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tris buffered saline  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology tris buffered saline
    Tris Buffered Saline, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 10645 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+zymogram+developing+buffer/Tris+Buffered+Saline/pm40451821-61-6-17
    Average 98 stars, based on 10645 article reviews
    tris buffered saline - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Saline:

    Article Title: Drp1-Mediated Mitochondrial Fission Coordinates Mineral Precursor Transport and Mitochondrial Homeostasis During Osteogenesis
    Article Snippet: .. The next day, the membranes were washed with Tris-buffered saline with Tween, and the corresponding secondary antibodies (1: 3500, Santa Cruz Biotechnology, USA) were added for incubation at room temperature for 1 hour. .. Development was performed using an ECL chemiluminescence kit (Pierce Biotechnology, USA; catalog number: WP20005), and grayscale analysis was conducted using ImageJ software (ImageJ 1.5, NIH, USA) for grayscale analysis.

    Article Title: Sex-specific effects of chronic caffeine on impulsive choice and serotonergic markers in an ADHD rat model.
    Article Snippet: Aliquots corresponding to 40 μg protein were separated by SDS-PAGE (12% running gel with a 4% concentrating gel) and electro-transferred to nitrocellulose membranes (Amersham, São Paulo/Brazil). .. After blocking with Tris-buffered saline 0.1% Tween-20 (TBS-T) containing 3% BSA for 1 h at room temperature, the membranes were incubated overnight at 4o C with the following primary antibodies: mouse anti-5HT2A (1: 2,000; Santa Cruz, São Paulo/Brazil); rabbit anti-5HT1B (1: 2,000; Abcam, São Paulo/Brazil); rabbit anti-SERT (1: 4,000; Abcam, São Paulo/Brazil). .. The membranes were then washed with TBST, incubated with horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and developed with chemiluminescence ECL kit (Amersham, São Paulo/ Brazil).

    Article Title: Carnosol promotes myogenesis in livestock muscle stem cells through coordinated regulation of myostatin signaling and mitochondrial MSS51
    Article Snippet: Equal protein concentrations (50 μg) were separated by 8–10% sodium dodecyl–sulfate polyacrylamide gel electrophoresis (SDS–PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA, USA). .. Membranes were blocked in Tris-buffered saline (TBS) containing 0.1% Tween-20 and 3% skim milk or bovine serum albumin (BSA) for 1 h, then incubated overnight at 4 ◦C with primary antibodies against Ki67, PCNA, MYF5, MYOD, MYOG, MYH, MSS51, MSTN, SMAD2/3, pSMAD2/3, and β-actin (1:400 or 1:1000; Santa Cruz Biotechnology, Dallas, TX, USA, or Cell Signaling Technology, Danvers, MA, USA). .. After washing, membranes were incubated with HRP-conjugated goat antimouse or anti-rabbit antibodies (GeneTex, Irvine, CA, USA) at 25 ◦C for 1 h. Protein bands were visualized using the Dyne ECL Pico Plus detection kit (Dyne Bio, Seongnam, South Korea) and imaged with an Azure 300 chemiluminescence system (Azure Biosystems, Dublin, CA, USA).

    Article Title: Synthesis of dopamine-functionalized quantum graphene dot-dopamin-hyaluronic acid nanocomposite loaded with parthenolide and assessment of its impact on cancer cells: an in vitro model
    Article Snippet: PTL was purchased from AdipoGen Life Sciences (AG-CN2-0455). .. Dimethyl sulfoxide (DMSO), phosphate-buffered saline (PBS), and Tris-buffered saline (TBS) were sourced from Santa Cruz Biotechnology. ..

    Article Title: Loss of sirtuin SIRT1 promotes aggressiveness of colon adenocarcinoma by modulating voltage-gated sodium channel activity
    Article Snippet: The protein concentrations of the cell lysates were measured using Pierce Protein Assay Reagent (ThermoFisher scientific cat. 23,225) following the manufacturer’s instructions. .. Samples containing equal quantities (40 μg) of total protein were resolved in denaturing SDS–polyacrylamide 8–12%, then transferred to nitrocellulose membranes by means of the wet transfer technique for a period of 100 min at 90 V. The membranes were incubated in Tris-buffered saline plus Tween (TBST 0.001%) containing 5% skimmed milk for one hour at ambient temperature, then probed overnight at 4 °C with primary antibodies purchased from Cell signaling technology: SIRT1 (cat.2493S dilution 1:1000), E-cadherin (cat. 14472S dilution 1 :1000), ZO-1 (cat. 8193S dilution 1:1000), α-Smooth Muscle Actin Rabbit (cat. 19,245 dilution 1:750), β-Actin (cat. 4967S dilution 1:1000) and HSC-70 purchased from Santa Cruz (cat. SC-7298 dilution 1 :1000). .. After being washed three times with (TBST), the horseradish peroxidase conjugated AffiniPure Goat anti-Rabbit (Jackson Immunological Research cat. 111–035-003) or horseradish peroxidase-conjugated Goat anti-mouse (BIO-RAD cat. 170–6516) was employed at a dilution of (1:10.000) for hour at room temperature.

    Article Title: Protective Effect of Silymarin Against Paclitaxel‐Induced Cardiotoxicity
    Article Snippet: .. The membranes were blocked with 5% w/v bovine serum albumin for 90 min. Primary antibodies (IL‐1β [sc‐52,012], TNF‐α [sc‐52,746], Caspase‐3 [sc‐56,053], Bcl‐2 [sc‐7382], NF‐κB‐p65 [sc‐109], and beta‐actin [sc‐47,778], Santa Cruz Biotechnology, USA; P2X7R [11144–1‐AP], Proteintech Group, USA) were applied to the membranes and incubated at 4°C for approximately 15 h. Following a wash with Tris‐buffered saline containing Tween, the membranes were exposed to secondary antibodies (sc‐2004/sc‐2005, Santa Cruz Biotechnology, USA) conjugated with horseradish peroxidase (TP‐125‐HL, Thermo Fisher Scientific, USA) for 90 min. β‐actin (1:2000 dilution) was used as the loading control. .. Protein bands were visualized using enhanced Western ECL substrate (3405, Thermo Fisher Scientific, USA) and analyzed with software (Image Lab, Bio‐Rad, USA).

    Incubation:

    Article Title: Drp1-Mediated Mitochondrial Fission Coordinates Mineral Precursor Transport and Mitochondrial Homeostasis During Osteogenesis
    Article Snippet: .. The next day, the membranes were washed with Tris-buffered saline with Tween, and the corresponding secondary antibodies (1: 3500, Santa Cruz Biotechnology, USA) were added for incubation at room temperature for 1 hour. .. Development was performed using an ECL chemiluminescence kit (Pierce Biotechnology, USA; catalog number: WP20005), and grayscale analysis was conducted using ImageJ software (ImageJ 1.5, NIH, USA) for grayscale analysis.

    Article Title: Sex-specific effects of chronic caffeine on impulsive choice and serotonergic markers in an ADHD rat model.
    Article Snippet: Aliquots corresponding to 40 μg protein were separated by SDS-PAGE (12% running gel with a 4% concentrating gel) and electro-transferred to nitrocellulose membranes (Amersham, São Paulo/Brazil). .. After blocking with Tris-buffered saline 0.1% Tween-20 (TBS-T) containing 3% BSA for 1 h at room temperature, the membranes were incubated overnight at 4o C with the following primary antibodies: mouse anti-5HT2A (1: 2,000; Santa Cruz, São Paulo/Brazil); rabbit anti-5HT1B (1: 2,000; Abcam, São Paulo/Brazil); rabbit anti-SERT (1: 4,000; Abcam, São Paulo/Brazil). .. The membranes were then washed with TBST, incubated with horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and developed with chemiluminescence ECL kit (Amersham, São Paulo/ Brazil).

    Article Title: Carnosol promotes myogenesis in livestock muscle stem cells through coordinated regulation of myostatin signaling and mitochondrial MSS51
    Article Snippet: Equal protein concentrations (50 μg) were separated by 8–10% sodium dodecyl–sulfate polyacrylamide gel electrophoresis (SDS–PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA, USA). .. Membranes were blocked in Tris-buffered saline (TBS) containing 0.1% Tween-20 and 3% skim milk or bovine serum albumin (BSA) for 1 h, then incubated overnight at 4 ◦C with primary antibodies against Ki67, PCNA, MYF5, MYOD, MYOG, MYH, MSS51, MSTN, SMAD2/3, pSMAD2/3, and β-actin (1:400 or 1:1000; Santa Cruz Biotechnology, Dallas, TX, USA, or Cell Signaling Technology, Danvers, MA, USA). .. After washing, membranes were incubated with HRP-conjugated goat antimouse or anti-rabbit antibodies (GeneTex, Irvine, CA, USA) at 25 ◦C for 1 h. Protein bands were visualized using the Dyne ECL Pico Plus detection kit (Dyne Bio, Seongnam, South Korea) and imaged with an Azure 300 chemiluminescence system (Azure Biosystems, Dublin, CA, USA).

    Article Title: Loss of sirtuin SIRT1 promotes aggressiveness of colon adenocarcinoma by modulating voltage-gated sodium channel activity
    Article Snippet: The protein concentrations of the cell lysates were measured using Pierce Protein Assay Reagent (ThermoFisher scientific cat. 23,225) following the manufacturer’s instructions. .. Samples containing equal quantities (40 μg) of total protein were resolved in denaturing SDS–polyacrylamide 8–12%, then transferred to nitrocellulose membranes by means of the wet transfer technique for a period of 100 min at 90 V. The membranes were incubated in Tris-buffered saline plus Tween (TBST 0.001%) containing 5% skimmed milk for one hour at ambient temperature, then probed overnight at 4 °C with primary antibodies purchased from Cell signaling technology: SIRT1 (cat.2493S dilution 1:1000), E-cadherin (cat. 14472S dilution 1 :1000), ZO-1 (cat. 8193S dilution 1:1000), α-Smooth Muscle Actin Rabbit (cat. 19,245 dilution 1:750), β-Actin (cat. 4967S dilution 1:1000) and HSC-70 purchased from Santa Cruz (cat. SC-7298 dilution 1 :1000). .. After being washed three times with (TBST), the horseradish peroxidase conjugated AffiniPure Goat anti-Rabbit (Jackson Immunological Research cat. 111–035-003) or horseradish peroxidase-conjugated Goat anti-mouse (BIO-RAD cat. 170–6516) was employed at a dilution of (1:10.000) for hour at room temperature.

    Article Title: Protective Effect of Silymarin Against Paclitaxel‐Induced Cardiotoxicity
    Article Snippet: .. The membranes were blocked with 5% w/v bovine serum albumin for 90 min. Primary antibodies (IL‐1β [sc‐52,012], TNF‐α [sc‐52,746], Caspase‐3 [sc‐56,053], Bcl‐2 [sc‐7382], NF‐κB‐p65 [sc‐109], and beta‐actin [sc‐47,778], Santa Cruz Biotechnology, USA; P2X7R [11144–1‐AP], Proteintech Group, USA) were applied to the membranes and incubated at 4°C for approximately 15 h. Following a wash with Tris‐buffered saline containing Tween, the membranes were exposed to secondary antibodies (sc‐2004/sc‐2005, Santa Cruz Biotechnology, USA) conjugated with horseradish peroxidase (TP‐125‐HL, Thermo Fisher Scientific, USA) for 90 min. β‐actin (1:2000 dilution) was used as the loading control. .. Protein bands were visualized using enhanced Western ECL substrate (3405, Thermo Fisher Scientific, USA) and analyzed with software (Image Lab, Bio‐Rad, USA).

    Blocking Assay:

    Article Title: Sex-specific effects of chronic caffeine on impulsive choice and serotonergic markers in an ADHD rat model.
    Article Snippet: Aliquots corresponding to 40 μg protein were separated by SDS-PAGE (12% running gel with a 4% concentrating gel) and electro-transferred to nitrocellulose membranes (Amersham, São Paulo/Brazil). .. After blocking with Tris-buffered saline 0.1% Tween-20 (TBS-T) containing 3% BSA for 1 h at room temperature, the membranes were incubated overnight at 4o C with the following primary antibodies: mouse anti-5HT2A (1: 2,000; Santa Cruz, São Paulo/Brazil); rabbit anti-5HT1B (1: 2,000; Abcam, São Paulo/Brazil); rabbit anti-SERT (1: 4,000; Abcam, São Paulo/Brazil). .. The membranes were then washed with TBST, incubated with horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and developed with chemiluminescence ECL kit (Amersham, São Paulo/ Brazil).

    Transcranial Magnetic Stimulation:

    Article Title: Carnosol promotes myogenesis in livestock muscle stem cells through coordinated regulation of myostatin signaling and mitochondrial MSS51
    Article Snippet: Equal protein concentrations (50 μg) were separated by 8–10% sodium dodecyl–sulfate polyacrylamide gel electrophoresis (SDS–PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA, USA). .. Membranes were blocked in Tris-buffered saline (TBS) containing 0.1% Tween-20 and 3% skim milk or bovine serum albumin (BSA) for 1 h, then incubated overnight at 4 ◦C with primary antibodies against Ki67, PCNA, MYF5, MYOD, MYOG, MYH, MSS51, MSTN, SMAD2/3, pSMAD2/3, and β-actin (1:400 or 1:1000; Santa Cruz Biotechnology, Dallas, TX, USA, or Cell Signaling Technology, Danvers, MA, USA). .. After washing, membranes were incubated with HRP-conjugated goat antimouse or anti-rabbit antibodies (GeneTex, Irvine, CA, USA) at 25 ◦C for 1 h. Protein bands were visualized using the Dyne ECL Pico Plus detection kit (Dyne Bio, Seongnam, South Korea) and imaged with an Azure 300 chemiluminescence system (Azure Biosystems, Dublin, CA, USA).

    Article Title: Synthesis of dopamine-functionalized quantum graphene dot-dopamin-hyaluronic acid nanocomposite loaded with parthenolide and assessment of its impact on cancer cells: an in vitro model
    Article Snippet: PTL was purchased from AdipoGen Life Sciences (AG-CN2-0455). .. Dimethyl sulfoxide (DMSO), phosphate-buffered saline (PBS), and Tris-buffered saline (TBS) were sourced from Santa Cruz Biotechnology. ..

    Cell-Signaling:

    Article Title: Carnosol promotes myogenesis in livestock muscle stem cells through coordinated regulation of myostatin signaling and mitochondrial MSS51
    Article Snippet: Equal protein concentrations (50 μg) were separated by 8–10% sodium dodecyl–sulfate polyacrylamide gel electrophoresis (SDS–PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA, USA). .. Membranes were blocked in Tris-buffered saline (TBS) containing 0.1% Tween-20 and 3% skim milk or bovine serum albumin (BSA) for 1 h, then incubated overnight at 4 ◦C with primary antibodies against Ki67, PCNA, MYF5, MYOD, MYOG, MYH, MSS51, MSTN, SMAD2/3, pSMAD2/3, and β-actin (1:400 or 1:1000; Santa Cruz Biotechnology, Dallas, TX, USA, or Cell Signaling Technology, Danvers, MA, USA). .. After washing, membranes were incubated with HRP-conjugated goat antimouse or anti-rabbit antibodies (GeneTex, Irvine, CA, USA) at 25 ◦C for 1 h. Protein bands were visualized using the Dyne ECL Pico Plus detection kit (Dyne Bio, Seongnam, South Korea) and imaged with an Azure 300 chemiluminescence system (Azure Biosystems, Dublin, CA, USA).

    Article Title: Loss of sirtuin SIRT1 promotes aggressiveness of colon adenocarcinoma by modulating voltage-gated sodium channel activity
    Article Snippet: The protein concentrations of the cell lysates were measured using Pierce Protein Assay Reagent (ThermoFisher scientific cat. 23,225) following the manufacturer’s instructions. .. Samples containing equal quantities (40 μg) of total protein were resolved in denaturing SDS–polyacrylamide 8–12%, then transferred to nitrocellulose membranes by means of the wet transfer technique for a period of 100 min at 90 V. The membranes were incubated in Tris-buffered saline plus Tween (TBST 0.001%) containing 5% skimmed milk for one hour at ambient temperature, then probed overnight at 4 °C with primary antibodies purchased from Cell signaling technology: SIRT1 (cat.2493S dilution 1:1000), E-cadherin (cat. 14472S dilution 1 :1000), ZO-1 (cat. 8193S dilution 1:1000), α-Smooth Muscle Actin Rabbit (cat. 19,245 dilution 1:750), β-Actin (cat. 4967S dilution 1:1000) and HSC-70 purchased from Santa Cruz (cat. SC-7298 dilution 1 :1000). .. After being washed three times with (TBST), the horseradish peroxidase conjugated AffiniPure Goat anti-Rabbit (Jackson Immunological Research cat. 111–035-003) or horseradish peroxidase-conjugated Goat anti-mouse (BIO-RAD cat. 170–6516) was employed at a dilution of (1:10.000) for hour at room temperature.

    other:

    Article Title: Prenatal lipopolysaccharide exposure programs cardiac fibrosis via dysregulating of connexin 43 in offspring rats
    Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto 0.45 μm polyvinylidene fluoride (PVDF) membranes (Millipore.

    Control:

    Article Title: Protective Effect of Silymarin Against Paclitaxel‐Induced Cardiotoxicity
    Article Snippet: .. The membranes were blocked with 5% w/v bovine serum albumin for 90 min. Primary antibodies (IL‐1β [sc‐52,012], TNF‐α [sc‐52,746], Caspase‐3 [sc‐56,053], Bcl‐2 [sc‐7382], NF‐κB‐p65 [sc‐109], and beta‐actin [sc‐47,778], Santa Cruz Biotechnology, USA; P2X7R [11144–1‐AP], Proteintech Group, USA) were applied to the membranes and incubated at 4°C for approximately 15 h. Following a wash with Tris‐buffered saline containing Tween, the membranes were exposed to secondary antibodies (sc‐2004/sc‐2005, Santa Cruz Biotechnology, USA) conjugated with horseradish peroxidase (TP‐125‐HL, Thermo Fisher Scientific, USA) for 90 min. β‐actin (1:2000 dilution) was used as the loading control. .. Protein bands were visualized using enhanced Western ECL substrate (3405, Thermo Fisher Scientific, USA) and analyzed with software (Image Lab, Bio‐Rad, USA).



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