Journal: PLoS ONE

Article Title: All-Trans Retinoic Acid in Combination with Primaquine Clears Pneumocystis Infection

doi: 10.1371/journal.pone.0053479

Figure Lengend Snippet: Immunosuppressed, Pc-infected mice were treated with vehicle control 8% DMSO, ATRA, or TMP-SMX for 3, 4, and 5 weeks as indicated in the figure. Treatment was initiated 3 weeks after Pc inoculation. ( A ) Giemsa-stained BAL cells cytospun on slides. Red arrows indicate MDSC-like cells, and blue arrows denote alveolar macrophages. ( B ) Representative flow cytograms of BAL cells from mice in each group at each time point. The percentage of Gr-1 + /CD11b + cells in each sample is shown in the right upper quadrant of each flow cytogram. ( C ) Percentage of Gr-1 + /CD11b + cells in BALF, as determined by flow cytometry. Data are means ± S. D. of 3–6 mice in each group at each time point. (D) Histologic sections of the lungs stained with GMS (left) and H&E (right). Red arrows indicate GMS-stained Pc organisms. Images are representative of 3–6 mice in each group at each time point. Microscope magnification: 40X.

Article Snippet: TMP-SMX (Septra) was purchased from Hi-Tech Pharmacal (Amityville).

Techniques: Infection, Staining, Flow Cytometry, Microscopy

Journal: PLoS ONE

Article Title: All-Trans Retinoic Acid in Combination with Primaquine Clears Pneumocystis Infection

doi: 10.1371/journal.pone.0053479

Figure Lengend Snippet: Immunosuppressed, Pc-infected mice were given vehicle control 8% DMSO, ATRA-PMQ, or TMP-SMX for 2 weeks. Treatment was initiated 3 weeks after Pc inoculation. (A) Lung impression smear stained with Giemsa. Red arrows indicate nuclei of Pc organisms. Magnification: 100X. (B) Giemsa-stained BAL cells cytospun on slides. Black arrows indicate MDSC-like cells. Microscope magnification: 40X. Images are representative of 3–6 mice of each group.

Article Snippet: TMP-SMX (Septra) was purchased from Hi-Tech Pharmacal (Amityville).

Techniques: Infection, Staining, Microscopy

Journal: PLoS ONE

Article Title: All-Trans Retinoic Acid in Combination with Primaquine Clears Pneumocystis Infection

doi: 10.1371/journal.pone.0053479

Figure Lengend Snippet: Immunosuppressed, Pc-infected rats were given vehicle control 8% DMSO, ATRA, ATRA-PMQ, or TMP-SMX for 4 weeks. Treatment was initiated 2 weeks after Pc inoculation. BAL cells were examined by flow cytometry. The percentage of His48 + /CD11bc + MDSCs in each sample is shown in the right upper quadrant of each flow cytogram.

Article Snippet: TMP-SMX (Septra) was purchased from Hi-Tech Pharmacal (Amityville).

Techniques: Infection, Flow Cytometry

Journal: PLoS ONE

Article Title: All-Trans Retinoic Acid in Combination with Primaquine Clears Pneumocystis Infection

doi: 10.1371/journal.pone.0053479

Figure Lengend Snippet: Immunosuppressed, Pc-infected mice were given vehicle control 8% DMSO, ATRA, ATRA-PMQ, or TMP-SMX for 5 weeks. Treatment was initiated 3 weeks after Pc inoculation. BAL cells were collected and analyzed by flow cytometry with fluorescence labeled anti-mouse CD11c antibody. ( A ) Representative flow cytograms of BAL cells from animals of each group. ( B ) Percentage of CD11c + BAL cells in BALF, as determined by flow cytometry. Data are means ± S. D. of 3–6 mice in each group.

Article Snippet: TMP-SMX (Septra) was purchased from Hi-Tech Pharmacal (Amityville).

Techniques: Infection, Flow Cytometry, Fluorescence, Labeling

Journal: PLoS ONE

Article Title: All-Trans Retinoic Acid in Combination with Primaquine Clears Pneumocystis Infection

doi: 10.1371/journal.pone.0053479

Figure Lengend Snippet: Immunosuppressed, Pc-infected mice were treated with ATRA-PMQ or TMP-SMX for 2 weeks and then examined for the presence of Pc at 3 weeks after initiation of treatment and at 3 weeks after cessation of the three-week treatment. Lung tissues were subjected to Pc mitochondrial rRNA PCR, and the PCR products were electrophoresed on a 1% agarose gel.

Article Snippet: TMP-SMX (Septra) was purchased from Hi-Tech Pharmacal (Amityville).

Techniques: Infection, Agarose Gel Electrophoresis

Journal: The Cochrane Database of Systematic Reviews

Article Title: Antibiotic prophylaxis for preventing burn wound infection

doi: 10.1002/14651858.CD008738.pub2

Figure Lengend Snippet: Kimura 1998

Article Snippet: Notes , Sources of support: Quote: "We are indebted to MI. Yasuji Aoto, laboratory technician at Nippon Medieal Sehool Hospital, for collecting bacterial strains, and Shionogi Pharmaceutical Company for technical assistance in measuring TMP‐SMX concentrations" (Page 386). , .

Techniques: Control, Serial Time-encoded Amplified Microscopy, Infection, Cell Counting, Bacteria, Sequencing, Selection