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Solarbio Inc 2×sds-page loading buffer

2×Sds Page Loading Buffer, supplied by Solarbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4+%C3%97+sds+sample+loading+buffer/4%C3%97+sds+page+loading+buffer/pmc09057979-34-0-4
Average 90 stars, based on 1 article reviews
2×sds-page loading buffer - by Bioz Stars, 2026-11
90/100 stars

Images

1) Product Images from "An antibody-based proximity labeling protocol to identify biotinylated interactors of SARS-CoV-2"

Article Title: An antibody-based proximity labeling protocol to identify biotinylated interactors of SARS-CoV-2

Journal: STAR Protocols

doi: 10.1016/j.xpro.2022.101406


Figure Legend Snippet:

Techniques Used: Recombinant, Protease Inhibitor, Clone Assay, Bicinchoninic Acid Protein Assay, Labeling, Software

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other:

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Article Snippet: Tri(1,3-dichloro-2-propyl)phosphate (TDCPP) is one of the most widely used organophosphorus flame retardants in consumer products.. TDCPP has been confirmed to be neurotoxic, but its mechanism has not been clarified and may be related to mitophagy.. AMBRA1 can promote neurological autophagy, but whether AMBRA1 is involved in the mechanism of TDCPPinduced neurotoxicity has not been elucidated.

Article Title: An antibody-based proximity labeling protocol to identify biotinylated interactors of SARS-CoV-2
Article Snippet: 2×SDS-PAGE loading buffer , Solarbio , Cat# P1018.

Article Title: A Drug-Elicitable Alternative-Splicing Module (DreAM) for Tunable AAV Expression and Controlled Myocardial Regeneration
Article Snippet: After centrifugation, supernatant was diluted in 4× SDS-PAGE loading buffer (B1016, Solarbio).

Article Title: Cerebral protection of epigallocatechin gallate (EGCG) via preservation of mitochondrial function and ERK inhibition in a rat resuscitation model
Article Snippet: After centrifugation, total protein concentration was determined using a BCA Protein Assay Reagent Kit (P0010; Beyotime Biotechnology, USA) with the proteins extracted from the supernatant, which were then denatured at 100°C for 15 mins in 4× SDS-PAGE loading buffer (P1015; Solarbio).

Bradford Protein Assay:

Article Title: Substance P containing peptide gene delivery vectors for specifically transfecting glioma cells mediated by a neurokinin-1 receptor.
Article Snippet: Gene therapy provides a promising treatment for glioblastoma multiforme, which mainly depends on two key aspects, crossing the blood brain barrier (BBB) effectively and transfecting target cells selectively.. In this work, we reported a series of peptide-based vectors for transfecting glioma cells specifically consisting of several functional segments including a cell-penetrating peptide, targeting segment substance P (SP), an endosomal escape segment, a PEG linker and a stearyl moiety.. The conformations and DNA-loading capacities of peptide vectors and the self-assembly behaviors of peptide/pGL3 complexes were characterized.

Incubation:

Article Title: Discovery of dual inhibitors of topoisomerase I and Cyclooxygenase-2 for colon cancer therapy.
Article Snippet: Novel tolfenamic acid derivatives based on the structure of I-1 were designed and synthesized to improve its poor target inhibition and solubility.. Among them, W10 was identified as a potent dual-target inhibitor of Topo I (IC50 = 0.90 ± 0.17 μM) and COX-2 (IC50 = 2.31 ± 0.07 μM) with improved water solubility (32.33 μg/mL).. Moreover, W10 also exhibited fairly potent anti-proliferative and pro-apoptosis activity via the mitochondrial pathway, as well as suppressed aberrant NF-κB/IκB activation in colon cancer cells in vitro.

Protease Inhibitor:

Article Title: Discovery of dual inhibitors of topoisomerase I and Cyclooxygenase-2 for colon cancer therapy.
Article Snippet: Novel tolfenamic acid derivatives based on the structure of I-1 were designed and synthesized to improve its poor target inhibition and solubility.. Among them, W10 was identified as a potent dual-target inhibitor of Topo I (IC50 = 0.90 ± 0.17 μM) and COX-2 (IC50 = 2.31 ± 0.07 μM) with improved water solubility (32.33 μg/mL).. Moreover, W10 also exhibited fairly potent anti-proliferative and pro-apoptosis activity via the mitochondrial pathway, as well as suppressed aberrant NF-κB/IκB activation in colon cancer cells in vitro.

Bicinchoninic Acid Protein Assay:

Article Title: Discovery of dual inhibitors of topoisomerase I and Cyclooxygenase-2 for colon cancer therapy.
Article Snippet: Novel tolfenamic acid derivatives based on the structure of I-1 were designed and synthesized to improve its poor target inhibition and solubility.. Among them, W10 was identified as a potent dual-target inhibitor of Topo I (IC50 = 0.90 ± 0.17 μM) and COX-2 (IC50 = 2.31 ± 0.07 μM) with improved water solubility (32.33 μg/mL).. Moreover, W10 also exhibited fairly potent anti-proliferative and pro-apoptosis activity via the mitochondrial pathway, as well as suppressed aberrant NF-κB/IκB activation in colon cancer cells in vitro.

SDS Page:

Article Title: TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus
Article Snippet: .. The final pellet was dissolved in 4 × SDS loading buffer (P1016; solarbio) for SDS-PAGE and Western blotting. ..

Western Blot:

Article Title: TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus
Article Snippet: .. The final pellet was dissolved in 4 × SDS loading buffer (P1016; solarbio) for SDS-PAGE and Western blotting. ..

Staining:

Article Title: Effective Antibacterial Activity of Degradable Copper-Doped Phosphate-Based Glass Nanozymes.
Article Snippet: Copper-containing antimicrobials are highly valuable in the field of medical disinfectants owing to their well-known high antimicrobial efficacy.. Artificially synthesized nanozymes which can increase the level of reactive oxygen species (ROS) in the bacterial system have become research hotspots.. Herein, we describe the design and fabrication of degradable Cu-doped phosphatebased glass (Cu-PBG) nanozyme, which can achieve excellent antibacterial effects against Gram-positive and Gram-negative bacteria.



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Image Search Results


Journal: Investigative Ophthalmology & Visual Science

Article Title: Loss of Cathepsin K Impairs Collagen Biogenesis and Enhances Actin Polymerization in Trabecular Meshwork

doi: 10.1167/iovs.67.1.63

Figure Lengend Snippet: Materials and Reagents Used

Article Snippet: 4× SDS sample loading buffer , J63615 , Thermo Fisher Scientific.

Techniques: Knockdown, Control, Membrane, Bicinchoninic Acid Protein Assay, Pore Size, Staining, Western Blot, Recombinant, Mass Spectrometry, Colorimetric Assay, In Vivo