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addgene id 100834 aav1 chemicals  (Addgene inc)


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    Addgene inc addgene id 100834 aav1 chemicals
    Addgene Id 100834 Aav1 Chemicals, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+plot+generated+using+matlab-mathworks+2016/pENN%2EAAV%2ECamKII%2EGCaMP6f%2EWPRE%2ESV40+(Plasmid+%23100834)/pm35417688-278-49-49
    Average 95 stars, based on 135 article reviews
    addgene id 100834 aav1 chemicals - by Bioz Stars, 2026-09
    95/100 stars

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    Recombinant:

    Article Title: Motor cortex somatostatin interneurons adaptively shape the structure of action sequences.
    Article Snippet: .. Recombinant adeno-associated viral (AAV) vectors The following Cre recombinase-dependent adeno-associated viral vectors were used in the calcium imaging, optogenetic, and chemogenetic experiments: AAV1/9 CAG FLEX GCaMP6f (titre 1.9x1013, Addgene # 100836-AAV1 ), AAV9.CamKII.GCaMP6f (titre, 2.5x1013, Addgene# 100834-AAV9), AAV2-hSyn-DIO-hM4D(Gi)-mCherry (titre 1.9x1013, Addgene# 44362-AAV2), AAV1-Ef1a-DIO-eNpHR 3.0-EYFP (titre 7x1012, Addgene# 26966-AAV1), AAV1-Ef1a-DIO-eYFP (titre 2.7x1012, Addgene# 27056-AAV1). ..

    Article Title: Motor cortex somatostatin interneurons adaptively shape the structure of action sequences.
    Article Snippet: .. Recombinant adeno-associated viral (AAV) vectors The following Cre recombinase-dependent adeno-associated viral vectors were used in the calcium imaging, optogenetic, and chemogenetic experiments: AAV1/9 CAG FLEX GCaMP6f (titre 1.9x10, Addgene # 100836-AAV1 ), AAV9.CamKII.GCaMP6f (titre, 2.5x10, Addgene# 100834-AAV9), AAV2-hSyn-DIO-hM4D(Gi)-mCherry (titre 1.9x10, Addgene# 44362-AAV2), AAV1-Ef1a-DIO-eNpHR 3.0-EYFP (titre 7x10, Addgene# 26966-AAV1), AAV1-Ef1a-DIO-eYFP (titre 2.7x10, Addgene# 27056-AAV1). ..

    Article Title: Subtype-specific modulation of inhibitory interneurons by general anesthetics
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-NeuN Abcam Cat# ab177487; RRID: AB_2532109 Rabbit polyclonal anti-GABA Sigma-Aldrich Cat#A2052; RRID: AB_477652 Alexa Fluor TM 647 goat anti-rabbit IgG Thermo Fisher Scientific Cat#A-21244; RRID: AB_2535812 Bacterial and virus strains AAV1-CaMKII-GCaMP6f-WPRE-SV40 Addgene Addgene plasmid #100834; AAV1 AAV1-mDlx-GCaMP6f-Fischell-2 Addgene Addgene plasmid #83899; AAV1-CAG-flex-GCaMP6f-WPRE-SV40 Addgene Addgene plasmid #100835; Chemicals, peptides, and recombinant proteins Isoflurane Viatris Healthcare 901036504 Ketamine Daiichi Sankyo N01AX03 Xylazine Bayer N/A Propofol Fresenius Kabi Japan N/A Normal saline Otsuka N/A VectaShield with DAPI Vector Labs Cat#H-1200; RRID: AB_2336790 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Strain#000664; RRID: IMSR_JAX:000664 Mouse: Pvalb Cre Tanahira et al. 35 N/A Mouse: Sst-IRES-Cre The Jackson Laboratory Strain#013044; RRID: IMSR_JAX:013044 Software and algorithms CaImAn Giovannucci et al., 2018 http://github.com/simonsfoundation/CaImAn MATLAB_R2022a Mathworks RRID: SCR_001622 Custom code (MATLAB) to analyze data Okada et al. 36 N/A EEG/EMG data collection system Unique Medical UAS-308S SleepSign Kissei Comtech RRID: SCR_018200 ImageJ National Institutes of Health (NIH) RRID: SCR_003070 GraphPad Prism 10 GraphPad Software. .. Inc. RRID:SCR_002798 Adobe Illustrator Adobe Systems RRID: SCR_010279 Other SomnoSuite Low-Flow Anesthesia System Kent Scientific https://www.kentscientific.com/products/ somnosuite/ EEG/EMG amplifier Unique Medical EBA-100 Stereotaxic device Narishige SR-5M-HT Stereotaxic micromanipulator Narishige SM-15R, SM15M-2 Micropipette puller Narishige PC-100 Glass Capillary, 1 mm diameter Narishige GDC-1 Microinjector BEX BJ-120, BT-200 Coverslip Glass, 2- and 4.5- mm diameter Matsunami Glass N/A Multiphoton microscope Nikon A1R MP system Nikon 25× water immersion objective (1.10 NA) Nikon N/A Ti:sapphire laser Chamereon, Coherent https://www.coherent.com/lasers/oscillators/ chameleon-ultra Fluorescence microscope Keyence BZ-X700 All-in-one Fluorescence IR camera SriHome N/A e1 iScience 29, 115140, March 20, 2026

    Bioprocessing:

    Article Title: Motor cortex somatostatin interneurons adaptively shape the structure of action sequences.
    Article Snippet: .. Recombinant adeno-associated viral (AAV) vectors The following Cre recombinase-dependent adeno-associated viral vectors were used in the calcium imaging, optogenetic, and chemogenetic experiments: AAV1/9 CAG FLEX GCaMP6f (titre 1.9x1013, Addgene # 100836-AAV1 ), AAV9.CamKII.GCaMP6f (titre, 2.5x1013, Addgene# 100834-AAV9), AAV2-hSyn-DIO-hM4D(Gi)-mCherry (titre 1.9x1013, Addgene# 44362-AAV2), AAV1-Ef1a-DIO-eNpHR 3.0-EYFP (titre 7x1012, Addgene# 26966-AAV1), AAV1-Ef1a-DIO-eYFP (titre 2.7x1012, Addgene# 27056-AAV1). ..

    Article Title: Motor cortex somatostatin interneurons adaptively shape the structure of action sequences.
    Article Snippet: .. Recombinant adeno-associated viral (AAV) vectors The following Cre recombinase-dependent adeno-associated viral vectors were used in the calcium imaging, optogenetic, and chemogenetic experiments: AAV1/9 CAG FLEX GCaMP6f (titre 1.9x10, Addgene # 100836-AAV1 ), AAV9.CamKII.GCaMP6f (titre, 2.5x10, Addgene# 100834-AAV9), AAV2-hSyn-DIO-hM4D(Gi)-mCherry (titre 1.9x10, Addgene# 44362-AAV2), AAV1-Ef1a-DIO-eNpHR 3.0-EYFP (titre 7x10, Addgene# 26966-AAV1), AAV1-Ef1a-DIO-eYFP (titre 2.7x10, Addgene# 27056-AAV1). ..

    Imaging:

    Article Title: Motor cortex somatostatin interneurons adaptively shape the structure of action sequences.
    Article Snippet: .. Recombinant adeno-associated viral (AAV) vectors The following Cre recombinase-dependent adeno-associated viral vectors were used in the calcium imaging, optogenetic, and chemogenetic experiments: AAV1/9 CAG FLEX GCaMP6f (titre 1.9x1013, Addgene # 100836-AAV1 ), AAV9.CamKII.GCaMP6f (titre, 2.5x1013, Addgene# 100834-AAV9), AAV2-hSyn-DIO-hM4D(Gi)-mCherry (titre 1.9x1013, Addgene# 44362-AAV2), AAV1-Ef1a-DIO-eNpHR 3.0-EYFP (titre 7x1012, Addgene# 26966-AAV1), AAV1-Ef1a-DIO-eYFP (titre 2.7x1012, Addgene# 27056-AAV1). ..

    Article Title: Learned and inferred valence arise from interactions between stable and dynamic subnetworks
    Article Snippet: .. For calcium imaging experiments, three injections (150 nL each) of AAV1-CaMKIIα-GCaMP6f-WPRE-SV40 (Addgene cat. # 100834) were delivered into the PL at the following stereotaxic coordinates relative to bregma: AP +1.95 mm, ML ±0.3 mm, and DV −1.7, −1.5, and −1.3 mm. ..

    Article Title: Motor cortex somatostatin interneurons adaptively shape the structure of action sequences.
    Article Snippet: .. Recombinant adeno-associated viral (AAV) vectors The following Cre recombinase-dependent adeno-associated viral vectors were used in the calcium imaging, optogenetic, and chemogenetic experiments: AAV1/9 CAG FLEX GCaMP6f (titre 1.9x10, Addgene # 100836-AAV1 ), AAV9.CamKII.GCaMP6f (titre, 2.5x10, Addgene# 100834-AAV9), AAV2-hSyn-DIO-hM4D(Gi)-mCherry (titre 1.9x10, Addgene# 44362-AAV2), AAV1-Ef1a-DIO-eNpHR 3.0-EYFP (titre 7x10, Addgene# 26966-AAV1), AAV1-Ef1a-DIO-eYFP (titre 2.7x10, Addgene# 27056-AAV1). ..

    Virus:

    Article Title: Hierarchical Summary Statistics Encoding Across Primary Visual and Posterior Parietal Cortices.
    Article Snippet: .. A virus expressing GCaMP6f (pENN.AAV.CaMKII.GCaMP6f.WPRE.SV40; Addgene Cat. #100834-AAV1; RRID: Addgene_100834) was injected into the target region (100–150 nL, 1 nL/s, 200 mm deep from the dura) using glass pipettes connected to a hydraulic injection system (Nanoject III, Drummond Scientific, Broomall, PA, USA). ..

    Article Title: Subtype-specific modulation of inhibitory interneurons by general anesthetics
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-NeuN Abcam Cat# ab177487; RRID: AB_2532109 Rabbit polyclonal anti-GABA Sigma-Aldrich Cat#A2052; RRID: AB_477652 Alexa Fluor TM 647 goat anti-rabbit IgG Thermo Fisher Scientific Cat#A-21244; RRID: AB_2535812 Bacterial and virus strains AAV1-CaMKII-GCaMP6f-WPRE-SV40 Addgene Addgene plasmid #100834; AAV1 AAV1-mDlx-GCaMP6f-Fischell-2 Addgene Addgene plasmid #83899; AAV1-CAG-flex-GCaMP6f-WPRE-SV40 Addgene Addgene plasmid #100835; Chemicals, peptides, and recombinant proteins Isoflurane Viatris Healthcare 901036504 Ketamine Daiichi Sankyo N01AX03 Xylazine Bayer N/A Propofol Fresenius Kabi Japan N/A Normal saline Otsuka N/A VectaShield with DAPI Vector Labs Cat#H-1200; RRID: AB_2336790 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Strain#000664; RRID: IMSR_JAX:000664 Mouse: Pvalb Cre Tanahira et al. 35 N/A Mouse: Sst-IRES-Cre The Jackson Laboratory Strain#013044; RRID: IMSR_JAX:013044 Software and algorithms CaImAn Giovannucci et al., 2018 http://github.com/simonsfoundation/CaImAn MATLAB_R2022a Mathworks RRID: SCR_001622 Custom code (MATLAB) to analyze data Okada et al. 36 N/A EEG/EMG data collection system Unique Medical UAS-308S SleepSign Kissei Comtech RRID: SCR_018200 ImageJ National Institutes of Health (NIH) RRID: SCR_003070 GraphPad Prism 10 GraphPad Software. .. Inc. RRID:SCR_002798 Adobe Illustrator Adobe Systems RRID: SCR_010279 Other SomnoSuite Low-Flow Anesthesia System Kent Scientific https://www.kentscientific.com/products/ somnosuite/ EEG/EMG amplifier Unique Medical EBA-100 Stereotaxic device Narishige SR-5M-HT Stereotaxic micromanipulator Narishige SM-15R, SM15M-2 Micropipette puller Narishige PC-100 Glass Capillary, 1 mm diameter Narishige GDC-1 Microinjector BEX BJ-120, BT-200 Coverslip Glass, 2- and 4.5- mm diameter Matsunami Glass N/A Multiphoton microscope Nikon A1R MP system Nikon 25× water immersion objective (1.10 NA) Nikon N/A Ti:sapphire laser Chamereon, Coherent https://www.coherent.com/lasers/oscillators/ chameleon-ultra Fluorescence microscope Keyence BZ-X700 All-in-one Fluorescence IR camera SriHome N/A e1 iScience 29, 115140, March 20, 2026

    Expressing:

    Article Title: Hierarchical Summary Statistics Encoding Across Primary Visual and Posterior Parietal Cortices.
    Article Snippet: .. A virus expressing GCaMP6f (pENN.AAV.CaMKII.GCaMP6f.WPRE.SV40; Addgene Cat. #100834-AAV1; RRID: Addgene_100834) was injected into the target region (100–150 nL, 1 nL/s, 200 mm deep from the dura) using glass pipettes connected to a hydraulic injection system (Nanoject III, Drummond Scientific, Broomall, PA, USA). ..

    Article Title: Mediodorsal thalamus of alcohol-dependent mice shows genetic and physiological adaptations and alcohol-biased calcium signaling.
    Article Snippet: .. To induce expression of a calcium biosensor, a viral vector construct for expression of GCaMP6f (pENN.AAV1.CamKII.GCaMP6f.WPRE.SV40; Addgene cat# 100834) was unilaterally injected in MD (200 μL; 50 μL/ min; AP: 1.58 mm, ML: 0.44 mm, DV: 3.2 mm, relative to Bregma) followed by placement of a 2.5 mm fiber optic ferrule containing a 400 μm optical fiber (0.48 NA) above the injection site coordinates (AP: 1.58 mm, ML: 0.44 mm, DV: 3.1 mm). ..

    Injection:

    Article Title: Hierarchical Summary Statistics Encoding Across Primary Visual and Posterior Parietal Cortices.
    Article Snippet: .. A virus expressing GCaMP6f (pENN.AAV.CaMKII.GCaMP6f.WPRE.SV40; Addgene Cat. #100834-AAV1; RRID: Addgene_100834) was injected into the target region (100–150 nL, 1 nL/s, 200 mm deep from the dura) using glass pipettes connected to a hydraulic injection system (Nanoject III, Drummond Scientific, Broomall, PA, USA). ..

    Article Title: Mediodorsal thalamus of alcohol-dependent mice shows genetic and physiological adaptations and alcohol-biased calcium signaling.
    Article Snippet: .. To induce expression of a calcium biosensor, a viral vector construct for expression of GCaMP6f (pENN.AAV1.CamKII.GCaMP6f.WPRE.SV40; Addgene cat# 100834) was unilaterally injected in MD (200 μL; 50 μL/ min; AP: 1.58 mm, ML: 0.44 mm, DV: 3.2 mm, relative to Bregma) followed by placement of a 2.5 mm fiber optic ferrule containing a 400 μm optical fiber (0.48 NA) above the injection site coordinates (AP: 1.58 mm, ML: 0.44 mm, DV: 3.1 mm). ..

    Plasmid Preparation:

    Article Title: Mediodorsal thalamus of alcohol-dependent mice shows genetic and physiological adaptations and alcohol-biased calcium signaling.
    Article Snippet: .. To induce expression of a calcium biosensor, a viral vector construct for expression of GCaMP6f (pENN.AAV1.CamKII.GCaMP6f.WPRE.SV40; Addgene cat# 100834) was unilaterally injected in MD (200 μL; 50 μL/ min; AP: 1.58 mm, ML: 0.44 mm, DV: 3.2 mm, relative to Bregma) followed by placement of a 2.5 mm fiber optic ferrule containing a 400 μm optical fiber (0.48 NA) above the injection site coordinates (AP: 1.58 mm, ML: 0.44 mm, DV: 3.1 mm). ..

    Article Title: Subtype-specific modulation of inhibitory interneurons by general anesthetics
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-NeuN Abcam Cat# ab177487; RRID: AB_2532109 Rabbit polyclonal anti-GABA Sigma-Aldrich Cat#A2052; RRID: AB_477652 Alexa Fluor TM 647 goat anti-rabbit IgG Thermo Fisher Scientific Cat#A-21244; RRID: AB_2535812 Bacterial and virus strains AAV1-CaMKII-GCaMP6f-WPRE-SV40 Addgene Addgene plasmid #100834; AAV1 AAV1-mDlx-GCaMP6f-Fischell-2 Addgene Addgene plasmid #83899; AAV1-CAG-flex-GCaMP6f-WPRE-SV40 Addgene Addgene plasmid #100835; Chemicals, peptides, and recombinant proteins Isoflurane Viatris Healthcare 901036504 Ketamine Daiichi Sankyo N01AX03 Xylazine Bayer N/A Propofol Fresenius Kabi Japan N/A Normal saline Otsuka N/A VectaShield with DAPI Vector Labs Cat#H-1200; RRID: AB_2336790 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Strain#000664; RRID: IMSR_JAX:000664 Mouse: Pvalb Cre Tanahira et al. 35 N/A Mouse: Sst-IRES-Cre The Jackson Laboratory Strain#013044; RRID: IMSR_JAX:013044 Software and algorithms CaImAn Giovannucci et al., 2018 http://github.com/simonsfoundation/CaImAn MATLAB_R2022a Mathworks RRID: SCR_001622 Custom code (MATLAB) to analyze data Okada et al. 36 N/A EEG/EMG data collection system Unique Medical UAS-308S SleepSign Kissei Comtech RRID: SCR_018200 ImageJ National Institutes of Health (NIH) RRID: SCR_003070 GraphPad Prism 10 GraphPad Software. .. Inc. RRID:SCR_002798 Adobe Illustrator Adobe Systems RRID: SCR_010279 Other SomnoSuite Low-Flow Anesthesia System Kent Scientific https://www.kentscientific.com/products/ somnosuite/ EEG/EMG amplifier Unique Medical EBA-100 Stereotaxic device Narishige SR-5M-HT Stereotaxic micromanipulator Narishige SM-15R, SM15M-2 Micropipette puller Narishige PC-100 Glass Capillary, 1 mm diameter Narishige GDC-1 Microinjector BEX BJ-120, BT-200 Coverslip Glass, 2- and 4.5- mm diameter Matsunami Glass N/A Multiphoton microscope Nikon A1R MP system Nikon 25× water immersion objective (1.10 NA) Nikon N/A Ti:sapphire laser Chamereon, Coherent https://www.coherent.com/lasers/oscillators/ chameleon-ultra Fluorescence microscope Keyence BZ-X700 All-in-one Fluorescence IR camera SriHome N/A e1 iScience 29, 115140, March 20, 2026

    Article Title: Subtype-specific modulation of inhibitory interneurons by general anesthetics
    Article Snippet: .. AAV1-CaMKII-GCaMP6f-WPRE-SV40 , Addgene , Addgene plasmid #100834; AAV1. .. AAV1-mDlx-GCaMP6f-Fischell-2 , Addgene , Addgene plasmid #83899;.

    Construct:

    Article Title: Mediodorsal thalamus of alcohol-dependent mice shows genetic and physiological adaptations and alcohol-biased calcium signaling.
    Article Snippet: .. To induce expression of a calcium biosensor, a viral vector construct for expression of GCaMP6f (pENN.AAV1.CamKII.GCaMP6f.WPRE.SV40; Addgene cat# 100834) was unilaterally injected in MD (200 μL; 50 μL/ min; AP: 1.58 mm, ML: 0.44 mm, DV: 3.2 mm, relative to Bregma) followed by placement of a 2.5 mm fiber optic ferrule containing a 400 μm optical fiber (0.48 NA) above the injection site coordinates (AP: 1.58 mm, ML: 0.44 mm, DV: 3.1 mm). ..

    Saline:

    Article Title: Subtype-specific modulation of inhibitory interneurons by general anesthetics
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-NeuN Abcam Cat# ab177487; RRID: AB_2532109 Rabbit polyclonal anti-GABA Sigma-Aldrich Cat#A2052; RRID: AB_477652 Alexa Fluor TM 647 goat anti-rabbit IgG Thermo Fisher Scientific Cat#A-21244; RRID: AB_2535812 Bacterial and virus strains AAV1-CaMKII-GCaMP6f-WPRE-SV40 Addgene Addgene plasmid #100834; AAV1 AAV1-mDlx-GCaMP6f-Fischell-2 Addgene Addgene plasmid #83899; AAV1-CAG-flex-GCaMP6f-WPRE-SV40 Addgene Addgene plasmid #100835; Chemicals, peptides, and recombinant proteins Isoflurane Viatris Healthcare 901036504 Ketamine Daiichi Sankyo N01AX03 Xylazine Bayer N/A Propofol Fresenius Kabi Japan N/A Normal saline Otsuka N/A VectaShield with DAPI Vector Labs Cat#H-1200; RRID: AB_2336790 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Strain#000664; RRID: IMSR_JAX:000664 Mouse: Pvalb Cre Tanahira et al. 35 N/A Mouse: Sst-IRES-Cre The Jackson Laboratory Strain#013044; RRID: IMSR_JAX:013044 Software and algorithms CaImAn Giovannucci et al., 2018 http://github.com/simonsfoundation/CaImAn MATLAB_R2022a Mathworks RRID: SCR_001622 Custom code (MATLAB) to analyze data Okada et al. 36 N/A EEG/EMG data collection system Unique Medical UAS-308S SleepSign Kissei Comtech RRID: SCR_018200 ImageJ National Institutes of Health (NIH) RRID: SCR_003070 GraphPad Prism 10 GraphPad Software. .. Inc. RRID:SCR_002798 Adobe Illustrator Adobe Systems RRID: SCR_010279 Other SomnoSuite Low-Flow Anesthesia System Kent Scientific https://www.kentscientific.com/products/ somnosuite/ EEG/EMG amplifier Unique Medical EBA-100 Stereotaxic device Narishige SR-5M-HT Stereotaxic micromanipulator Narishige SM-15R, SM15M-2 Micropipette puller Narishige PC-100 Glass Capillary, 1 mm diameter Narishige GDC-1 Microinjector BEX BJ-120, BT-200 Coverslip Glass, 2- and 4.5- mm diameter Matsunami Glass N/A Multiphoton microscope Nikon A1R MP system Nikon 25× water immersion objective (1.10 NA) Nikon N/A Ti:sapphire laser Chamereon, Coherent https://www.coherent.com/lasers/oscillators/ chameleon-ultra Fluorescence microscope Keyence BZ-X700 All-in-one Fluorescence IR camera SriHome N/A e1 iScience 29, 115140, March 20, 2026



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