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Nippon Gene Co Ltd 3x loading buffer orange g
3x Loading Buffer Orange G, supplied by Nippon Gene Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3x+loading+buffer/6+buffer+g+loading+orange+x/pm40708054-105-5-10
Average 86 stars, based on 1 article reviews
3x loading buffer orange g - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Staining:

Article Title: The effect of guide RNA thermal denaturation on the quality of Cas9 ribonucleoprotein-loaded lipid nanoparticle formulations
Article Snippet: Native-PAGE for sgRNAs was performed using a MiniPROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad Laboratories, Inc., San Francisco, USA). .. Then, 100 ng of sgRNA was added with a 6× Loading of Buffer Orange G (Nippon Gene Co., Ltd, Tokyo, Japan) and was then run in MiniPROTEAN TGX Gels (4–15%) (Bio-Rad Laboratories) in Tris/Glycine buffer (2.5 mM Tris, 19.2 mM Glycine (pH 8.3) (Bio-Rad Laboratories)) at 100 V for 100 min. Gels were stained with SYBR Green II Nucleic Acid Gel Stain (TaKaRa Bio Inc., Shiga, Japan) and visualized with a Printgraph CMOS I (ATTO Corporation, Tokyo, Japan). .. Native-PAGE for RNPs was performed using an M Rapidus Mini-Slab Electrophoresis Tank (ATTO Corporation).

Article Title: Micro-scale genetic structure and genetic variation of Neotricula aperta (Gastropoda: Pomatiopsidae), the intermediate host of Schistosoma mekongi (Digenea: Schistosomatidae) in Champasak Province, Laos.
Article Snippet: .. PCR products were stained with 3X Loading Buffer Orange G (Nippon Gene Co. Ltd., Japan) and examined by electrophoresis on a 2% agarose gel. .. Successfully amplified products were purified using the FastGeneTM Gel/PCR Extraction Kit (Nippon Genetics Co. Ltd., Japan), following the manufacturer’s instructions.

SYBR Green Assay:

Article Title: The effect of guide RNA thermal denaturation on the quality of Cas9 ribonucleoprotein-loaded lipid nanoparticle formulations
Article Snippet: Native-PAGE for sgRNAs was performed using a MiniPROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad Laboratories, Inc., San Francisco, USA). .. Then, 100 ng of sgRNA was added with a 6× Loading of Buffer Orange G (Nippon Gene Co., Ltd, Tokyo, Japan) and was then run in MiniPROTEAN TGX Gels (4–15%) (Bio-Rad Laboratories) in Tris/Glycine buffer (2.5 mM Tris, 19.2 mM Glycine (pH 8.3) (Bio-Rad Laboratories)) at 100 V for 100 min. Gels were stained with SYBR Green II Nucleic Acid Gel Stain (TaKaRa Bio Inc., Shiga, Japan) and visualized with a Printgraph CMOS I (ATTO Corporation, Tokyo, Japan). .. Native-PAGE for RNPs was performed using an M Rapidus Mini-Slab Electrophoresis Tank (ATTO Corporation).

other:

Article Title: Protocol for assembling photostable fluorescence-labeled tiny DNA origami blocks to observe kinesin cooperation
Article Snippet: 6 x loading buffer Orange G , NIPPON GENE CO., LTD. , Cat# 317-90251.

Polymerase Chain Reaction:

Article Title: Micro-scale genetic structure and genetic variation of Neotricula aperta (Gastropoda: Pomatiopsidae), the intermediate host of Schistosoma mekongi (Digenea: Schistosomatidae) in Champasak Province, Laos.
Article Snippet: .. PCR products were stained with 3X Loading Buffer Orange G (Nippon Gene Co. Ltd., Japan) and examined by electrophoresis on a 2% agarose gel. .. Successfully amplified products were purified using the FastGeneTM Gel/PCR Extraction Kit (Nippon Genetics Co. Ltd., Japan), following the manufacturer’s instructions.

Electrophoresis:

Article Title: Micro-scale genetic structure and genetic variation of Neotricula aperta (Gastropoda: Pomatiopsidae), the intermediate host of Schistosoma mekongi (Digenea: Schistosomatidae) in Champasak Province, Laos.
Article Snippet: .. PCR products were stained with 3X Loading Buffer Orange G (Nippon Gene Co. Ltd., Japan) and examined by electrophoresis on a 2% agarose gel. .. Successfully amplified products were purified using the FastGeneTM Gel/PCR Extraction Kit (Nippon Genetics Co. Ltd., Japan), following the manufacturer’s instructions.

Agarose Gel Electrophoresis:

Article Title: Micro-scale genetic structure and genetic variation of Neotricula aperta (Gastropoda: Pomatiopsidae), the intermediate host of Schistosoma mekongi (Digenea: Schistosomatidae) in Champasak Province, Laos.
Article Snippet: .. PCR products were stained with 3X Loading Buffer Orange G (Nippon Gene Co. Ltd., Japan) and examined by electrophoresis on a 2% agarose gel. .. Successfully amplified products were purified using the FastGeneTM Gel/PCR Extraction Kit (Nippon Genetics Co. Ltd., Japan), following the manufacturer’s instructions.



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