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5 × molony murine leukemia virus reverse transcriptase (m-mlv rt) buffer  (Promega)

 
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    Promega 5 × molony murine leukemia virus reverse transcriptase (m-mlv rt) buffer
    5 × Molony Murine Leukemia Virus Reverse Transcriptase (M Mlv Rt) Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5%C3%97+reverse-transcriptase+buffer/5+%C3%97+molony+murine+leukemia+virus+reverse+transcriptase++m+mlv+rt++buffer/pmc04751446-108-39-68
    Average 90 stars, based on 1 article reviews
    5 × molony murine leukemia virus reverse transcriptase (m-mlv rt) buffer - by Bioz Stars, 2026-11
    90/100 stars

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    Reverse Transcription:

    Article Title: Antioxidant Activity and Anti-wrinkle Effects of Aceriphyllum rossii Leaf Ethanol Extract
    Article Snippet: .. The total RNA from the human dermal fibroblasts was extracted with 1 mL of TRIzol (Invitrogen, CA) per the manufacturer instructions, and 5 μg samples were reverse-transcribed in 40-μL of a reaction mixture containing 8 μL of 5 × Molony murine leukemia virus reverse transcriptase (M-MLV RT) buffer, 3 μL of dNTPs (10 mM), 0.45 μL of RNasein (40 U/μL), 0.3 μL of M-MLV reverse transcriptase (200 U/μL; Promega, USA), and 1.5 μL of oligo dT (50 pM/μL; Bioneer, Korea). .. Single-stranded cDNA was amplified by PCR using 4 μL of 5 × green Go Taq Flexi buffer, 0.4 μL of dNTPs (10 mM), 0.1 μL Taq polymerase (5 U/μL), 1.2 μL of MgCl 2 (25 mM; Promega, USA), and 0.4 μL each of (20 pM/μL) specific sense and anti-sense primers for MMP-1 or β-actin.

    Virus:

    Article Title: Antioxidant Activity and Anti-wrinkle Effects of Aceriphyllum rossii Leaf Ethanol Extract
    Article Snippet: .. The total RNA from the human dermal fibroblasts was extracted with 1 mL of TRIzol (Invitrogen, CA) per the manufacturer instructions, and 5 μg samples were reverse-transcribed in 40-μL of a reaction mixture containing 8 μL of 5 × Molony murine leukemia virus reverse transcriptase (M-MLV RT) buffer, 3 μL of dNTPs (10 mM), 0.45 μL of RNasein (40 U/μL), 0.3 μL of M-MLV reverse transcriptase (200 U/μL; Promega, USA), and 1.5 μL of oligo dT (50 pM/μL; Bioneer, Korea). .. Single-stranded cDNA was amplified by PCR using 4 μL of 5 × green Go Taq Flexi buffer, 0.4 μL of dNTPs (10 mM), 0.1 μL Taq polymerase (5 U/μL), 1.2 μL of MgCl 2 (25 mM; Promega, USA), and 0.4 μL each of (20 pM/μL) specific sense and anti-sense primers for MMP-1 or β-actin.



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    Journal: iScience

    Article Title: Time course transcriptomic profiling suggests Crp/Fnr transcriptional regulation of nosZ gene in a N 2 O-reducing thermophile

    doi: 10.1016/j.isci.2024.111074

    Figure Lengend Snippet:

    Article Snippet: Maxima H Minus Reverse Transcriptase (200U/μL) with 5×RT Buffer , ThermoFisher , Cat# EP0751.

    Techniques: Virus, Recombinant, Reverse Transcription, Isolation, Control, Sequencing, Mass Spectrometry, Software, In Silico, Targeted Proteomics