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5a medium  (Danaher Inc)


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    Danaher Inc 5a medium
    5a Medium, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5a+medium/HyClone+McCoy's+5A+powder+medium/pm35115657-490-2-7
    Average 92 stars, based on 4 article reviews
    5a medium - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Loss of EMI1 compromises chromosome stability and is associated with cellular transformation in colonic epithelial cell contexts.
    Article Snippet: .. HCT116 cells were cultured in McCoy’s 5A medium (Cytiva HyClone, Vancouver, BC, Canada) supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA), while SW48 were cultured in Leibovitz’s L-15 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Cytiva HyClone). .. 1CT and A1309 were cultured in Dulbecco’s Modified Eagle Medium with High Glucose/Medium 199 (Cytiva HyClone) and supplemented with 10% cosmic calf serum (Cytiva HyClone).

    Article Title: Reduced SKP2 Expression Adversely Impacts Genome Stability and Promotes Cellular Transformation in Colonic Epithelial Cells
    Article Snippet: .. HCT116 cells were cultured in modified McCoy’s 5A medium (Cytiva HyClone, Vancouver, BC, Canada) supplemented with 10% fetal bovine serum (Sigma-Aldrich, Oakville, ON, Canada), while 1CT and A1309 cells were cultured in Dulbecco’s Modified Eagle Medium with high glucose/medium 199 (Cytiva HyClone, Vancouver, BC, Canada) supplemented with 2% cosmic calf serum (Cytiva HyClone, Vancouver, BC, Canada). ..

    Article Title: Anterior gradient 2 regulates cancer progression in TP53 ‑wild‑type esophageal squamous cell carcinoma.
    Article Snippet: Anterior gradient 2 (AGR2) reportedly promotes tumor growth and has an unfavorable impact on survival in several cancers.. However, no comprehensive functional anal‐ ysis of AGR2 in esophageal squamous cell carcinoma (ESCC) has been performed.. In the present study, the function and clinical significance of AGR2 were examined using ESCC cell lines and clinical samples.

    Modification:

    Article Title: Reduced SKP2 Expression Adversely Impacts Genome Stability and Promotes Cellular Transformation in Colonic Epithelial Cells
    Article Snippet: .. HCT116 cells were cultured in modified McCoy’s 5A medium (Cytiva HyClone, Vancouver, BC, Canada) supplemented with 10% fetal bovine serum (Sigma-Aldrich, Oakville, ON, Canada), while 1CT and A1309 cells were cultured in Dulbecco’s Modified Eagle Medium with high glucose/medium 199 (Cytiva HyClone, Vancouver, BC, Canada) supplemented with 2% cosmic calf serum (Cytiva HyClone, Vancouver, BC, Canada). ..



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    Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon <t>(HT29)</t> and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.
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    Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon (HT29) and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.

    Journal: Advances in Radiation Oncology

    Article Title: Synergistic Effect of Linebacker-1 With Radiation Therapy in a Mouse Lung Cancer Model

    doi: 10.1016/j.adro.2026.102051

    Figure Lengend Snippet: Biochemical structures of Linebacker-1 (LB1) and myricetin and antineoplastic effect of LB1 with/without radiation in human colon and lung cell lines. (A) Myricetin (left) and LB1 (right) showing with a red circle for the OH ion’s replacement by a cloride ion (Cl). (B) Schematic diagram of the treatment design using LB1 for in vitro study. (C) and (D) MTT growth assay using OD value 570 nm, showing the antitumor effect of LB1 in titrated doses for both human colon (HT29) and lung (A549) cancer cell lines, respectively. (E) Bar graph representing the colony count numbers from the clonogenic survival assay after LB1 treatment. (F) Schematic diagram showing the treatment design for in vitro LB1 and radiation treatment. Doses of 0, 25, 50, and 75 µM LB1, along with single radiation fractions of 0, 2, 4, and 6 Gy, were used in the clonogenic survival study. (G) For the clonogenic survival assay, colony counts are shown for A549 cells treated with 0, 2, 4, and 6 Gy of radiation, with or without LB1, at 0, 25, 50, and 75 µM. (H) colony count for A549 and Lewis lung carcinoma (LLC1) cell line , respectively. Graphs are generated from the average of at least 3 study repeats. The SD of the multiple independent repeats is shown, while statistical significance is shown by *** P < .001. Abbreviation : OD = optical density; OH = hydroxide ion; MTT = methyl dimethylthiazol diphenyltetrazolium bromide; RT = radiation therapy.

    Article Snippet: A549 cells were cultured in RPMI cell culture media (Gibco), the HT29 cell line was cultured in McCoy’s 5A medium (ATCC), and the LLC1 cell was cultured in Dulbecco’s Modified Eagle Media (Gibco).

    Techniques: In Vitro, Growth Assay, Clonogenic Cell Survival Assay, Generated