culture medium dpbs (Thermo Fisher)

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Thermo Fisher culture medium dpbs
Culture Medium Dpbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+zymogram+developing+buffer/Phosphate-buffered+saline+(DPBS%2C+1X)%2C+Dulbecco's+formula%2C+without+calcium%2C+without+magnesium/pmc03546910-78-26-32
Average 99 stars, based on 1 article reviews

culture medium dpbs - by Bioz Stars, 2026-11

99/100 stars

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Saline:

Article Title: Recombinant bone morphogenetic protein-2 attenuates colorectal cancer progression by orchestrating Hippo pathway activation, Yes-associated protein inhibition, and epithelial-mesenchymal transition suppression.
Article Snippet: .. After medium replacement every 3 days, the cells were gently washed with Dulbecco's phosphate buffered saline (DPBS) (Gibco, Thermo Fisher Scientific) and fixed with 100% methanol at room temperature for 20 min. .. They were stained using 2% crystal violet (Sigma-Aldrich) for 5 min.

Article Title: Protocol for characterization of spatiotemporal network dynamics in cortical and hippocampal assembloids
Article Snippet: Neurobasal Medium , Gibco , 21-103-049. .. Phosphate-Buffered Saline (DPBS, 1×), Dulbecco’s formula, without calcium, without magnesium , ThermoScientific Chemicals , AAJ67802AP. .. EmbryoMax Primary Mouse Embryonic Fibroblasts (MEFs) , Sigma Aldrich , PMEM-CF.

Article Title: Energy substrate preference and metabolic flexibility in quiescent and oxytocin-stimulated human myometrial cells.
Article Snippet: .. Briefly, cells were transferred from 6-well plates to a round-bottom 96-well plate (Thermo Scientific) by washing with Dulbecco’s Phosphate Buffered Saline (DPBS; Gibco) and lifting cells with TrypLE Express Enzyme (Gibco). .. Data acquisition and analysis were performed using Lab Chart software (AD Instruments).

Sterility:

Article Title: ROS-NRF2 axis coordinates concentration-graded differentiation-to-PANoptosis switching in a phthalide combination therapy for acute myeloid leukemia.
Article Snippet: Background: In the context of multimodal therapeutic strategies for acute myeloid leukemia (AML), the coordinated regulation of cell death and differentiation pathways has emerged as a groundbreaking research

Membrane:

Bacteria:

other:

Article Title: Topical rewetting restores the autoimmune-damaged corneal stroma
Article Snippet: Following 3 washes with PBST, antibodies were detected using Cy2-, Cy3-or Cy5-conjugated secondary Fab fragment antibodies (Jackson Laboratories), and nuclei were stained with Hoechst 33342 (1:3000, Sigma-Aldrich).

Incubation:

Article Title: Cell-in-water: complementary optical photothermal infrared and Raman microscopy for submicron chemical imaging of hydrated cells.
Article Snippet: To induce the formation of LDs, cells were incubated with (10 μM or 100 μM) deuterated oleic acid (d34-OA; Sigma-Aldrich) complexed with fatty acid-free bovine serum albumin (BSA, Sigma-Aldrich) in a 6:1 M ratio for an additional 24 h. The saponification of d34-OA was performed using NaOH at 70 ◦C before conjugation with BSA. .. After incubation, cells were rinsed three times with Dulbecco's Phosphate Buffered Saline (DPBS, Gibco) to remove unbound lipids. .. HeLa (Cervical Cancer, female origin, MERCK, cat. no 93021013- 1VL) cells were cultured in high-glucose Dulbecco's Modified Eagle Medium (DMEM; Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum (FBS; Gibco) and 1% L-glutamine (Sigma-Aldrich) at 37 ◦C in a 5% CO2 atmosphere.

Article Title: SATB1 is a targetable modulator of JAK-STAT signaling and cytokines in human Treg and Tconv cells.
Article Snippet: In total, 1 ml cold ATAC buffer with 0.1% TWEEN-20 (Sigma-Aldrich) was used to stop the reaction. .. The cells were resuspended in 50 μl Transposase Mastermix: TD buffer (Illumina), 16.5 μl DPBS, no calcium, no magnesium (Thermo Fisher Scientific), 0.5 μl 1% Digitonin (Promega), 0.5 μl 10% Tween-20 (Sigma-Aldrich), and 100 nM Transposase (Illumina) and incubated for 30 min at 37 °C. .. The cleanup of the transposed fragments was performed with the Zymo DNA Clean and Concentrator-5 Kit (Zymo Research) according to the manufacturer’s instructions.

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