Journal: Biosensors

Article Title: Fabrication of Co-Doped Covalent Organic Framework Nanosheets with Mild Interlayer Stress for Quantitative Detection of Alzheimer’s Disease Biomarkers

doi: 10.3390/bios16050271

Figure Lengend Snippet: ( A ) The amino-modified SPE, ( B ) process scheme for preparing GDH/Co-TPCOF/SPE, ( C ) schematic representation of the catalysis of NADH and glutamate by Co-TPCOF and GDH/Co-TPCOF.

Article Snippet: NADH, NAD + , and GDH were supplied by Sangon Biotech Co., Ltd. (Shanghai, China).

Techniques: Modification

Journal: Biosensors

Article Title: Fabrication of Co-Doped Covalent Organic Framework Nanosheets with Mild Interlayer Stress for Quantitative Detection of Alzheimer’s Disease Biomarkers

doi: 10.3390/bios16050271

Figure Lengend Snippet: DPV ( a ) and CV ( b ) curves of SPE and Co-TPCOF/SPE modified electrodes in 10 mM PBS (pH 7.0, the concentration of NADH was 5 mM); ( c ) DPV curves of GDH/Co-TPCOF/SPE, Co-TPCOF/SPE and SPE modified electrodes in 50 mM PBS (pH 8.0, the concentration of glutamate was 3 mM); ( d ) effects of pH in buffer solution; ( e ) GDH concentration in the label preparation; ( f ) Co-TPCOF concentration in the label preparation; EIS ( g ) and CV ( h ) analysis of different modified electrodes: (1) bare SPE, (2) GDH@Co-TPCOF/SPE, (3) after immobilization of Nafion.

Article Snippet: NADH, NAD + , and GDH were supplied by Sangon Biotech Co., Ltd. (Shanghai, China).

Techniques: Modification, Concentration Assay

Journal: Biosensors

Article Title: Fabrication of Co-Doped Covalent Organic Framework Nanosheets with Mild Interlayer Stress for Quantitative Detection of Alzheimer’s Disease Biomarkers

doi: 10.3390/bios16050271

Figure Lengend Snippet: ( a ) Amperometric i-t responses of the GDH/Co-TPCOF/SPE upon successive additions of NADH to PBS (10 mM, pH 7.0). The inset profile shows the response time; ( b ) calibration curves between the current change and concentration of NADH; ( c ) amperometric i-t responses of the GDH/Co-TPCOF/SPE upon successive additions of glutamate to PBS (50 mM, pH 8.0); the inset profile shows the response time; ( d ) calibration curves between the current change and concentration of glutamate; ( e ) selectivity of the glutamate in PBS (50 mM, pH 8.0) containing 1 mM of various interference species (glutamate, 3 mM); ( f ) selectivity of the NADH in PBS (10 mM, pH 7.0) containing 1 mM of various interference species (NADH, 3 mM).

Article Snippet: NADH, NAD + , and GDH were supplied by Sangon Biotech Co., Ltd. (Shanghai, China).

Techniques: Concentration Assay