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3d immersive visualization program  (syGlass Inc)

 
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    syGlass Inc 3d immersive visualization program
    Experimental design for cell counting using <t>3D</t> virtual reality. (A) Serial sections containing MNTB from Cre;Ai9 mice were stained with cell-specific antibodies for neurons (Map2), astrocytes (Aldh1L1), oligodendrocytes (Sox10) and microglia (Iba1), in an alternating manner, along with DAPI. These slices were imaged with a 40× objective and tiled. The MNTB is outlined in white in each section and the midline is indicated by a dashed white line. Scale bar = 100 μm. (B) The MNTB was cropped from the larger image and imported <t>into</t> <t>syGlass</t> for cell counting in 3D virtual reality. The solid and dashed white lines demarcate cut planes that were rotated to generate the 3D view in C (arrow). Scale bar = 50 μm. (C) Rotated view of part of MNTB to illustrate 3D view for cell counting. Counting dots were placed onto the nuclei. White dots indicate incomplete (sectioned) nuclei in the first slice of the z-stack. These were excluded from analysis, but incomplete nuclei in the bottom slice were counted and included in the analysis. Red dots indicate cells that were counted. Note that cells throughout the entire MNTB (panel B) were counted.
    3d Immersive Visualization Program, supplied by syGlass Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+immersive+visualization+program/virtual+reality+software/pmc09245928-234-2-1
    Average 90 stars, based on 1 article reviews
    3d immersive visualization program - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Glial Cell Expansion Coincides with Neural Circuit Formation in the Developing Auditory Brainstem"

    Article Title: Glial Cell Expansion Coincides with Neural Circuit Formation in the Developing Auditory Brainstem

    Journal: Developmental neurobiology

    doi: 10.1002/dneu.22633

    Experimental design for cell counting using 3D virtual reality. (A) Serial sections containing MNTB from Cre;Ai9 mice were stained with cell-specific antibodies for neurons (Map2), astrocytes (Aldh1L1), oligodendrocytes (Sox10) and microglia (Iba1), in an alternating manner, along with DAPI. These slices were imaged with a 40× objective and tiled. The MNTB is outlined in white in each section and the midline is indicated by a dashed white line. Scale bar = 100 μm. (B) The MNTB was cropped from the larger image and imported into syGlass for cell counting in 3D virtual reality. The solid and dashed white lines demarcate cut planes that were rotated to generate the 3D view in C (arrow). Scale bar = 50 μm. (C) Rotated view of part of MNTB to illustrate 3D view for cell counting. Counting dots were placed onto the nuclei. White dots indicate incomplete (sectioned) nuclei in the first slice of the z-stack. These were excluded from analysis, but incomplete nuclei in the bottom slice were counted and included in the analysis. Red dots indicate cells that were counted. Note that cells throughout the entire MNTB (panel B) were counted.
    Figure Legend Snippet: Experimental design for cell counting using 3D virtual reality. (A) Serial sections containing MNTB from Cre;Ai9 mice were stained with cell-specific antibodies for neurons (Map2), astrocytes (Aldh1L1), oligodendrocytes (Sox10) and microglia (Iba1), in an alternating manner, along with DAPI. These slices were imaged with a 40× objective and tiled. The MNTB is outlined in white in each section and the midline is indicated by a dashed white line. Scale bar = 100 μm. (B) The MNTB was cropped from the larger image and imported into syGlass for cell counting in 3D virtual reality. The solid and dashed white lines demarcate cut planes that were rotated to generate the 3D view in C (arrow). Scale bar = 50 μm. (C) Rotated view of part of MNTB to illustrate 3D view for cell counting. Counting dots were placed onto the nuclei. White dots indicate incomplete (sectioned) nuclei in the first slice of the z-stack. These were excluded from analysis, but incomplete nuclei in the bottom slice were counted and included in the analysis. Red dots indicate cells that were counted. Note that cells throughout the entire MNTB (panel B) were counted.

    Techniques Used: Cell Counting, Staining

    Related Articles

    other:

    Article Title: Organization and development of bilateral somatosensory feedback projections in mice
    Article Snippet: The low density of labeling enabled the reconstruction of the complete axonal trajectories from the cortex to the brainstem, using virtual reality-assisted tracing (SyGlass, IstoVision) ( ).

    Article Title: Organization and development of bilateral somatosensory feedback projections in mice
    Article Snippet: The low density of labeling enabled the reconstruction of the complete axonal trajectories from the cortex to the brainstem, using virtual reality-assisted tracing (SyGlass, IstoVision) (Video S1).

    Article Title: Brain vasculature accumulates tau and is spatially related to tau tangle pathology in Alzheimer’s disease
    Article Snippet: The virtual reality (VR) image analysis software Syglass was used to manually segment individual blood vessels from each sample by tracing masks along the surface of each blood vessel (Fig. a).

    Article Title: Organization and development of bilateral somatosensory feedback projections in mice
    Article Snippet: Single axon reconstructions were done manually using virtual reality-assisted tracing (SyGlass, IstoVision).

    Software:

    Article Title: Brain vasculature accumulates tau and is spatially related to tau tangle pathology in Alzheimer’s disease
    Article Snippet: .. Individual blood vessels were manually segmented from clear brain images using a virtual reality image analysis software (Syglass). ..

    Article Title: Computational Methods for Image Analysis in Craniofacial Development and Disease.
    Article Snippet: Observation is at the center of all biological sciences.. Advances in imaging technologies are therefore essential to derive novel biological insights to better understand the complex workings of living systems.. Recent high-throughput sequencing and imaging techniques are allowing researchers to simultaneously address complex molecular variations spatially and temporarily in tissues and organs.

    Article Title: Virtual reality-empowered deep-learning analysis of brain cells
    Article Snippet: .. We used two commercial VR annotation software packages (Arivis VisionVR and syGlass ) to evaluate the speed and accuracy of VR in comparison to 2D slice-based annotation in ITK-SNAP. ..

    Article Title: A tridimensional atlas of the developing human head.
    Article Snippet: To isolate a specific structure in Imaris, we used the surface tool with manual selection, and then used the surface to mask the image. .. To facilitate the segmentation of anatomical structures, we used a virtual reality software syGlass coupled to an Oculus Quest2 (Meta) headset. ..

    Comparison:

    Article Title: Virtual reality-empowered deep-learning analysis of brain cells
    Article Snippet: .. We used two commercial VR annotation software packages (Arivis VisionVR and syGlass ) to evaluate the speed and accuracy of VR in comparison to 2D slice-based annotation in ITK-SNAP. ..



    Similar Products

    3d immersive visualization program  (syGlass Inc)
    90
    syGlass Inc 3d immersive visualization program
    Experimental design for cell counting using <t>3D</t> virtual reality. (A) Serial sections containing MNTB from Cre;Ai9 mice were stained with cell-specific antibodies for neurons (Map2), astrocytes (Aldh1L1), oligodendrocytes (Sox10) and microglia (Iba1), in an alternating manner, along with DAPI. These slices were imaged with a 40× objective and tiled. The MNTB is outlined in white in each section and the midline is indicated by a dashed white line. Scale bar = 100 μm. (B) The MNTB was cropped from the larger image and imported <t>into</t> <t>syGlass</t> for cell counting in 3D virtual reality. The solid and dashed white lines demarcate cut planes that were rotated to generate the 3D view in C (arrow). Scale bar = 50 μm. (C) Rotated view of part of MNTB to illustrate 3D view for cell counting. Counting dots were placed onto the nuclei. White dots indicate incomplete (sectioned) nuclei in the first slice of the z-stack. These were excluded from analysis, but incomplete nuclei in the bottom slice were counted and included in the analysis. Red dots indicate cells that were counted. Note that cells throughout the entire MNTB (panel B) were counted.
    3d Immersive Visualization Program, supplied by syGlass Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+immersive+visualization+program/virtual+reality+software/pmc09245928-234-2-1
    Average 90 stars, based on 1 article reviews
    3d immersive visualization program - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Experimental design for cell counting using 3D virtual reality. (A) Serial sections containing MNTB from Cre;Ai9 mice were stained with cell-specific antibodies for neurons (Map2), astrocytes (Aldh1L1), oligodendrocytes (Sox10) and microglia (Iba1), in an alternating manner, along with DAPI. These slices were imaged with a 40× objective and tiled. The MNTB is outlined in white in each section and the midline is indicated by a dashed white line. Scale bar = 100 μm. (B) The MNTB was cropped from the larger image and imported into syGlass for cell counting in 3D virtual reality. The solid and dashed white lines demarcate cut planes that were rotated to generate the 3D view in C (arrow). Scale bar = 50 μm. (C) Rotated view of part of MNTB to illustrate 3D view for cell counting. Counting dots were placed onto the nuclei. White dots indicate incomplete (sectioned) nuclei in the first slice of the z-stack. These were excluded from analysis, but incomplete nuclei in the bottom slice were counted and included in the analysis. Red dots indicate cells that were counted. Note that cells throughout the entire MNTB (panel B) were counted.

    Journal: Developmental neurobiology

    Article Title: Glial Cell Expansion Coincides with Neural Circuit Formation in the Developing Auditory Brainstem

    doi: 10.1002/dneu.22633

    Figure Lengend Snippet: Experimental design for cell counting using 3D virtual reality. (A) Serial sections containing MNTB from Cre;Ai9 mice were stained with cell-specific antibodies for neurons (Map2), astrocytes (Aldh1L1), oligodendrocytes (Sox10) and microglia (Iba1), in an alternating manner, along with DAPI. These slices were imaged with a 40× objective and tiled. The MNTB is outlined in white in each section and the midline is indicated by a dashed white line. Scale bar = 100 μm. (B) The MNTB was cropped from the larger image and imported into syGlass for cell counting in 3D virtual reality. The solid and dashed white lines demarcate cut planes that were rotated to generate the 3D view in C (arrow). Scale bar = 50 μm. (C) Rotated view of part of MNTB to illustrate 3D view for cell counting. Counting dots were placed onto the nuclei. White dots indicate incomplete (sectioned) nuclei in the first slice of the z-stack. These were excluded from analysis, but incomplete nuclei in the bottom slice were counted and included in the analysis. Red dots indicate cells that were counted. Note that cells throughout the entire MNTB (panel B) were counted.

    Article Snippet: The syGlass 3D immersive visualization program was used to perform cell counts because it enables rapid and accurate assessment of sectioned DAPI-labeled nuclei at the edge of the first image plane , and it permits accurate assessment of spatial association of markers with DAPI in three dimensions.

    Techniques: Cell Counting, Staining