript ll RT SuperMix Vazyme, Nanjing, China Cat# R223-01 ChamQ Universal SYBR qPCR Master Mix Vazyme, Nanjing, China Cat# Q711-02 Triton X-100 Biofroxx, Einhausen, Germany Cat# 9002-93-1 4% paraformaldehyde Biosharp, Hefei, China Cat# BL539A Puromycin Biosharp, Hefei, China Cat# BS111-25mg Puromycin Invitrogen Cat# ant-pr-1 Crystal violet Solarbio, Beijing, China Cat# C8470 CCK8 Servicebio, Wuhan, China Cat# 4103 Matrigel Corning, NY, USA Cat# 356234 (Continued on next page) Cell Reports 44, 116511, November 25, 2025 15 ..

Mutagenesis:

Article Title: IGF2BP2 promotes malignant progression of ovarian cancer by regulating protein synthesis through liquid-liquid phase separation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-IGF2BP2 Polyclonal antibody Proteintech Cat# 11601-1-AP; RRID: AB_2122672 anti-METTL3 Polyclonal antibody Proteintech Cat# 15073-1-AP; RRID: AB_2142033 anti-METTL14 Polyclonal antibody Proteintech Cat# 26158-1-AP; RRID: AB_2800447 anti-Mouse monoclonal anti-β-Actin Santa Cruz Biotechnology Cat# sc-47778; RRID: AB_626632 anti-N6-methyladenosine/m6A Rabbit mAb ABclonal Cat# A19841; RRID: AB_2862753 Goat anti-Mouse IgG-HRP Biosharp Cat# BL001A; RRID: AB_2827665 Goat anti-Rabbit IgG-HRP Biosharp Cat# BL003A; RRID: AB_2827666 anti-EDC4 Polyclonal antibody Santa Cruz Biotechnology Cat# sc-376382; RRID: AB_10988077 anti-G3BP1 Polyclonal antibody Proteintech Cat# 13057-2-AP; RRID: AB_2232034 anti-Puromycin Rabbit mAb Millipore Cat# MABE343; RRID: AB_2566826 GFP tag Polyclonal antibody Proteintech Cat# 50430-2-AP, RRID: AB_11042881 anti-c-Myc Proteintech Cat# 67447-1-Ig; RRID: AB_2882681 Anti-eIF3B Proteintech Cat# 10319-1-AP; RRID: AB_2096732 Anti-eIF3L Proteintech Cat# 11482-1-AP; RRID: AB_2877768 Anti-GCN5 Proteintech Cat# 66575-1-Ig; RRID: AB_2881935 anti-Acetylated-Lysine Cell Signaling Technology Cat #9441S; RRID: AB_331805 Negative control antibody Millipore Cat# PP64; RRID: AB_97852 Bacterial and virus strains GFP-tagged wild-type IGF2BP2 overexpression lentivirus Guangzhou FulenGen Co., Ltd. N/A GFP-tagged KH1-4 mutant IGF2BP2 overexpression lentivirus Guangzhou FulenGen Co., Ltd. N/A Chemicals, peptides, and recombinant proteins McCoy’s 5A medium KeyGEN, Nanjing, China Cat# KGL1702-500 DMEM high glucose medium KeyGEN, Nanjing, China Cat# KGL1208-500 RPMI 1640 medium KeyGEN, Nanjing, China Cat# KGL1503-500 FBS NewZerum, Christchurch, New Zealand Cat# FBS-UE500 Penicillin-streptomycin KeyGEN, Nanjing, China Cat# KGL2303-100 RIPA Servicebio, Wuhan, China Cat# G2002-30ML 1% cocktail Thermo Fisher Scientific, Waltham, MA, USA Cat# 78425 100 μg/mL cycloheximide Sigma-Aldrich, MO, USA Cat# 66-81-9 GlycoBlue Thermo Fisher Scientific, Waltham, MA, USA Cat# AM9516 HiScript ll RT SuperMix Vazyme, Nanjing, China Cat# R223-01 ChamQ Universal SYBR qPCR Master Mix Vazyme, Nanjing, China Cat# Q711-02 Triton X-100 Biofroxx, Einhausen, Germany Cat# 9002-93-1 4% paraformaldehyde Biosharp, Hefei, China Cat# BL539A Puromycin Biosharp, Hefei, China Cat# BS111-25mg Puromycin Invitrogen Cat# ant-pr-1 Crystal violet Solarbio, Beijing, China Cat# C8470 CCK8 Servicebio, Wuhan, China Cat# 4103 Matrigel Corning, NY, USA Cat# 356234 (Continued on next page) Cell Reports 44, 116511, November 25, 2025 15 ..

Recombinant:

Article Title: IGF2BP2 promotes malignant progression of ovarian cancer by regulating protein synthesis through liquid-liquid phase separation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-IGF2BP2 Polyclonal antibody Proteintech Cat# 11601-1-AP; RRID: AB_2122672 anti-METTL3 Polyclonal antibody Proteintech Cat# 15073-1-AP; RRID: AB_2142033 anti-METTL14 Polyclonal antibody Proteintech Cat# 26158-1-AP; RRID: AB_2800447 anti-Mouse monoclonal anti-β-Actin Santa Cruz Biotechnology Cat# sc-47778; RRID: AB_626632 anti-N6-methyladenosine/m6A Rabbit mAb ABclonal Cat# A19841; RRID: AB_2862753 Goat anti-Mouse IgG-HRP Biosharp Cat# BL001A; RRID: AB_2827665 Goat anti-Rabbit IgG-HRP Biosharp Cat# BL003A; RRID: AB_2827666 anti-EDC4 Polyclonal antibody Santa Cruz Biotechnology Cat# sc-376382; RRID: AB_10988077 anti-G3BP1 Polyclonal antibody Proteintech Cat# 13057-2-AP; RRID: AB_2232034 anti-Puromycin Rabbit mAb Millipore Cat# MABE343; RRID: AB_2566826 GFP tag Polyclonal antibody Proteintech Cat# 50430-2-AP, RRID: AB_11042881 anti-c-Myc Proteintech Cat# 67447-1-Ig; RRID: AB_2882681 Anti-eIF3B Proteintech Cat# 10319-1-AP; RRID: AB_2096732 Anti-eIF3L Proteintech Cat# 11482-1-AP; RRID: AB_2877768 Anti-GCN5 Proteintech Cat# 66575-1-Ig; RRID: AB_2881935 anti-Acetylated-Lysine Cell Signaling Technology Cat #9441S; RRID: AB_331805 Negative control antibody Millipore Cat# PP64; RRID: AB_97852 Bacterial and virus strains GFP-tagged wild-type IGF2BP2 overexpression lentivirus Guangzhou FulenGen Co., Ltd. N/A GFP-tagged KH1-4 mutant IGF2BP2 overexpression lentivirus Guangzhou FulenGen Co., Ltd. N/A Chemicals, peptides, and recombinant proteins McCoy’s 5A medium KeyGEN, Nanjing, China Cat# KGL1702-500 DMEM high glucose medium KeyGEN, Nanjing, China Cat# KGL1208-500 RPMI 1640 medium KeyGEN, Nanjing, China Cat# KGL1503-500 FBS NewZerum, Christchurch, New Zealand Cat# FBS-UE500 Penicillin-streptomycin KeyGEN, Nanjing, China Cat# KGL2303-100 RIPA Servicebio, Wuhan, China Cat# G2002-30ML 1% cocktail Thermo Fisher Scientific, Waltham, MA, USA Cat# 78425 100 μg/mL cycloheximide Sigma-Aldrich, MO, USA Cat# 66-81-9 GlycoBlue Thermo Fisher Scientific, Waltham, MA, USA Cat# AM9516 HiScript ll RT SuperMix Vazyme, Nanjing, China Cat# R223-01 ChamQ Universal SYBR qPCR Master Mix Vazyme, Nanjing, China Cat# Q711-02 Triton X-100 Biofroxx, Einhausen, Germany Cat# 9002-93-1 4% paraformaldehyde Biosharp, Hefei, China Cat# BL539A Puromycin Biosharp, Hefei, China Cat# BS111-25mg Puromycin Invitrogen Cat# ant-pr-1 Crystal violet Solarbio, Beijing, China Cat# C8470 CCK8 Servicebio, Wuhan, China Cat# 4103 Matrigel Corning, NY, USA Cat# 356234 (Continued on next page) Cell Reports 44, 116511, November 25, 2025 15 ..

Real-time Polymerase Chain Reaction:

Article Title: IGF2BP2 promotes malignant progression of ovarian cancer by regulating protein synthesis through liquid-liquid phase separation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-IGF2BP2 Polyclonal antibody Proteintech Cat# 11601-1-AP; RRID: AB_2122672 anti-METTL3 Polyclonal antibody Proteintech Cat# 15073-1-AP; RRID: AB_2142033 anti-METTL14 Polyclonal antibody Proteintech Cat# 26158-1-AP; RRID: AB_2800447 anti-Mouse monoclonal anti-β-Actin Santa Cruz Biotechnology Cat# sc-47778; RRID: AB_626632 anti-N6-methyladenosine/m6A Rabbit mAb ABclonal Cat# A19841; RRID: AB_2862753 Goat anti-Mouse IgG-HRP Biosharp Cat# BL001A; RRID: AB_2827665 Goat anti-Rabbit IgG-HRP Biosharp Cat# BL003A; RRID: AB_2827666 anti-EDC4 Polyclonal antibody Santa Cruz Biotechnology Cat# sc-376382; RRID: AB_10988077 anti-G3BP1 Polyclonal antibody Proteintech Cat# 13057-2-AP; RRID: AB_2232034 anti-Puromycin Rabbit mAb Millipore Cat# MABE343; RRID: AB_2566826 GFP tag Polyclonal antibody Proteintech Cat# 50430-2-AP, RRID: AB_11042881 anti-c-Myc Proteintech Cat# 67447-1-Ig; RRID: AB_2882681 Anti-eIF3B Proteintech Cat# 10319-1-AP; RRID: AB_2096732 Anti-eIF3L Proteintech Cat# 11482-1-AP; RRID: AB_2877768 Anti-GCN5 Proteintech Cat# 66575-1-Ig; RRID: AB_2881935 anti-Acetylated-Lysine Cell Signaling Technology Cat #9441S; RRID: AB_331805 Negative control antibody Millipore Cat# PP64; RRID: AB_97852 Bacterial and virus strains GFP-tagged wild-type IGF2BP2 overexpression lentivirus Guangzhou FulenGen Co., Ltd. N/A GFP-tagged KH1-4 mutant IGF2BP2 overexpression lentivirus Guangzhou FulenGen Co., Ltd. N/A Chemicals, peptides, and recombinant proteins McCoy’s 5A medium KeyGEN, Nanjing, China Cat# KGL1702-500 DMEM high glucose medium KeyGEN, Nanjing, China Cat# KGL1208-500 RPMI 1640 medium KeyGEN, Nanjing, China Cat# KGL1503-500 FBS NewZerum, Christchurch, New Zealand Cat# FBS-UE500 Penicillin-streptomycin KeyGEN, Nanjing, China Cat# KGL2303-100 RIPA Servicebio, Wuhan, China Cat# G2002-30ML 1% cocktail Thermo Fisher Scientific, Waltham, MA, USA Cat# 78425 100 μg/mL cycloheximide Sigma-Aldrich, MO, USA Cat# 66-81-9 GlycoBlue Thermo Fisher Scientific, Waltham, MA, USA Cat# AM9516 HiScript ll RT SuperMix Vazyme, Nanjing, China Cat# R223-01 ChamQ Universal SYBR qPCR Master Mix Vazyme, Nanjing, China Cat# Q711-02 Triton X-100 Biofroxx, Einhausen, Germany Cat# 9002-93-1 4% paraformaldehyde Biosharp, Hefei, China Cat# BL539A Puromycin Biosharp, Hefei, China Cat# BS111-25mg Puromycin Invitrogen Cat# ant-pr-1 Crystal violet Solarbio, Beijing, China Cat# C8470 CCK8 Servicebio, Wuhan, China Cat# 4103 Matrigel Corning, NY, USA Cat# 356234 (Continued on next page) Cell Reports 44, 116511, November 25, 2025 15 ..



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Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon (HT29) and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.

Journal: Advances in Radiation Oncology

Article Title: Synergistic Effect of Linebacker-1 With Radiation Therapy in a Mouse Lung Cancer Model

doi: 10.1016/j.adro.2026.102051

Figure Lengend Snippet: Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon (HT29) and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.

Article Snippet: A549 cells were cultured in RPMI cell culture media (Gibco), the HT29 cell line was cultured in McCoy’s 5A medium (ATCC), and the LLC1 cell was cultured in Dulbecco’s Modified Eagle Media (Gibco).

Techniques: In Vitro, Growth Assay, Clonogenic Cell Survival Assay, Generated