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ActiGraph llc actiwatch 2
Actiwatch 2, supplied by ActiGraph llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2/actiwatch/nct04545606-27-0-2
Average 90 stars, based on 1 article reviews
actiwatch 2 - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Sleep following A Stroke: Multimodal Evaluation of Sleep Health & Disruptions, and Impact on Recovery during Acute Inpatient Rehabilitation
Article Snippet: Age (age<65 vs. age≥65) and PSQI (PSQI<5 vs. PSQI≥5) subgroups had significantly different actigraphy-based sleep metrics (i.e., reduced discharge sleep fragmentation and higher discharge daytime activity from Actiwatch, and shorter discharge sleep duration [Actiwatch and ActGraph] for the younger age group; shorter discharge sleep latency [ActiGraph] and increased discharge sleep fragmentation [Actiwatch] for the better perceived sleep quality group).

Article Title: Feasibility and acceptability of wrist-worn actigraphy to measure frailty in homebound older adults
Article Snippet: Actigraph data show that 11 patient participants wore the Actiwatch 100% of the time without removing it.

Article Title: Sleep following A Stroke: Multimodal Evaluation of Sleep Health & Disruptions, and Impact on Recovery during Acute Inpatient Rehabilitation
Article Snippet: Individual models were developed for each of the sleep health measures, namely the KSL, sleep duration (TST), and nighttime activity (Total AC) ( for both Actiwatch and ActiGraph ).

Article Title: Does accelerometer-measured physical activity and sedentary time differ between manual, in-office, hybrid and remote workers?
Article Snippet: Physical activity was measured over seven consecutive days and nights using triaxial ActiGraph wActiSleep- BT accelerometers and the newer wGT3X- BT accelerometers (ActiGraph, Pensacola, Florida, USA), replacing the broken devices.

Activity Assay:

Article Title: Sleep Following a Stroke: Multimodal Evaluation of Sleep Health and Disruptions and Impact on Recovery During Acute Inpatient Rehabilitation.
Article Snippet: Background.. Our understanding of sleep during early stroke care and its impact on rehabilitation outcomes remains limited.. The objectives of this work were to (1) evaluate multidimensional sleep health and disruptions during acute inpatient rehabilitation for individuals with stroke, and (2) explore the relationship between sleep health/disruptions and functional recovery.

Article Title: Targeting Insomnia in School Aged Children With Autism Spectrum Disorder
Article Snippet: .. Measure Description: Actigraph (Actiwatch 2â, Philips Respironics) measures light and gross motor activity and daily diary report. ..



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Thermo Fisher ratiometric calcium indicator dye fura 2 am
Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) <t>Representative</t> <t>Fura-2</t> F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
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Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) Representative Fura-2 F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Journal: Bioactive Materials

Article Title: Reprogramming macrophage mechanosensation via TRPV4 modulating mechano-immunotherapy controls fibrotic encapsulation of biomaterial implants

doi: 10.1016/j.bioactmat.2026.06.020

Figure Lengend Snippet: Characterization of RN-1734 effects on J774a macrophages and LPS-induced calcium flux. A) Schematic of RN-1734 mechanism: selective TRPV4 inhibition reduce intracellular calcium influx. B) Brightfield images showing macrophage morphology after RN-1734 treatment (80 μM); Scale bar = 50 μm. C) Dose-dependent effects of RN-1734 on macrophage attachment after 1 h (n = 4), D) Live/dead staining at 24 h (n = 3), E) proliferation at day 1 and day 3 measured by alamarBlue (n = 3). F) Schematic of live-cell calcium imaging under LPS stimulation. G) Representative Fura-2 F 34 0/F380 ratio traces over 6 min. H) Quantification of F340/F380 ratio changes relative to respective baseline of each cell, 3 dishes (n > 76 cells per dish) I) Representative Fura-2 images of J774a cells pre- and post-LPS stimulation; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; Scale bar = 100 μm ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Changes in intracellular calcium were measured using a ratiometric calcium indicator dye Fura-2 AM (ThermoFisher.

Techniques: Inhibition, Staining, Imaging

RN-1734-mediated modulation of TPRV4 downstream signalling following LPS stimulation. A-C) Dose-dependent ELISA quantification of A) TNF-α, B) IL-6 and C) MCP-1 cytokine secretion from macrophage cultures following 24 h LPS stimulation (n = 3), D) Representative western blots of macrophage lysates after 24 h post LPS stimulation for iNOS, ARG-1, p65, and α-tubulin. E-G) Protein quantification of western blots for iNOS, ARG-1, and p65 normalised to α-tubulin (n = 3). H) Schematic of GSK1016790A (GSK) mechanism: selective agonism of TRPV facilitating increased intracellular calcium influx. I) Representative Fura-2 imaging of macrophage cultures treated with GSK; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; scale bar = 30 μm. J-K) ELISA quantification of TNF-α and IL-6 secretion from macrophage cultures following 24 h of GSK stimulation (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Journal: Bioactive Materials

Article Title: Reprogramming macrophage mechanosensation via TRPV4 modulating mechano-immunotherapy controls fibrotic encapsulation of biomaterial implants

doi: 10.1016/j.bioactmat.2026.06.020

Figure Lengend Snippet: RN-1734-mediated modulation of TPRV4 downstream signalling following LPS stimulation. A-C) Dose-dependent ELISA quantification of A) TNF-α, B) IL-6 and C) MCP-1 cytokine secretion from macrophage cultures following 24 h LPS stimulation (n = 3), D) Representative western blots of macrophage lysates after 24 h post LPS stimulation for iNOS, ARG-1, p65, and α-tubulin. E-G) Protein quantification of western blots for iNOS, ARG-1, and p65 normalised to α-tubulin (n = 3). H) Schematic of GSK1016790A (GSK) mechanism: selective agonism of TRPV facilitating increased intracellular calcium influx. I) Representative Fura-2 imaging of macrophage cultures treated with GSK; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; scale bar = 30 μm. J-K) ELISA quantification of TNF-α and IL-6 secretion from macrophage cultures following 24 h of GSK stimulation (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Changes in intracellular calcium were measured using a ratiometric calcium indicator dye Fura-2 AM (ThermoFisher.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Imaging

Stiffness-dependent modulation of macrophage TPRV4 activity and signalling. A) Fabrication of 10% (stiff) and 5% (soft) GelMA hydrogels (scale bar: 2.5 mm) validated by compression test, B) Compression curves (inset: linear region of the curve). C) J774. a2 macrophage viability on GelMa hydrogels measured by alamarBlue and complementary (n = 3) D) brightfield imaging of macrophage morphology 24 h post-seeding; scale bar = 100 μm. E) Fura-2 (F340/380 ratio) calcium imaging traces of macrophage cultures over 60 min and F) quantification of changes from peak ratio relative to baseline (t = 0) (n > 50 per dish, 3 dishes), with G) representative culture images at t = 40mins; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; scale bar = 100 μm. H–J) ELISA of TNF-α, IL-6, and MCP-1 after 24 h on GelMA (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Journal: Bioactive Materials

Article Title: Reprogramming macrophage mechanosensation via TRPV4 modulating mechano-immunotherapy controls fibrotic encapsulation of biomaterial implants

doi: 10.1016/j.bioactmat.2026.06.020

Figure Lengend Snippet: Stiffness-dependent modulation of macrophage TPRV4 activity and signalling. A) Fabrication of 10% (stiff) and 5% (soft) GelMA hydrogels (scale bar: 2.5 mm) validated by compression test, B) Compression curves (inset: linear region of the curve). C) J774. a2 macrophage viability on GelMa hydrogels measured by alamarBlue and complementary (n = 3) D) brightfield imaging of macrophage morphology 24 h post-seeding; scale bar = 100 μm. E) Fura-2 (F340/380 ratio) calcium imaging traces of macrophage cultures over 60 min and F) quantification of changes from peak ratio relative to baseline (t = 0) (n > 50 per dish, 3 dishes), with G) representative culture images at t = 40mins; Color scale: red = high [Ca 2+ ], yellow/green = intermediate, blue/purple = low [Ca 2+ ]; scale bar = 100 μm. H–J) ELISA of TNF-α, IL-6, and MCP-1 after 24 h on GelMA (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Changes in intracellular calcium were measured using a ratiometric calcium indicator dye Fura-2 AM (ThermoFisher.

Techniques: Activity Assay, Imaging, Enzyme-linked Immunosorbent Assay