Journal: Journal of translational medicine

Article Title: Circadian rhythm disruption upregulating Per1 in mandibular condylar chondrocytes mediating temporomandibular joint osteoarthritis via GSK3β/β-CATENIN pathway.

doi: 10.1186/s12967-024-05475-2

Figure Lengend Snippet: Fig. 2 Chronic circadian rhythm disruption leads to increased expression of the clock gene Per1 and matrix degrading enzymes in the mandibular condylar cartilage. A Relative mRNA expression curves of core clock genes in the mandibular condylar cartilage over a 24-h period (ZT0, ZT4, ZT8, ZT12, ZT16, ZT20 and ZT24) of two groups. B Western blot images of PER1, MMP13, ADAMTS4, and ADAMTS5 in mandibular condylar cartilage tissues in two groups. C Quantitative analysis of Western blot results. D Representative slices of immunohistochemical staining in the two groups. E Quantitative analysis of AOD in immunohistochemical staining of the two groups. n = 5, *P < 0.05. **P < 0.01

Article Snippet: Antigen retrieval was performed by incubating the sections in 1 mM EDTA antigen retrieval solution (pH = 9) in a water bath at 95 °C for 45 min. After rinsing with PBS, the sections were blocked with 5% BSA at room temperature for 1 h. The sections were then incubated with primary antibodies MMP13 (1:600, ProteinTech, China), ADAMTS4 (1:100, Affinity, USA), ADAMTS5 (1:100, Affinity, USA), and β-CATENIN (1:1000, ProteinTech, China) at 4 °C overnight.

Techniques: Disruption, Expressing, Western Blot, Immunohistochemical staining, Staining

Journal: Journal of translational medicine

Article Title: Circadian rhythm disruption upregulating Per1 in mandibular condylar chondrocytes mediating temporomandibular joint osteoarthritis via GSK3β/β-CATENIN pathway.

doi: 10.1186/s12967-024-05475-2

Figure Lengend Snippet: Fig. 6 Downregulation of Per1 in rat mandibular condylar cartilage can inhibit the activation of the GSK3β/β-CATENIN pathway and the increase in matrix-degrading enzymes caused by chronic circadian rhythm disruption. A Representative sections of immunohistochemical staining of PER1, MMP13, ADAMTS4, ADAMTS5 and β-CATENIN in mandibular condylar cartilage. B Average optical density of protein in condylar cartilage tissue. C Western blot results of PER1, MMP13, ADAMTS4, ADAMTS5, GSK3β, p-GSK3β, and β-CATENIN in the mandibular condylar cartilage of rats in four groups. D Statistical analysis of Western blot results in the condylar cartilage tissue of rats in the four groups. E Statistical analysis of p-GSK3β/GSK3β. n = 5, *P < 0.05, **P < 0.01

Article Snippet: Antigen retrieval was performed by incubating the sections in 1 mM EDTA antigen retrieval solution (pH = 9) in a water bath at 95 °C for 45 min. After rinsing with PBS, the sections were blocked with 5% BSA at room temperature for 1 h. The sections were then incubated with primary antibodies MMP13 (1:600, ProteinTech, China), ADAMTS4 (1:100, Affinity, USA), ADAMTS5 (1:100, Affinity, USA), and β-CATENIN (1:1000, ProteinTech, China) at 4 °C overnight.

Techniques: Activation Assay, Disruption, Immunohistochemical staining, Staining, Western Blot

Journal: Molecular medicine reports

Article Title: MEK inhibitor enhanced the antitumor effect of oxaliplatin and 5‑fluorouracil in MEK1 Q56P‑mutant colorectal cancer cells.

doi: 10.3892/mmr.2018.9730

Figure Lengend Snippet: Figure 4. Gene expression induced by U0126 combined with or without oxaliplatin/5‑FU. (A) ERCC1 mRNA levels in SW48 cells were examined by RT‑qPCR following treatment with U0126 and/or oxaliplatin. (B) TYMS mRNA levels in SW48 cells were examined by RT‑qPCR following treatment with U0126 and/or 5‑FU. The graph depicts the fold change in ERCC1/TYMS levels normalized to β‑actin levels. Data are presented as means ± standard deviation. Western blot analysis results of (C) ERCC1 and (D) TYMS protein expression levels in cells treated with U0126 in combination with oxaliplatin or 5‑FU, respectively. **P<0.01 vs. control group; ##P<0.01 vs. oxaliplatin/5‑FU‑treated group. 5‑FU, 5‑fluorouracil; ERCC1, excision repair cross‑complementation group 1; TYMS, thymidylate synthase; RT‑qPCR, reverse transcription‑quantitative polymerase chain reaction.

Article Snippet: Excision repair cross-complementation group 1 (ERCC1) rabbit polyclonal antibody (cat. no. 14586‐1‐AP) and TYMS rabbit polyclonal antibody (cat. no. 15047‐1‐AP) were purchased from ProteinTech Group, Inc. (Wuhan, China).

Techniques: Gene Expression, Standard Deviation, Western Blot, Expressing, Control, Polymerase Chain Reaction

Journal: Cell metabolism

Article Title: Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue

doi: 10.1016/j.cmet.2022.02.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CD86 Antibody, anti-mouse, FITC, Miltenyi Biotec , Miltenyi Biotec , Cat# 130–123-672; RRID:AB_2889633.

Techniques: Immunofluorescence, Recombinant, Cytometry, Purification, shRNA, Cell Culture, Lysis, XF Assay, Staining, Isolation, Transfection, Live Cell Imaging, Mouse Assay, Software, Expressing

Journal: Journal of translational medicine

Article Title: Harm of circadian misalignment to the hearts of the adolescent wistar rats.

doi: 10.1186/s12967-022-03546-w

Figure Lengend Snippet: Fig. 5 A The Flameng score of myocardial mitochondria measured using a fluoroscopy electron fiberscope. Expression of CD34 (B), HSP60 (C), CACNA1C (D) and BECLIN-1 (E) in the two groups of rats measured by immunofluorescence staining. *p < 0.05, **p < 0.01, ***p < 0.001 (two-sample t-test). (F) Transmission electron microscope scanning images of the myocardial mitochondria from the two groups of rats. A large number of myocardial mitochondria in the CM group showed vacuolar changes and cristae fractures, indicating a significant increase in ruptured mitochondria. Immunofluorescence staining for CD34 (G), HSP60 (H), CACNA1C (I) and BECLIN-1 (J). Blue represents the nucleus stained with DAPI, and red represents positive expression. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; HSP60, heat shock protein 60; CACNA1C, Calcium Voltage-Gated Channel Subunit Alpha1 C; BECLIN-1, Coiled-Coil, Moesin-Like BCL2-Interacting Protein

Article Snippet: This experiment was performed to observe the relative expression of the following proteins in myocardial tissue: TNF-α (bsm-33207 m; Bioss), sprouty RTK signaling antagonist 2 (SPRY2; GB11860; Servicebio), receptor for advanced glycosylation end-product specific (RAGE; 16,346–1-AP; Proteintech), lamin B1 (GB1111802; Servicebio), lamin A/C (GB11407, Servicebio), IL-6 (GB11117, Servicebio) and CACNA1C (21774-1-ap; proteintech).

Techniques: Expressing, Immunofluorescence, Staining, Transmission Assay, Microscopy

Journal: Journal of translational medicine

Article Title: Harm of circadian misalignment to the hearts of the adolescent wistar rats.

doi: 10.1186/s12967-022-03546-w

Figure Lengend Snippet: Fig. 6 Relative expression levels of TNF-a (A), RAGE (B), SPRY2 (C), CACNA1C (D), IL-6 (E), lamin A/C (F), and lamin B1 (G) in the apical tissues of rats from the two groups, measured by western blotting. *p < 0.05, **p < 0.01, ***p < 0.001 (two-sample t-test). H Bands of the above proteins. Abbreviations: IL-6, interleukin-6; RAGE, receptor for advanced glycosylation end-product specific; SPRY2, sprouty RTK signaling antagonist 2; TNF-a, tumor necrosis factor-alpha; CACNA1C, Calcium Voltage-Gated Channel Subunit Alpha1 C

Article Snippet: This experiment was performed to observe the relative expression of the following proteins in myocardial tissue: TNF-α (bsm-33207 m; Bioss), sprouty RTK signaling antagonist 2 (SPRY2; GB11860; Servicebio), receptor for advanced glycosylation end-product specific (RAGE; 16,346–1-AP; Proteintech), lamin B1 (GB1111802; Servicebio), lamin A/C (GB11407, Servicebio), IL-6 (GB11117, Servicebio) and CACNA1C (21774-1-ap; proteintech).

Techniques: Expressing, Western Blot, Glycoproteomics