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BioFire Defense blood culture identification 2 panel (bcid2
Blood Culture Identification 2 Panel (Bcid2, supplied by BioFire Defense, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2/bcid2+panel/nct05979545-2-10-9
Average 90 stars, based on 1 article reviews
blood culture identification 2 panel (bcid2 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Diagnostic Assay:

Article Title: Effectiveness of the BioFire FilmArray for the rapid detection of bloodstream infection in haematological patients with febrile neutropenia (the ONFIRE study): study protocol of a prospective, multicentre observational study at three reference university hospitals in Spain.
Article Snippet: Primary objective To assess whether the molecular technique BioFire FilmArray BCID2 panel obtains higher sensitivity and specificity than conventional blood cultures for the microbiological diagnosis of BSI in haematological patients with febrile neutropenia. .. Secondary objective To assess whether the increase in diagnostic performance of the BioFire FilmArray BCID2 panel is more significant in the subgroup of patients with recent (<48 hour) or concomitant use of systemic antibiotics than in those undergoing conventional blood cultures. ..

other:

Article Title: EaRly impAct theraPy With Ceftazidime-avibactam Via rapID Diagnostics Versus Standard of Care Antibiotics and Diagnostics in Patients With Bloodstream Infection, Hospital-acquired Pneumonia or Ventilator-associated Pneumonia Due to Pseudomonas Aeruginosa or Carbapenemase Producing Enterobacterales (RAPID)
Article Snippet: Patients randomised to the intervention arm, will have the BioFire Blood Culture Identification 2 Panel (BCID2) used for positive blood cultures and/or the BioFire FilmArray Pneumonia or Pneumonia plus Panel for respiratory tract specimens if having hospital-acquired pneumonia or ventilator-associated pneumonia.

Article Title: Observational quality control study: insourcing multi-PCR-impact on the use of anti-infectives for patients with pleocytosis.
Article Snippet: In addition to the Biofire®ME-Panel, other multiPCR panels have been invented and made commercially available.

Article Title: Observational quality control study: insourcing multi-PCR-impact on the use of anti-infectives for patients with pleocytosis.
Article Snippet: As especially a positive PCR for Enteroviridae is both important for the patient and - yielding a dischargesignal - for our department, the implementation of the Biofire®-ME PCR has led to a regular use in patients who display > 10 leukocytesμ l and to a restricted use for those between 5 and 10 leukocytes/μ l. As a result of our study, we implemented the parameters in a similar way as other hospitals [15].

Article Title: An Atypical Case of Infectious Myositis in a Young Woman on Immunosuppressive Therapy
Article Snippet: Repeat blood cultures remained negative, and additional testing, including a BioFire panel, Fungitell assay, cytomegalovirus, and Pneumocystis jirovecii , were all negative.

Article Title: Surveillance of Respiratory Pathogens Among Rapid Diagnostic Test-Negative Acute Respiratory Infection Patients in Myanmar in 2023, with a Focus on Rhinovirus and Enterovirus Genotyping.
Article Snippet: Third, the BioFire® multiplex PCR assay used in this study does not detect all respiratory pathogens, including certain bacterial and fungal agents.




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Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) <t>Representative</t> <t>Fura-2</t> F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) <t>Representative</t> <t>Fura-2</t> F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) <t>Representative</t> <t>Fura-2</t> F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) <t>Representative</t> <t>Fura-2</t> F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) <t>Representative</t> <t>Fura-2</t> F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) <t>Representative</t> <t>Fura-2</t> F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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Image Search Results


Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) Representative Fura-2 F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Journal: Bioactive Materials

Article Title: Reprogramming macrophage mechanosensation via TRPV4 modulating mechano-immunotherapy controls fibrotic encapsulation of biomaterial implants

doi: 10.1016/j.bioactmat.2026.06.020

Figure Lengend Snippet: Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) Representative Fura-2 F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Changes in intracellular calcium were measured using a ratiometric calcium indicator dye Fura-2 AM (ThermoFisher.

Techniques: Inhibition, Staining, Imaging

RN-1734-mediated modulation of TPRV4 downstream signalling following LPS stimulation. A-C) Dose-dependent ELISA quantification of A) TNF-α, B) IL-6 and C) MCP-1 cytokine secretion from macrophage cultures following 24 h LPS stimulation (n = 3), D) Representative western blots of macrophage lysates after 24 h post LPS stimulation for iNOS, ARG-1, p65, and α-tubulin. E-G) Protein quantification of western blots for iNOS, ARG-1, and p65 normalised to α-tubulin (n = 3). H) Schematic of GSK1016790A (GSK) mechanism: selective agonism of TRPV facilitating increased intracellular calcium influx. I) Representative Fura-2 imaging of macrophage cultures treated with GSK; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; scale bar = 30 μm. J-K) ELISA quantification of TNF-α and IL-6 secretion from macrophage cultures following 24 h of GSK stimulation (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Journal: Bioactive Materials

Article Title: Reprogramming macrophage mechanosensation via TRPV4 modulating mechano-immunotherapy controls fibrotic encapsulation of biomaterial implants

doi: 10.1016/j.bioactmat.2026.06.020

Figure Lengend Snippet: RN-1734-mediated modulation of TPRV4 downstream signalling following LPS stimulation. A-C) Dose-dependent ELISA quantification of A) TNF-α, B) IL-6 and C) MCP-1 cytokine secretion from macrophage cultures following 24 h LPS stimulation (n = 3), D) Representative western blots of macrophage lysates after 24 h post LPS stimulation for iNOS, ARG-1, p65, and α-tubulin. E-G) Protein quantification of western blots for iNOS, ARG-1, and p65 normalised to α-tubulin (n = 3). H) Schematic of GSK1016790A (GSK) mechanism: selective agonism of TRPV facilitating increased intracellular calcium influx. I) Representative Fura-2 imaging of macrophage cultures treated with GSK; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; scale bar = 30 μm. J-K) ELISA quantification of TNF-α and IL-6 secretion from macrophage cultures following 24 h of GSK stimulation (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Changes in intracellular calcium were measured using a ratiometric calcium indicator dye Fura-2 AM (ThermoFisher.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Imaging

Stiffness-dependent modulation of macrophage TPRV4 activity and signalling. A) Fabrication of 10% (stiff) and 5% (soft) GelMA hydrogels (scale bar: 2.5 mm) validated by compression test, B) Compression curves (inset: linear region of the curve). C) J774. a2 macrophage viability on GelMa hydrogels measured by alamarBlue and complementary (n = 3) D) brightfield imaging of macrophage morphology 24 h post-seeding; scale bar = 100 μm. E) Fura-2 (F340/380 ratio) calcium imaging traces of macrophage cultures over 60 min and F) quantification of changes from peak ratio relative to baseline (t = 0) (n > 50 per dish, 3 dishes), with G) representative culture images at t = 40mins; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; scale bar = 100 μm. H–J) ELISA of TNF-α, IL-6, and MCP-1 after 24 h on GelMA (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Journal: Bioactive Materials

Article Title: Reprogramming macrophage mechanosensation via TRPV4 modulating mechano-immunotherapy controls fibrotic encapsulation of biomaterial implants

doi: 10.1016/j.bioactmat.2026.06.020

Figure Lengend Snippet: Stiffness-dependent modulation of macrophage TPRV4 activity and signalling. A) Fabrication of 10% (stiff) and 5% (soft) GelMA hydrogels (scale bar: 2.5 mm) validated by compression test, B) Compression curves (inset: linear region of the curve). C) J774. a2 macrophage viability on GelMa hydrogels measured by alamarBlue and complementary (n = 3) D) brightfield imaging of macrophage morphology 24 h post-seeding; scale bar = 100 μm. E) Fura-2 (F340/380 ratio) calcium imaging traces of macrophage cultures over 60 min and F) quantification of changes from peak ratio relative to baseline (t = 0) (n > 50 per dish, 3 dishes), with G) representative culture images at t = 40mins; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; scale bar = 100 μm. H–J) ELISA of TNF-α, IL-6, and MCP-1 after 24 h on GelMA (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Changes in intracellular calcium were measured using a ratiometric calcium indicator dye Fura-2 AM (ThermoFisher.

Techniques: Activity Assay, Imaging, Enzyme-linked Immunosorbent Assay