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3d rectangular bounding box  (Oxford Instruments)


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    Structured Review

    Oxford Instruments 3d rectangular bounding box
    Cell spreading and focal adhesion clustering are enhanced with moderate network plasticity. (A) Representative maximum intensity projection images of MSCs encapsulated within 3.6-kPa (initial modulus) alginate gels with different plasticity. Green represents actin, blue represents the nucleus, and red represents FAK kinase. Confocal images were taken after 3 d in culture. (B) Quantification of the cell aspect ratio, as defined by the <t>3D</t> <t>rectangular</t> bounding <t>box’s</t> longest side divided by its shortest side. (C) Quantification of the nuclear aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side, showing no significant difference between the conditions. (D) Cellular volume as measured per cell using Imaris Image Analysis software. (E) Representative images of focal adhesions between different binding PEG. (Scale bar, 5 µm.) (F) Histograms of focal adhesion spacing in 3D space, as based on the nearest neighbor distances for k = 4 neighbors. ANOVA analysis resulted in P < 0.001. (G) Cell spreading in gels of Young’s moduli of 1.1, 3.4, and 8 kPa, respectively, as measured by nanoindentation and varying levels of plasticity. *P < 0.1, **P < 0.01, ***P < 0.001, n.s., not significant.
    3d Rectangular Bounding Box, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+rectangular+bounding+box/Imaris/pmc07584996-86-46-74
    Average 99 stars, based on 44473 article reviews
    3d rectangular bounding box - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Extracellular matrix plasticity as a driver of cell spreading"

    Article Title: Extracellular matrix plasticity as a driver of cell spreading

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.2008801117

    Cell spreading and focal adhesion clustering are enhanced with moderate network plasticity. (A) Representative maximum intensity projection images of MSCs encapsulated within 3.6-kPa (initial modulus) alginate gels with different plasticity. Green represents actin, blue represents the nucleus, and red represents FAK kinase. Confocal images were taken after 3 d in culture. (B) Quantification of the cell aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side. (C) Quantification of the nuclear aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side, showing no significant difference between the conditions. (D) Cellular volume as measured per cell using Imaris Image Analysis software. (E) Representative images of focal adhesions between different binding PEG. (Scale bar, 5 µm.) (F) Histograms of focal adhesion spacing in 3D space, as based on the nearest neighbor distances for k = 4 neighbors. ANOVA analysis resulted in P < 0.001. (G) Cell spreading in gels of Young’s moduli of 1.1, 3.4, and 8 kPa, respectively, as measured by nanoindentation and varying levels of plasticity. *P < 0.1, **P < 0.01, ***P < 0.001, n.s., not significant.
    Figure Legend Snippet: Cell spreading and focal adhesion clustering are enhanced with moderate network plasticity. (A) Representative maximum intensity projection images of MSCs encapsulated within 3.6-kPa (initial modulus) alginate gels with different plasticity. Green represents actin, blue represents the nucleus, and red represents FAK kinase. Confocal images were taken after 3 d in culture. (B) Quantification of the cell aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side. (C) Quantification of the nuclear aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side, showing no significant difference between the conditions. (D) Cellular volume as measured per cell using Imaris Image Analysis software. (E) Representative images of focal adhesions between different binding PEG. (Scale bar, 5 µm.) (F) Histograms of focal adhesion spacing in 3D space, as based on the nearest neighbor distances for k = 4 neighbors. ANOVA analysis resulted in P < 0.001. (G) Cell spreading in gels of Young’s moduli of 1.1, 3.4, and 8 kPa, respectively, as measured by nanoindentation and varying levels of plasticity. *P < 0.1, **P < 0.01, ***P < 0.001, n.s., not significant.

    Techniques Used: Software, Binding Assay

    Related Articles

    Software:

    Article Title: Morphological plasticity facilitates lung adaptation and dynamic immune remodeling in Mycobacterium abscessus infection
    Article Snippet: .. To quantify bacterial internalization versus extracellular adherence, z-stack images (step size: 0.3 μm) were acquired and subjected to 3D volumetric reconstruction using Imaris v9.5 software. ..

    Article Title: Chrysanthemum Morifolium– Derived Nanovesicles Reduce Lactate‐Associated Histone Lactylation and Modulate Microglia‐Mediated Neuroinflammation in Sepsis‐Associated Encephalopathy
    Article Snippet: An excitation wavelength of 488 nm and emission wavelength of 519 nm were employed to visualize the Iba‐1 protein. .. Images of microglial branch nodes (1024 × 1024 pixel) and total branch length were reconstructed and quantitatively analyzed using the “Filaments” module of the Imaris 10.1.0 software. ..

    Morphology:

    Article Title: Frontal cortical neuroinflammation and circulating biomarkers following cardiopulmonary bypass in sheep.
    Article Snippet: Images were acquired using the Nikon-Crest Spinning Disk Confocal microscope, at 40× magnification (objective Plan-Apochromat, 40×/1.25, silicone), with the systemrecommended 0.35 μm step size for a z-stack size of 20.1 μm. .. Microglial morphology was analysed in Imaris v10.2 (Oxford Instruments, Abingdon, UK) [30]. ..

    other:

    Article Title: Multiple motor proteins regulate TDP-43 anterograde axonal transport.
    Article Snippet: a—top Representative micrographs of particles movement, segmentation and tracking determined with Imaris spots tracking algorithms.



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    Oxford Instruments 3d rectangular bounding box
    Cell spreading and focal adhesion clustering are enhanced with moderate network plasticity. (A) Representative maximum intensity projection images of MSCs encapsulated within 3.6-kPa (initial modulus) alginate gels with different plasticity. Green represents actin, blue represents the nucleus, and red represents FAK kinase. Confocal images were taken after 3 d in culture. (B) Quantification of the cell aspect ratio, as defined by the <t>3D</t> <t>rectangular</t> bounding <t>box’s</t> longest side divided by its shortest side. (C) Quantification of the nuclear aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side, showing no significant difference between the conditions. (D) Cellular volume as measured per cell using Imaris Image Analysis software. (E) Representative images of focal adhesions between different binding PEG. (Scale bar, 5 µm.) (F) Histograms of focal adhesion spacing in 3D space, as based on the nearest neighbor distances for k = 4 neighbors. ANOVA analysis resulted in P < 0.001. (G) Cell spreading in gels of Young’s moduli of 1.1, 3.4, and 8 kPa, respectively, as measured by nanoindentation and varying levels of plasticity. *P < 0.1, **P < 0.01, ***P < 0.001, n.s., not significant.
    3d Rectangular Bounding Box, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+rectangular+bounding+box/Imaris/pmc07584996-86-46-74
    Average 99 stars, based on 1 article reviews
    3d rectangular bounding box - by Bioz Stars, 2026-10
    99/100 stars
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    Cell spreading and focal adhesion clustering are enhanced with moderate network plasticity. (A) Representative maximum intensity projection images of MSCs encapsulated within 3.6-kPa (initial modulus) alginate gels with different plasticity. Green represents actin, blue represents the nucleus, and red represents FAK kinase. Confocal images were taken after 3 d in culture. (B) Quantification of the cell aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side. (C) Quantification of the nuclear aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side, showing no significant difference between the conditions. (D) Cellular volume as measured per cell using Imaris Image Analysis software. (E) Representative images of focal adhesions between different binding PEG. (Scale bar, 5 µm.) (F) Histograms of focal adhesion spacing in 3D space, as based on the nearest neighbor distances for k = 4 neighbors. ANOVA analysis resulted in P < 0.001. (G) Cell spreading in gels of Young’s moduli of 1.1, 3.4, and 8 kPa, respectively, as measured by nanoindentation and varying levels of plasticity. *P < 0.1, **P < 0.01, ***P < 0.001, n.s., not significant.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Extracellular matrix plasticity as a driver of cell spreading

    doi: 10.1073/pnas.2008801117

    Figure Lengend Snippet: Cell spreading and focal adhesion clustering are enhanced with moderate network plasticity. (A) Representative maximum intensity projection images of MSCs encapsulated within 3.6-kPa (initial modulus) alginate gels with different plasticity. Green represents actin, blue represents the nucleus, and red represents FAK kinase. Confocal images were taken after 3 d in culture. (B) Quantification of the cell aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side. (C) Quantification of the nuclear aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side, showing no significant difference between the conditions. (D) Cellular volume as measured per cell using Imaris Image Analysis software. (E) Representative images of focal adhesions between different binding PEG. (Scale bar, 5 µm.) (F) Histograms of focal adhesion spacing in 3D space, as based on the nearest neighbor distances for k = 4 neighbors. ANOVA analysis resulted in P < 0.001. (G) Cell spreading in gels of Young’s moduli of 1.1, 3.4, and 8 kPa, respectively, as measured by nanoindentation and varying levels of plasticity. *P < 0.1, **P < 0.01, ***P < 0.001, n.s., not significant.

    Article Snippet: Confocal images were taken after 3 d in culture. ( B ) Quantification of the cell aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side. ( C ) Quantification of the nuclear aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side, showing no significant difference between the conditions. ( D ) Cellular volume as measured per cell using Imaris Image Analysis software. ( E ) Representative images of focal adhesions between different binding PEG. (Scale bar, 5 µm.) ( F ) Histograms of focal adhesion spacing in 3D space, as based on the nearest neighbor distances for k = 4 neighbors.

    Techniques: Software, Binding Assay