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Pyrosequencing Inc next-generation sequencing 454-pyrosequencing
Next Generation Sequencing 454 Pyrosequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/454-generated+pyrosequencing/next+generation+sequencing+454/pmc06367363-26-1-4
Average 90 stars, based on 1 article reviews
next-generation sequencing 454-pyrosequencing - by Bioz Stars, 2026-11
90/100 stars

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Next-Generation Sequencing:

Article Title: Molecular and morphological sex differentiation in sablefish (Anoplopoma fimbria), a marine teleost with XX/XY sex determination.
Article Snippet: Phenotypic sex of an organism is determined by molecular changes in the gonads, so-called molecular sex differentiation, which should precede the rise of cellular or anatomical sex-distinguishing features.. This study characterized molecular and morphological sex differentiation in sablefish (Anoplopoma fimbria), a marine teleost with established XX/XY genotypic sex determination.. Next generation sequencing was conducted on sablefish ovarian and testicular mRNAs to obtain sequences for transcripts associated with vertebrate sex determination and differentiation and early reproductive development.

Article Title: The use of -omics tools for assessing biodeterioration of cultural heritage: A review
Article Snippet: A holistic perspective on the universe of living microorganisms, their metabolism and environment, is called systems biology.. This framework is a compelling way to diagnose the biodeterioration of cultural heritage (CH).. It is important to understand both the types of microorganisms present on objects, their metabolic functions, and the impact of the environment on microorganisms.

Isolation:

Article Title: Molecular and morphological sex differentiation in sablefish (Anoplopoma fimbria), a marine teleost with XX/XY sex determination.
Article Snippet: Phenotypic sex of an organism is determined by molecular changes in the gonads, so-called molecular sex differentiation, which should precede the rise of cellular or anatomical sex-distinguishing features.. This study characterized molecular and morphological sex differentiation in sablefish (Anoplopoma fimbria), a marine teleost with established XX/XY genotypic sex determination.. Next generation sequencing was conducted on sablefish ovarian and testicular mRNAs to obtain sequences for transcripts associated with vertebrate sex determination and differentiation and early reproductive development.

Western Blot:

Article Title: Comparison of Metatranscriptomic Samples Based on k- Tuple Frequencies
Article Snippet: .. We also analyze eight metagenomic and eight metatranscriptomic samples from the Western English Channel sequenced with pyrosequencing 454 platform (Dataset11 in ). ..

Purification:

Article Title: The salmon louse genome may be much larger than sequencing suggests
Article Snippet: .. The fact that the six independent genome assemblies are congruent in content despite originating from DNA purified from multiple origins of L. salmonis in different laboratories and sequenced using various sequencing platforms (Illumina, 454 pyrosequencing, Oxford nanopore, PacBio and Sanger) may suggest that no parts of the genome are systematically missed by the purification and sequencing protocols applied. ..

Sequencing:

Article Title: The salmon louse genome may be much larger than sequencing suggests
Article Snippet: .. The fact that the six independent genome assemblies are congruent in content despite originating from DNA purified from multiple origins of L. salmonis in different laboratories and sequenced using various sequencing platforms (Illumina, 454 pyrosequencing, Oxford nanopore, PacBio and Sanger) may suggest that no parts of the genome are systematically missed by the purification and sequencing protocols applied. ..

Article Title: The use of -omics tools for assessing biodeterioration of cultural heritage: A review
Article Snippet: A holistic perspective on the universe of living microorganisms, their metabolism and environment, is called systems biology.. This framework is a compelling way to diagnose the biodeterioration of cultural heritage (CH).. It is important to understand both the types of microorganisms present on objects, their metabolic functions, and the impact of the environment on microorganisms.

Mass Spectrometry:

Article Title: The use of -omics tools for assessing biodeterioration of cultural heritage: A review
Article Snippet: A holistic perspective on the universe of living microorganisms, their metabolism and environment, is called systems biology.. This framework is a compelling way to diagnose the biodeterioration of cultural heritage (CH).. It is important to understand both the types of microorganisms present on objects, their metabolic functions, and the impact of the environment on microorganisms.

Liquid Chromatography:

Article Title: The use of -omics tools for assessing biodeterioration of cultural heritage: A review
Article Snippet: A holistic perspective on the universe of living microorganisms, their metabolism and environment, is called systems biology.. This framework is a compelling way to diagnose the biodeterioration of cultural heritage (CH).. It is important to understand both the types of microorganisms present on objects, their metabolic functions, and the impact of the environment on microorganisms.

Liquid Chromatography with Mass Spectroscopy:

Article Title: The use of -omics tools for assessing biodeterioration of cultural heritage: A review
Article Snippet: A holistic perspective on the universe of living microorganisms, their metabolism and environment, is called systems biology.. This framework is a compelling way to diagnose the biodeterioration of cultural heritage (CH).. It is important to understand both the types of microorganisms present on objects, their metabolic functions, and the impact of the environment on microorganisms.

other:

Article Title: In vitro model systems for exploring oral biofilms: From single-species populations to complex multi-species communities
Article Snippet: TABLE 3 Sequencing platform (Reference) Sequencing chemistry Read length Sequencing depth Consensus accuracy 454 GS FLX+ a ( Kistler et al., 2015 ; Koopman et al., 2015 ; Nance et al., 2013 ) Pyrosequencing Up to 1000 bp 700 Mb 99.997 Illumina MiSeq ( Agnello et al., 2017 ; Koopman et al., 2016 ) Sequencing by synthesis 2 × 150 2 × 250 2 × 300 4.5–5.1 Gb 7.5–8.5 Gb 13.2–15 Gb 80% bases >99.9 75% bases >99.9 70% bases >99.9 Illumina HiSeq ( Edlund et al., 2013 ) Sequencing by synthesis 2 × 125 450–500 Gb 80% bases >99.9 IonTorrent PGM ( Fernandez et al., 2017 ) Ion semiconductor Up to 400 bp Up to 2 Gb >99.0 Open in a separate window a Technology is no longer supported by manufacture.

Article Title: Diversity and Novelty of Venom Peptides in Vermivorous Cone Snails, Subgenus Rhizoconus (Gastropoda: Mollusca)
Article Snippet: The conopeptide sequences identified in this study in the C. miles transcriptome significantly expanded the known diversity of conopeptides in this species based on a previous analysis of transcriptome (cDNA sequencing using 454 pyrosequencing) and proteome data obtained from a C. miles specimen collected in the Great Barrier Reef, Australia [15], as well as the five O1-sequences obtained from cDNA cloning and sequencing from C. miles specimens collected from Hainan Province [31].

Article Title: Soil Fungal Community Structure in Boreal Pine Forests: From Southern to Subarctic Areas of Finland
Article Snippet: The methods used in each study for DNA extraction, primer pairs (gITS7 and ITS4) and high-throughput sequencing (454 pyrosequencing) were identical, and have been described in detail by , .



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The 16S rRNA sequence (V1 to V5)-based taxonomic tree corresponding to NCBI taxonomy database in clinically healthy birds representing studied farms. The size/radius of the node corresponds to the number of hits for each taxonomic node. Black color of radius corresponds to CHS; FRS as green, and OHS as blue. Full color version available online.

Journal: Poultry Science

Article Title: Microbial communities present in the lower respiratory tract of clinically healthy birds in Pakistan

doi: 10.3382/ps/pev010

Figure Lengend Snippet: The 16S rRNA sequence (V1 to V5)-based taxonomic tree corresponding to NCBI taxonomy database in clinically healthy birds representing studied farms. The size/radius of the node corresponds to the number of hits for each taxonomic node. Black color of radius corresponds to CHS; FRS as green, and OHS as blue. Full color version available online.

Article Snippet: We sampled the lower respiratory microbiome of each bird via tracheo-bronchoalveolar lavage ( T-BAL ) and subsequently sequenced 16S rRNA gene by next generating sequencing ( NGS ) (454-pyrosequencing); the region is considered highly conserved across different species of bacteria and archaea (Clarridge, 2004 ; Streit and Schmitz, 2004 ; Schuster, 2008 ).

Techniques: Sequencing