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Millipore cisplatin
EPB41L5 promotes cell invasiveness of a tongue SCC cell line. a Expression of EPB41L5 in the tongue SCC cell lines SCC-9 and SCC-25 was examined by western blotting. b The invasive ability of each tongue SCC cell line was assessed by the Matrigel invasion assay. After 12 h of incubation, invaded cells were fixed and stained with crystal violet. The numbers of cells in six distinct regions of a single chamber were counted. Data are shown as means ± SEM. *** P < 0.001. c SCC-9 cells were transfected with two different sequences of siRNAs against EPB41L5 , and then the Matrigel invasion assay was performed. Data are means ± SEM. * P < 0.05. d Cell viabilities of SCC-9 cells with or without the silencing of EPB41L5 was assessed following the exposure to <t>cisplatin</t> and/or radiation. Cisplatin concentrations and radiation doses are as indicated. Data are shown as means ± SEM. *** P < 0.001
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FUJIFILM n 2 hydroxyethylpiperazine n ethanesulfonic acid hepes
EPB41L5 promotes cell invasiveness of a tongue SCC cell line. a Expression of EPB41L5 in the tongue SCC cell lines SCC-9 and SCC-25 was examined by western blotting. b The invasive ability of each tongue SCC cell line was assessed by the Matrigel invasion assay. After 12 h of incubation, invaded cells were fixed and stained with crystal violet. The numbers of cells in six distinct regions of a single chamber were counted. Data are shown as means ± SEM. *** P < 0.001. c SCC-9 cells were transfected with two different sequences of siRNAs against EPB41L5 , and then the Matrigel invasion assay was performed. Data are means ± SEM. * P < 0.05. d Cell viabilities of SCC-9 cells with or without the silencing of EPB41L5 was assessed following the exposure to <t>cisplatin</t> and/or radiation. Cisplatin concentrations and radiation doses are as indicated. Data are shown as means ± SEM. *** P < 0.001
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EPB41L5 promotes cell invasiveness of a tongue SCC cell line. a Expression of EPB41L5 in the tongue SCC cell lines SCC-9 and SCC-25 was examined by western blotting. b The invasive ability of each tongue SCC cell line was assessed by the Matrigel invasion assay. After 12 h of incubation, invaded cells were fixed and stained with crystal violet. The numbers of cells in six distinct regions of a single chamber were counted. Data are shown as means ± SEM. *** P < 0.001. c SCC-9 cells were transfected with two different sequences of siRNAs against EPB41L5 , and then the Matrigel invasion assay was performed. Data are means ± SEM. * P < 0.05. d Cell viabilities of SCC-9 cells with or without the silencing of EPB41L5 was assessed following the exposure to cisplatin and/or radiation. Cisplatin concentrations and radiation doses are as indicated. Data are shown as means ± SEM. *** P < 0.001

Journal: Cell Communication and Signaling : CCS

Article Title: High expression of EPB41L5, an integral component of the Arf6-driven mesenchymal program, correlates with poor prognosis of squamous cell carcinoma of the tongue

doi: 10.1186/s12964-016-0151-0

Figure Lengend Snippet: EPB41L5 promotes cell invasiveness of a tongue SCC cell line. a Expression of EPB41L5 in the tongue SCC cell lines SCC-9 and SCC-25 was examined by western blotting. b The invasive ability of each tongue SCC cell line was assessed by the Matrigel invasion assay. After 12 h of incubation, invaded cells were fixed and stained with crystal violet. The numbers of cells in six distinct regions of a single chamber were counted. Data are shown as means ± SEM. *** P < 0.001. c SCC-9 cells were transfected with two different sequences of siRNAs against EPB41L5 , and then the Matrigel invasion assay was performed. Data are means ± SEM. * P < 0.05. d Cell viabilities of SCC-9 cells with or without the silencing of EPB41L5 was assessed following the exposure to cisplatin and/or radiation. Cisplatin concentrations and radiation doses are as indicated. Data are shown as means ± SEM. *** P < 0.001

Article Snippet: After a 24 h incubation, cells were treated with 50 nM of cisplatin (Sigma), followed by an X-ray irradiation (MBR-1520R-3 HITACHI, 150 kV with a 0.5 mm aluminum filter.

Techniques: Expressing, Western Blot, Invasion Assay, Incubation, Staining, Transfection