321 tag codons (Addgene inc)

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Addgene inc 321 tag codons
Bacterial strains and plasmids used in this study.

321 Tag Codons, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/321+tag+codons/C321%2E%CE%94A+(Bacterial+strain+%2348998)/pmc08511705-2-23-30
Average 93 stars, based on 32 article reviews

321 tag codons - by Bioz Stars, 2026-11

93/100 stars

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1) Product Images from "Comparative Analyses of the Transcriptome and Proteome of Escherichia coli C321.△A and Further Improving Its Noncanonical Amino Acids Containing Protein Expression Ability by Integration of T7 RNA Polymerase"

Article Title: Comparative Analyses of the Transcriptome and Proteome of Escherichia coli C321.△A and Further Improving Its Noncanonical Amino Acids Containing Protein Expression Ability by Integration of T7 RNA Polymerase

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2021.744284

Figure Legend Snippet: Bacterial strains and plasmids used in this study.

Techniques Used: Plasmid Preparation, CRISPR, Sequencing, Mutagenesis

other:

Article Title: Functionally modified polypeptides and radiobiosynthesis
Article Snippet: In some aspects, the E. coli cell to be engineered is C321.ΔA (available as strain 48998 from Addgene; Cambridge, MA).

Article Title: Turning the band alignment of carbon dots for visible-light-driven enzymatic asymmetric reduction of aromatic ketone
Article Snippet: Keto reductases are crucial NAD(P)H-dependent enzymes used for the enantioselective synthesis of alcohols from prochiral ketones.. Typically, the NADPH cofactor is regenerated through a second enzyme and/or substrate.. However, photocatalytic cofactor regeneration using water as a sacrificial electron and hydrogen donor presents a promising alternative, albeit a challenging one.

Article Title: Improving Catalytic Activity, Acid-Tolerance, and Thermal Stability of Glutathione Peroxidase by Systematic Site-Directed Selenocysteine Incorporation.
Article Snippet: Glutathione peroxidase (GPx) is an important antioxidant enzyme.. Selenocysteine (Sec)-containing GPxs (Sec-GPxs) are usually superior to their conventional cysteine-containing counterparts (Cys-GPxs), which make up the majority of the natural GPxs but display unsuitable activity and stability for industrial applications.. This study first heterologously expressed and characterized a Cys-GPx from Lactococcus lactis (LlGPx), systematically exchanged all the three Cys to Sec and introduced an extra Sec.

Bacteria:

Article Title: STARD3 regulates lysosome positioning and contacts via a GSK3-controlled phosphorylation switch.
Article Snippet: The fractions with ~100% pure CSTD3 construct were pooled, concentrated, and supplemented with 10% (v/v) pure glycerol (Sigma). .. CSTD3 pS209 and CSTD3 pS213 were expressed in E. coli C321 ΔA bacteria [gift from George Church; Addgene # 48998 (Lajoie et al, 2013)] as described in Di Mattia et al, 2020a. .. Protein production was performed in auto-inducible medium (Formedium) supplemented with ampicillin (100 μg/mL), kanamycin (20 μg/mL), and 20 mM Ophospho-L-serine (P0878, Sigma) for 48 h at 20 °C.

Article Title: STARD3 regulates lysosome positioning and contacts via a GSK3-controlled phosphorylation switch
Article Snippet: .. C STD3 pS209 and C STD3 pS213 were expressed in E. coli C321 ΔA bacteria [gift from George Church; Addgene # 48998 (Lajoie et al, )] as described in Di Mattia et al, . .. Protein production was performed in auto-inducible medium (Formedium) supplemented with ampicillin (100 μg/mL), kanamycin (20 μg/mL), and 20 mM O-phospho-L-serine (P0878, Sigma) for 48 h at 20 °C.

Article Title: STARD3 regulates lysosome positioning and contacts via a GSK3-controlled phosphorylation switch
Article Snippet: Spectrophotometer , Safas monaco , UVmc2. .. E. coli C321 ΔA bacteria , (Lajoie et al, ) , Addgene # 48998. .. E. coli Rosetta2 (DE3) pLysS , Sigma-Aldrich , 71403.

Derivative Assay:

Article Title: Genomically recoded Escherichia coli with optimized functional phenotypes
Article Snippet: .. All strains engineered in this study are derived from Escherichia coli EcNR2 (Addgene ID 26931) and C321.ΔA (Addgene ID 48998). ..

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