Software:Article Title: GABAergic Progenitor Cell Graft Rescues Cognitive Deficits in Fragile X Syndrome Mice.
Article Snippet: ACG = ACGbase + ACGburst (3) Spike Sorting: The raw signals were imported into LabChart (A-M Systems, Sequim, WA) for high-pass filtering, and the signals with frequencies below 250 Hz were analyzed. .. Subsequently, threshold detection combined with principal component analysis from SpikeSort 3D software (NeuraLynx, Bozeman, MT) was employed to automatically transfer spike signals to 2D partitions. ..
Article Title: The interpeduncular nucleus blunts the rewarding effect of nicotine.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SpikeSort3D software Neuralynx https://neuralynx.fh-co.com/ research-software/spikesort-3d/ Affinity Designer Serif RRID:SCR_016952 ezTrack Pennington et al.64 RRID:SCR_021496 FIJI Schindelin et al.65 RRID:SCR_002285 Other One-axis Oil Hydraulic Micromanipulator Narishige Cat#MO-10 PC-10 puller Narishige RRID:SCR_022057 Capillary micropipette for PCR 10 ml Drummond Cat#5-000-1001-X10 P-87 Flaming/Brown Micropipette Puller Sutter Instruments Cat#P87 Thin-wall borosilicate glass Warner Instruments Cat#G150TF-3 Super-Bond Universal Kit Sun Medical Cat#K058E Y-maze apparatus Imetronic https://www.imetronic.com/devices/y-maze/ Axoclamp 200B Microelectrode Amplifier Molecular Devices Cat#AxOPATCH 200B-2 Digidata 1550A Low-Noise Data Acquisition Digitizer Molecular Devices Cat#5035919A GeneClamp 500 amplifier Axon Instruments N/A 8-way, 12-way, and 2-way 4-port electric rotary valve Bio-Chem Fluidics https://www.arcmedgroup.com/pumps- valves#electric-rotary-valves SliceScope upright microscope Scientifica Cat#S-Scope-II ROLERA Bolt Scientific CMOS Camera QImaging Cat#01-ROL-BOLT-M-12 Halogen lamp unit Olympus Cat#TH4-200 Compresstome VF-200 Precisionary Instruments Cat#VF-200 uPump Microinjector with Internal Pressure Source World Precision Instruments Cat#SYS-PV820 Dual Small Animal Stereotaxic w/ Digital Display David Kopf Instruments Cat#942-WA Thomas Tetrode Mini Matrix microdrive system Thomas Recording Cat#AN000423 Tetrode for Tetrode Mini Matrix (Tip A) Thomas Recording Cat#AN000259 Quartzglass microinjection pipettes for Thomas microdrive Thomas Recording Cat#AN000155 Digital Lynx 4SX Neuralynx Cat#31-0605-0141, https://neuralynx.fh-co.com/researchhardware/data-acquisition/digital-lynx-sx/ Optical fibers (200 mm core, NA = 0.39) Thorlabs Cat#FT200EMT 100 mL, Gastight Syringe, Small Removable Needle Hamilton Cat#1710 623 nm light LED Prizmatix Cat#Optogenetic-LED-623 1000mm, SMA-FC, Optical fiber cable Prizmatix Cat#O.G-Fiber-1000 500 mm, FC-1.25mm ferrule, Optical fiber cable Prizmatix Cat#O.G-Fiber-500-1.25 Master-8 Pulse Stimulator A.M.P.I. .. N/A Polyethylene Tubing Instech Cat#BTPE-10 e2 Neuron 113, 1–10.e1–e6, June 18, 2025 to genotype Chrnb4E61C KI mice: forward (Mf) 5’-CCAAGAGAGATTTAGGTGACAGCTGG-3’ and reverse (Mr) 5’-CCTAACTCTAGACTTTGGCCACCCC-3’ (Figure S2E).
other:
Article Title: Changes in neurotensin signalling drive hedonic devaluation in obesity.
Article Snippet: The vector was packaged into an AAV serotype 9 (ssAAV-9/2-shortCAG-dlox-mNts(rev)-dloxWPRE-SV40p(A), ~1013 infectious units per ml; produced by the Viral Vector Facility of the University of Zurich) and from the Lim laboratory (University of California San Diego, RG-EIAV-Cre).
Polymerase Chain Reaction:Article Title: The interpeduncular nucleus blunts the rewarding effect of nicotine.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SpikeSort3D software Neuralynx https://neuralynx.fh-co.com/ research-software/spikesort-3d/ Affinity Designer Serif RRID:SCR_016952 ezTrack Pennington et al.64 RRID:SCR_021496 FIJI Schindelin et al.65 RRID:SCR_002285 Other One-axis Oil Hydraulic Micromanipulator Narishige Cat#MO-10 PC-10 puller Narishige RRID:SCR_022057 Capillary micropipette for PCR 10 ml Drummond Cat#5-000-1001-X10 P-87 Flaming/Brown Micropipette Puller Sutter Instruments Cat#P87 Thin-wall borosilicate glass Warner Instruments Cat#G150TF-3 Super-Bond Universal Kit Sun Medical Cat#K058E Y-maze apparatus Imetronic https://www.imetronic.com/devices/y-maze/ Axoclamp 200B Microelectrode Amplifier Molecular Devices Cat#AxOPATCH 200B-2 Digidata 1550A Low-Noise Data Acquisition Digitizer Molecular Devices Cat#5035919A GeneClamp 500 amplifier Axon Instruments N/A 8-way, 12-way, and 2-way 4-port electric rotary valve Bio-Chem Fluidics https://www.arcmedgroup.com/pumps- valves#electric-rotary-valves SliceScope upright microscope Scientifica Cat#S-Scope-II ROLERA Bolt Scientific CMOS Camera QImaging Cat#01-ROL-BOLT-M-12 Halogen lamp unit Olympus Cat#TH4-200 Compresstome VF-200 Precisionary Instruments Cat#VF-200 uPump Microinjector with Internal Pressure Source World Precision Instruments Cat#SYS-PV820 Dual Small Animal Stereotaxic w/ Digital Display David Kopf Instruments Cat#942-WA Thomas Tetrode Mini Matrix microdrive system Thomas Recording Cat#AN000423 Tetrode for Tetrode Mini Matrix (Tip A) Thomas Recording Cat#AN000259 Quartzglass microinjection pipettes for Thomas microdrive Thomas Recording Cat#AN000155 Digital Lynx 4SX Neuralynx Cat#31-0605-0141, https://neuralynx.fh-co.com/researchhardware/data-acquisition/digital-lynx-sx/ Optical fibers (200 mm core, NA = 0.39) Thorlabs Cat#FT200EMT 100 mL, Gastight Syringe, Small Removable Needle Hamilton Cat#1710 623 nm light LED Prizmatix Cat#Optogenetic-LED-623 1000mm, SMA-FC, Optical fiber cable Prizmatix Cat#O.G-Fiber-1000 500 mm, FC-1.25mm ferrule, Optical fiber cable Prizmatix Cat#O.G-Fiber-500-1.25 Master-8 Pulse Stimulator A.M.P.I. .. N/A Polyethylene Tubing Instech Cat#BTPE-10 e2 Neuron 113, 1–10.e1–e6, June 18, 2025 to genotype Chrnb4E61C KI mice: forward (Mf) 5’-CCAAGAGAGATTTAGGTGACAGCTGG-3’ and reverse (Mr) 5’-CCTAACTCTAGACTTTGGCCACCCC-3’ (Figure S2E).
Microscopy:Article Title: The interpeduncular nucleus blunts the rewarding effect of nicotine.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SpikeSort3D software Neuralynx https://neuralynx.fh-co.com/ research-software/spikesort-3d/ Affinity Designer Serif RRID:SCR_016952 ezTrack Pennington et al.64 RRID:SCR_021496 FIJI Schindelin et al.65 RRID:SCR_002285 Other One-axis Oil Hydraulic Micromanipulator Narishige Cat#MO-10 PC-10 puller Narishige RRID:SCR_022057 Capillary micropipette for PCR 10 ml Drummond Cat#5-000-1001-X10 P-87 Flaming/Brown Micropipette Puller Sutter Instruments Cat#P87 Thin-wall borosilicate glass Warner Instruments Cat#G150TF-3 Super-Bond Universal Kit Sun Medical Cat#K058E Y-maze apparatus Imetronic https://www.imetronic.com/devices/y-maze/ Axoclamp 200B Microelectrode Amplifier Molecular Devices Cat#AxOPATCH 200B-2 Digidata 1550A Low-Noise Data Acquisition Digitizer Molecular Devices Cat#5035919A GeneClamp 500 amplifier Axon Instruments N/A 8-way, 12-way, and 2-way 4-port electric rotary valve Bio-Chem Fluidics https://www.arcmedgroup.com/pumps- valves#electric-rotary-valves SliceScope upright microscope Scientifica Cat#S-Scope-II ROLERA Bolt Scientific CMOS Camera QImaging Cat#01-ROL-BOLT-M-12 Halogen lamp unit Olympus Cat#TH4-200 Compresstome VF-200 Precisionary Instruments Cat#VF-200 uPump Microinjector with Internal Pressure Source World Precision Instruments Cat#SYS-PV820 Dual Small Animal Stereotaxic w/ Digital Display David Kopf Instruments Cat#942-WA Thomas Tetrode Mini Matrix microdrive system Thomas Recording Cat#AN000423 Tetrode for Tetrode Mini Matrix (Tip A) Thomas Recording Cat#AN000259 Quartzglass microinjection pipettes for Thomas microdrive Thomas Recording Cat#AN000155 Digital Lynx 4SX Neuralynx Cat#31-0605-0141, https://neuralynx.fh-co.com/researchhardware/data-acquisition/digital-lynx-sx/ Optical fibers (200 mm core, NA = 0.39) Thorlabs Cat#FT200EMT 100 mL, Gastight Syringe, Small Removable Needle Hamilton Cat#1710 623 nm light LED Prizmatix Cat#Optogenetic-LED-623 1000mm, SMA-FC, Optical fiber cable Prizmatix Cat#O.G-Fiber-1000 500 mm, FC-1.25mm ferrule, Optical fiber cable Prizmatix Cat#O.G-Fiber-500-1.25 Master-8 Pulse Stimulator A.M.P.I. .. N/A Polyethylene Tubing Instech Cat#BTPE-10 e2 Neuron 113, 1–10.e1–e6, June 18, 2025 to genotype Chrnb4E61C KI mice: forward (Mf) 5’-CCAAGAGAGATTTAGGTGACAGCTGG-3’ and reverse (Mr) 5’-CCTAACTCTAGACTTTGGCCACCCC-3’ (Figure S2E).
Microinjection:Article Title: The interpeduncular nucleus blunts the rewarding effect of nicotine.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SpikeSort3D software Neuralynx https://neuralynx.fh-co.com/ research-software/spikesort-3d/ Affinity Designer Serif RRID:SCR_016952 ezTrack Pennington et al.64 RRID:SCR_021496 FIJI Schindelin et al.65 RRID:SCR_002285 Other One-axis Oil Hydraulic Micromanipulator Narishige Cat#MO-10 PC-10 puller Narishige RRID:SCR_022057 Capillary micropipette for PCR 10 ml Drummond Cat#5-000-1001-X10 P-87 Flaming/Brown Micropipette Puller Sutter Instruments Cat#P87 Thin-wall borosilicate glass Warner Instruments Cat#G150TF-3 Super-Bond Universal Kit Sun Medical Cat#K058E Y-maze apparatus Imetronic https://www.imetronic.com/devices/y-maze/ Axoclamp 200B Microelectrode Amplifier Molecular Devices Cat#AxOPATCH 200B-2 Digidata 1550A Low-Noise Data Acquisition Digitizer Molecular Devices Cat#5035919A GeneClamp 500 amplifier Axon Instruments N/A 8-way, 12-way, and 2-way 4-port electric rotary valve Bio-Chem Fluidics https://www.arcmedgroup.com/pumps- valves#electric-rotary-valves SliceScope upright microscope Scientifica Cat#S-Scope-II ROLERA Bolt Scientific CMOS Camera QImaging Cat#01-ROL-BOLT-M-12 Halogen lamp unit Olympus Cat#TH4-200 Compresstome VF-200 Precisionary Instruments Cat#VF-200 uPump Microinjector with Internal Pressure Source World Precision Instruments Cat#SYS-PV820 Dual Small Animal Stereotaxic w/ Digital Display David Kopf Instruments Cat#942-WA Thomas Tetrode Mini Matrix microdrive system Thomas Recording Cat#AN000423 Tetrode for Tetrode Mini Matrix (Tip A) Thomas Recording Cat#AN000259 Quartzglass microinjection pipettes for Thomas microdrive Thomas Recording Cat#AN000155 Digital Lynx 4SX Neuralynx Cat#31-0605-0141, https://neuralynx.fh-co.com/researchhardware/data-acquisition/digital-lynx-sx/ Optical fibers (200 mm core, NA = 0.39) Thorlabs Cat#FT200EMT 100 mL, Gastight Syringe, Small Removable Needle Hamilton Cat#1710 623 nm light LED Prizmatix Cat#Optogenetic-LED-623 1000mm, SMA-FC, Optical fiber cable Prizmatix Cat#O.G-Fiber-1000 500 mm, FC-1.25mm ferrule, Optical fiber cable Prizmatix Cat#O.G-Fiber-500-1.25 Master-8 Pulse Stimulator A.M.P.I. .. N/A Polyethylene Tubing Instech Cat#BTPE-10 e2 Neuron 113, 1–10.e1–e6, June 18, 2025 to genotype Chrnb4E61C KI mice: forward (Mf) 5’-CCAAGAGAGATTTAGGTGACAGCTGG-3’ and reverse (Mr) 5’-CCTAACTCTAGACTTTGGCCACCCC-3’ (Figure S2E).
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