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Verlag GmbH 2-d clean-up
2 D Clean Up, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2-d+clean-up/2+d+clean+up/pm19086099-66-0-20
Average 90 stars, based on 1 article reviews
2-d clean-up - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Compatibility of toluidine blue with laser microdissection and saturation labeling DIGE.
Article Snippet: Tissue fixation and staining protocols for laser microdissection are frequently not fully compatible with subsequent proteomic analysis.. We compared the effect of three common histological stains (toluidine blue (TB), hemotoxylin, and hematoxylin and eosin (HE)) on tissue visualization, protein recovery, the saturation labeling reaction, and 2-D electrophoresis.. TB provided the best visualization of colorectal tumor tissue during laser microdissection (LMD) and had a comparable effect on protein recovery and the saturation labeling reaction with hematoxylin, provided a modified 2-D clean-up protocol was used.



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