Journal: Cell Chemical Biology

Article Title: (ADP-ribosyl)hydrolases: Structural Basis for Differential Substrate Recognition and Inhibition

doi: 10.1016/j.chembiol.2018.11.001

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Article Snippet: 32 P-NAD + , PerkinElmer , NEG023X500UC.

Techniques: Recombinant, Software, Crystallization Assay

Journal: Scientific reports

Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

doi: 10.1038/s41598-022-14365-w

Figure Lengend Snippet: Figure 1. Characteristics of changes in serum levels of Sirt3 in patients with ICU. (a) Serum Sirt3 levels were measured in sepsis group, septic shock group, and ICU control group. (b) Serum Sirt3 levels on day1, day3, day5, and day7 in patients diagnosed with sepsis on day 1. (c) Serum Sirt3 levels on day1, day3, day5, and day7 in patients diagnosed with septic shock on day 1. (d) Serum Sirt3 levels were measured in sepsis group and septic shock group after re-diagnosed on D3 and D5. P < 0.05 were considered statistically significant. *denotes P < 0.05, **denotes P < 0.01, ****denotes P < 0.0001, “ns” means no significance.

Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

Techniques: Control

Journal: Scientific reports

Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

doi: 10.1038/s41598-022-14365-w

Figure Lengend Snippet: Figure 2. Correlation between Sirt3 and clinical indicators in ICU patients. (a) Correlation between serum Sirt3 and SOFA score in sepsis group, septic shock group, and ICU control group. (b) Correlation between serum Sirt3 and lactate in sepsis group, septic shock group, and ICU control group. (c) Correlation between serum Sirt3 and ScvO2 in sepsis group and septic shock group. (d) Correlation between serum Sirt3 and PCT in sepsis group and septic shock group. P < 0.05 were considered statistically significant. SOFA Sequential Organ Failure Assessment Score, ScvO2 Central venous oxygen saturation, PCT procalcitonin.

Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

Techniques: Control

Journal: Scientific reports

Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

doi: 10.1038/s41598-022-14365-w

Figure Lengend Snippet: Figure 3. The ROC curves of indicators for the diagnosis of sepsis and the early detection of sepsis from septic shock. (a) The ROC curves of indicators for the diagnosis of sepsis (PCT, Sirt3, SOFA). (b) The ROC curves of indicators for the early detection of septic shock (lactate, Scvo2, Sirt3, lactate + Sirt3, Lactate + ScvO2, P(V-A)CO2). Calculate the predicted values for lactate + Sirt3 and lactate + ScvO2 by binary logistic regression and then calculate the ROC curve. SOFA Sequential Organ Failure Assessment Score, ScvO2 Central venous oxygen saturation, PCT procalcitonin, P(V-A)CO2 central venous-to-arterial blood carbon dioxide partial pressure.

Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

Techniques: Biomarker Discovery

Journal: Scientific reports

Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

doi: 10.1038/s41598-022-14365-w

Figure Lengend Snippet: Figure 4. The ROC curves of predicting for 28-day survival and Kaplan–Meier estimator analysis of Sirt3 for 28 day survival. (a) The ROC curves of Sirt3 for the predicting 28-day mortality in patients with sepsis. P < 0.05 were considered statistically significant (b) Kaplan–Meier estimator analysis of Sirt3 for 28-day survival. The cut off value of Sirt3 for the estimated 28-day mortality of patients with sepsis was 10.07 pg/ml.

Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

Techniques: