3d rectangular bounding box (Oxford Instruments)
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3d Rectangular Bounding Box, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3d+rectangular+bounding+box/Imaris/pmc07584996-86-46-74
Average 99 stars, based on 44473 article reviews
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1) Product Images from "Extracellular matrix plasticity as a driver of cell spreading"
Article Title: Extracellular matrix plasticity as a driver of cell spreading
Journal: Proceedings of the National Academy of Sciences of the United States of America
doi: 10.1073/pnas.2008801117
Figure Legend Snippet: Cell spreading and focal adhesion clustering are enhanced with moderate network plasticity. (A) Representative maximum intensity projection images of MSCs encapsulated within 3.6-kPa (initial modulus) alginate gels with different plasticity. Green represents actin, blue represents the nucleus, and red represents FAK kinase. Confocal images were taken after 3 d in culture. (B) Quantification of the cell aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side. (C) Quantification of the nuclear aspect ratio, as defined by the 3D rectangular bounding box’s longest side divided by its shortest side, showing no significant difference between the conditions. (D) Cellular volume as measured per cell using Imaris Image Analysis software. (E) Representative images of focal adhesions between different binding PEG. (Scale bar, 5 µm.) (F) Histograms of focal adhesion spacing in 3D space, as based on the nearest neighbor distances for k = 4 neighbors. ANOVA analysis resulted in P < 0.001. (G) Cell spreading in gels of Young’s moduli of 1.1, 3.4, and 8 kPa, respectively, as measured by nanoindentation and varying levels of plasticity. *P < 0.1, **P < 0.01, ***P < 0.001, n.s., not significant.
Techniques Used: Software, Binding Assay
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