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1x pbs  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 1x pbs
    1x Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+zymogram+developing+buffer/Phosphate-buffered+saline+(PBS%2C+1X)%2C+sterile-filtered/pmc09382340-442-12-21
    Average 99 stars, based on 1 article reviews
    1x pbs - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Saline:

    Article Title: Multiplexed Detection of Membranous Nephropathy Antigens by Multi-Reaction Monitoring Mass Spectrometry
    Article Snippet: .. Frozen kidney biopsy tissue remnants were washed in phosphate buffered saline, followed by bead-beating in 400 μl Pierce IP lysis buffer (Thermo Scientific #87787) containing protease and phosphatase inhibitors (Halt protease/phosphatase inhibitor at 1:100 dilution, Thermo Scientific #87786) to produce protein extracts. .. Protein extracts were immunoprecipitated with protein A/G beads (Pierce #88803) in the presence of 50 ng stable isotope-labeled universal monoclonal antibody (SILuMab K1;Sigma-Aldrich #MSQC6), and 50 ng SILuMab K4 (Sigma-Aldrich #MSQC7).

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: To evaluate changes in secondary protein structure, CD spectra were acquired using a circular dichroism spectropolarimeter (J-815; Jasco, Japan). .. Gel and GG were each prepared at a concentration of 0.1 mg mL −1 in 1X phosphate-buffered saline (PBS, pH 7.4, Thermo Fisher Scientific). ..

    Article Title: Early nasal and lung transcriptomic profiles reveal pathways associated with divergent clinical outcomes following H7N1 high pathogenicity avian influenza virus infection
    Article Snippet: Whole blood samples were preserved in TRIzol Reagent (Invitrogen). .. Swabs were placed in 1 ml of sterile phosphate-buffered saline (PBS) containing 1% penicillin-streptomycin (Thermo Fisher Scientific) and nystatin (Sigma-Aldrich). ..

    Article Title: Obtention of conditional media from Wharton’s gelatin mesenchymal stromal cells in a dynamic culture system
    Article Snippet: .. Luego, los cordones se transportaron al laboratorio, debidamente refrigerados e inmersos en una solución salina tampón (Phosphate Buffered Saline, PBS) (PBS 1X, GIBCO), con suplemento al 1 % de antibiótico y antimicótico (Sigma Aldrich Inc.). ..

    Article Title: Collagen II hydrogel-mediated sustained delivery of lacosamide attenuates cartilage degeneration and pain in osteoarthritis
    Article Snippet: .. Their initial weight (W 0 ), was recorded, and they were then immersed in 1x phosphate-buffered saline (PBS, Gibco) at a constant temperature of 37 °C. ..

    Article Title: Polyplex of peptide-mannan and RNA for intranasal delivery of TGF-β siRNA in treatment of pulmonary fibrosis
    Article Snippet: GAPDH reverse and forward primers were purchased from Integrated DNA Technologies, Inc (Coralville, IA). .. Zeba Spin Desalting Columns (7k MOCO), RiboGreen RNA Assay Kit, PowerUp SYBR Green Master Mix, High-Capacity cDNA Reverse Transcription Kit, DMEM/F-12, CHCA MALDI Matrix, and fetal bovine serum (FBS), Alexa Fluor 647 Dye (AF647), phosphate-buffered saline (PBS, pH = 7.4) were purchased from ThermoFisher Scientific (Waltham, MA). .. TGF-β siRNA and Cy5-labeled TGF-β siRNA were purchased from Horizon discovery (Cambridge, UK).

    Article Title: Synergistic Induction of Caspase-8-Mediated Leukaemic Cell Death by Fisetin and Pinocembrin.
    Article Snippet: .. Jurkat cells were seeded at a density of 1 × 105 cells/mL and treated with fisetin at concentrations of 5, 10, 15 and 20 μM for 48 h. After treatment, cells were collected, washed twice with cold 1X phosphate-buffered saline (PBS), and lysed in RIPA buffer (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1X protease inhibitor cocktail (Thermo Fisher Scientific, Rockford, IL, USA) on ice for 15 min. .. The total protein concentrations were quantified using a bicinchoninic acid (BCA) protein assay (Thermo Scientific, Waltham, MA, USA).

    Lysis:

    Article Title: Multiplexed Detection of Membranous Nephropathy Antigens by Multi-Reaction Monitoring Mass Spectrometry
    Article Snippet: .. Frozen kidney biopsy tissue remnants were washed in phosphate buffered saline, followed by bead-beating in 400 μl Pierce IP lysis buffer (Thermo Scientific #87787) containing protease and phosphatase inhibitors (Halt protease/phosphatase inhibitor at 1:100 dilution, Thermo Scientific #87786) to produce protein extracts. .. Protein extracts were immunoprecipitated with protein A/G beads (Pierce #88803) in the presence of 50 ng stable isotope-labeled universal monoclonal antibody (SILuMab K1;Sigma-Aldrich #MSQC6), and 50 ng SILuMab K4 (Sigma-Aldrich #MSQC7).

    Concentration Assay:

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: To evaluate changes in secondary protein structure, CD spectra were acquired using a circular dichroism spectropolarimeter (J-815; Jasco, Japan). .. Gel and GG were each prepared at a concentration of 0.1 mg mL −1 in 1X phosphate-buffered saline (PBS, pH 7.4, Thermo Fisher Scientific). ..

    Sterility:

    Article Title: Early nasal and lung transcriptomic profiles reveal pathways associated with divergent clinical outcomes following H7N1 high pathogenicity avian influenza virus infection
    Article Snippet: Whole blood samples were preserved in TRIzol Reagent (Invitrogen). .. Swabs were placed in 1 ml of sterile phosphate-buffered saline (PBS) containing 1% penicillin-streptomycin (Thermo Fisher Scientific) and nystatin (Sigma-Aldrich). ..

    Blocking Assay:

    Article Title: Hyperlipidemia induces hippocampal inflammation and loss of vascularity and can be rescued by silencing RIPK1.
    Article Snippet: Serial crosssections Ten μm-thick sections were sectioned with a Thermo Cryostat (Scientific #HM550). .. Slides were rinsed 3x with PBS 1x and permeabilized with PBS-T 5% (5% Triton-X in PBS) solution 3x for 5 min and block for 1.5 h in a humid slide chamber with blocking solution (10% normal donkey serum and 5% fish gelatin in PBS-T 5%), followed by primary antibodies in blocking solution for 24 hr at 4°C overnight, and finally with Alexa Fluor-conjugated secondary antibodies (1:500, Invitrogen) in blocking solution for 2h at room temperature in a humid slide chamber. .. Slides were mounted with aqueous mounting medium (Dako #s3023).

    SYBR Green Assay:

    Article Title: Polyplex of peptide-mannan and RNA for intranasal delivery of TGF-β siRNA in treatment of pulmonary fibrosis
    Article Snippet: GAPDH reverse and forward primers were purchased from Integrated DNA Technologies, Inc (Coralville, IA). .. Zeba Spin Desalting Columns (7k MOCO), RiboGreen RNA Assay Kit, PowerUp SYBR Green Master Mix, High-Capacity cDNA Reverse Transcription Kit, DMEM/F-12, CHCA MALDI Matrix, and fetal bovine serum (FBS), Alexa Fluor 647 Dye (AF647), phosphate-buffered saline (PBS, pH = 7.4) were purchased from ThermoFisher Scientific (Waltham, MA). .. TGF-β siRNA and Cy5-labeled TGF-β siRNA were purchased from Horizon discovery (Cambridge, UK).

    Reverse Transcription:

    Article Title: Polyplex of peptide-mannan and RNA for intranasal delivery of TGF-β siRNA in treatment of pulmonary fibrosis
    Article Snippet: GAPDH reverse and forward primers were purchased from Integrated DNA Technologies, Inc (Coralville, IA). .. Zeba Spin Desalting Columns (7k MOCO), RiboGreen RNA Assay Kit, PowerUp SYBR Green Master Mix, High-Capacity cDNA Reverse Transcription Kit, DMEM/F-12, CHCA MALDI Matrix, and fetal bovine serum (FBS), Alexa Fluor 647 Dye (AF647), phosphate-buffered saline (PBS, pH = 7.4) were purchased from ThermoFisher Scientific (Waltham, MA). .. TGF-β siRNA and Cy5-labeled TGF-β siRNA were purchased from Horizon discovery (Cambridge, UK).

    Protease Inhibitor:

    Article Title: Synergistic Induction of Caspase-8-Mediated Leukaemic Cell Death by Fisetin and Pinocembrin.
    Article Snippet: .. Jurkat cells were seeded at a density of 1 × 105 cells/mL and treated with fisetin at concentrations of 5, 10, 15 and 20 μM for 48 h. After treatment, cells were collected, washed twice with cold 1X phosphate-buffered saline (PBS), and lysed in RIPA buffer (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1X protease inhibitor cocktail (Thermo Fisher Scientific, Rockford, IL, USA) on ice for 15 min. .. The total protein concentrations were quantified using a bicinchoninic acid (BCA) protein assay (Thermo Scientific, Waltham, MA, USA).



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