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quantseq 3′ end labeling kit  (Lexogen GmbH)

 
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    Structured Review

    Lexogen GmbH quantseq 3′ end labeling kit
    KEY RESOURCES TABLE
    Quantseq 3′ End Labeling Kit, supplied by Lexogen GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3+end/quantseq+3++end+labeling+kit/pmc07842189-53-0-6
    Average 90 stars, based on 1 article reviews
    quantseq 3′ end labeling kit - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression"

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression

    Journal: Cell reports

    doi: 10.1016/j.celrep.2020.108398

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Control, Recombinant, Derivative Assay, Protease Inhibitor, End Labeling, Enzyme-linked Immunosorbent Assay, Microarray, Knock-Out, In Vivo, In Vitro, Isolation, Infection, Sequencing, Software, Binding Assay

    Related Articles

    End Labeling:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    Control:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    Recombinant:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    Derivative Assay:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    Protease Inhibitor:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    Microarray:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    Knock-Out:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    In Vivo:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    In Vitro:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    Isolation:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    Infection:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    Sequencing:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    Software:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    Binding Assay:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.

    RNA Sequencing:

    Article Title: Tau Pathology Drives Dementia Risk-Associated Gene Networks toward Chronic Inflammatory States and Immunosuppression
    Article Snippet: QuantSeq 3′ End Labeling Kit , Lexogen , Cat# 015.96.



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    A) Fluorescence microscopy images of preassembled equimolar condensates showing reciprocal recruitment of HOTAIR 1-360 and AR LBD . Upper: DyLight488-labeled AR NTD and Cy5-labeled AR LBD droplets after addition of 25 μM <t>Cy3-labeled</t> HOTAIR 1-360 . Lower: DyLight488-labeled AR NTD and Cy3-labeled HOTAIR 1-360 droplets after addition of 20 μM Cy5-labeled AR LBD . An equimolar mixture of Cy5-labeled AR LBD and Cy3-labeled HOTAIR 1-360 (25 μM each) did not undergo LLPS. Scale bar, 10 μm. Representative of three biological replicates. B) Quantification of Cy3-labeled HOTAIR 1-360 enrichment in preassembled AR NTD -HOTAIR 1-360 droplets before and after the addition of 20 μM Cy5-labeled AR LBD . C) Quantification of Cy5-labeled AR LBD enrichment in preassembled AR NTD -AR LBD droplets before and after the addition of 25 μM Cy3-labeled HOTAIR 1-360 . D) Quantification of Cy5-labeled AR LBD enrichment in preassembled AR NTD -AR LBD droplets before and after the addition of an equivalent amount of PolyU-20nt. For B-D, enrichment was calculated from raw-image fluorescence measurements. Boxes indicate quartiles and the mean; dots represent individual droplets across three biological replicates. Representative images are shown with identical linear scaling within each channel across conditions. E-G) Fluorescence microscopy images from three biological replicates of preassembled equimolar DyLight488-labeled AR NTD and Cy5-labeled AR LBD droplets after the addition of E) 400 μM SLNCR1-20nt, F) HOTAIR-20nt, or G) PolyU-20nt. Scale bar, 10 μm. The corresponding normalized line-scan intensity profiles are shown.
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    Image Search Results


    A) Fluorescence microscopy images of preassembled equimolar condensates showing reciprocal recruitment of HOTAIR 1-360 and AR LBD . Upper: DyLight488-labeled AR NTD and Cy5-labeled AR LBD droplets after addition of 25 μM Cy3-labeled HOTAIR 1-360 . Lower: DyLight488-labeled AR NTD and Cy3-labeled HOTAIR 1-360 droplets after addition of 20 μM Cy5-labeled AR LBD . An equimolar mixture of Cy5-labeled AR LBD and Cy3-labeled HOTAIR 1-360 (25 μM each) did not undergo LLPS. Scale bar, 10 μm. Representative of three biological replicates. B) Quantification of Cy3-labeled HOTAIR 1-360 enrichment in preassembled AR NTD -HOTAIR 1-360 droplets before and after the addition of 20 μM Cy5-labeled AR LBD . C) Quantification of Cy5-labeled AR LBD enrichment in preassembled AR NTD -AR LBD droplets before and after the addition of 25 μM Cy3-labeled HOTAIR 1-360 . D) Quantification of Cy5-labeled AR LBD enrichment in preassembled AR NTD -AR LBD droplets before and after the addition of an equivalent amount of PolyU-20nt. For B-D, enrichment was calculated from raw-image fluorescence measurements. Boxes indicate quartiles and the mean; dots represent individual droplets across three biological replicates. Representative images are shown with identical linear scaling within each channel across conditions. E-G) Fluorescence microscopy images from three biological replicates of preassembled equimolar DyLight488-labeled AR NTD and Cy5-labeled AR LBD droplets after the addition of E) 400 μM SLNCR1-20nt, F) HOTAIR-20nt, or G) PolyU-20nt. Scale bar, 10 μm. The corresponding normalized line-scan intensity profiles are shown.

    Journal:

    Article Title:

    doi:

    Figure Lengend Snippet: A) Fluorescence microscopy images of preassembled equimolar condensates showing reciprocal recruitment of HOTAIR 1-360 and AR LBD . Upper: DyLight488-labeled AR NTD and Cy5-labeled AR LBD droplets after addition of 25 μM Cy3-labeled HOTAIR 1-360 . Lower: DyLight488-labeled AR NTD and Cy3-labeled HOTAIR 1-360 droplets after addition of 20 μM Cy5-labeled AR LBD . An equimolar mixture of Cy5-labeled AR LBD and Cy3-labeled HOTAIR 1-360 (25 μM each) did not undergo LLPS. Scale bar, 10 μm. Representative of three biological replicates. B) Quantification of Cy3-labeled HOTAIR 1-360 enrichment in preassembled AR NTD -HOTAIR 1-360 droplets before and after the addition of 20 μM Cy5-labeled AR LBD . C) Quantification of Cy5-labeled AR LBD enrichment in preassembled AR NTD -AR LBD droplets before and after the addition of 25 μM Cy3-labeled HOTAIR 1-360 . D) Quantification of Cy5-labeled AR LBD enrichment in preassembled AR NTD -AR LBD droplets before and after the addition of an equivalent amount of PolyU-20nt. For B-D, enrichment was calculated from raw-image fluorescence measurements. Boxes indicate quartiles and the mean; dots represent individual droplets across three biological replicates. Representative images are shown with identical linear scaling within each channel across conditions. E-G) Fluorescence microscopy images from three biological replicates of preassembled equimolar DyLight488-labeled AR NTD and Cy5-labeled AR LBD droplets after the addition of E) 400 μM SLNCR1-20nt, F) HOTAIR-20nt, or G) PolyU-20nt. Scale bar, 10 μm. The corresponding normalized line-scan intensity profiles are shown.

    Article Snippet: PolyU and purified HOTAIR 1-360 were 3′-end labeled with Cy5 or Cy3 using T4 RNA ligase and Cy5- or Cy3-labeled cytidine-5′-phosphate-3′-(6-aminohexyl)phosphate (triethylammonium salt) purchased from Jena Bioscience.

    Techniques: Fluorescence, Microscopy, Labeling