1xte solution (Integrated DNA Technologies)
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Integrated DNA Technologies
1xte solution
1xte Solution, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 97/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1xte+buffer+solution/1X+TE+solution%2C+ph+8%2E0/pmc08864937-54-0-13
Average 97 stars, based on 98 article reviews
1xte Solution, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 97/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1xte+buffer+solution/1X+TE+solution%2C+ph+8%2E0/pmc08864937-54-0-13
Average 97 stars, based on 98 article reviews
1xte solution - by Bioz Stars,
2026-09
97/100 stars
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Hybridization:Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: Nucleic acid and library quality control TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes 2.3. .. Library preparation, hybridization, and sequencing Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes .. Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Sequencing:Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: Nucleic acid and library quality control TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes 2.3. .. Library preparation, hybridization, and sequencing Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Transferring:Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: Nucleic acid and library quality control TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes 2.3. .. Library preparation, hybridization, and sequencing Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes .. Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Stripping Membranes:Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: Nucleic acid and library quality control TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes 2.3. .. Library preparation, hybridization, and sequencing Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes .. Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Polymerase Chain Reaction:Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: Nucleic acid and library quality control TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes 2.3. .. Library preparation, hybridization, and sequencing Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes .. Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Amplification:Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: Nucleic acid and library quality control TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes 2.3. .. Library preparation, hybridization, and sequencing Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes .. Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Ligation:Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: Nucleic acid and library quality control TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes 2.3. .. Library preparation, hybridization, and sequencing Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes .. Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Software:Article Title: Detection of microsatellite instability biomarkers via next-generation sequencing Article Snippet: Nucleic acid and library quality control TapeStation 2200 (Agilent Technologies) with: Strip tubes Strip caps Loading tips Genomic DNA ScreenTape (4°C) Genomic DNA Reagents (4°C) D1000 ScreenTape (4°C) D1000 Reagents (4°C) Nanodrop 2000 Spectrophotometer with: Blanking solution: Qiagen AE Buffer, Nuclease-free water, or Qiagen ATE Buffer Qubit 2.0 Fluorometer with: Qubit dsDNA High Sensitivity or Broad Range Assay Kit Qubit Standards Qubit Assay Tubes 2.3. .. Library preparation, hybridization, and sequencing Pipettes (0.5–10 uL, 2–20 uL, 20–200 uL, 200–1000 uL) and plastic pipette tips Centrifuge Thermomixer Vortexer Heatblock Magnet strip Thermal cycler Microcentrifuge tubes PCR strip tubes Ice bucket Ethanol: 200 proof, room temperature NaOH: 2N, −20°C AMPure XP beads, 4°C 2X KAPA HiFi HotStart ReadyMix (−20°C, included in kit) Nuclease-free water KAPA Library Amplification Primer Mix (−20°C, included in kit) KAPA End Repair & A-Tailing Buffer (−20°C, included in kit) KAPA End Repair & A-Tailing Enzyme (−20°C, included in kit) KAPA Ligation Buffer (−20°C, included in kit) KAPA DNA Ligase (−20°C, included in kit) IDT Single Index Duplex Adapters (−20°C) Cot-1 DNA: 1 ug/ul, −20°C xGen Universal Blockers – TS Mix (−20°C) xGen Hybridization and Wash Kit (−20°C) DynaBeads® M-270 Streptavidin Beads (4°C) xGen Library Amplification Primer – TS Mix (−20°C) IDTE pH 8.0 ( Article Title: Triplex digital PCR assays for the quantification of intact proviral HIV-1 DNA. Article Snippet: The development of an HIV-1 cure is hampered by the existence of a persistent (latent) reservoir that contains a small proportion of replication-competent intact proviruses which refuels viral replication upon treatment discontinuation.. Therefore, an accurate evaluation and quantification of these (intact) proviruses is essential to determine the efficacy of HIV-1 cure strategies which aim to eliminate this reservoir.. Here, we present two triplex digital PCR assays which resulted from a combination of two existing methods, the IPDA (a 2-colour digital PCR based method) and Q4PCR assays (4 colour qPCR method), and tested the functionality on a three-colour digital PCR platform. Multiplex Assay:Article Title: Triplex digital PCR assays for the quantification of intact proviral HIV-1 DNA. Article Snippet: The development of an HIV-1 cure is hampered by the existence of a persistent (latent) reservoir that contains a small proportion of replication-competent intact proviruses which refuels viral replication upon treatment discontinuation.. Therefore, an accurate evaluation and quantification of these (intact) proviruses is essential to determine the efficacy of HIV-1 cure strategies which aim to eliminate this reservoir.. Here, we present two triplex digital PCR assays which resulted from a combination of two existing methods, the IPDA (a 2-colour digital PCR based method) and Q4PCR assays (4 colour qPCR method), and tested the functionality on a three-colour digital PCR platform. Real-time Polymerase Chain Reaction:Article Title: Triplex digital PCR assays for the quantification of intact proviral HIV-1 DNA. Article Snippet: The development of an HIV-1 cure is hampered by the existence of a persistent (latent) reservoir that contains a small proportion of replication-competent intact proviruses which refuels viral replication upon treatment discontinuation.. Therefore, an accurate evaluation and quantification of these (intact) proviruses is essential to determine the efficacy of HIV-1 cure strategies which aim to eliminate this reservoir.. Here, we present two triplex digital PCR assays which resulted from a combination of two existing methods, the IPDA (a 2-colour digital PCR based method) and Q4PCR assays (4 colour qPCR method), and tested the functionality on a three-colour digital PCR platform. |