Journal: Molecular pharmaceutics

Article Title: Tocopherol Emulsions as Functional Autoantigen Delivery Vehicles Evoke Therapeutic Efficacy in Experimental Autoimmune Encephalomyelitis

doi: 10.1021/acs.molpharmaceut.8b00887

Figure Lengend Snippet: Formulation of a tocopherol-based vehicle for antigen delivery. A.) Emulsion fabrication using varied proportions of Vitamin E, TPGS and PBS enabled the construction of a ternary phase diagram, where scale denotes percent composition of each component. B.) Favorable component proportions were used to fabricate emulsions both with (ETPGS+PLP) and without (ETPGS) PLP for sizing via dynamic light scattering (n = 3/group) DLS sizes and half-widths are presented in terms of average size ± standard deviation. C.) formulations of ETPGS alone (left) and ETPGS+PLP (right) were also imaged with TEM to verify antigen incorporation into the emulsion. The lower right scale bar represents 500 nm for both formulations.

Article Snippet: Next, the tube containing α-tocopherol, TPGS and PBS (with or without PLP) was sonicated using a Fisher Scientific™ Sonic Dismembrator Model 500 with a 10 second on , 2 second off cadence (30% intensity) for 2 minutes, dipping the emulsion mixture in an ice bath during off periods to maintain temperature.

Techniques: Standard Deviation

Journal: Pharmaceutics

Article Title: Spanlastics as a Potential Platform for Enhancing the Brain Delivery of Flibanserin: In Vitro Response-Surface Optimization and In Vivo Pharmacokinetics Assessment

doi: 10.3390/pharmaceutics14122627

Figure Lengend Snippet: Levels of independent variables and desirable constraints of the responses for the response-surface randomized D-optimal design employed for FLB-SPLs’ formulations.

Article Snippet: The other materials—Span ® 60, Tween ® 80, sodium deoxycolate (SDC), polyvinyl alcohol (PVA), D-α-tocopheryl polyethylene glycol succinate (TPGS), and solvents—were procured from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany).

Techniques: Sonication, Zeta Potential Analyzer

Journal: Pharmaceutics

Article Title: Spanlastics as a Potential Platform for Enhancing the Brain Delivery of Flibanserin: In Vitro Response-Surface Optimization and In Vivo Pharmacokinetics Assessment

doi: 10.3390/pharmaceutics14122627

Figure Lengend Snippet: Composition of FLB-SPLs’ experimental runs, prepared according to the response-surface randomized D-optimal design, and their relative responses.

Article Snippet: The other materials—Span ® 60, Tween ® 80, sodium deoxycolate (SDC), polyvinyl alcohol (PVA), D-α-tocopheryl polyethylene glycol succinate (TPGS), and solvents—were procured from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany).

Techniques:

Journal: Pharmaceutics

Article Title: Spanlastics as a Potential Platform for Enhancing the Brain Delivery of Flibanserin: In Vitro Response-Surface Optimization and In Vivo Pharmacokinetics Assessment

doi: 10.3390/pharmaceutics14122627

Figure Lengend Snippet: Linear plots showing individual effects on FLB-SPLs’ size. ( A ) Span: EA ratio; ( B ) ST; ( C ) EA type. Abbreviations: FLB, flibanserin; SPL, spanlastics; EA, edge activator; ST, sonication time; PVA, polyvinyl alcohol; SDC, sodium deoxycholate; TPGS, D-α-tocopheryl polyethylene glycol succinate.

Article Snippet: The other materials—Span ® 60, Tween ® 80, sodium deoxycolate (SDC), polyvinyl alcohol (PVA), D-α-tocopheryl polyethylene glycol succinate (TPGS), and solvents—were procured from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany).

Techniques: Sonication

Journal: Pharmaceutics

Article Title: Spanlastics as a Potential Platform for Enhancing the Brain Delivery of Flibanserin: In Vitro Response-Surface Optimization and In Vivo Pharmacokinetics Assessment

doi: 10.3390/pharmaceutics14122627

Figure Lengend Snippet: Linear plots showing individual effects on FLB-SPLs’ zeta potential. ( A ) Span: EA ratio, ( B ) ST; ( C ) EA type. Abbreviations: FLB, flibanserin; SPL, spanlastics; EA, edge activator; ST, sonication time; PVA, polyvinyl alcohol; SDC, sodium deoxycholate; TPGS, D-α-tocopheryl polyethylene glycol succinate.

Article Snippet: The other materials—Span ® 60, Tween ® 80, sodium deoxycolate (SDC), polyvinyl alcohol (PVA), D-α-tocopheryl polyethylene glycol succinate (TPGS), and solvents—were procured from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany).

Techniques: Zeta Potential Analyzer, Sonication

Journal: Pharmaceutics

Article Title: Spanlastics as a Potential Platform for Enhancing the Brain Delivery of Flibanserin: In Vitro Response-Surface Optimization and In Vivo Pharmacokinetics Assessment

doi: 10.3390/pharmaceutics14122627

Figure Lengend Snippet: ( A ) Ramp graphs for the optimized variable levels and predicted responses of the optimized FLB-SPLs; ( B ) bar graph for the desirability of the optimization process. Abbreviations: FLB, flibanserin; SPL, spanlastics; EA, edge activator; ST, sonication time; PVA, polyvinyl alcohol; SDC, sodium deoxycholate; TPGS, D-α-tocopheryl polyethylene glycol succinate; PS, particle size; ZP, zeta potential.

Article Snippet: The other materials—Span ® 60, Tween ® 80, sodium deoxycolate (SDC), polyvinyl alcohol (PVA), D-α-tocopheryl polyethylene glycol succinate (TPGS), and solvents—were procured from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany).

Techniques: Sonication, Zeta Potential Analyzer