Journal: International Journal of Nanomedicine

Article Title: Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity

doi: 10.2147/IJN.S70919

Figure Lengend Snippet: Genes regulated by Taxotere ® and docetaxel-loaded solid lipid nanoparticles were confirmed by quantitative polymerase chain reaction and immunoblotting. Notes: Cell cycle-related genes of E2F8 ( A ) and OIP5 ( B ), proliferation-related genes of NASP ( C ) and SOD2 ( D ), and apoptosis-related genes of PDCD4 ( E ) and PIK3R2 ( F ) were chosen for detection by quantitative polymerase chain reaction and immunoblotting. In quantitative polymerase chain reaction detection, mock-treated cells were set as the control and samples were normalized with the control. In immunoblotting detection, β-actin was used as the loading control. Abbreviations: BSN, blank solid lipid nanoparticle; DSN, docetaxel-loaded solid lipid nanoparticle; GLU, glucose; qPCR, quantitative polymerase chain reaction; TAX, Taxotere.

Article Snippet: Primary antibodies of rabbit anti-β-actin, E2f8, MRE11A, ERBB3, IGFBP6, ATF3, CCNG2, SOD2, IGFBP3, CADM1, PDCD4, GADD45A, and MKI67 were purchased from Beijing Biosynthesis Technology Co., Ltd., (Beijing, People’s Republic of China), rabbit anti-MCM6, OIP5, and NASP were purchased from Proteintech Group, Inc., (Chicago, IL, USA), rabbit anti-FAM172A and MYB were purchased from Abgent, Inc. (San Diego, CA, USA) and rabbit anti-ATRX was purchased from GeneTex, Inc. (Irvine, CA, USA).

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Control

Journal: International Journal of Nanomedicine

Article Title: Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity

doi: 10.2147/IJN.S70919

Figure Lengend Snippet: Primers used for quantitative polymerase chain reaction

Article Snippet: Primary antibodies of rabbit anti-β-actin, E2f8, MRE11A, ERBB3, IGFBP6, ATF3, CCNG2, SOD2, IGFBP3, CADM1, PDCD4, GADD45A, and MKI67 were purchased from Beijing Biosynthesis Technology Co., Ltd., (Beijing, People’s Republic of China), rabbit anti-MCM6, OIP5, and NASP were purchased from Proteintech Group, Inc., (Chicago, IL, USA), rabbit anti-FAM172A and MYB were purchased from Abgent, Inc. (San Diego, CA, USA) and rabbit anti-ATRX was purchased from GeneTex, Inc. (Irvine, CA, USA).

Techniques: Sequencing

Journal: Experimental and Therapeutic Medicine

Article Title: LSKL peptide alleviates subarachnoid fibrosis and hydrocephalus by inhibiting TSP1-mediated TGF-β1 signaling activity following subarachnoid hemorrhage in rats

doi: 10.3892/etm.2016.3640

Figure Lengend Snippet: LSKL peptide inhibited TSP1-mediated TGF-β1 signaling activity following SAH. Quantitative analyses of (A) TSP1 and (B) total TGF-β1 and active TGF-β1 in the CSF on days 3–5 after SAH. (C) Ratio of active TGF-β1 to total TGF-β1 in the CSF on days 3–5 after SAH. (D) Representative western blot bands of p-Smad2/3 and (E) quantitative analyses of p-Smad2/3 expression on day 5 after SAH. Relative densities of each protein have been normalized against the sham group. Data are expressed as the mean ± standard error of the mean (n=10). *P<0.05 vs. the sham group; # P<0.05 vs. the SAH+PBS group. SAH, subarachnoid hemorrhage; CSF, cerebrospinal fluid; TSP1, thrombospondin-1; LSKL, leucine-serine-lysine-leucine; PBS, phosphate buffer solution.

Article Snippet: These CSF samples were mixed and divided into three equal shares for the detection of TSP1, activated TGF-β1 and total TGF-β1 using the respective ELISA kit (Wuhan Boster Biological Technology, Ltd., Wuhan, China).

Techniques: Activity Assay, Western Blot, Expressing