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dulbecco s phosphate buffered saline without ca 2 and mg 2  (Thermo Fisher)


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    Thermo Fisher dulbecco s phosphate buffered saline without ca 2 and mg 2
    Propagation of calcium signal within microglia after ATP stimulation (A) Baseline GCaMP8s expression. (B) Regions of interest (ROIs): one somatic (ROI 1) and two distal regions (ROI <t>2</t> <t>and</t> 3) were chosen. (C) Snapshots showing propagation of the fluorescence signal within the cell following ATP stimulation. (D) Normalized fluorescence traces (ΔF/F0, F0 = mean fluorescence intensity over 10 s prior to stimuli) recorded from ROIs in B. The period shaded in green indicates when ATP was present in the recording chamber. ROI 3 (most distal) exhibits spontaneous activity prior to stimulation, indicated by asterisks. Dashed vertical lines indicate time points (t0-t3) corresponding to images in C.
    Dulbecco S Phosphate Buffered Saline Without Ca 2 And Mg 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2+buffer/pmc13068558-20-0-14?v=Thermo+Fisher
    Average 96 stars, based on 1 article reviews
    dulbecco s phosphate buffered saline without ca 2 and mg 2 - by Bioz Stars, 2026-08
    96/100 stars

    Images

    1) Product Images from "Protocol for differentiation and efficient AAV-mediated gene delivery to hiPSC-derived microglia for functional studies"

    Article Title: Protocol for differentiation and efficient AAV-mediated gene delivery to hiPSC-derived microglia for functional studies

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2026.104455

    Propagation of calcium signal within microglia after ATP stimulation (A) Baseline GCaMP8s expression. (B) Regions of interest (ROIs): one somatic (ROI 1) and two distal regions (ROI 2 and 3) were chosen. (C) Snapshots showing propagation of the fluorescence signal within the cell following ATP stimulation. (D) Normalized fluorescence traces (ΔF/F0, F0 = mean fluorescence intensity over 10 s prior to stimuli) recorded from ROIs in B. The period shaded in green indicates when ATP was present in the recording chamber. ROI 3 (most distal) exhibits spontaneous activity prior to stimulation, indicated by asterisks. Dashed vertical lines indicate time points (t0-t3) corresponding to images in C.
    Figure Legend Snippet: Propagation of calcium signal within microglia after ATP stimulation (A) Baseline GCaMP8s expression. (B) Regions of interest (ROIs): one somatic (ROI 1) and two distal regions (ROI 2 and 3) were chosen. (C) Snapshots showing propagation of the fluorescence signal within the cell following ATP stimulation. (D) Normalized fluorescence traces (ΔF/F0, F0 = mean fluorescence intensity over 10 s prior to stimuli) recorded from ROIs in B. The period shaded in green indicates when ATP was present in the recording chamber. ROI 3 (most distal) exhibits spontaneous activity prior to stimulation, indicated by asterisks. Dashed vertical lines indicate time points (t0-t3) corresponding to images in C.

    Techniques Used: Expressing, Fluorescence, Activity Assay


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Saline, Plasmid Preparation, Expressing, Software, Hood, Sterility, Electron Microscopy, Inverted Microscopy, Flow Cytometry, Microscopy, Cell Culture, Fluorescence, Imaging, Dispersion



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    Image Search Results


    Propagation of calcium signal within microglia after ATP stimulation (A) Baseline GCaMP8s expression. (B) Regions of interest (ROIs): one somatic (ROI 1) and two distal regions (ROI 2 and 3) were chosen. (C) Snapshots showing propagation of the fluorescence signal within the cell following ATP stimulation. (D) Normalized fluorescence traces (ΔF/F0, F0 = mean fluorescence intensity over 10 s prior to stimuli) recorded from ROIs in B. The period shaded in green indicates when ATP was present in the recording chamber. ROI 3 (most distal) exhibits spontaneous activity prior to stimulation, indicated by asterisks. Dashed vertical lines indicate time points (t0-t3) corresponding to images in C.

    Journal: STAR Protocols

    Article Title: Protocol for differentiation and efficient AAV-mediated gene delivery to hiPSC-derived microglia for functional studies

    doi: 10.1016/j.xpro.2026.104455

    Figure Lengend Snippet: Propagation of calcium signal within microglia after ATP stimulation (A) Baseline GCaMP8s expression. (B) Regions of interest (ROIs): one somatic (ROI 1) and two distal regions (ROI 2 and 3) were chosen. (C) Snapshots showing propagation of the fluorescence signal within the cell following ATP stimulation. (D) Normalized fluorescence traces (ΔF/F0, F0 = mean fluorescence intensity over 10 s prior to stimuli) recorded from ROIs in B. The period shaded in green indicates when ATP was present in the recording chamber. ROI 3 (most distal) exhibits spontaneous activity prior to stimulation, indicated by asterisks. Dashed vertical lines indicate time points (t0-t3) corresponding to images in C.

    Article Snippet: Dulbecco’s phosphate buffered saline without Ca 2+ and Mg 2+ , DPBS (−/−) , Thermo Fisher Scientific , 14190–086.

    Techniques: Expressing, Fluorescence, Activity Assay

    Journal: STAR Protocols

    Article Title: Protocol for differentiation and efficient AAV-mediated gene delivery to hiPSC-derived microglia for functional studies

    doi: 10.1016/j.xpro.2026.104455

    Figure Lengend Snippet:

    Article Snippet: Dulbecco’s phosphate buffered saline without Ca 2+ and Mg 2+ , DPBS (−/−) , Thermo Fisher Scientific , 14190–086.

    Techniques: Virus, Recombinant, Saline, Plasmid Preparation, Expressing, Software, Hood, Sterility, Electron Microscopy, Inverted Microscopy, Flow Cytometry, Microscopy, Cell Culture, Fluorescence, Imaging, Dispersion