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human glass 12k cdna chip  (Johns Hopkins HealthCare)

 
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    Structured Review

    Johns Hopkins HealthCare human glass 12k cdna chip
    Expression of IL-6 in TSU-Pr1 cells. TSU-Pr1 cells stably transfected with pp32 anti-sense express higher levels of IL-6 message as compared to parental TSU-Pr1 cells and vector-only control by RT-PCR analysis, which validates the <t>cDNA</t> microarray analysis (see Figure 6).
    Human Glass 12k Cdna Chip, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/12k+cdna+microarray/human+proteome+microarray/pmc01602226-105-21-12
    Average 90 stars, based on 1 article reviews
    human glass 12k cdna chip - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "pp32 Reduction Induces Differentiation of TSU-Pr1 Cells"

    Article Title: pp32 Reduction Induces Differentiation of TSU-Pr1 Cells

    Journal:

    doi:

    Expression of IL-6 in TSU-Pr1 cells. TSU-Pr1 cells stably transfected with pp32 anti-sense express higher levels of IL-6 message as compared to parental TSU-Pr1 cells and vector-only control by RT-PCR analysis, which validates the cDNA microarray analysis (see Figure 6).
    Figure Legend Snippet: Expression of IL-6 in TSU-Pr1 cells. TSU-Pr1 cells stably transfected with pp32 anti-sense express higher levels of IL-6 message as compared to parental TSU-Pr1 cells and vector-only control by RT-PCR analysis, which validates the cDNA microarray analysis (see Figure 6).

    Techniques Used: Expressing, Stable Transfection, Transfection, Plasmid Preparation, Control, Reverse Transcription Polymerase Chain Reaction, Microarray

    Related Articles

    Microarray:

    Article Title: Chemical proteomics accelerates the target discovery of natural products.
    Article Snippet: More than half of the global novel drugs are directly or indirectly derived from natural products (NPs) because of their better selectivity towards proteins.. Traditional medicines perform multiple bioactivities through various NPs binding to drug targets, which highlights the opportunities of target discovery for drug development.. However, detecting the binding relationship between NPs and targets remains challenging.

    Article Title: Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization.
    Article Snippet: .. The HuProt microarray was obtained from the Johns Hopkins Medical Institutions Protein Microarray Core (CDI Laboratories, Inc).The array experiment was performed by Wayen Biotechnologies (Shanghai, China) according to the following procedure. ..

    Article Title: E17241 as a Novel ABCA1 (ATP-Binding Cassette Transporter A1) Upregulator Ameliorates Atherosclerosis in Mice
    Article Snippet: .. Protein binding microarray chips comprised of ≈20 000 individual human GST (glutathione S-transferase)- and His-tagged full-length proteins were obtained from the Johns Hopkins Medical Institutions Protein Microarray Core (CDI Laboratories, Inc). .. Microarray proteomic analyses were performed according to the procedure detailed below, and the data were processed24 by Wayen Biotechnologies Inc (Shanghai, China).

    Article Title: Deciphering the autoreactome: Massively parallelized methods for autoantibody detection in humans.
    Article Snippet: Autoantibodies have a substantial impact on human health ranging from autoimmune diseases to cancer diagnostics.. Knowledge of the antigens recognized can allow for more accurate diagnostics, a better understanding of pathogeneses and thus improved prevention, as well as laying the foundation for the development of new therapies.. A critical step to acquire this knowledge is to detect the exact self-antigens targeted by autoantibodies out of the pool of 20,000 human proteins against which reactivities could be observed.

    Article Title: The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-κB2 activation.
    Article Snippet: .. Protein array screening of US31-binding proteins The Johns Hopkins Medical Institutions Protein Microarray Core produced the protein binding microarray chips with 19394 individual human GST-and His6tagged full-length proteins. .. Recombinant US31 purified from BL21/pET21a-US31 (1.5 μg/μl) was labeled using an Alexa-Flour-647 microscale protein labeling kit (A30009, Molecular Probes/Invitrogen).

    Article Title: Targeting HINT1 to improve synaptic plasticity: toward loganin as a new antidepressant strategy.
    Article Snippet: Histidine triad nucleotide-binding protein 1 (HINT1) is related to depression.. However, the underlying mechanisms and whether HINT1 is a therapeutic target for depression remain unclear.. In this study, we report that loganin, an antidepressant candidate from our previous research, directly targets HINT1 to alleviate depressive-like behaviors.

    Protein Binding:

    Article Title: E17241 as a Novel ABCA1 (ATP-Binding Cassette Transporter A1) Upregulator Ameliorates Atherosclerosis in Mice
    Article Snippet: .. Protein binding microarray chips comprised of ≈20 000 individual human GST (glutathione S-transferase)- and His-tagged full-length proteins were obtained from the Johns Hopkins Medical Institutions Protein Microarray Core (CDI Laboratories, Inc). .. Microarray proteomic analyses were performed according to the procedure detailed below, and the data were processed24 by Wayen Biotechnologies Inc (Shanghai, China).

    Article Title: The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-κB2 activation.
    Article Snippet: .. Protein array screening of US31-binding proteins The Johns Hopkins Medical Institutions Protein Microarray Core produced the protein binding microarray chips with 19394 individual human GST-and His6tagged full-length proteins. .. Recombinant US31 purified from BL21/pET21a-US31 (1.5 μg/μl) was labeled using an Alexa-Flour-647 microscale protein labeling kit (A30009, Molecular Probes/Invitrogen).

    Diagnostic Assay:

    Article Title: Identification of Novel Biomarkers for Behcet Disease Diagnosis Using Human Proteome Microarray Approach
    Article Snippet: .. This study was approved by the Ethics Committee of Peking Union Medical College Hospital. table ft1 table-wrap mode="anchored" t5 caption a7 Diagnostic symptom Positive number/ total(positive rate) Other symptom Positive number/ total(positive rate) Oral ulcers 110/113(97.3%) Untreated new-onset BD 59/113(52.2%) Genital ulcers 81/113(71.7%) Arthritis 22/107(20.6%) Eye lesions 28/113(24.78%) Epididymitis 8/105(7.6%) Skin lesions 68/113(60.18%) Gastrointestinal lesions 23/109(21.1%) Positive pathergy test 31/73(42.47%) Central nervous symptoms 15/112(13.4%) Vascular lesions 21/105(20.0%) Cardiac lesions 7/101(6.9%) Respiratory lesions 2/104(1.9%) Open in a separate window caption a8 The Clinical symptoms information of the BD patients Construction and Quality Control Test of HuProt Arrays The HuProt arrays, comprised of∼20,000 unique full-length proteins were constructed in Dr. Zhu's laboratory at Johns Hopkins University School of Medicine. .. Briefly, human ORFs cloned into a yeast expression vector (pEGH-A) were induced to produce N-terminally tagged GST fusions in Saccharomyces cerevisiae under control of the galactose-inducible GAL1 promoter.

    Control:

    Article Title: Identification of Novel Biomarkers for Behcet Disease Diagnosis Using Human Proteome Microarray Approach
    Article Snippet: .. This study was approved by the Ethics Committee of Peking Union Medical College Hospital. table ft1 table-wrap mode="anchored" t5 caption a7 Diagnostic symptom Positive number/ total(positive rate) Other symptom Positive number/ total(positive rate) Oral ulcers 110/113(97.3%) Untreated new-onset BD 59/113(52.2%) Genital ulcers 81/113(71.7%) Arthritis 22/107(20.6%) Eye lesions 28/113(24.78%) Epididymitis 8/105(7.6%) Skin lesions 68/113(60.18%) Gastrointestinal lesions 23/109(21.1%) Positive pathergy test 31/73(42.47%) Central nervous symptoms 15/112(13.4%) Vascular lesions 21/105(20.0%) Cardiac lesions 7/101(6.9%) Respiratory lesions 2/104(1.9%) Open in a separate window caption a8 The Clinical symptoms information of the BD patients Construction and Quality Control Test of HuProt Arrays The HuProt arrays, comprised of∼20,000 unique full-length proteins were constructed in Dr. Zhu's laboratory at Johns Hopkins University School of Medicine. .. Briefly, human ORFs cloned into a yeast expression vector (pEGH-A) were induced to produce N-terminally tagged GST fusions in Saccharomyces cerevisiae under control of the galactose-inducible GAL1 promoter.

    Construct:

    Article Title: Identification of Novel Biomarkers for Behcet Disease Diagnosis Using Human Proteome Microarray Approach
    Article Snippet: .. This study was approved by the Ethics Committee of Peking Union Medical College Hospital. table ft1 table-wrap mode="anchored" t5 caption a7 Diagnostic symptom Positive number/ total(positive rate) Other symptom Positive number/ total(positive rate) Oral ulcers 110/113(97.3%) Untreated new-onset BD 59/113(52.2%) Genital ulcers 81/113(71.7%) Arthritis 22/107(20.6%) Eye lesions 28/113(24.78%) Epididymitis 8/105(7.6%) Skin lesions 68/113(60.18%) Gastrointestinal lesions 23/109(21.1%) Positive pathergy test 31/73(42.47%) Central nervous symptoms 15/112(13.4%) Vascular lesions 21/105(20.0%) Cardiac lesions 7/101(6.9%) Respiratory lesions 2/104(1.9%) Open in a separate window caption a8 The Clinical symptoms information of the BD patients Construction and Quality Control Test of HuProt Arrays The HuProt arrays, comprised of∼20,000 unique full-length proteins were constructed in Dr. Zhu's laboratory at Johns Hopkins University School of Medicine. .. Briefly, human ORFs cloned into a yeast expression vector (pEGH-A) were induced to produce N-terminally tagged GST fusions in Saccharomyces cerevisiae under control of the galactose-inducible GAL1 promoter.

    Protein Array:

    Article Title: The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-κB2 activation.
    Article Snippet: .. Protein array screening of US31-binding proteins The Johns Hopkins Medical Institutions Protein Microarray Core produced the protein binding microarray chips with 19394 individual human GST-and His6tagged full-length proteins. .. Recombinant US31 purified from BL21/pET21a-US31 (1.5 μg/μl) was labeled using an Alexa-Flour-647 microscale protein labeling kit (A30009, Molecular Probes/Invitrogen).

    Produced:

    Article Title: The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-κB2 activation.
    Article Snippet: .. Protein array screening of US31-binding proteins The Johns Hopkins Medical Institutions Protein Microarray Core produced the protein binding microarray chips with 19394 individual human GST-and His6tagged full-length proteins. .. Recombinant US31 purified from BL21/pET21a-US31 (1.5 μg/μl) was labeled using an Alexa-Flour-647 microscale protein labeling kit (A30009, Molecular Probes/Invitrogen).



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    Hierarchical clustering of DEGs in leaves and roots of transgenic cotton T-34 compared with wild-type Z35 in the <t>microarray</t> analysis. Each column represents a single biological replicate and each row represents a differentially expressed probe set. L1, L2, and L3 represent biological replicates from leaves and R1, R2, and R3 represent biological replicates from roots. S/S represented self-to-self of Z35. The signal ratios were shown in a red–green colour scale, where red indicated up-regulation and green indicated down-regulation.
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    Image Search Results


    Hierarchical clustering of DEGs in leaves and roots of transgenic cotton T-34 compared with wild-type Z35 in the microarray analysis. Each column represents a single biological replicate and each row represents a differentially expressed probe set. L1, L2, and L3 represent biological replicates from leaves and R1, R2, and R3 represent biological replicates from roots. S/S represented self-to-self of Z35. The signal ratios were shown in a red–green colour scale, where red indicated up-regulation and green indicated down-regulation.

    Journal: Journal of Experimental Botany

    Article Title: Transcriptome analysis of Hpa1 Xoo transformed cotton revealed constitutive expression of genes in multiple signalling pathways related to disease resistance

    doi: 10.1093/jxb/erq227

    Figure Lengend Snippet: Hierarchical clustering of DEGs in leaves and roots of transgenic cotton T-34 compared with wild-type Z35 in the microarray analysis. Each column represents a single biological replicate and each row represents a differentially expressed probe set. L1, L2, and L3 represent biological replicates from leaves and R1, R2, and R3 represent biological replicates from roots. S/S represented self-to-self of Z35. The signal ratios were shown in a red–green colour scale, where red indicated up-regulation and green indicated down-regulation.

    Article Snippet: The 12k cDNA microarray was conduced at CapitalBio Corp. (Beijing, China) using the method described by Shi et al. (2006) .

    Techniques: Transgenic Assay, Microarray

    Correlation coefficients of microarray hybridization with total RNA from leaves of transgenic cotton T-34 and wild-type Z35

    Journal: Journal of Experimental Botany

    Article Title: Transcriptome analysis of Hpa1 Xoo transformed cotton revealed constitutive expression of genes in multiple signalling pathways related to disease resistance

    doi: 10.1093/jxb/erq227

    Figure Lengend Snippet: Correlation coefficients of microarray hybridization with total RNA from leaves of transgenic cotton T-34 and wild-type Z35

    Article Snippet: The 12k cDNA microarray was conduced at CapitalBio Corp. (Beijing, China) using the method described by Shi et al. (2006) .

    Techniques: Microarray, Hybridization, Transgenic Assay

    The functional annotation of 530 DEGs in leaves of transgenic T-34 identified in the microarray analysis ( P <0.001).

    Journal: Journal of Experimental Botany

    Article Title: Transcriptome analysis of Hpa1 Xoo transformed cotton revealed constitutive expression of genes in multiple signalling pathways related to disease resistance

    doi: 10.1093/jxb/erq227

    Figure Lengend Snippet: The functional annotation of 530 DEGs in leaves of transgenic T-34 identified in the microarray analysis ( P <0.001).

    Article Snippet: The 12k cDNA microarray was conduced at CapitalBio Corp. (Beijing, China) using the method described by Shi et al. (2006) .

    Techniques: Functional Assay, Transgenic Assay, Microarray

    Hierarchical clustering of DEGs in leaves and roots of transgenic cotton T-34 compared with wild-type Z35 in the microarray analysis. Each column represents a single biological replicate and each row represents a differentially expressed probe set. L1, L2, and L3 represent biological replicates from leaves and R1, R2, and R3 represent biological replicates from roots. S/S represented self-to-self of Z35. The signal ratios were shown in a red–green colour scale, where red indicated up-regulation and green indicated down-regulation.

    Journal: Journal of Experimental Botany

    Article Title: Transcriptome analysis of Hpa1 Xoo transformed cotton revealed constitutive expression of genes in multiple signalling pathways related to disease resistance

    doi: 10.1093/jxb/erq227

    Figure Lengend Snippet: Hierarchical clustering of DEGs in leaves and roots of transgenic cotton T-34 compared with wild-type Z35 in the microarray analysis. Each column represents a single biological replicate and each row represents a differentially expressed probe set. L1, L2, and L3 represent biological replicates from leaves and R1, R2, and R3 represent biological replicates from roots. S/S represented self-to-self of Z35. The signal ratios were shown in a red–green colour scale, where red indicated up-regulation and green indicated down-regulation.

    Article Snippet: Among 11 236 unigene ESTs included in the 12k cDNA microarray, 4.7% and 0.57% of ESTs were differentially expressed in leaves and roots of transgenic T-34, respectively.

    Techniques: Transgenic Assay, Microarray

    Correlation coefficients of microarray hybridization with total RNA from leaves of transgenic cotton T-34 and wild-type Z35

    Journal: Journal of Experimental Botany

    Article Title: Transcriptome analysis of Hpa1 Xoo transformed cotton revealed constitutive expression of genes in multiple signalling pathways related to disease resistance

    doi: 10.1093/jxb/erq227

    Figure Lengend Snippet: Correlation coefficients of microarray hybridization with total RNA from leaves of transgenic cotton T-34 and wild-type Z35

    Article Snippet: Among 11 236 unigene ESTs included in the 12k cDNA microarray, 4.7% and 0.57% of ESTs were differentially expressed in leaves and roots of transgenic T-34, respectively.

    Techniques: Microarray, Hybridization, Transgenic Assay

    The functional annotation of 530 DEGs in leaves of transgenic T-34 identified in the microarray analysis ( P <0.001).

    Journal: Journal of Experimental Botany

    Article Title: Transcriptome analysis of Hpa1 Xoo transformed cotton revealed constitutive expression of genes in multiple signalling pathways related to disease resistance

    doi: 10.1093/jxb/erq227

    Figure Lengend Snippet: The functional annotation of 530 DEGs in leaves of transgenic T-34 identified in the microarray analysis ( P <0.001).

    Article Snippet: Among 11 236 unigene ESTs included in the 12k cDNA microarray, 4.7% and 0.57% of ESTs were differentially expressed in leaves and roots of transgenic T-34, respectively.

    Techniques: Functional Assay, Transgenic Assay, Microarray