scanners Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Siemens Healthineers pet ct scanner
    The original lesions in right adrenal gland and false-positive images in various bone segments of 2 years and 9 months old girl (patient 1). a DWIBS (anterior). b DWIBS (lateral). c 123 I-MIBG scintigraphy/SPECT (anterior). d 123 I-MIBG scintigraphy/SPECT (lateral). The MIP of 18 <t>F-FDG</t> <t>PET</t> ( e ) and DWIBS ( f ). Long arrows show the original lesion ( a – f ). Arrowheads show false-positive images ( a, b, e, f )
    Pet Ct Scanner, supplied by Siemens Healthineers, used in various techniques. Bioz Stars score: 99/100, based on 308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet ct scanner/product/Siemens Healthineers
    Average 99 stars, based on 308 article reviews
    Price from $9.99 to $1999.99
    pet ct scanner - by Bioz Stars, 2019-05
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies microarray scanner
    Correlation of <t>microarray</t> and qPCR data from fresh vs. frozen tissue. Correlation of microarray and qPCR results based on data from RNA extracted from fresh mouse brains in the DA TC and DR versus the use of RNA extracted from flash frozen brains in the PbTx TC. While minor differences were observed depending on the data set used, one data set did not consistently yield higher correlations. It does not appear that the use of frozen tissue, rather than fresh, appreciably influences the observed correlations with qPCR data. Asterisks indicate a statistically significant correlation (p
    Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 7037 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray scanner/product/Agilent technologies
    Average 99 stars, based on 7037 article reviews
    Price from $9.99 to $1999.99
    microarray scanner - by Bioz Stars, 2019-05
    99/100 stars
      Buy from Supplier

    94
    InDevR microarray scanner
    Reactivity of sera from experimentally infected mallards against recombinant HA in ELISA and IVPM. Infection and sample collection scheme for experimentally inoculated mallards ( A ). Absolute AUC values ( B ) and fold induction ( C ) over the AUC of naive sera against recombinant HA in ELISA are shown, calculated for each HA. ( D ) AUC and ( E ) fold induction data collected via the IVPM. ( F ) Reactivity of sera to chimeric H5/3 (cH5/3), H3 and H5 in ELISA. area under the curve, AUC; enzyme-linked immunosorbent assay, ELISA; hemagglutinin, HA; influenza virus protein <t>microarray,</t> IVPM.
    Microarray Scanner, supplied by InDevR, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray scanner/product/InDevR
    Average 94 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    microarray scanner - by Bioz Stars, 2019-05
    94/100 stars
      Buy from Supplier

    92
    Hamamatsu nanozoomer 2 0 ht digital slide scanner
    Characterization of Hb following preterm rabbit pup IVH . The presence of Hb following IVH was characterized within the brain utilizing the inherent peroxidase activity of Hb. Rabbit pups with IVH or sham controls were euthanized at 72 h of age followed by saline and freshly prepared 4% PFA perfusion. Afterwards the brains were dissected out from the skulls and immersed in 4% PFA. Brains were prepared and sections and a number of subventricular and periventricular anatomically comparable regions of interests (ROI) were chosen. Neuroanatomically defined ROI's located in the rostral forebrain at the levels of rostral forebrain (Level 1), caudal forebrain (Level 2), rostral midbrain (Level 3) and caudal midbrain (Level 4), were stained for peroxidase activity of Hb as described in the Materials and Methods Section. Microscope analyses were performed on a wide-field Olympus microscope (IX73) and slide scanning were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730. Scanning was performed with a 40 × magnification lens. Images used for illustrations, from regions of interest (ROI), were grabbed with the viewer software NDP.view2 Viewing software. Scale bar of slide scan image indicate 2.5 mm and of ROI images indicate 500 μm.
    Nanozoomer 2 0 Ht Digital Slide Scanner, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 92/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanozoomer 2 0 ht digital slide scanner/product/Hamamatsu
    Average 92 stars, based on 106 article reviews
    Price from $9.99 to $1999.99
    nanozoomer 2 0 ht digital slide scanner - by Bioz Stars, 2019-05
    92/100 stars
      Buy from Supplier

    88
    GE Healthcare phosphorimager scanner
    Compatible pollen triggers a decrease in S-RNase transcript levels by a post-transcriptional mechanism. (A) An S 11 -RNAse transgene containing the entire S11-RNase coding sequence and 3'UTR (white) under control of the style-specific chitinase gene promoter and 5'UTR (gray). (B) Northern blot analysis of S 12 S 14 plants expressing the transgenic S 11 -RNase, either without pollination, after a fully incompatible cross (× S 12 S 12 ) or after a fully compatible cross (× S 15 S 16 ) using gene probes against either the S 11 - or the S 12 -RNase transcript. (C) Levels of both S 11 - and S 12 -RNase transcripts (white and black bars, respectively) were quantified by <t>PhosphorImager</t> and reported relative to unpollinated styles. Values are means±s.d. of S-RNase transcript mRNA levels. Asterisks are significantly different (p
    Phosphorimager Scanner, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorimager scanner/product/GE Healthcare
    Average 88 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    phosphorimager scanner - by Bioz Stars, 2019-05
    88/100 stars
      Buy from Supplier

    87
    PerkinElmer confocal microarray scanner
    Expression profiling of subsets of SLE reflecting disease activity, using a 135 recombinant antibody <t>microarray.</t> A , Classification of SLE, grouped based solely on disease activity (SLEDAI values); low (3 to 6), mid (9 to 19) and high (22 to 34), based on all 135 antibodies, using a SVM-based leave-one-out cross validation test, expressed in terms of AUC values. AUC values obtained when using only significantly differentially expressed analytes are given within brackets. B , Significantly differentially expressed analytes are shown in a heat map. Twelve differentially expressed analytes, recognized by 14 antibodies, were identified for low versus mid; eight analytes, recognized by 12 antibodies for mid versus high; and 10 analytes recognized by 16 antibodies for low versus high. Green, down-regulated; red, up-regulated; and black, equal levels. The color represent the fold change of a particular marker across all samples within each sample cohort, calculated using the average signal intensities. C , Microarray signal intensities observed for complement proteins, including C1q, C3, C4, and C5, shown as boxplots. The median values are indicated (thick line) and the hinges represent the 25th percentile and the 75th percentile, respectively. D , The correlation between array signal intensities for C1q and SLE disease activity, expressed as SLEDAI-2K.
    Confocal Microarray Scanner, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 87/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/confocal microarray scanner/product/PerkinElmer
    Average 87 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    confocal microarray scanner - by Bioz Stars, 2019-05
    87/100 stars
      Buy from Supplier

    84
    Siemens AG hybrid pet mri scanner
    Case #4. Baseline magnetic resonance imaging <t>(MRI)</t> (A) , fluorothymidine (FLT)–positron-emission tomography <t>(PET)</t> (B) , and fused PET/MRI (C) . The 1.6-cm cerebellar lesion (arrow) with FLT avidity showed SUV ratio of 18.2.
    Hybrid Pet Mri Scanner, supplied by Siemens AG, used in various techniques. Bioz Stars score: 84/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid pet mri scanner/product/Siemens AG
    Average 84 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    hybrid pet mri scanner - by Bioz Stars, 2019-05
    84/100 stars
      Buy from Supplier

    83
    GE Healthcare new generation pet ct scanner
    D600 <t>PET/CT</t> vs <t>DMI</t> PET/CT: no additional bone lesions were detected by DMI PET/CT compared to standard PET/CT. 61 years-old man with tonsillar cancer who underwent PET/CT for evaluation of response to therapy. DMI images were acquired 47.4 minutes after standard acquisition and 126 minutes after 18 F-FDG injection. A) D600 MIP; B) DMI MIP; C) D600 sagittal fused image; D) DMI sagittal fused image.
    New Generation Pet Ct Scanner, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 83/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/new generation pet ct scanner/product/GE Healthcare
    Average 83 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    new generation pet ct scanner - by Bioz Stars, 2019-05
    83/100 stars
      Buy from Supplier

    82
    Philips Healthcare ingenuity tf pet mri scanner
    Comparison of lymph node staging (N-stage) on pre-transurethral resection ( a , b , c ) and post-chemotherapy 11 C-acetate <t>PET/MRI</t> ( d , e , f ) in patient number 12, the same patient as in Fig. 4 . 17 mm right obturator lymph node ( a - white arrow on T2-weighted image) demonstrated increased diffusion signal restriction ( b - b value 800 s/mm 2 trace diffusion weighted image) and increased 11 C-acetate uptake ( c - PET fused with T2-weighted image, SUV is scaled from 0.0 to 3.5) suggestive of lymph node metastasis. On post-chemotherapy 11 C-acetate PET/MRI, lymph node decreased in size and measured 4 mm ( d - green arrow on T2-weighted image) with increased diffusion signal ( e - b value 800 s/mm 2 trace diffusion weighted image) and increased 11 C-acetate uptake ( f - PET fused with T2-weighted image, SUV is scaled from 0.0 to 3.0). SUVmax values of the lymph node on the pre-transurethral resection ( c ) and post-chemotherapy 11 C-acetate PET/MRI ( f ) were 3.4 and 2.8, respectively. The lymph node was confirmed to be lymph node metastasis measuring 3 mm on extended pelvic lymph node dissection, thus the findings of 11 C-acetate PET/MRI were considered as true positive
    Ingenuity Tf Pet Mri Scanner, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ingenuity tf pet mri scanner/product/Philips Healthcare
    Average 82 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    ingenuity tf pet mri scanner - by Bioz Stars, 2019-05
    82/100 stars
      Buy from Supplier

    80
    Philips Healthcare whole body fdg pet mr scanner
    A 46-year-old woman with breast cancer. A μ-map of <t>FDG</t> <t>PET/MR</t> mammography ( a ) show body contour artifact with missing dorsal body contour including both lungs causing failure of attenuation correction on an axial PET image ( b )
    Whole Body Fdg Pet Mr Scanner, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/whole body fdg pet mr scanner/product/Philips Healthcare
    Average 80 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    whole body fdg pet mr scanner - by Bioz Stars, 2019-05
    80/100 stars
      Buy from Supplier

    79
    Hitachi Ltd confocal laser based fluorescence microarray scanner
    Schematic process for detection of CTCs on a cell <t>microarray</t> chip. (A) Human leukocytes/carcinoma cells are dispersed on a cell microarray chip, followed by 15 minutes' standing to allow the cells to settle down into the microchambers, and are then stained with fluorescence-labeled antibodies against carcinoma cells. (B) Fluorescence-positive CTCs are detected by using a microarray scanner with a confocal fluorescence laser. (C) The target CTCs are analyzed quantitatively at the single-cell level.
    Confocal Laser Based Fluorescence Microarray Scanner, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 79/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/confocal laser based fluorescence microarray scanner/product/Hitachi Ltd
    Average 79 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    confocal laser based fluorescence microarray scanner - by Bioz Stars, 2019-05
    79/100 stars
      Buy from Supplier

    78
    Mivenion nir scanner
    Monitoring of antithrombotic therapy in mice using FLECT/CT. Thrombus was induced in the left carotid artery of mice by ferric chloride before the i.v. injection of activated-platelet targeting <t>fluoroprobe,</t> Targ-Cy7. A) Representative comparison of maximum-intensity projection FLECT/CT images of mice before and after treatment with either PBS or combined thrombolytic/anti-platelet drugs, urokinase/integrilin (n=3). The colour scale for each FLECT/CT image shows levels of detected <t>NIR</t> fluorescence, with white corresponding to the highest intensity and blue the lowest. B) The injured carotid artery was collected from each treated animal and scanned on the IVIS ® Lumina imager to confirm the detected FLECT NIR signal. A representative image of both control (PBS-treated) and urokinase/integrilin-treated left carotid vessels is shown. Detected Lumina fluorescence signal is depicted accordingly.
    Nir Scanner, supplied by Mivenion, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nir scanner/product/Mivenion
    Average 78 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nir scanner - by Bioz Stars, 2019-05
    78/100 stars
      Buy from Supplier

    78
    Naviscan Inc pem scanner
    Phantom image from the <t>PEM/PET</t> system. (a) Picture of the mini-Derenzo phantom (the numbers shown on the picture are the diameters of the rods in millimetres) (b) PEM/PET image of the phantom.
    Pem Scanner, supplied by Naviscan Inc, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pem scanner/product/Naviscan Inc
    Average 78 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    pem scanner - by Bioz Stars, 2019-05
    78/100 stars
      Buy from Supplier

    77
    Siemens Healthineers somatom definition as 128 slice ct scanner
    13-year-old male with comminuted right orbital roof “blow-in” fracture. Unenhanced CT orbits (obtained with a Siemens <t>SOMATOM®</t> Definition AS 128-slice CT scanner, Siemens Healthcare, using bone algorithm; axial acquisition of 0.6
    Somatom Definition As 128 Slice Ct Scanner, supplied by Siemens Healthineers, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/somatom definition as 128 slice ct scanner/product/Siemens Healthineers
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    somatom definition as 128 slice ct scanner - by Bioz Stars, 2019-05
    77/100 stars
      Buy from Supplier

    Image Search Results


    The original lesions in right adrenal gland and false-positive images in various bone segments of 2 years and 9 months old girl (patient 1). a DWIBS (anterior). b DWIBS (lateral). c 123 I-MIBG scintigraphy/SPECT (anterior). d 123 I-MIBG scintigraphy/SPECT (lateral). The MIP of 18 F-FDG PET ( e ) and DWIBS ( f ). Long arrows show the original lesion ( a – f ). Arrowheads show false-positive images ( a, b, e, f )

    Journal: Annals of Nuclear Medicine

    Article Title: Diagnostic performance of 18F-FDG PET/CT and whole-body diffusion-weighted imaging with background body suppression (DWIBS) in detection of lymph node and bone metastases from pediatric neuroblastoma

    doi: 10.1007/s12149-018-1254-z

    Figure Lengend Snippet: The original lesions in right adrenal gland and false-positive images in various bone segments of 2 years and 9 months old girl (patient 1). a DWIBS (anterior). b DWIBS (lateral). c 123 I-MIBG scintigraphy/SPECT (anterior). d 123 I-MIBG scintigraphy/SPECT (lateral). The MIP of 18 F-FDG PET ( e ) and DWIBS ( f ). Long arrows show the original lesion ( a – f ). Arrowheads show false-positive images ( a, b, e, f )

    Article Snippet: 18 F-FDG PET/CT examination was performed with a PET/CT scanner (Biograph 16; Siemens Healthcare, Erlangen, Germany).

    Techniques: Single Photon Emission Computed Tomography, Positron Emission Tomography

    The original lesion in left adrenal gland and false-positive findings in various bone segments of 2 years and 8 months old boy (patient 10). a DWIBS (anterior). b 123 I-MIBG scintigraphy/SPECT (anterior). The MIP of 18 F-FDG PET ( c ) and DWIBS ( d ). Long arrows show the original lesion ( a – d ). Arrowheads show false-positive images ( a, c, d )

    Journal: Annals of Nuclear Medicine

    Article Title: Diagnostic performance of 18F-FDG PET/CT and whole-body diffusion-weighted imaging with background body suppression (DWIBS) in detection of lymph node and bone metastases from pediatric neuroblastoma

    doi: 10.1007/s12149-018-1254-z

    Figure Lengend Snippet: The original lesion in left adrenal gland and false-positive findings in various bone segments of 2 years and 8 months old boy (patient 10). a DWIBS (anterior). b 123 I-MIBG scintigraphy/SPECT (anterior). The MIP of 18 F-FDG PET ( c ) and DWIBS ( d ). Long arrows show the original lesion ( a – d ). Arrowheads show false-positive images ( a, c, d )

    Article Snippet: 18 F-FDG PET/CT examination was performed with a PET/CT scanner (Biograph 16; Siemens Healthcare, Erlangen, Germany).

    Techniques: Single Photon Emission Computed Tomography, Positron Emission Tomography

    The original lesions in bilateral retroperitoneal regions, metastases in left supraclavicular lymph nodes, and false-positive images of skeletons of 1 year and 7 months old girl (patient 6). a 18 F-FDG PET/CT. b DWIBS. c 123 I-MIBG scintigraphy/SPECT-CT. d CT. The maximum intensity projection (MIP) of 18 F-FDG PET ( e ) and DWIBS ( f ). Long arrows show the original lesions ( e, f ). Short arrows show metastases ( a – d ). Arrowheads show false-positive images in thoracic spine, left rib and scapula ( b ) and in various bone segments ( e, f )

    Journal: Annals of Nuclear Medicine

    Article Title: Diagnostic performance of 18F-FDG PET/CT and whole-body diffusion-weighted imaging with background body suppression (DWIBS) in detection of lymph node and bone metastases from pediatric neuroblastoma

    doi: 10.1007/s12149-018-1254-z

    Figure Lengend Snippet: The original lesions in bilateral retroperitoneal regions, metastases in left supraclavicular lymph nodes, and false-positive images of skeletons of 1 year and 7 months old girl (patient 6). a 18 F-FDG PET/CT. b DWIBS. c 123 I-MIBG scintigraphy/SPECT-CT. d CT. The maximum intensity projection (MIP) of 18 F-FDG PET ( e ) and DWIBS ( f ). Long arrows show the original lesions ( e, f ). Short arrows show metastases ( a – d ). Arrowheads show false-positive images in thoracic spine, left rib and scapula ( b ) and in various bone segments ( e, f )

    Article Snippet: 18 F-FDG PET/CT examination was performed with a PET/CT scanner (Biograph 16; Siemens Healthcare, Erlangen, Germany).

    Techniques: Positron Emission Tomography, Single Photon Emission Computed Tomography

    Metastasis in left temporal bone of skull of 1 year and 7 months old girl (patient 6). a 18 F-FDG PET/CT. b DWIBS. c 123 I-MIBG scintigraphy/SPECT-CT. d Bone scintigraphy/SPECT. e CT. Arrows show metastasis ( a – e ). Arrowhead shows false-positive images in sphenoidal bone ( b )

    Journal: Annals of Nuclear Medicine

    Article Title: Diagnostic performance of 18F-FDG PET/CT and whole-body diffusion-weighted imaging with background body suppression (DWIBS) in detection of lymph node and bone metastases from pediatric neuroblastoma

    doi: 10.1007/s12149-018-1254-z

    Figure Lengend Snippet: Metastasis in left temporal bone of skull of 1 year and 7 months old girl (patient 6). a 18 F-FDG PET/CT. b DWIBS. c 123 I-MIBG scintigraphy/SPECT-CT. d Bone scintigraphy/SPECT. e CT. Arrows show metastasis ( a – e ). Arrowhead shows false-positive images in sphenoidal bone ( b )

    Article Snippet: 18 F-FDG PET/CT examination was performed with a PET/CT scanner (Biograph 16; Siemens Healthcare, Erlangen, Germany).

    Techniques: Positron Emission Tomography, Single Photon Emission Computed Tomography

    The original lesion in left adrenal gland and false-positive images of skeletons of 2 years and 2 months old boy (patient 8). a DWIBS. b 18 F-FDG PET/CT. c 123 I-MIBG scintigraphy/SPECT-CT. The MIP of 18 F-FDG PET ( d ) and DWIBS ( e ). Long arrows show the original lesion ( d, e ). Arrowheads show false-positive images in pelvic bones ( a ) and various bone segments ( d, e ). Short arrow shows 18 F-FDG uptake in right ureter ( b )

    Journal: Annals of Nuclear Medicine

    Article Title: Diagnostic performance of 18F-FDG PET/CT and whole-body diffusion-weighted imaging with background body suppression (DWIBS) in detection of lymph node and bone metastases from pediatric neuroblastoma

    doi: 10.1007/s12149-018-1254-z

    Figure Lengend Snippet: The original lesion in left adrenal gland and false-positive images of skeletons of 2 years and 2 months old boy (patient 8). a DWIBS. b 18 F-FDG PET/CT. c 123 I-MIBG scintigraphy/SPECT-CT. The MIP of 18 F-FDG PET ( d ) and DWIBS ( e ). Long arrows show the original lesion ( d, e ). Arrowheads show false-positive images in pelvic bones ( a ) and various bone segments ( d, e ). Short arrow shows 18 F-FDG uptake in right ureter ( b )

    Article Snippet: 18 F-FDG PET/CT examination was performed with a PET/CT scanner (Biograph 16; Siemens Healthcare, Erlangen, Germany).

    Techniques: Positron Emission Tomography, Single Photon Emission Computed Tomography

    The original lesions in left adrenal gland, metastases in paraaortic lymph nodes and thoracic and lumbar spines, and false-positive images of skeletons of 4 years and 9 months old girl (patient 11). a 18 F-FDG PET/CT. b DWIBS. c 123 I-MIBG scintigraphy/SPECT-CT. d CT. e Bone scintigraphy/SPECT. The MIP of 18 F-FDG PET ( f ) and DWIBS ( g ). Long arrows show the original lesion ( f, g ). Short arrows show lymph node metastases ( a – g ). Empty arrows show bone metastases ( a – c, e – g ). Arrowheads show false-positive images of various bone segments ( f, g )

    Journal: Annals of Nuclear Medicine

    Article Title: Diagnostic performance of 18F-FDG PET/CT and whole-body diffusion-weighted imaging with background body suppression (DWIBS) in detection of lymph node and bone metastases from pediatric neuroblastoma

    doi: 10.1007/s12149-018-1254-z

    Figure Lengend Snippet: The original lesions in left adrenal gland, metastases in paraaortic lymph nodes and thoracic and lumbar spines, and false-positive images of skeletons of 4 years and 9 months old girl (patient 11). a 18 F-FDG PET/CT. b DWIBS. c 123 I-MIBG scintigraphy/SPECT-CT. d CT. e Bone scintigraphy/SPECT. The MIP of 18 F-FDG PET ( f ) and DWIBS ( g ). Long arrows show the original lesion ( f, g ). Short arrows show lymph node metastases ( a – g ). Empty arrows show bone metastases ( a – c, e – g ). Arrowheads show false-positive images of various bone segments ( f, g )

    Article Snippet: 18 F-FDG PET/CT examination was performed with a PET/CT scanner (Biograph 16; Siemens Healthcare, Erlangen, Germany).

    Techniques: Positron Emission Tomography, Single Photon Emission Computed Tomography

    Maximum intensity projection of whole body (A) and PET/CT images (B) showed increased FDG uptake in the right C6, C7, C8, T1nerve roots and branchial plexus, and bilateral T2, T3 nerve roots

    Journal: Asia Oceania Journal of Nuclear Medicine and Biology

    Article Title: 18F-FDG PET/CT in Neurolymphomatosis: Report of 3 Cases

    doi:

    Figure Lengend Snippet: Maximum intensity projection of whole body (A) and PET/CT images (B) showed increased FDG uptake in the right C6, C7, C8, T1nerve roots and branchial plexus, and bilateral T2, T3 nerve roots

    Article Snippet: The whole-body and additional scans were performed at 60 minutes after 18 F-FDG injection in a PET/CT scanner (Biograph True D w/true V, Siemens Medical System).

    Techniques: Positron Emission Tomography

    Maximum intensity projection of whole body coronal (A) , sagital (B) , thoracic transaxial (C) , and PET/CT images (D, E) showed increased FDG uptake of the right cervicothoracic ganglion (long arrow), along the 7 th -10 th intercostal nerves, branches of the vagus nerve (blue arrows)

    Journal: Asia Oceania Journal of Nuclear Medicine and Biology

    Article Title: 18F-FDG PET/CT in Neurolymphomatosis: Report of 3 Cases

    doi:

    Figure Lengend Snippet: Maximum intensity projection of whole body coronal (A) , sagital (B) , thoracic transaxial (C) , and PET/CT images (D, E) showed increased FDG uptake of the right cervicothoracic ganglion (long arrow), along the 7 th -10 th intercostal nerves, branches of the vagus nerve (blue arrows)

    Article Snippet: The whole-body and additional scans were performed at 60 minutes after 18 F-FDG injection in a PET/CT scanner (Biograph True D w/true V, Siemens Medical System).

    Techniques: Positron Emission Tomography

    Maximum intensity projection of brain tranaxial (A) coronal (B) and sagital (C) ; PET/CT (D, G) and PET images (E) showed increased FDG uptake in bilateral cranial nerve V, Gasser’s ganglions, and complex of the right cranial nerve VII/VIII

    Journal: Asia Oceania Journal of Nuclear Medicine and Biology

    Article Title: 18F-FDG PET/CT in Neurolymphomatosis: Report of 3 Cases

    doi:

    Figure Lengend Snippet: Maximum intensity projection of brain tranaxial (A) coronal (B) and sagital (C) ; PET/CT (D, G) and PET images (E) showed increased FDG uptake in bilateral cranial nerve V, Gasser’s ganglions, and complex of the right cranial nerve VII/VIII

    Article Snippet: The whole-body and additional scans were performed at 60 minutes after 18 F-FDG injection in a PET/CT scanner (Biograph True D w/true V, Siemens Medical System).

    Techniques: Positron Emission Tomography

    Maximum intensity projection of brain coronal (A) and sagital (B) , and PET/CT images of brain (C-I) showed increased FDG uptake in the left cranial nerves: (A-E) Cranial nerves V and V3; (B, G) complex of cranial nerves III, IV, VI, V1, V2 at cavernous sinus (long arrows); (H) complex of cranial nerves IX, X, XI at jugular foramen; and (I) cranial nerve XII at hypoglossal canal

    Journal: Asia Oceania Journal of Nuclear Medicine and Biology

    Article Title: 18F-FDG PET/CT in Neurolymphomatosis: Report of 3 Cases

    doi:

    Figure Lengend Snippet: Maximum intensity projection of brain coronal (A) and sagital (B) , and PET/CT images of brain (C-I) showed increased FDG uptake in the left cranial nerves: (A-E) Cranial nerves V and V3; (B, G) complex of cranial nerves III, IV, VI, V1, V2 at cavernous sinus (long arrows); (H) complex of cranial nerves IX, X, XI at jugular foramen; and (I) cranial nerve XII at hypoglossal canal

    Article Snippet: The whole-body and additional scans were performed at 60 minutes after 18 F-FDG injection in a PET/CT scanner (Biograph True D w/true V, Siemens Medical System).

    Techniques: Positron Emission Tomography

    Maximum intensity projection of whole body (A) and PET/CT images (B-H) showed increased FDG uptake in the left C2 nerve root (B) ; C5-C6 spinal cord, C7 nerve root, brachial plexus (C,D) ; multiple focal lesions in liver (E) ; and bilateral lumbosacral plexuses and along the sciatic nerves (G,H)

    Journal: Asia Oceania Journal of Nuclear Medicine and Biology

    Article Title: 18F-FDG PET/CT in Neurolymphomatosis: Report of 3 Cases

    doi:

    Figure Lengend Snippet: Maximum intensity projection of whole body (A) and PET/CT images (B-H) showed increased FDG uptake in the left C2 nerve root (B) ; C5-C6 spinal cord, C7 nerve root, brachial plexus (C,D) ; multiple focal lesions in liver (E) ; and bilateral lumbosacral plexuses and along the sciatic nerves (G,H)

    Article Snippet: The whole-body and additional scans were performed at 60 minutes after 18 F-FDG injection in a PET/CT scanner (Biograph True D w/true V, Siemens Medical System).

    Techniques: Positron Emission Tomography

    Correlation of microarray and qPCR data from fresh vs. frozen tissue. Correlation of microarray and qPCR results based on data from RNA extracted from fresh mouse brains in the DA TC and DR versus the use of RNA extracted from flash frozen brains in the PbTx TC. While minor differences were observed depending on the data set used, one data set did not consistently yield higher correlations. It does not appear that the use of frozen tissue, rather than fresh, appreciably influences the observed correlations with qPCR data. Asterisks indicate a statistically significant correlation (p

    Journal: Biological Procedures Online

    Article Title: Microarray validation: factors influencing correlation between oligonucleotide microarrays and real-time PCR

    doi: 10.1251/bpo126

    Figure Lengend Snippet: Correlation of microarray and qPCR data from fresh vs. frozen tissue. Correlation of microarray and qPCR results based on data from RNA extracted from fresh mouse brains in the DA TC and DR versus the use of RNA extracted from flash frozen brains in the PbTx TC. While minor differences were observed depending on the data set used, one data set did not consistently yield higher correlations. It does not appear that the use of frozen tissue, rather than fresh, appreciably influences the observed correlations with qPCR data. Asterisks indicate a statistically significant correlation (p

    Article Snippet: Microarrays were imaged using an Agilent microarray scanner.

    Techniques: Microarray, Real-time Polymerase Chain Reaction

    Analysis of data correlation categorized by direction of regulation, spot intensity, and cycle threshold. Correlation of microarray and qPCR data as it relates to (A) direction of regulation, (B) Log (spot intensity), and (C) cycle threshold. Spot intensity data was binned by quartiles and thus, as the intensities from each experiment differed slightly, actual intensities are not indicated in the legend. Asterisks indicate a statistically significant correlation of array and qPCR data (p

    Journal: Biological Procedures Online

    Article Title: Microarray validation: factors influencing correlation between oligonucleotide microarrays and real-time PCR

    doi: 10.1251/bpo126

    Figure Lengend Snippet: Analysis of data correlation categorized by direction of regulation, spot intensity, and cycle threshold. Correlation of microarray and qPCR data as it relates to (A) direction of regulation, (B) Log (spot intensity), and (C) cycle threshold. Spot intensity data was binned by quartiles and thus, as the intensities from each experiment differed slightly, actual intensities are not indicated in the legend. Asterisks indicate a statistically significant correlation of array and qPCR data (p

    Article Snippet: Microarrays were imaged using an Agilent microarray scanner.

    Techniques: Microarray, Real-time Polymerase Chain Reaction

    Analysis of data correlation categorized by fold change. Correlation of microarray and qPCR data as it relates to (A) fold change measured by microarray and (B) fold change measured by qPCR. The combined data set of

    Journal: Biological Procedures Online

    Article Title: Microarray validation: factors influencing correlation between oligonucleotide microarrays and real-time PCR

    doi: 10.1251/bpo126

    Figure Lengend Snippet: Analysis of data correlation categorized by fold change. Correlation of microarray and qPCR data as it relates to (A) fold change measured by microarray and (B) fold change measured by qPCR. The combined data set of "all data" was queried first by microarray fold change (1.4 fold cut-off) and then by (C) spot intensity and (D) Ct values to determine the overall impact of fold change on array and qPCR data correlations. Asterisks indicate a statistically significant correlation (p

    Article Snippet: Microarrays were imaged using an Agilent microarray scanner.

    Techniques: Microarray, Real-time Polymerase Chain Reaction

    Analysis of data correlation categorized by p-values from microarray analyses. (A) Correlation of microarray and qPCR data as it relates to microarray spot p-values. The combined data set of

    Journal: Biological Procedures Online

    Article Title: Microarray validation: factors influencing correlation between oligonucleotide microarrays and real-time PCR

    doi: 10.1251/bpo126

    Figure Lengend Snippet: Analysis of data correlation categorized by p-values from microarray analyses. (A) Correlation of microarray and qPCR data as it relates to microarray spot p-values. The combined data set of "all data" was analyzed by (B) spot intensity and (C) Ct values to determine the effect of p-values (0.0001 cutoff) on array and qPCR data correlation. P-values are based on calculations including signal strength, background values, spot morphology, fold change, variation between replicates, etc. Asterisks indicate a statistically significant result (p

    Article Snippet: Microarrays were imaged using an Agilent microarray scanner.

    Techniques: Microarray, Real-time Polymerase Chain Reaction

    Combined effects of array fold change and p-value on data correlation. Correlation of microarray and qPCR data as it relates to both array fold change and p-value. Analyses of the combined data set of

    Journal: Biological Procedures Online

    Article Title: Microarray validation: factors influencing correlation between oligonucleotide microarrays and real-time PCR

    doi: 10.1251/bpo126

    Figure Lengend Snippet: Combined effects of array fold change and p-value on data correlation. Correlation of microarray and qPCR data as it relates to both array fold change and p-value. Analyses of the combined data set of "all data" indicate that fold change has the greatest impact on array and qPCR data correlation. However, array data quality, measured here as a p-value, is essential to predicting reliable data. Asterisks indicate a statistically significant correlation (p

    Article Snippet: Microarrays were imaged using an Agilent microarray scanner.

    Techniques: Microarray, Real-time Polymerase Chain Reaction

    Effects of composite array use on array and qPCR data correlation. Correlation of microarray and qPCR results based on data from individual animals versus the use of weighted averages from composite array data. While minor differences were observed depending on the data set used, one data set did not consistently yield higher correlations. It does not appear that the use of composite arrays appreciably influence the observed correlations with qPCR data. Asterisks indicate a statistically significant correlation (p

    Journal: Biological Procedures Online

    Article Title: Microarray validation: factors influencing correlation between oligonucleotide microarrays and real-time PCR

    doi: 10.1251/bpo126

    Figure Lengend Snippet: Effects of composite array use on array and qPCR data correlation. Correlation of microarray and qPCR results based on data from individual animals versus the use of weighted averages from composite array data. While minor differences were observed depending on the data set used, one data set did not consistently yield higher correlations. It does not appear that the use of composite arrays appreciably influence the observed correlations with qPCR data. Asterisks indicate a statistically significant correlation (p

    Article Snippet: Microarrays were imaged using an Agilent microarray scanner.

    Techniques: Real-time Polymerase Chain Reaction, Microarray

    Validation of Whole Genome Transposon Analysis Using Two Sequenced Strains of S. cerevisiae (A) Whole genome comparison of full-length Ty1 and Ty2 elements from yeast strains RM11 and S288c after hybridization to the same Agilent yeast whole genome microarray. Black circles indicate the position of Ty1 or Ty2 full-length elements annotated for S288c in SGD. Triangles indicate full-length Ty2 elements identified in the sequence of RM11. Red peaks correspond to potential Ty1 or Ty2 elements present in S288c, while green peaks correspond to potential Ty1 or Ty2 peaks present in RM11. (B) Comparison of location of Ty1 full-length elements (green) and Ty2 full-length elements present in S288c. Symbols are as above. Numbers above various peaks refer to the following: 1, false-negative S288c elements obscured by overlapping elements in RM11; 2, false-negative S288c elements located in regions that are poorly represented by features on the array; 3, false-negative S288c elements that missed the criteria for calling a peak; 4, unannotated Ty1 full-length elements in S288c confirmed in this study; 5, false-positive peaks due to borderline elevated differential hybridization; 6, false-positive peaks corresponding to non-Ty repetitive elements in the genome. Grey horizontal lines above and below the central line for each chromosome correspond to a 3-fold difference in normalized ratio of Cy5 and Cy3 signal intensity.

    Journal: PLoS Genetics

    Article Title: Global Mapping of Transposon Location

    doi: 10.1371/journal.pgen.0020212

    Figure Lengend Snippet: Validation of Whole Genome Transposon Analysis Using Two Sequenced Strains of S. cerevisiae (A) Whole genome comparison of full-length Ty1 and Ty2 elements from yeast strains RM11 and S288c after hybridization to the same Agilent yeast whole genome microarray. Black circles indicate the position of Ty1 or Ty2 full-length elements annotated for S288c in SGD. Triangles indicate full-length Ty2 elements identified in the sequence of RM11. Red peaks correspond to potential Ty1 or Ty2 elements present in S288c, while green peaks correspond to potential Ty1 or Ty2 peaks present in RM11. (B) Comparison of location of Ty1 full-length elements (green) and Ty2 full-length elements present in S288c. Symbols are as above. Numbers above various peaks refer to the following: 1, false-negative S288c elements obscured by overlapping elements in RM11; 2, false-negative S288c elements located in regions that are poorly represented by features on the array; 3, false-negative S288c elements that missed the criteria for calling a peak; 4, unannotated Ty1 full-length elements in S288c confirmed in this study; 5, false-positive peaks due to borderline elevated differential hybridization; 6, false-positive peaks corresponding to non-Ty repetitive elements in the genome. Grey horizontal lines above and below the central line for each chromosome correspond to a 3-fold difference in normalized ratio of Cy5 and Cy3 signal intensity.

    Article Snippet: Hybridization was carried out at 60 °C for 17 h. Slides were washed according to the Agilent SSPE Wash protocol, dried in acetonitrile, and then scanned using an Agilent Microarray Scanner.

    Techniques: Hybridization, Microarray, Sequencing

    A General Schematic Diagram of the Steps Involved in Extracting, Labeling, and Identifying the Position of Transposons within a Genome Step 1. Genomic DNA is digested with a restriction endonuclease containing a cut site (triangle) within the transposon (red box). This results in multiple restriction fragments, including ones containing transposon and contiguous flanking DNA. Step 2. Digested DNA (which may be pooled from multiple separate digests) is mixed with oligonucleotide probes that have been designed to anneal to specific sequences within the transposon. Separate probe sets anneal to different transposons (as shown), or separate genomic DNA samples are used to compare transposon content from different sources. Step 3. After heat denaturation and reannealing, the mixture is incubated in the presence of a DNA polymerase and dNTPs, one of which is biotinylated (stick with star atop it). This allows specific extension from the annealed 3′ probe termini. Step 4. Extended probes and their annealed templates are purified away from the mixture using magnetic streptavidin-coated beads (star labeled with Fe +3 ). Step 5. The extracted templates are released by heating. Step 6. The templates are labeled using Cy3- or Cy5-labeled nucleotides (green and red lollipops, respectively) in the presence of random primers and a DNA polymerase. Step 7. Differentially labeled DNAs are hybridized to a microarray slide with features distributed throughout the genome. After washing, the array is scanned to identify features (circles) that are common to both DNA sources (yellow circles) or that have been differentially extracted (green or red circles). Step 8. The log 2 ratio of signal intensity for the two dyes is quantitated and graphically represented along each chromosome to identify contiguous segments of differential signal that correspond to the DNA flanking the original transposons.

    Journal: PLoS Genetics

    Article Title: Global Mapping of Transposon Location

    doi: 10.1371/journal.pgen.0020212

    Figure Lengend Snippet: A General Schematic Diagram of the Steps Involved in Extracting, Labeling, and Identifying the Position of Transposons within a Genome Step 1. Genomic DNA is digested with a restriction endonuclease containing a cut site (triangle) within the transposon (red box). This results in multiple restriction fragments, including ones containing transposon and contiguous flanking DNA. Step 2. Digested DNA (which may be pooled from multiple separate digests) is mixed with oligonucleotide probes that have been designed to anneal to specific sequences within the transposon. Separate probe sets anneal to different transposons (as shown), or separate genomic DNA samples are used to compare transposon content from different sources. Step 3. After heat denaturation and reannealing, the mixture is incubated in the presence of a DNA polymerase and dNTPs, one of which is biotinylated (stick with star atop it). This allows specific extension from the annealed 3′ probe termini. Step 4. Extended probes and their annealed templates are purified away from the mixture using magnetic streptavidin-coated beads (star labeled with Fe +3 ). Step 5. The extracted templates are released by heating. Step 6. The templates are labeled using Cy3- or Cy5-labeled nucleotides (green and red lollipops, respectively) in the presence of random primers and a DNA polymerase. Step 7. Differentially labeled DNAs are hybridized to a microarray slide with features distributed throughout the genome. After washing, the array is scanned to identify features (circles) that are common to both DNA sources (yellow circles) or that have been differentially extracted (green or red circles). Step 8. The log 2 ratio of signal intensity for the two dyes is quantitated and graphically represented along each chromosome to identify contiguous segments of differential signal that correspond to the DNA flanking the original transposons.

    Article Snippet: Hybridization was carried out at 60 °C for 17 h. Slides were washed according to the Agilent SSPE Wash protocol, dried in acetonitrile, and then scanned using an Agilent Microarray Scanner.

    Techniques: Labeling, Incubation, Purification, Microarray

    Identifying a Unique Ty1 Element in Otherwise Isogenic Strains (A) Two isogenic yeast strains (FY5 and FY2) differ only by the presence of a Ty1 insertion in Chromosome V within the URA3 gene in FY2. After labeling transposon extracted DNA from FY2 with Cy3 (green) and transposon extracted DNA from FY5 with Cy5 (red), the labeled DNA was hybridized to an Agilent yeast whole genome microarray with > 40,000 unique features (yeast repetitive DNA was avoided during array construction). Log 2 ratio of hybridization for each feature along each chromosome is shown plotted in genome order using the TreeView Karyoscope function. The one region of significant differential hybridization is marked with an arrow. The grey horizontal lines above and below each chromosome correspond to 3-fold differential hybridization intensity. (B) Zoom view of a portion of Chromosome V and the peak of differential hybridization corresponding to the ∼8 kb surrounding URA3 (red box). The positions of nearby restriction sites for the enzymes used initially to digest genomic DNA are shown based on a GBrowse view of the region from SGD.

    Journal: PLoS Genetics

    Article Title: Global Mapping of Transposon Location

    doi: 10.1371/journal.pgen.0020212

    Figure Lengend Snippet: Identifying a Unique Ty1 Element in Otherwise Isogenic Strains (A) Two isogenic yeast strains (FY5 and FY2) differ only by the presence of a Ty1 insertion in Chromosome V within the URA3 gene in FY2. After labeling transposon extracted DNA from FY2 with Cy3 (green) and transposon extracted DNA from FY5 with Cy5 (red), the labeled DNA was hybridized to an Agilent yeast whole genome microarray with > 40,000 unique features (yeast repetitive DNA was avoided during array construction). Log 2 ratio of hybridization for each feature along each chromosome is shown plotted in genome order using the TreeView Karyoscope function. The one region of significant differential hybridization is marked with an arrow. The grey horizontal lines above and below each chromosome correspond to 3-fold differential hybridization intensity. (B) Zoom view of a portion of Chromosome V and the peak of differential hybridization corresponding to the ∼8 kb surrounding URA3 (red box). The positions of nearby restriction sites for the enzymes used initially to digest genomic DNA are shown based on a GBrowse view of the region from SGD.

    Article Snippet: Hybridization was carried out at 60 °C for 17 h. Slides were washed according to the Agilent SSPE Wash protocol, dried in acetonitrile, and then scanned using an Agilent Microarray Scanner.

    Techniques: Labeling, Microarray, Hybridization

    Transposon Map of SK1 The positions of Ty1 and Ty2 full-length elements and Ty3 LTR elements in strain SK1 are shown, based on Agilent yeast whole genome microarray (Ty1 and Ty2) and Agilent ORF array (Ty3 LTR) analysis of this uncharacterized genome.

    Journal: PLoS Genetics

    Article Title: Global Mapping of Transposon Location

    doi: 10.1371/journal.pgen.0020212

    Figure Lengend Snippet: Transposon Map of SK1 The positions of Ty1 and Ty2 full-length elements and Ty3 LTR elements in strain SK1 are shown, based on Agilent yeast whole genome microarray (Ty1 and Ty2) and Agilent ORF array (Ty3 LTR) analysis of this uncharacterized genome.

    Article Snippet: Hybridization was carried out at 60 °C for 17 h. Slides were washed according to the Agilent SSPE Wash protocol, dried in acetonitrile, and then scanned using an Agilent Microarray Scanner.

    Techniques: Microarray

    Analysis of Modified Bacterial (“Artificial”) Transposon Insertions in the S. cerevisiae Genome (A) Positions of five independent pooled artificial transposons from a yeast insertion library were determined after extracting StuI-digested yeast genomic DNA with probes designed to correspond to either strand at the 5′ or 3′ end of URA3, labeling with Cy3 and Cy5, respectively, and hybridizing to an Agilent yeast whole genome microarray. Arrows signify locations of significant differential hybridization. “URA3” indicates the actual URA3 locus on Chromosome V. The asterisk indicates an insertion on Chromosome XVI in which only the flanking region 3′ to the transposon is detected. Vertical lines above and below the horizontal for each chromosome represent the log 2 ratio of hybridization intensity for Cy5 versus Cy3 at each feature along the Agilent yeast whole genome microarray. For each insertion, the actual insertion site, determined by sequencing, and the position of the first significant flanking features are as follows: Chromosome IV, 368020, and 367656–367715 and 368589–368648; Chromosome IX, 55576, and 55291–55350 and 55808–55867; Chromosome XI, 613654, and 612706–612765 and 614005–614064; Chromosome XII, 387226, and 386346–386405 and 387248–387307; and Chromosome XVI, 296609, and 296350–296409 to 297592–297651. (B) An enlargement of the region detected on Chromosome XI, showing the structure of the artificial transposon, its unique StuI site, the bases covered by the oligonucleotides in the features on either side of the transition from significant differential Cy5 labeling to Cy3 labeling, and the position of the actual insertion. The map of the region from GBrowse of SGD shows the position of StuI restriction sites in the region. Grey horizontal lines above and below the central line for each chromosome correspond to a 3-fold ratio of signal intensity. Note that the transposon inserted in opposite orientation relative to the chromosome numbering, and is therefore flipped in the figure.

    Journal: PLoS Genetics

    Article Title: Global Mapping of Transposon Location

    doi: 10.1371/journal.pgen.0020212

    Figure Lengend Snippet: Analysis of Modified Bacterial (“Artificial”) Transposon Insertions in the S. cerevisiae Genome (A) Positions of five independent pooled artificial transposons from a yeast insertion library were determined after extracting StuI-digested yeast genomic DNA with probes designed to correspond to either strand at the 5′ or 3′ end of URA3, labeling with Cy3 and Cy5, respectively, and hybridizing to an Agilent yeast whole genome microarray. Arrows signify locations of significant differential hybridization. “URA3” indicates the actual URA3 locus on Chromosome V. The asterisk indicates an insertion on Chromosome XVI in which only the flanking region 3′ to the transposon is detected. Vertical lines above and below the horizontal for each chromosome represent the log 2 ratio of hybridization intensity for Cy5 versus Cy3 at each feature along the Agilent yeast whole genome microarray. For each insertion, the actual insertion site, determined by sequencing, and the position of the first significant flanking features are as follows: Chromosome IV, 368020, and 367656–367715 and 368589–368648; Chromosome IX, 55576, and 55291–55350 and 55808–55867; Chromosome XI, 613654, and 612706–612765 and 614005–614064; Chromosome XII, 387226, and 386346–386405 and 387248–387307; and Chromosome XVI, 296609, and 296350–296409 to 297592–297651. (B) An enlargement of the region detected on Chromosome XI, showing the structure of the artificial transposon, its unique StuI site, the bases covered by the oligonucleotides in the features on either side of the transition from significant differential Cy5 labeling to Cy3 labeling, and the position of the actual insertion. The map of the region from GBrowse of SGD shows the position of StuI restriction sites in the region. Grey horizontal lines above and below the central line for each chromosome correspond to a 3-fold ratio of signal intensity. Note that the transposon inserted in opposite orientation relative to the chromosome numbering, and is therefore flipped in the figure.

    Article Snippet: Hybridization was carried out at 60 °C for 17 h. Slides were washed according to the Agilent SSPE Wash protocol, dried in acetonitrile, and then scanned using an Agilent Microarray Scanner.

    Techniques: Modification, Labeling, Microarray, Hybridization, Sequencing

    A comparison of the CPS locus sequence between serotype 6B variant and wild type. These variants were initially detected by microarray as having atypical CPS genes and were further analysed by whole-genome sequencing. A comparative analysis of the CPS locus sequences between variants of serotype 6B and reference sequence CR931639 was performed using Artemis. The divergent gene(s) for the variant are highlighted in red. The 6B variant [ERS096169] harboured an intact allele of the licD- phosphotransferase gene, while a 297 bp deletion was observed in the reference

    Journal: BMC Infectious Diseases

    Article Title: High multiple carriage and emergence of Streptococcus pneumoniae vaccine serotype variants in Malawian children

    doi: 10.1186/s12879-015-0980-2

    Figure Lengend Snippet: A comparison of the CPS locus sequence between serotype 6B variant and wild type. These variants were initially detected by microarray as having atypical CPS genes and were further analysed by whole-genome sequencing. A comparative analysis of the CPS locus sequences between variants of serotype 6B and reference sequence CR931639 was performed using Artemis. The divergent gene(s) for the variant are highlighted in red. The 6B variant [ERS096169] harboured an intact allele of the licD- phosphotransferase gene, while a 297 bp deletion was observed in the reference

    Article Snippet: The processed microarray slides were scanned using a high-resolution microarray scanner (Agilent, UK).

    Techniques: Sequencing, Variant Assay, Microarray

    A comparison of the CPS locus sequence between serotype 19A variant and wild type. These variants were initially detected by microarray as having unusual CPS genes and were further analysed by whole-genome sequencing. The CPS locus sequences were compared between variants of serotype 19A and reference sequences CR931675. The divergent gene(s) for the variant are highlighted in red. The CPS loci of serotype 19A variant [ERS096157] showed an inversion in rmlD gene

    Journal: BMC Infectious Diseases

    Article Title: High multiple carriage and emergence of Streptococcus pneumoniae vaccine serotype variants in Malawian children

    doi: 10.1186/s12879-015-0980-2

    Figure Lengend Snippet: A comparison of the CPS locus sequence between serotype 19A variant and wild type. These variants were initially detected by microarray as having unusual CPS genes and were further analysed by whole-genome sequencing. The CPS locus sequences were compared between variants of serotype 19A and reference sequences CR931675. The divergent gene(s) for the variant are highlighted in red. The CPS loci of serotype 19A variant [ERS096157] showed an inversion in rmlD gene

    Article Snippet: The processed microarray slides were scanned using a high-resolution microarray scanner (Agilent, UK).

    Techniques: Sequencing, Variant Assay, Microarray

    A comparison of the CPS locus sequence between serotype 20 variant and wild type. These variants were initially detected by microarray as having unusual CPS genes and were further analysed by whole-genome sequencing. A comparative analysis of the CPS locus sequences between variants of serotype 20 and reference sequences CR931679 was performed using EasyFig [ 47 ]. The divergent gene(s) for the variant are highlighted in red. The serotype 20 variant [ERS096158] contained a 717 base pair gene deletion within the whaF gene compared to the reference (CR931679)

    Journal: BMC Infectious Diseases

    Article Title: High multiple carriage and emergence of Streptococcus pneumoniae vaccine serotype variants in Malawian children

    doi: 10.1186/s12879-015-0980-2

    Figure Lengend Snippet: A comparison of the CPS locus sequence between serotype 20 variant and wild type. These variants were initially detected by microarray as having unusual CPS genes and were further analysed by whole-genome sequencing. A comparative analysis of the CPS locus sequences between variants of serotype 20 and reference sequences CR931679 was performed using EasyFig [ 47 ]. The divergent gene(s) for the variant are highlighted in red. The serotype 20 variant [ERS096158] contained a 717 base pair gene deletion within the whaF gene compared to the reference (CR931679)

    Article Snippet: The processed microarray slides were scanned using a high-resolution microarray scanner (Agilent, UK).

    Techniques: Sequencing, Variant Assay, Microarray

    Serotype-specific pneumococcal carriage in Malawian children, determined by microarray. A total of 179 pneumococcal strains were detected from 116 nasopharyngeal swabs, comprising 43 distinct pneumococcal serotypes and non-typeable strains (NT). The blue and red bar graphs represent serotypes detected in HIV positive and HIV negative children respectively. The serotypes were classified as Vaccine Type and Non-Vaccine Type. The non-vaccine serotypes were subdivided into high (Non-Vaccine Type (*)) and low (Non-Vaccine Type (**)) invasive potential based on the global frequency of isolation from invasive disease [ 24 ]. Individuals carrying multiple serotypes are represented more than once. The line graph represents cumulative frequency of serotypes isolated and was used to estimate the proportion of Vaccine Type (60 %) and Non-Vaccine Type (40 %)

    Journal: BMC Infectious Diseases

    Article Title: High multiple carriage and emergence of Streptococcus pneumoniae vaccine serotype variants in Malawian children

    doi: 10.1186/s12879-015-0980-2

    Figure Lengend Snippet: Serotype-specific pneumococcal carriage in Malawian children, determined by microarray. A total of 179 pneumococcal strains were detected from 116 nasopharyngeal swabs, comprising 43 distinct pneumococcal serotypes and non-typeable strains (NT). The blue and red bar graphs represent serotypes detected in HIV positive and HIV negative children respectively. The serotypes were classified as Vaccine Type and Non-Vaccine Type. The non-vaccine serotypes were subdivided into high (Non-Vaccine Type (*)) and low (Non-Vaccine Type (**)) invasive potential based on the global frequency of isolation from invasive disease [ 24 ]. Individuals carrying multiple serotypes are represented more than once. The line graph represents cumulative frequency of serotypes isolated and was used to estimate the proportion of Vaccine Type (60 %) and Non-Vaccine Type (40 %)

    Article Snippet: The processed microarray slides were scanned using a high-resolution microarray scanner (Agilent, UK).

    Techniques: Microarray, Isolation

    Multiple carriage of S. pneumoniae serotypes in Malawian children. Microarray was used to determine carriage of multiple pneumococcal serotypes in the nasopharynx of Malawian children. The overall frequency of multiple serotype carriage was 40 % (46/116), with co-colonising samples expressing two (27 %, 31/116)), three (11 %, 13/116) or four (2 %, 2/116) capsular types

    Journal: BMC Infectious Diseases

    Article Title: High multiple carriage and emergence of Streptococcus pneumoniae vaccine serotype variants in Malawian children

    doi: 10.1186/s12879-015-0980-2

    Figure Lengend Snippet: Multiple carriage of S. pneumoniae serotypes in Malawian children. Microarray was used to determine carriage of multiple pneumococcal serotypes in the nasopharynx of Malawian children. The overall frequency of multiple serotype carriage was 40 % (46/116), with co-colonising samples expressing two (27 %, 31/116)), three (11 %, 13/116) or four (2 %, 2/116) capsular types

    Article Snippet: The processed microarray slides were scanned using a high-resolution microarray scanner (Agilent, UK).

    Techniques: Microarray, Expressing

    Reactivity of sera from experimentally infected mallards against recombinant HA in ELISA and IVPM. Infection and sample collection scheme for experimentally inoculated mallards ( A ). Absolute AUC values ( B ) and fold induction ( C ) over the AUC of naive sera against recombinant HA in ELISA are shown, calculated for each HA. ( D ) AUC and ( E ) fold induction data collected via the IVPM. ( F ) Reactivity of sera to chimeric H5/3 (cH5/3), H3 and H5 in ELISA. area under the curve, AUC; enzyme-linked immunosorbent assay, ELISA; hemagglutinin, HA; influenza virus protein microarray, IVPM.

    Journal: Emerging Microbes & Infections

    Article Title: Development of an influenza virus protein microarray to measure the humoral response to influenza virus infection in mallards

    doi: 10.1038/emi.2017.98

    Figure Lengend Snippet: Reactivity of sera from experimentally infected mallards against recombinant HA in ELISA and IVPM. Infection and sample collection scheme for experimentally inoculated mallards ( A ). Absolute AUC values ( B ) and fold induction ( C ) over the AUC of naive sera against recombinant HA in ELISA are shown, calculated for each HA. ( D ) AUC and ( E ) fold induction data collected via the IVPM. ( F ) Reactivity of sera to chimeric H5/3 (cH5/3), H3 and H5 in ELISA. area under the curve, AUC; enzyme-linked immunosorbent assay, ELISA; hemagglutinin, HA; influenza virus protein microarray, IVPM.

    Article Snippet: Slides were allowed to dry at room temperature, and analyzed for mean fluorescence using a Vidia microarray scanner (Indevr, Boulder, CO, USA), using an exposure time of 1300 ms. AUC was measured as total peak area above the mean fluorescence of spots of the same HA incubated with naive mallard sera.

    Techniques: Infection, Recombinant, Hemagglutination Assay, Enzyme-linked Immunosorbent Assay, Microarray

    Correlation analysis of reactivity data measured by IVPM and ELISA. Correlation between IVPM and ELISA fold induction over naive sera ( A ) and IVPM and ELISA absolute AUC values ( B ) are shown, by HA. Correlation of ELISA and IVPM fold induction data for recombinant H3 shown as an example of the correlation analysis ( C ). area under the curve, AUC; enzyme-linked immunosorbent assay, ELISA; hemagglutinin, HA; influenza virus protein microarray, IVPM.

    Journal: Emerging Microbes & Infections

    Article Title: Development of an influenza virus protein microarray to measure the humoral response to influenza virus infection in mallards

    doi: 10.1038/emi.2017.98

    Figure Lengend Snippet: Correlation analysis of reactivity data measured by IVPM and ELISA. Correlation between IVPM and ELISA fold induction over naive sera ( A ) and IVPM and ELISA absolute AUC values ( B ) are shown, by HA. Correlation of ELISA and IVPM fold induction data for recombinant H3 shown as an example of the correlation analysis ( C ). area under the curve, AUC; enzyme-linked immunosorbent assay, ELISA; hemagglutinin, HA; influenza virus protein microarray, IVPM.

    Article Snippet: Slides were allowed to dry at room temperature, and analyzed for mean fluorescence using a Vidia microarray scanner (Indevr, Boulder, CO, USA), using an exposure time of 1300 ms. AUC was measured as total peak area above the mean fluorescence of spots of the same HA incubated with naive mallard sera.

    Techniques: Enzyme-linked Immunosorbent Assay, Hemagglutination Assay, Recombinant, Microarray

    Influenza virus protein microarray pipeline. Recombinant HA is expressed in a baculovirus expression system, purified, characterized and undergoes quality control ( A ). HAs are arrayed onto an epoxysilane-coated glass slide (Schott) using a Versa 110 spotter (Aurora Biomed) ( B ). The HAs are covalently bound to the slide and blocked; the arrays are incubated with sera, followed by fluorescently labeled secondary antibodies in a 96-well microarray gasket (Arrayit) ( C ). The arrays are then imaged ( D ), spots are automatically detected and their fluorescence is measured ( E ). Data from microarray imaging are analyzed in GraphPad Prism 7.0 ( F ). HA, hemagglutinin.

    Journal: Emerging Microbes & Infections

    Article Title: Development of an influenza virus protein microarray to measure the humoral response to influenza virus infection in mallards

    doi: 10.1038/emi.2017.98

    Figure Lengend Snippet: Influenza virus protein microarray pipeline. Recombinant HA is expressed in a baculovirus expression system, purified, characterized and undergoes quality control ( A ). HAs are arrayed onto an epoxysilane-coated glass slide (Schott) using a Versa 110 spotter (Aurora Biomed) ( B ). The HAs are covalently bound to the slide and blocked; the arrays are incubated with sera, followed by fluorescently labeled secondary antibodies in a 96-well microarray gasket (Arrayit) ( C ). The arrays are then imaged ( D ), spots are automatically detected and their fluorescence is measured ( E ). Data from microarray imaging are analyzed in GraphPad Prism 7.0 ( F ). HA, hemagglutinin.

    Article Snippet: Slides were allowed to dry at room temperature, and analyzed for mean fluorescence using a Vidia microarray scanner (Indevr, Boulder, CO, USA), using an exposure time of 1300 ms. AUC was measured as total peak area above the mean fluorescence of spots of the same HA incubated with naive mallard sera.

    Techniques: Microarray, Recombinant, Hemagglutination Assay, Expressing, Purification, Incubation, Labeling, Fluorescence, Imaging

    Establishing ELISA and IVPM for mallards. ELISA-IVPM correlation for serial 1:2 dilutions of mAb KB2, reacting to a conformational epitope on NC99 ( A ) and PR8 ( B ) H1 HA. The PCC and its P -value are indicated in both panels. ( C ) Testing of commercial secondary antibodies. Antibodies purchased from Antibodies Online (AbO), KPL (KPL) and Novus Biologicals (Novus) are shown, reacting against serum from a mallard infected with H3N8 and H6N2, binding H3, H6 and H18 HAs in ELISA. ( D ) Reactivity of different concentrations of pooled sera from mallards infected with H4N5 probed with different concentrations of the AbO secondary antibody in IVPM. The arrayed protein is recombinant H4. enzyme-linked immunosorbent assay, ELISA; influenza virus protein microarray, IVPM; Pearson correlation coefficients, PCC.

    Journal: Emerging Microbes & Infections

    Article Title: Development of an influenza virus protein microarray to measure the humoral response to influenza virus infection in mallards

    doi: 10.1038/emi.2017.98

    Figure Lengend Snippet: Establishing ELISA and IVPM for mallards. ELISA-IVPM correlation for serial 1:2 dilutions of mAb KB2, reacting to a conformational epitope on NC99 ( A ) and PR8 ( B ) H1 HA. The PCC and its P -value are indicated in both panels. ( C ) Testing of commercial secondary antibodies. Antibodies purchased from Antibodies Online (AbO), KPL (KPL) and Novus Biologicals (Novus) are shown, reacting against serum from a mallard infected with H3N8 and H6N2, binding H3, H6 and H18 HAs in ELISA. ( D ) Reactivity of different concentrations of pooled sera from mallards infected with H4N5 probed with different concentrations of the AbO secondary antibody in IVPM. The arrayed protein is recombinant H4. enzyme-linked immunosorbent assay, ELISA; influenza virus protein microarray, IVPM; Pearson correlation coefficients, PCC.

    Article Snippet: Slides were allowed to dry at room temperature, and analyzed for mean fluorescence using a Vidia microarray scanner (Indevr, Boulder, CO, USA), using an exposure time of 1300 ms. AUC was measured as total peak area above the mean fluorescence of spots of the same HA incubated with naive mallard sera.

    Techniques: Enzyme-linked Immunosorbent Assay, Hemagglutination Assay, Periodic Counter-current Chromatography, Infection, Binding Assay, Recombinant, Microarray

    Characterization of Hb following preterm rabbit pup IVH . The presence of Hb following IVH was characterized within the brain utilizing the inherent peroxidase activity of Hb. Rabbit pups with IVH or sham controls were euthanized at 72 h of age followed by saline and freshly prepared 4% PFA perfusion. Afterwards the brains were dissected out from the skulls and immersed in 4% PFA. Brains were prepared and sections and a number of subventricular and periventricular anatomically comparable regions of interests (ROI) were chosen. Neuroanatomically defined ROI's located in the rostral forebrain at the levels of rostral forebrain (Level 1), caudal forebrain (Level 2), rostral midbrain (Level 3) and caudal midbrain (Level 4), were stained for peroxidase activity of Hb as described in the Materials and Methods Section. Microscope analyses were performed on a wide-field Olympus microscope (IX73) and slide scanning were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730. Scanning was performed with a 40 × magnification lens. Images used for illustrations, from regions of interest (ROI), were grabbed with the viewer software NDP.view2 Viewing software. Scale bar of slide scan image indicate 2.5 mm and of ROI images indicate 500 μm.

    Journal: Frontiers in Physiology

    Article Title: High Presence of Extracellular Hemoglobin in the Periventricular White Matter Following Preterm Intraventricular Hemorrhage

    doi: 10.3389/fphys.2016.00330

    Figure Lengend Snippet: Characterization of Hb following preterm rabbit pup IVH . The presence of Hb following IVH was characterized within the brain utilizing the inherent peroxidase activity of Hb. Rabbit pups with IVH or sham controls were euthanized at 72 h of age followed by saline and freshly prepared 4% PFA perfusion. Afterwards the brains were dissected out from the skulls and immersed in 4% PFA. Brains were prepared and sections and a number of subventricular and periventricular anatomically comparable regions of interests (ROI) were chosen. Neuroanatomically defined ROI's located in the rostral forebrain at the levels of rostral forebrain (Level 1), caudal forebrain (Level 2), rostral midbrain (Level 3) and caudal midbrain (Level 4), were stained for peroxidase activity of Hb as described in the Materials and Methods Section. Microscope analyses were performed on a wide-field Olympus microscope (IX73) and slide scanning were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730. Scanning was performed with a 40 × magnification lens. Images used for illustrations, from regions of interest (ROI), were grabbed with the viewer software NDP.view2 Viewing software. Scale bar of slide scan image indicate 2.5 mm and of ROI images indicate 500 μm.

    Article Snippet: Slide scanning, used for overviews and some of the detailed histological and histochemical descriptions, were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730.

    Techniques: Activity Assay, Staining, Microscopy, Software

    Extracellular Hb is present within plastic areas of the periventricular white matter following preterm rabbit pup IVH . The relation between Hb  (B) , displayed in red and PSA-NCAM  (C) , displayed in green, a marker of areas with high plasticity, characterized by double labeling of selected regions of interests (ROI) of peroxidase activity corresponding areas  (A) , recorded in parallel sections. Rabbit pups with IVH or sham controls were euthanized at 72 h of age followed by saline and freshly prepared 4% PFA perfusion. Afterwards the brains were dissected from the skulls and immersed in 4% PFA. Brains were prepared and sections at the levels of rostral midbrain (Level 3) and caudal midbrain (Level 4), were immunolabeled for Hb and PSA-NCAM as described in the Materials and Methods section. DAPI labeled cell nuclei, displayed in blue, are shown in  (D)  and the merge of Hb, PSA-NCAM and DAPI is displayed in  (E) . Fluorescence microscope analyses were performed on a wide-field Olympus microscope (IX73) and slide scanning were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730. Scanning was performed with a 40 × magnification lens. Images used for illustrations, from ROI's, were grabbed with the viewer software NDP.view2 Viewing software. Scale bar of slide scan images indicate 500 μm and of ROI images indicate 25 μm.

    Journal: Frontiers in Physiology

    Article Title: High Presence of Extracellular Hemoglobin in the Periventricular White Matter Following Preterm Intraventricular Hemorrhage

    doi: 10.3389/fphys.2016.00330

    Figure Lengend Snippet: Extracellular Hb is present within plastic areas of the periventricular white matter following preterm rabbit pup IVH . The relation between Hb (B) , displayed in red and PSA-NCAM (C) , displayed in green, a marker of areas with high plasticity, characterized by double labeling of selected regions of interests (ROI) of peroxidase activity corresponding areas (A) , recorded in parallel sections. Rabbit pups with IVH or sham controls were euthanized at 72 h of age followed by saline and freshly prepared 4% PFA perfusion. Afterwards the brains were dissected from the skulls and immersed in 4% PFA. Brains were prepared and sections at the levels of rostral midbrain (Level 3) and caudal midbrain (Level 4), were immunolabeled for Hb and PSA-NCAM as described in the Materials and Methods section. DAPI labeled cell nuclei, displayed in blue, are shown in (D) and the merge of Hb, PSA-NCAM and DAPI is displayed in (E) . Fluorescence microscope analyses were performed on a wide-field Olympus microscope (IX73) and slide scanning were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730. Scanning was performed with a 40 × magnification lens. Images used for illustrations, from ROI's, were grabbed with the viewer software NDP.view2 Viewing software. Scale bar of slide scan images indicate 500 μm and of ROI images indicate 25 μm.

    Article Snippet: Slide scanning, used for overviews and some of the detailed histological and histochemical descriptions, were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730.

    Techniques: Marker, Labeling, Activity Assay, Immunolabeling, Fluorescence, Microscopy, Software

    Hemorrhage distribution following preterm rabbit pup IVH . Following IVH in preterm rabbit pups, the distribution of the hemorrhage was investigated. Rabbit pups with IVH or sham controls were euthanized at 72 h of age followed by saline and freshly prepared 4% PFA perfusion. Afterwards the brains were dissected out from the skulls and immersed in 4% PFA. Brains were prepared and sections from Control and IVH animals, at the levels of rostral forebrain (Level 1), caudal forebrain (Level 2), rostral midbrain (Level 3) and caudal midbrain (Level 4), were stained for hematoxylin and eosin (HE) and peroxidase activity of Hb (Peroxidase) as described in the Materials and Methods Section. Microscope analyses were performed on a wide-field Olympus microscope (IX73) and slide scanning were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730. Scanning was performed with a 40 × magnification lens. Scale bar indicate 2.5 mm and is applicable for all images.

    Journal: Frontiers in Physiology

    Article Title: High Presence of Extracellular Hemoglobin in the Periventricular White Matter Following Preterm Intraventricular Hemorrhage

    doi: 10.3389/fphys.2016.00330

    Figure Lengend Snippet: Hemorrhage distribution following preterm rabbit pup IVH . Following IVH in preterm rabbit pups, the distribution of the hemorrhage was investigated. Rabbit pups with IVH or sham controls were euthanized at 72 h of age followed by saline and freshly prepared 4% PFA perfusion. Afterwards the brains were dissected out from the skulls and immersed in 4% PFA. Brains were prepared and sections from Control and IVH animals, at the levels of rostral forebrain (Level 1), caudal forebrain (Level 2), rostral midbrain (Level 3) and caudal midbrain (Level 4), were stained for hematoxylin and eosin (HE) and peroxidase activity of Hb (Peroxidase) as described in the Materials and Methods Section. Microscope analyses were performed on a wide-field Olympus microscope (IX73) and slide scanning were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730. Scanning was performed with a 40 × magnification lens. Scale bar indicate 2.5 mm and is applicable for all images.

    Article Snippet: Slide scanning, used for overviews and some of the detailed histological and histochemical descriptions, were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730.

    Techniques: Staining, Activity Assay, Microscopy

    Presence of intracellular and extracellular Hb . The presence of RBCs and Hb following IVH was characterized within the brain utilizing the inherent peroxidase activity of Hb in combination with SEM. Rabbit pups with IVH or sham controls were euthanized at 72 h of age followed by saline and freshly prepared 4% PFA perfusion. Afterwards the brains were dissected out from the skulls and immersed in 4% PFA. Brains were prepared and sections at the levels of rostral forebrain (Level 1) were stained for peroxidase activity of Hb or prepared for high magnification SEM as described in the Materials and Methods section. Bright-field microscope analyses were performed on a wide-field Olympus microscope (IX73) and slide scanning were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730. Scanning was performed with a 40 × magnification lens. Images used for illustrations, from regions of interest (ROI), were grabbed with the viewer software NDP.view2 Viewing software. Scale bar of slide scan image indicate 1.0 mm and of ROI images indicate 100 μm. SEM specimens were examined in a Philips/FEI XL 30 FESEM scanning electron microscope using an Everhart-Tornley secondary electron detector. Image processing was done with the Scandium software for simple image acquiring and auto-storage into the Scandium database. Scale bar of SEM images indicate 10 μm.

    Journal: Frontiers in Physiology

    Article Title: High Presence of Extracellular Hemoglobin in the Periventricular White Matter Following Preterm Intraventricular Hemorrhage

    doi: 10.3389/fphys.2016.00330

    Figure Lengend Snippet: Presence of intracellular and extracellular Hb . The presence of RBCs and Hb following IVH was characterized within the brain utilizing the inherent peroxidase activity of Hb in combination with SEM. Rabbit pups with IVH or sham controls were euthanized at 72 h of age followed by saline and freshly prepared 4% PFA perfusion. Afterwards the brains were dissected out from the skulls and immersed in 4% PFA. Brains were prepared and sections at the levels of rostral forebrain (Level 1) were stained for peroxidase activity of Hb or prepared for high magnification SEM as described in the Materials and Methods section. Bright-field microscope analyses were performed on a wide-field Olympus microscope (IX73) and slide scanning were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730. Scanning was performed with a 40 × magnification lens. Images used for illustrations, from regions of interest (ROI), were grabbed with the viewer software NDP.view2 Viewing software. Scale bar of slide scan image indicate 1.0 mm and of ROI images indicate 100 μm. SEM specimens were examined in a Philips/FEI XL 30 FESEM scanning electron microscope using an Everhart-Tornley secondary electron detector. Image processing was done with the Scandium software for simple image acquiring and auto-storage into the Scandium database. Scale bar of SEM images indicate 10 μm.

    Article Snippet: Slide scanning, used for overviews and some of the detailed histological and histochemical descriptions, were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730.

    Techniques: Activity Assay, Staining, Microscopy, Software

    Hb immunolabeling within the brain following preterm rabbit pup IVH . The presence of Hb following IVH was characterized by Hb (displayed as red) immunolabeling of selected regions of interests (ROI) of peroxidase activity corresponding areas, recorded in parallel sections. Rabbit pups with IVH or sham controls were euthanized at 72 h of age followed by saline and freshly prepared 4% PFA perfusion. Afterwards the brains were dissected out from the skulls and immersed in 4% PFA. Brains were prepared and sections at the levels of rostral forebrain (Level 1) and caudal midbrain (Level 4), were immunolabeled for Hb as described in the Materials and Methods Section. Fluorescence microscope analyses were performed on a wide-field Olympus microscope (IX73) and slide scanning were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730. Scanning was performed with a 40 × magnification lens. Images used for illustrations, from ROI's, were grabbed with the viewer software NDP.view2 Viewing software. DAPI labeled cell nuclei are blue. Scale bar of slide scan images indicate 2.5 mm and of ROI images indicate 25 μm.

    Journal: Frontiers in Physiology

    Article Title: High Presence of Extracellular Hemoglobin in the Periventricular White Matter Following Preterm Intraventricular Hemorrhage

    doi: 10.3389/fphys.2016.00330

    Figure Lengend Snippet: Hb immunolabeling within the brain following preterm rabbit pup IVH . The presence of Hb following IVH was characterized by Hb (displayed as red) immunolabeling of selected regions of interests (ROI) of peroxidase activity corresponding areas, recorded in parallel sections. Rabbit pups with IVH or sham controls were euthanized at 72 h of age followed by saline and freshly prepared 4% PFA perfusion. Afterwards the brains were dissected out from the skulls and immersed in 4% PFA. Brains were prepared and sections at the levels of rostral forebrain (Level 1) and caudal midbrain (Level 4), were immunolabeled for Hb as described in the Materials and Methods Section. Fluorescence microscope analyses were performed on a wide-field Olympus microscope (IX73) and slide scanning were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730. Scanning was performed with a 40 × magnification lens. Images used for illustrations, from ROI's, were grabbed with the viewer software NDP.view2 Viewing software. DAPI labeled cell nuclei are blue. Scale bar of slide scan images indicate 2.5 mm and of ROI images indicate 25 μm.

    Article Snippet: Slide scanning, used for overviews and some of the detailed histological and histochemical descriptions, were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730.

    Techniques: Immunolabeling, Activity Assay, Fluorescence, Microscopy, Software, Labeling

    Compatible pollen triggers a decrease in S-RNase transcript levels by a post-transcriptional mechanism. (A) An S 11 -RNAse transgene containing the entire S11-RNase coding sequence and 3'UTR (white) under control of the style-specific chitinase gene promoter and 5'UTR (gray). (B) Northern blot analysis of S 12 S 14 plants expressing the transgenic S 11 -RNase, either without pollination, after a fully incompatible cross (× S 12 S 12 ) or after a fully compatible cross (× S 15 S 16 ) using gene probes against either the S 11 - or the S 12 -RNase transcript. (C) Levels of both S 11 - and S 12 -RNase transcripts (white and black bars, respectively) were quantified by PhosphorImager and reported relative to unpollinated styles. Values are means±s.d. of S-RNase transcript mRNA levels. Asterisks are significantly different (p

    Journal: PLoS ONE

    Article Title: Compatible Pollinations in Solanum chacoense Decrease Both S-RNase and S-RNase mRNA

    doi: 10.1371/journal.pone.0005774

    Figure Lengend Snippet: Compatible pollen triggers a decrease in S-RNase transcript levels by a post-transcriptional mechanism. (A) An S 11 -RNAse transgene containing the entire S11-RNase coding sequence and 3'UTR (white) under control of the style-specific chitinase gene promoter and 5'UTR (gray). (B) Northern blot analysis of S 12 S 14 plants expressing the transgenic S 11 -RNase, either without pollination, after a fully incompatible cross (× S 12 S 12 ) or after a fully compatible cross (× S 15 S 16 ) using gene probes against either the S 11 - or the S 12 -RNase transcript. (C) Levels of both S 11 - and S 12 -RNase transcripts (white and black bars, respectively) were quantified by PhosphorImager and reported relative to unpollinated styles. Values are means±s.d. of S-RNase transcript mRNA levels. Asterisks are significantly different (p

    Article Snippet: All radioactive membranes were imaged and quantitated using a PhosphorImager scanner (Typhoon 9200, Amersham Bioscience).

    Techniques: Sequencing, Northern Blot, Expressing, Transgenic Assay

    S-RNases are not translocated within the style during compatible crosses. The position of pollen tubes from transgenic S 11 S 13 plants expressing the fluorescence marker GFP (white arrowheads) within the styles of S 12 S 12 line 2548 plants was visualized by fluorescence microscopy at various times post pollination. Styles were harvested five hours post-pollination, cultured in vitro and observed by fluorescence microscopy (A). Pollen tubes grown in situ and visualized by aniline blue can also be seen to enter the ovarian region (white arrowheads) 28 hours after pollination and observed by fluorescence microscopy (B). All scale bars are 1 mm. Levels of S 11 -RNase in the upper (U) and lower (L) halves of individual size-selected styles from plants of the S 11 S 12 line 314 left unpollinated, after incompatible (self) and compatible (× S 13 S 14 ) pollinations were measured separately (C). Levels of S 11 -RNase were quantified by PhosphorImager and reported relative to total stylar RNase levels of unpollinated styles (D). Total stylar RNase levels (Σ) were calculated as the sum of upper and lower half measurements. Values are means±s.d. of S 11 -RNase levels from 2 independent experiments. Asterisks are significantly different (p

    Journal: PLoS ONE

    Article Title: Compatible Pollinations in Solanum chacoense Decrease Both S-RNase and S-RNase mRNA

    doi: 10.1371/journal.pone.0005774

    Figure Lengend Snippet: S-RNases are not translocated within the style during compatible crosses. The position of pollen tubes from transgenic S 11 S 13 plants expressing the fluorescence marker GFP (white arrowheads) within the styles of S 12 S 12 line 2548 plants was visualized by fluorescence microscopy at various times post pollination. Styles were harvested five hours post-pollination, cultured in vitro and observed by fluorescence microscopy (A). Pollen tubes grown in situ and visualized by aniline blue can also be seen to enter the ovarian region (white arrowheads) 28 hours after pollination and observed by fluorescence microscopy (B). All scale bars are 1 mm. Levels of S 11 -RNase in the upper (U) and lower (L) halves of individual size-selected styles from plants of the S 11 S 12 line 314 left unpollinated, after incompatible (self) and compatible (× S 13 S 14 ) pollinations were measured separately (C). Levels of S 11 -RNase were quantified by PhosphorImager and reported relative to total stylar RNase levels of unpollinated styles (D). Total stylar RNase levels (Σ) were calculated as the sum of upper and lower half measurements. Values are means±s.d. of S 11 -RNase levels from 2 independent experiments. Asterisks are significantly different (p

    Article Snippet: All radioactive membranes were imaged and quantitated using a PhosphorImager scanner (Typhoon 9200, Amersham Bioscience).

    Techniques: Transgenic Assay, Expressing, Fluorescence, Marker, Microscopy, Cell Culture, In Vitro, In Situ

    Stylar S-RNase levels decrease in compatible but not incompatible crosses. The levels of S 12 -RNase in size-selected and age-matched styles from the homozygous S 12 S 12 line 2548 plants were measured after pollinations with fully incompatible (self) pollen (A), fully compatible pollen from the S 13 S 14 line 582 (B) and semi-compatible pollen from the S 11 S 12 line 314 (C). Each sample contains the entire protein extract from one individual style. Levels of S-RNase were quantified by PhosphorImager and reported relative to unpollinated S 12 S 12 styles (left lanes in each panel) (D). Values are means±s.d. of S 12 -RNase levels from 2 independent experiments. Asterisks are significantly different (p

    Journal: PLoS ONE

    Article Title: Compatible Pollinations in Solanum chacoense Decrease Both S-RNase and S-RNase mRNA

    doi: 10.1371/journal.pone.0005774

    Figure Lengend Snippet: Stylar S-RNase levels decrease in compatible but not incompatible crosses. The levels of S 12 -RNase in size-selected and age-matched styles from the homozygous S 12 S 12 line 2548 plants were measured after pollinations with fully incompatible (self) pollen (A), fully compatible pollen from the S 13 S 14 line 582 (B) and semi-compatible pollen from the S 11 S 12 line 314 (C). Each sample contains the entire protein extract from one individual style. Levels of S-RNase were quantified by PhosphorImager and reported relative to unpollinated S 12 S 12 styles (left lanes in each panel) (D). Values are means±s.d. of S 12 -RNase levels from 2 independent experiments. Asterisks are significantly different (p

    Article Snippet: All radioactive membranes were imaged and quantitated using a PhosphorImager scanner (Typhoon 9200, Amersham Bioscience).

    Techniques:

    Reduced levels of non-self S-RNases are not due to a reduction in stylar S-RNase transcript levels. Levels of mRNA (pollen Lat52; stylar S 12 -RNase, S 11 -RNase and HT-A) were measured in pools of twenty styles taken from the S 11 S 12 line 314 either without pollination, after pollination with incompatible S 12 pollen ( S 11 S 12 × S 12 S 12 ) or after a fully compatible pollination ( S 11 S 12 × S 13 S 14 ) with S 13 , S 14 pollen. Similar amounts of RNA were loaded in each lane (A). Levels of mRNA were quantified by PhosphorImager and reported relative to values in unpollinated styles (for S 11 - and S 12 -RNases) (B). Values are means±s.d. of transcript levels from 2 independent experiments. Asterisks are significantly different (p

    Journal: PLoS ONE

    Article Title: Compatible Pollinations in Solanum chacoense Decrease Both S-RNase and S-RNase mRNA

    doi: 10.1371/journal.pone.0005774

    Figure Lengend Snippet: Reduced levels of non-self S-RNases are not due to a reduction in stylar S-RNase transcript levels. Levels of mRNA (pollen Lat52; stylar S 12 -RNase, S 11 -RNase and HT-A) were measured in pools of twenty styles taken from the S 11 S 12 line 314 either without pollination, after pollination with incompatible S 12 pollen ( S 11 S 12 × S 12 S 12 ) or after a fully compatible pollination ( S 11 S 12 × S 13 S 14 ) with S 13 , S 14 pollen. Similar amounts of RNA were loaded in each lane (A). Levels of mRNA were quantified by PhosphorImager and reported relative to values in unpollinated styles (for S 11 - and S 12 -RNases) (B). Values are means±s.d. of transcript levels from 2 independent experiments. Asterisks are significantly different (p

    Article Snippet: All radioactive membranes were imaged and quantitated using a PhosphorImager scanner (Typhoon 9200, Amersham Bioscience).

    Techniques:

    Pollen mRNA levels remain high and stylar S-RNase transcript levels decrease during compatible crosses. The stigma of S 12 S 12 line 2548 plants, observed twenty-four hours post-pollination with GFP-expressing pollen from transgenic S 12 S 12 plants, shows considerable amounts of GFP when observed by fluorescence microscopy (A), although actively growing pollen tube tips, as visualized by fluorescence microscopy of the fully incompatible cross (line 2548 selfed) stained with aniline blue, have entered the style (B). The stigmatic region (arrowheads) was removed before RNA extraction in order to measure pollen mRNA only in growing pollen tubes. All scale bars are 1 mm. Transcript levels using the indicated probes (pollen Lat52; stylar S 12 -RNase and HT-A) were measured in pools of twenty styles taken from the S 12 S 12 line 2548 either without pollination, after a fully incompatible cross (selfed) or after a fully compatible cross (× S 13 S 14 ). The same amount of RNA as determined by OD measurement was loaded in each lane (C). Levels of each mRNA were quantified by PhosphorImager and reported relative to values in unpollinated styles (for S 12 -RNase) or in compatible crosses (for Lat52 and HT-A). Values are means±s.d. of the mRNA levels from 2 independent experiments. Asterisks are significantly different (p

    Journal: PLoS ONE

    Article Title: Compatible Pollinations in Solanum chacoense Decrease Both S-RNase and S-RNase mRNA

    doi: 10.1371/journal.pone.0005774

    Figure Lengend Snippet: Pollen mRNA levels remain high and stylar S-RNase transcript levels decrease during compatible crosses. The stigma of S 12 S 12 line 2548 plants, observed twenty-four hours post-pollination with GFP-expressing pollen from transgenic S 12 S 12 plants, shows considerable amounts of GFP when observed by fluorescence microscopy (A), although actively growing pollen tube tips, as visualized by fluorescence microscopy of the fully incompatible cross (line 2548 selfed) stained with aniline blue, have entered the style (B). The stigmatic region (arrowheads) was removed before RNA extraction in order to measure pollen mRNA only in growing pollen tubes. All scale bars are 1 mm. Transcript levels using the indicated probes (pollen Lat52; stylar S 12 -RNase and HT-A) were measured in pools of twenty styles taken from the S 12 S 12 line 2548 either without pollination, after a fully incompatible cross (selfed) or after a fully compatible cross (× S 13 S 14 ). The same amount of RNA as determined by OD measurement was loaded in each lane (C). Levels of each mRNA were quantified by PhosphorImager and reported relative to values in unpollinated styles (for S 12 -RNase) or in compatible crosses (for Lat52 and HT-A). Values are means±s.d. of the mRNA levels from 2 independent experiments. Asterisks are significantly different (p

    Article Snippet: All radioactive membranes were imaged and quantitated using a PhosphorImager scanner (Typhoon 9200, Amersham Bioscience).

    Techniques: Expressing, Transgenic Assay, Fluorescence, Microscopy, Staining, RNA Extraction

    Individual unpollinated styles have similar levels of S-RNase when selected by size and age. The levels of S 12 -RNase in the homozygous S 12 S 12 genetic line 2548 (A) and S 11 -RNase in the S 11 S 12 line 314 (B) were measured by Western blot analysis in styles selected to be of the same size and age. Each lane contains the entire protein extract from one individual style. Antibody reaction was monitored using a radio-iodinated secondary antibody, quantified using a PhosphorImager and reported relative to day 0 (C). Styles at day 2 show significantly higher levels of both S 11 - and S 12 -RNase than styles at day 0. Values are means±s.d. of S 11 and S 12 -RNase levels from 2 independent experiments. Asterisks are significantly different (p

    Journal: PLoS ONE

    Article Title: Compatible Pollinations in Solanum chacoense Decrease Both S-RNase and S-RNase mRNA

    doi: 10.1371/journal.pone.0005774

    Figure Lengend Snippet: Individual unpollinated styles have similar levels of S-RNase when selected by size and age. The levels of S 12 -RNase in the homozygous S 12 S 12 genetic line 2548 (A) and S 11 -RNase in the S 11 S 12 line 314 (B) were measured by Western blot analysis in styles selected to be of the same size and age. Each lane contains the entire protein extract from one individual style. Antibody reaction was monitored using a radio-iodinated secondary antibody, quantified using a PhosphorImager and reported relative to day 0 (C). Styles at day 2 show significantly higher levels of both S 11 - and S 12 -RNase than styles at day 0. Values are means±s.d. of S 11 and S 12 -RNase levels from 2 independent experiments. Asterisks are significantly different (p

    Article Snippet: All radioactive membranes were imaged and quantitated using a PhosphorImager scanner (Typhoon 9200, Amersham Bioscience).

    Techniques: Western Blot

    Expression profiling of subsets of SLE reflecting disease activity, using a 135 recombinant antibody microarray. A , Classification of SLE, grouped based solely on disease activity (SLEDAI values); low (3 to 6), mid (9 to 19) and high (22 to 34), based on all 135 antibodies, using a SVM-based leave-one-out cross validation test, expressed in terms of AUC values. AUC values obtained when using only significantly differentially expressed analytes are given within brackets. B , Significantly differentially expressed analytes are shown in a heat map. Twelve differentially expressed analytes, recognized by 14 antibodies, were identified for low versus mid; eight analytes, recognized by 12 antibodies for mid versus high; and 10 analytes recognized by 16 antibodies for low versus high. Green, down-regulated; red, up-regulated; and black, equal levels. The color represent the fold change of a particular marker across all samples within each sample cohort, calculated using the average signal intensities. C , Microarray signal intensities observed for complement proteins, including C1q, C3, C4, and C5, shown as boxplots. The median values are indicated (thick line) and the hinges represent the 25th percentile and the 75th percentile, respectively. D , The correlation between array signal intensities for C1q and SLE disease activity, expressed as SLEDAI-2K.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title:

    doi: 10.1074/mcp.M110.005033

    Figure Lengend Snippet: Expression profiling of subsets of SLE reflecting disease activity, using a 135 recombinant antibody microarray. A , Classification of SLE, grouped based solely on disease activity (SLEDAI values); low (3 to 6), mid (9 to 19) and high (22 to 34), based on all 135 antibodies, using a SVM-based leave-one-out cross validation test, expressed in terms of AUC values. AUC values obtained when using only significantly differentially expressed analytes are given within brackets. B , Significantly differentially expressed analytes are shown in a heat map. Twelve differentially expressed analytes, recognized by 14 antibodies, were identified for low versus mid; eight analytes, recognized by 12 antibodies for mid versus high; and 10 analytes recognized by 16 antibodies for low versus high. Green, down-regulated; red, up-regulated; and black, equal levels. The color represent the fold change of a particular marker across all samples within each sample cohort, calculated using the average signal intensities. C , Microarray signal intensities observed for complement proteins, including C1q, C3, C4, and C5, shown as boxplots. The median values are indicated (thick line) and the hinges represent the 25th percentile and the 75th percentile, respectively. D , The correlation between array signal intensities for C1q and SLE disease activity, expressed as SLEDAI-2K.

    Article Snippet: Finally, the arrays were dried under a stream of nitrogen gas and scanned with a confocal microarray scanner (ScanArray Express, Perkin Elmer Life and Analytical Sciences) at 5-μm resolution, using three different scanner settings.

    Techniques: Expressing, Activity Assay, Recombinant, Microarray, Marker

    Expression profiling of phenotypic subsets of SLE reflecting increased disease severity, using a 135-recombinant-antibody microarray. A , Classification of SLE1, SLE2 and SLE3 based on all 135 antibodies, using a SVM-based leave-one-out cross validation test, expressed in terms of AUC values. AUC values obtained when using only significantly differentially expressed analytes are given within brackets. B , PCA analysis of SLE1 (green), SLE2 (yellow), and SLE3 (red) based on the top 13 significantly differentially antibodies using Qlucore. C , Significantly differentially expressed analytes are shown in a heat map. Twenty-three differentially expressed analytes, recognized by 28 antibodies, were identified for SLE1 versus SLE2; seven analytes, recognized by eight antibodies for SLE2 versus SLE3; and 32 analytes recognized by 47 antibodies for SLE1 versus SLE3. Green, down-regulated; red, up-regulated; and black, equal levels. The color represent the fold change of a particular marker across all samples within each sample cohort, calculated using the average signal intensities. D , Microarray signal intensities observed for key Th1 (IL-2, IL-12, and IFN-γ) and Th2 (IL-4, and IL-10) cytokines shown as boxplots. The median values are indicated (thick line) and the hinges represent the 25th percentile and the 75th percentile, respectively.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title:

    doi: 10.1074/mcp.M110.005033

    Figure Lengend Snippet: Expression profiling of phenotypic subsets of SLE reflecting increased disease severity, using a 135-recombinant-antibody microarray. A , Classification of SLE1, SLE2 and SLE3 based on all 135 antibodies, using a SVM-based leave-one-out cross validation test, expressed in terms of AUC values. AUC values obtained when using only significantly differentially expressed analytes are given within brackets. B , PCA analysis of SLE1 (green), SLE2 (yellow), and SLE3 (red) based on the top 13 significantly differentially antibodies using Qlucore. C , Significantly differentially expressed analytes are shown in a heat map. Twenty-three differentially expressed analytes, recognized by 28 antibodies, were identified for SLE1 versus SLE2; seven analytes, recognized by eight antibodies for SLE2 versus SLE3; and 32 analytes recognized by 47 antibodies for SLE1 versus SLE3. Green, down-regulated; red, up-regulated; and black, equal levels. The color represent the fold change of a particular marker across all samples within each sample cohort, calculated using the average signal intensities. D , Microarray signal intensities observed for key Th1 (IL-2, IL-12, and IFN-γ) and Th2 (IL-4, and IL-10) cytokines shown as boxplots. The median values are indicated (thick line) and the hinges represent the 25th percentile and the 75th percentile, respectively.

    Article Snippet: Finally, the arrays were dried under a stream of nitrogen gas and scanned with a confocal microarray scanner (ScanArray Express, Perkin Elmer Life and Analytical Sciences) at 5-μm resolution, using three different scanner settings.

    Techniques: Expressing, Recombinant, Microarray, Marker

    Expression profiling of serum proteomes from clinical subsets of SSc, dcSSc, and lcSSc, using a 135-recombinant-antibody microarray. A , Classification of dcSSc and lcSSc versus healthy controls based on all 135 antibodies, using a SVM-based leave-one-out cross validation test, expressed in terms of AUC values. AUC values obtained when using only significantly differentially expressed analytes are given within brackets. B , Significantly differentially expressed analytes are shown in a heat map. Seven differentially expressed analytes, recognized by seven antibodies were identified for dcSSc versus controls; five analytes, recognized by five antibodies for lcSSc versus controls; whereas none were observed for dcSSc versus lcSSc. Green, down-regulated; red, up-regulated; and black, equal levels. The color represent the fold change of a particular marker across all samples within each sample cohort, calculated using the average signal intensities.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title:

    doi: 10.1074/mcp.M110.005033

    Figure Lengend Snippet: Expression profiling of serum proteomes from clinical subsets of SSc, dcSSc, and lcSSc, using a 135-recombinant-antibody microarray. A , Classification of dcSSc and lcSSc versus healthy controls based on all 135 antibodies, using a SVM-based leave-one-out cross validation test, expressed in terms of AUC values. AUC values obtained when using only significantly differentially expressed analytes are given within brackets. B , Significantly differentially expressed analytes are shown in a heat map. Seven differentially expressed analytes, recognized by seven antibodies were identified for dcSSc versus controls; five analytes, recognized by five antibodies for lcSSc versus controls; whereas none were observed for dcSSc versus lcSSc. Green, down-regulated; red, up-regulated; and black, equal levels. The color represent the fold change of a particular marker across all samples within each sample cohort, calculated using the average signal intensities.

    Article Snippet: Finally, the arrays were dried under a stream of nitrogen gas and scanned with a confocal microarray scanner (ScanArray Express, Perkin Elmer Life and Analytical Sciences) at 5-μm resolution, using three different scanner settings.

    Techniques: Expressing, Recombinant, Microarray, Marker

    Expression profiling of serum proteomes from SSc and SLE using a 135-recombinant-antibody microarray. The samples were classified using a leave-one-out cross validation approach with a SVM, and illustrated as ROC curves. The patients are ordered by decreasing decision value as assigned by the SVM classifier (middle panel). Differentially expressed analytes are shown in heat maps: green, down-regulated; red, up-regulated; and black, equal levels. A , Classification of healthy controls (blue) versus SSc (red). Four differentially expressed analytes, recognized by five antibodies, were identified. B , Classification of healthy controls (blue) versus SLE (red). Forty nonredundant differentially expressed analytes, recognized by 58 antibodies were identified, of which the 25 highest ranked, i.e. significantly differentially expressed ( p values), analytes are shown. C , Classification of SSc (blue) versus SLE (red). Forty-two nonredundant differentially expressed analytes, recognized by 62 antibodies were identified, of which the 25 highest ranked analytes ( p values) are shown.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title:

    doi: 10.1074/mcp.M110.005033

    Figure Lengend Snippet: Expression profiling of serum proteomes from SSc and SLE using a 135-recombinant-antibody microarray. The samples were classified using a leave-one-out cross validation approach with a SVM, and illustrated as ROC curves. The patients are ordered by decreasing decision value as assigned by the SVM classifier (middle panel). Differentially expressed analytes are shown in heat maps: green, down-regulated; red, up-regulated; and black, equal levels. A , Classification of healthy controls (blue) versus SSc (red). Four differentially expressed analytes, recognized by five antibodies, were identified. B , Classification of healthy controls (blue) versus SLE (red). Forty nonredundant differentially expressed analytes, recognized by 58 antibodies were identified, of which the 25 highest ranked, i.e. significantly differentially expressed ( p values), analytes are shown. C , Classification of SSc (blue) versus SLE (red). Forty-two nonredundant differentially expressed analytes, recognized by 62 antibodies were identified, of which the 25 highest ranked analytes ( p values) are shown.

    Article Snippet: Finally, the arrays were dried under a stream of nitrogen gas and scanned with a confocal microarray scanner (ScanArray Express, Perkin Elmer Life and Analytical Sciences) at 5-μm resolution, using three different scanner settings.

    Techniques: Expressing, Recombinant, Microarray

    Expression profiling of phenotypic subsets of SLE reflecting increased disease severity, including SLE1 (least symptoms), SLE2, and SLE3 (most severe symptoms), using a 135-recombinant-antibody microarray. The samples were classified using a leave-one-out cross validation approach with a SVM, and illustrated as ROC curves. The patients are ordered by decreasing decision value as assigned by the SVM classifier ( middle panel ). Differentially expressed analytes are shown in heat maps: green, down-regulated; red, up-regulated; and black, equal levels. The color represent the fold change of a particular marker across all samples within each sample cohort, calculated using the average signal intensities. A , Classification of SLE1 (red) versus controls (blue). B , Classification of SLE2 (red) versus controls (blue). C , Classification of SLE3 (red) versus controls (blue). The top 25 highest ranked, i.e. significantly differentially expressed ( p values), analytes are shown. D , Fifteen significantly differentially expressed analytes, recognized by 17 antibodies, were identified for SLE1 versus controls; 29 analytes, recognized by 36 antibodies for SLE2 versus controls; and 44 analytes recognized by 72 antibodies for SLE3 versus controls.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title:

    doi: 10.1074/mcp.M110.005033

    Figure Lengend Snippet: Expression profiling of phenotypic subsets of SLE reflecting increased disease severity, including SLE1 (least symptoms), SLE2, and SLE3 (most severe symptoms), using a 135-recombinant-antibody microarray. The samples were classified using a leave-one-out cross validation approach with a SVM, and illustrated as ROC curves. The patients are ordered by decreasing decision value as assigned by the SVM classifier ( middle panel ). Differentially expressed analytes are shown in heat maps: green, down-regulated; red, up-regulated; and black, equal levels. The color represent the fold change of a particular marker across all samples within each sample cohort, calculated using the average signal intensities. A , Classification of SLE1 (red) versus controls (blue). B , Classification of SLE2 (red) versus controls (blue). C , Classification of SLE3 (red) versus controls (blue). The top 25 highest ranked, i.e. significantly differentially expressed ( p values), analytes are shown. D , Fifteen significantly differentially expressed analytes, recognized by 17 antibodies, were identified for SLE1 versus controls; 29 analytes, recognized by 36 antibodies for SLE2 versus controls; and 44 analytes recognized by 72 antibodies for SLE3 versus controls.

    Article Snippet: Finally, the arrays were dried under a stream of nitrogen gas and scanned with a confocal microarray scanner (ScanArray Express, Perkin Elmer Life and Analytical Sciences) at 5-μm resolution, using three different scanner settings.

    Techniques: Expressing, Recombinant, Microarray, Marker

    Expression profiling of phenotypic subsets of SLE reflecting increased disease severity and SSc, using a 135-recombinant-antibody microarray. The samples were classified using a leave-one-out cross validation approach with a SVM, and illustrated as ROC curves. The patients are ordered by decreasing decision value as assigned by the SVM classifier ( middle panel ). Differentially expressed analytes are shown in heat maps: green, down-regulated; red, up-regulated; and black, equal levels. The color represent the fold change of a particular marker across all samples within each sample cohort, calculated using the average signal intensities. A , Classification of SLE1 (red) versus SSc (blue). B , Classification of SLE2 (red) versus SSc (blue). C , Classification of SLE3 (red) versus SSc (blue). The top 25 highest ranked, i.e. significantly differentially expressed ( p values), analytes are shown. D , Three significantly differentially expressed analytes, recognized by three antibodies, were identified for SLE1 versus SSc; 36 analytes, recognized by 47 antibodies for SLE2 versus SSc; and 49 analytes recognized by 79 antibodies for SLE3 versus SSc.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title:

    doi: 10.1074/mcp.M110.005033

    Figure Lengend Snippet: Expression profiling of phenotypic subsets of SLE reflecting increased disease severity and SSc, using a 135-recombinant-antibody microarray. The samples were classified using a leave-one-out cross validation approach with a SVM, and illustrated as ROC curves. The patients are ordered by decreasing decision value as assigned by the SVM classifier ( middle panel ). Differentially expressed analytes are shown in heat maps: green, down-regulated; red, up-regulated; and black, equal levels. The color represent the fold change of a particular marker across all samples within each sample cohort, calculated using the average signal intensities. A , Classification of SLE1 (red) versus SSc (blue). B , Classification of SLE2 (red) versus SSc (blue). C , Classification of SLE3 (red) versus SSc (blue). The top 25 highest ranked, i.e. significantly differentially expressed ( p values), analytes are shown. D , Three significantly differentially expressed analytes, recognized by three antibodies, were identified for SLE1 versus SSc; 36 analytes, recognized by 47 antibodies for SLE2 versus SSc; and 49 analytes recognized by 79 antibodies for SLE3 versus SSc.

    Article Snippet: Finally, the arrays were dried under a stream of nitrogen gas and scanned with a confocal microarray scanner (ScanArray Express, Perkin Elmer Life and Analytical Sciences) at 5-μm resolution, using three different scanner settings.

    Techniques: Expressing, Recombinant, Microarray, Marker

    Expression profiling of subsets of SLE3 reflecting disease activity, using a 135 recombinant antibody microarray. Classification of SLE3 subsets, grouped based on disease activity (SLEDAI values); SLE3 low (blue) (mean 13, range 10–16), SLE high (red) (mean 24, range 17–32), based on all 135 antibodies, using a SVM-based leave-one-out cross validation test, expressed in terms of AUC values. The patients are ordered by decreasing decision value as assigned by the SVM classifier ( middle panel ). The 20 signficantly differentially expressed analytes, recognized by 31 antibodies, are shown in a heat map. SSc. Green, down-regulated; red, up-regulated; and black, equal levels.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title:

    doi: 10.1074/mcp.M110.005033

    Figure Lengend Snippet: Expression profiling of subsets of SLE3 reflecting disease activity, using a 135 recombinant antibody microarray. Classification of SLE3 subsets, grouped based on disease activity (SLEDAI values); SLE3 low (blue) (mean 13, range 10–16), SLE high (red) (mean 24, range 17–32), based on all 135 antibodies, using a SVM-based leave-one-out cross validation test, expressed in terms of AUC values. The patients are ordered by decreasing decision value as assigned by the SVM classifier ( middle panel ). The 20 signficantly differentially expressed analytes, recognized by 31 antibodies, are shown in a heat map. SSc. Green, down-regulated; red, up-regulated; and black, equal levels.

    Article Snippet: Finally, the arrays were dried under a stream of nitrogen gas and scanned with a confocal microarray scanner (ScanArray Express, Perkin Elmer Life and Analytical Sciences) at 5-μm resolution, using three different scanner settings.

    Techniques: Expressing, Activity Assay, Recombinant, Microarray

    Case #4. Baseline magnetic resonance imaging (MRI) (A) , fluorothymidine (FLT)–positron-emission tomography (PET) (B) , and fused PET/MRI (C) . The 1.6-cm cerebellar lesion (arrow) with FLT avidity showed SUV ratio of 18.2.

    Journal: Frontiers in Oncology

    Article Title: Targeted Therapy and Immunotherapy Response Assessment with F-18 Fluorothymidine Positron-Emission Tomography/Magnetic Resonance Imaging in Melanoma Brain Metastasis: A Pilot Study

    doi: 10.3389/fonc.2018.00018

    Figure Lengend Snippet: Case #4. Baseline magnetic resonance imaging (MRI) (A) , fluorothymidine (FLT)–positron-emission tomography (PET) (B) , and fused PET/MRI (C) . The 1.6-cm cerebellar lesion (arrow) with FLT avidity showed SUV ratio of 18.2.

    Article Snippet: Enrolled subjects would undergo baseline and follow-up FLT–PET/MRI scans performed at least 2 weeks after therapy initiation on a hybrid PET/MRI scanner (Biograph mMR, Siemens).

    Techniques: Magnetic Resonance Imaging, Positron Emission Tomography

    Case #2. Baseline magnetic resonance imaging (MRI) (A) , fluorothymidine (FLT)–positron-emission tomography (PET) (B) , and fused PET/MRI (C) ; follow-up MRI (D) , FLT–PET (E) , and fused PET/MRI (F) at 1 month. Among multiple melanoma brain metastases, the left anterior frontal lobe lesion (arrow) showed a slight interval increase in FLT uptake and size [SUV ratio (SUVR) from 12.6 to 13.5; size from 1.7 to 2.0 cm]; the left lateral frontal lobe lesion (arrowhead) demonstrated interval decrease in FLT uptake from SUVR of 11.3–6.3, while the size remained stable at 2.3 cm. Same T1-weighted MRI parameters as in Figure 1 .

    Journal: Frontiers in Oncology

    Article Title: Targeted Therapy and Immunotherapy Response Assessment with F-18 Fluorothymidine Positron-Emission Tomography/Magnetic Resonance Imaging in Melanoma Brain Metastasis: A Pilot Study

    doi: 10.3389/fonc.2018.00018

    Figure Lengend Snippet: Case #2. Baseline magnetic resonance imaging (MRI) (A) , fluorothymidine (FLT)–positron-emission tomography (PET) (B) , and fused PET/MRI (C) ; follow-up MRI (D) , FLT–PET (E) , and fused PET/MRI (F) at 1 month. Among multiple melanoma brain metastases, the left anterior frontal lobe lesion (arrow) showed a slight interval increase in FLT uptake and size [SUV ratio (SUVR) from 12.6 to 13.5; size from 1.7 to 2.0 cm]; the left lateral frontal lobe lesion (arrowhead) demonstrated interval decrease in FLT uptake from SUVR of 11.3–6.3, while the size remained stable at 2.3 cm. Same T1-weighted MRI parameters as in Figure 1 .

    Article Snippet: Enrolled subjects would undergo baseline and follow-up FLT–PET/MRI scans performed at least 2 weeks after therapy initiation on a hybrid PET/MRI scanner (Biograph mMR, Siemens).

    Techniques: Magnetic Resonance Imaging, Positron Emission Tomography

    Case #2. Baseline magnetic resonance imaging (MRI) (A) , fluorothymidine (FLT)–positron-emission tomography (PET) (B) , and fused PET/MRI (C) ; follow-up MRI (D) , FLT-PET (E) , and fused PET/MRI (F) . The left cerebellar lesion (arrow) showed interval increase in FLT uptake and size [SUV ratio from 11.2 to 18.9; size 1.0–1.5 cm].

    Journal: Frontiers in Oncology

    Article Title: Targeted Therapy and Immunotherapy Response Assessment with F-18 Fluorothymidine Positron-Emission Tomography/Magnetic Resonance Imaging in Melanoma Brain Metastasis: A Pilot Study

    doi: 10.3389/fonc.2018.00018

    Figure Lengend Snippet: Case #2. Baseline magnetic resonance imaging (MRI) (A) , fluorothymidine (FLT)–positron-emission tomography (PET) (B) , and fused PET/MRI (C) ; follow-up MRI (D) , FLT-PET (E) , and fused PET/MRI (F) . The left cerebellar lesion (arrow) showed interval increase in FLT uptake and size [SUV ratio from 11.2 to 18.9; size 1.0–1.5 cm].

    Article Snippet: Enrolled subjects would undergo baseline and follow-up FLT–PET/MRI scans performed at least 2 weeks after therapy initiation on a hybrid PET/MRI scanner (Biograph mMR, Siemens).

    Techniques: Magnetic Resonance Imaging, Positron Emission Tomography

    Case #3. Baseline magnetic resonance imaging (MRI) (A) , fluorothymidine (FLT)–positron-emission tomography (PET) (B) , and fused PET/MRI (C) . The 1.1 cm right posterior, medial temporal lobe lesion (arrow) showed FLT avidity with SUV ratio of 7.5.

    Journal: Frontiers in Oncology

    Article Title: Targeted Therapy and Immunotherapy Response Assessment with F-18 Fluorothymidine Positron-Emission Tomography/Magnetic Resonance Imaging in Melanoma Brain Metastasis: A Pilot Study

    doi: 10.3389/fonc.2018.00018

    Figure Lengend Snippet: Case #3. Baseline magnetic resonance imaging (MRI) (A) , fluorothymidine (FLT)–positron-emission tomography (PET) (B) , and fused PET/MRI (C) . The 1.1 cm right posterior, medial temporal lobe lesion (arrow) showed FLT avidity with SUV ratio of 7.5.

    Article Snippet: Enrolled subjects would undergo baseline and follow-up FLT–PET/MRI scans performed at least 2 weeks after therapy initiation on a hybrid PET/MRI scanner (Biograph mMR, Siemens).

    Techniques: Magnetic Resonance Imaging, Positron Emission Tomography

    Case #5. Baseline magnetic resonance imaging (MRI) (A) , fluorothymidine (FLT)-positron-emission tomography (PET) (B) , and fused PET/MRI (C) . Multiple FLT avid brain metastases with Gadolinium contrast enhancement were present. The largest lesion in the left occipital lobe with central necrosis (arrow) measured 2.9 cm with SUV ratio of 14.9.

    Journal: Frontiers in Oncology

    Article Title: Targeted Therapy and Immunotherapy Response Assessment with F-18 Fluorothymidine Positron-Emission Tomography/Magnetic Resonance Imaging in Melanoma Brain Metastasis: A Pilot Study

    doi: 10.3389/fonc.2018.00018

    Figure Lengend Snippet: Case #5. Baseline magnetic resonance imaging (MRI) (A) , fluorothymidine (FLT)-positron-emission tomography (PET) (B) , and fused PET/MRI (C) . Multiple FLT avid brain metastases with Gadolinium contrast enhancement were present. The largest lesion in the left occipital lobe with central necrosis (arrow) measured 2.9 cm with SUV ratio of 14.9.

    Article Snippet: Enrolled subjects would undergo baseline and follow-up FLT–PET/MRI scans performed at least 2 weeks after therapy initiation on a hybrid PET/MRI scanner (Biograph mMR, Siemens).

    Techniques: Magnetic Resonance Imaging, Positron Emission Tomography

    Case #1. Baseline magnetic resonance imaging (MRI) (A) , fluorothymidine (FLT)–positron-emission tomography (PET) (B) , and fused PET/MRI (C) ; follow-up MRI (D) , FLT–PET (E) , and fused PET/MRI (F) at 3 weeks. Bilateral supratentorial brain lesions of greater than 1 cm demonstrate variable mild to intense FLT avidity. They showed a greater proliferative reduction compared with the size reduction at 1-month follow-up. Gadolinium-enhanced T1-weighted spin echo with TR 708 ms, TE 8.4 ms, and 90° flip angle, 5 mm slice thickness.

    Journal: Frontiers in Oncology

    Article Title: Targeted Therapy and Immunotherapy Response Assessment with F-18 Fluorothymidine Positron-Emission Tomography/Magnetic Resonance Imaging in Melanoma Brain Metastasis: A Pilot Study

    doi: 10.3389/fonc.2018.00018

    Figure Lengend Snippet: Case #1. Baseline magnetic resonance imaging (MRI) (A) , fluorothymidine (FLT)–positron-emission tomography (PET) (B) , and fused PET/MRI (C) ; follow-up MRI (D) , FLT–PET (E) , and fused PET/MRI (F) at 3 weeks. Bilateral supratentorial brain lesions of greater than 1 cm demonstrate variable mild to intense FLT avidity. They showed a greater proliferative reduction compared with the size reduction at 1-month follow-up. Gadolinium-enhanced T1-weighted spin echo with TR 708 ms, TE 8.4 ms, and 90° flip angle, 5 mm slice thickness.

    Article Snippet: Enrolled subjects would undergo baseline and follow-up FLT–PET/MRI scans performed at least 2 weeks after therapy initiation on a hybrid PET/MRI scanner (Biograph mMR, Siemens).

    Techniques: Magnetic Resonance Imaging, Positron Emission Tomography, Mass Spectrometry

    Axial FLAIR (A) Reveals prominent ventricles and sulcal spaces (white arrow)-cerebral atrophy; Axial T1wMPRAGE (D) Reveals hippocampal atrophy; Axial fused PET MRI (B and E) Sagittal (C) and coronal PET (F) Reveal bilateral fronto-temporal and parietal hypometabolism (white arrowhead). Note well preserved glucose metabolism in sensorimotor cortex and occipital lobes (white arrow) on sagittal PET images consistent with established AD. Statistical parametric map surface display (G) Shows marked hypometabolism (blue) in corresponding areas of bilateral cerebral hemispheres

    Journal: The Indian Journal of Radiology & Imaging

    Article Title: Integrated 18F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging (18F-FDG PET/MRI), a multimodality approach for comprehensive evaluation of dementia patients: A pictorial essay

    doi: 10.4103/0971-3026.169449

    Figure Lengend Snippet: Axial FLAIR (A) Reveals prominent ventricles and sulcal spaces (white arrow)-cerebral atrophy; Axial T1wMPRAGE (D) Reveals hippocampal atrophy; Axial fused PET MRI (B and E) Sagittal (C) and coronal PET (F) Reveal bilateral fronto-temporal and parietal hypometabolism (white arrowhead). Note well preserved glucose metabolism in sensorimotor cortex and occipital lobes (white arrow) on sagittal PET images consistent with established AD. Statistical parametric map surface display (G) Shows marked hypometabolism (blue) in corresponding areas of bilateral cerebral hemispheres

    Article Snippet: Integrated PET/MRI was performed on 48 patients with suspected dementia from Jan 2013 to Dec 2014 using simultaneous hybrid PET/MRI scanner (Siemens Biograph mMR; Siemens Healthcare, Erlangen, Germany).

    Techniques: Positron Emission Tomography, Magnetic Resonance Imaging

    Axial FLAIR Images (A and D) Reveal extensive non-enhancing white matter hyperintensities in bilateral periventricular and deep white matter (white arrows) with cerebral and cerebellar atrophy (white arrowhead) (G) Axial PET (B and H), fused PET/MRI (C and I) and coronal fused PET MRI reveal reduced FDG uptake inbilateral frontal, temporal (R > L) and cerebellum (white arrowheads) with relatively spared bilateral parieto-occipital lobes. Axial FLAIR image showing symmetrical bilateral temporal lobes and hippocampi with apparent normal volume and signal intensity (broken white line)

    Journal: The Indian Journal of Radiology & Imaging

    Article Title: Integrated 18F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging (18F-FDG PET/MRI), a multimodality approach for comprehensive evaluation of dementia patients: A pictorial essay

    doi: 10.4103/0971-3026.169449

    Figure Lengend Snippet: Axial FLAIR Images (A and D) Reveal extensive non-enhancing white matter hyperintensities in bilateral periventricular and deep white matter (white arrows) with cerebral and cerebellar atrophy (white arrowhead) (G) Axial PET (B and H), fused PET/MRI (C and I) and coronal fused PET MRI reveal reduced FDG uptake inbilateral frontal, temporal (R > L) and cerebellum (white arrowheads) with relatively spared bilateral parieto-occipital lobes. Axial FLAIR image showing symmetrical bilateral temporal lobes and hippocampi with apparent normal volume and signal intensity (broken white line)

    Article Snippet: Integrated PET/MRI was performed on 48 patients with suspected dementia from Jan 2013 to Dec 2014 using simultaneous hybrid PET/MRI scanner (Siemens Biograph mMR; Siemens Healthcare, Erlangen, Germany).

    Techniques: Positron Emission Tomography, Magnetic Resonance Imaging

    Axial T1w MPRAGE, FDG PET and fused PET/MRI images at the level of hippocampus (row B) reveals bilateral (more on left side) hippocampal atrophy with predominantly left sided anterior temporal cortex atrophy (white arrows) and corresponding anterior polar temporal hypometabolism (white thick arrows). Similar images at a higher level (row A) depict absence of any fronto-parietal atrophy or hypometabolism (white arrowheads) which in conjunction with patient's semantic memory loss confirmed diagnosis of semantic dementia

    Journal: The Indian Journal of Radiology & Imaging

    Article Title: Integrated 18F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging (18F-FDG PET/MRI), a multimodality approach for comprehensive evaluation of dementia patients: A pictorial essay

    doi: 10.4103/0971-3026.169449

    Figure Lengend Snippet: Axial T1w MPRAGE, FDG PET and fused PET/MRI images at the level of hippocampus (row B) reveals bilateral (more on left side) hippocampal atrophy with predominantly left sided anterior temporal cortex atrophy (white arrows) and corresponding anterior polar temporal hypometabolism (white thick arrows). Similar images at a higher level (row A) depict absence of any fronto-parietal atrophy or hypometabolism (white arrowheads) which in conjunction with patient's semantic memory loss confirmed diagnosis of semantic dementia

    Article Snippet: Integrated PET/MRI was performed on 48 patients with suspected dementia from Jan 2013 to Dec 2014 using simultaneous hybrid PET/MRI scanner (Siemens Biograph mMR; Siemens Healthcare, Erlangen, Germany).

    Techniques: Positron Emission Tomography, Magnetic Resonance Imaging

    Axial FLAIR (A) reveals multiple non-enhancing white matter hyperintensities in bilateral periventricular region (white arrow) with bilateral fronto-parietal hypometabolism (white thick arrows) on axial PET (B) and fused PET/MRI (C) Axial SWI revealed multiple subcortical and deep old hemorrhagic residua in bilateral cerebral hemispheres (D) and cerebellum (F) In addition, left occipital lobe chronic infarct and gliosis was noted (white arrowhead) (D) and corresponding hypometabolism on axial fused PET/MRI image (white arrowhead) (E) These findings favored vascular dementia than AD

    Journal: The Indian Journal of Radiology & Imaging

    Article Title: Integrated 18F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging (18F-FDG PET/MRI), a multimodality approach for comprehensive evaluation of dementia patients: A pictorial essay

    doi: 10.4103/0971-3026.169449

    Figure Lengend Snippet: Axial FLAIR (A) reveals multiple non-enhancing white matter hyperintensities in bilateral periventricular region (white arrow) with bilateral fronto-parietal hypometabolism (white thick arrows) on axial PET (B) and fused PET/MRI (C) Axial SWI revealed multiple subcortical and deep old hemorrhagic residua in bilateral cerebral hemispheres (D) and cerebellum (F) In addition, left occipital lobe chronic infarct and gliosis was noted (white arrowhead) (D) and corresponding hypometabolism on axial fused PET/MRI image (white arrowhead) (E) These findings favored vascular dementia than AD

    Article Snippet: Integrated PET/MRI was performed on 48 patients with suspected dementia from Jan 2013 to Dec 2014 using simultaneous hybrid PET/MRI scanner (Siemens Biograph mMR; Siemens Healthcare, Erlangen, Germany).

    Techniques: Positron Emission Tomography, Magnetic Resonance Imaging

    (A) Asymmetrical focal areas of hypometabolism in right temporal region (white arrow) on axial PET and T2w MRI images (B) Axial fused PET-MRI (C) Coronal T1w MPRAGE (D) Suggesting old infarcts consistent with VaD. Surface display of statistical parametric map (E) and coronal fused PET/MRI (F) with corresponding areas of encephalomalacia and gliosis (white arrow) on axial FLAIR (G) Showing marked hypometabolism (blue) in right temporal and left temporo occipital regions, compared to standard database of Scenium software

    Journal: The Indian Journal of Radiology & Imaging

    Article Title: Integrated 18F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging (18F-FDG PET/MRI), a multimodality approach for comprehensive evaluation of dementia patients: A pictorial essay

    doi: 10.4103/0971-3026.169449

    Figure Lengend Snippet: (A) Asymmetrical focal areas of hypometabolism in right temporal region (white arrow) on axial PET and T2w MRI images (B) Axial fused PET-MRI (C) Coronal T1w MPRAGE (D) Suggesting old infarcts consistent with VaD. Surface display of statistical parametric map (E) and coronal fused PET/MRI (F) with corresponding areas of encephalomalacia and gliosis (white arrow) on axial FLAIR (G) Showing marked hypometabolism (blue) in right temporal and left temporo occipital regions, compared to standard database of Scenium software

    Article Snippet: Integrated PET/MRI was performed on 48 patients with suspected dementia from Jan 2013 to Dec 2014 using simultaneous hybrid PET/MRI scanner (Siemens Biograph mMR; Siemens Healthcare, Erlangen, Germany).

    Techniques: Positron Emission Tomography, Magnetic Resonance Imaging, Software

    Severe bilateral hippocampal atrophy evident by severely reduced hippocampal volume (white arrows) (Left more than right) on Axial MRI images (A) With prominent bilateral frontal and temporal hypometabolism on axial fused PET MRI images (white arrowhead) (B and C). Note preserved parietal metabolism on axial and sagittal fused PET MRI images (white thick arrows) (D)

    Journal: The Indian Journal of Radiology & Imaging

    Article Title: Integrated 18F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging (18F-FDG PET/MRI), a multimodality approach for comprehensive evaluation of dementia patients: A pictorial essay

    doi: 10.4103/0971-3026.169449

    Figure Lengend Snippet: Severe bilateral hippocampal atrophy evident by severely reduced hippocampal volume (white arrows) (Left more than right) on Axial MRI images (A) With prominent bilateral frontal and temporal hypometabolism on axial fused PET MRI images (white arrowhead) (B and C). Note preserved parietal metabolism on axial and sagittal fused PET MRI images (white thick arrows) (D)

    Article Snippet: Integrated PET/MRI was performed on 48 patients with suspected dementia from Jan 2013 to Dec 2014 using simultaneous hybrid PET/MRI scanner (Siemens Biograph mMR; Siemens Healthcare, Erlangen, Germany).

    Techniques: Magnetic Resonance Imaging, Positron Emission Tomography

    Axial T1wMPRAGE, PET and fused PET/MRI (right to left) (A) Reveal bilateral cortical hypometabolism (white thick arrows) with loss of normal gray white matter differentiation on PET. Axial SWI (B) Depicts discrete subacute hemorrhagic infarct (white arrowhead) increased uptake and subtle gyriform post contrast enhancement (white arrowhead) in right parieto-occipital region Restricted diffusion on ADC with cortical “ribboning” on DWI images (white arrows) at multiple axial levels (C and D) Quantitative PET analysis (E) Depicts more prominent bilateral frontal hypometabolism

    Journal: The Indian Journal of Radiology & Imaging

    Article Title: Integrated 18F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging (18F-FDG PET/MRI), a multimodality approach for comprehensive evaluation of dementia patients: A pictorial essay

    doi: 10.4103/0971-3026.169449

    Figure Lengend Snippet: Axial T1wMPRAGE, PET and fused PET/MRI (right to left) (A) Reveal bilateral cortical hypometabolism (white thick arrows) with loss of normal gray white matter differentiation on PET. Axial SWI (B) Depicts discrete subacute hemorrhagic infarct (white arrowhead) increased uptake and subtle gyriform post contrast enhancement (white arrowhead) in right parieto-occipital region Restricted diffusion on ADC with cortical “ribboning” on DWI images (white arrows) at multiple axial levels (C and D) Quantitative PET analysis (E) Depicts more prominent bilateral frontal hypometabolism

    Article Snippet: Integrated PET/MRI was performed on 48 patients with suspected dementia from Jan 2013 to Dec 2014 using simultaneous hybrid PET/MRI scanner (Siemens Biograph mMR; Siemens Healthcare, Erlangen, Germany).

    Techniques: Positron Emission Tomography, Magnetic Resonance Imaging, Diffusion-based Assay

    Axial T1wMPRAGE (A) Reveals cerebral atrophy and significant bilateral fronto-parietal and temporal hypometabolism (white arrowheads) on axial PET (B) Axial fused PET/MRI (C) and coronal PET (F) Consistent with established AD; Note asymmetrical glucose metabolism of right fronto-parieto-temporal region; Axial T1wMPRAGE (D) and FLAIR images (E) Reveal loss of hippocampal volume and sclerosis (white arrows) affecting the right side more than the left. Statistical parametric map surface display (G) Shows marked asymmetrical bilateral cerebral hemisphere hypometabolism (blue) (R > L)

    Journal: The Indian Journal of Radiology & Imaging

    Article Title: Integrated 18F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging (18F-FDG PET/MRI), a multimodality approach for comprehensive evaluation of dementia patients: A pictorial essay

    doi: 10.4103/0971-3026.169449

    Figure Lengend Snippet: Axial T1wMPRAGE (A) Reveals cerebral atrophy and significant bilateral fronto-parietal and temporal hypometabolism (white arrowheads) on axial PET (B) Axial fused PET/MRI (C) and coronal PET (F) Consistent with established AD; Note asymmetrical glucose metabolism of right fronto-parieto-temporal region; Axial T1wMPRAGE (D) and FLAIR images (E) Reveal loss of hippocampal volume and sclerosis (white arrows) affecting the right side more than the left. Statistical parametric map surface display (G) Shows marked asymmetrical bilateral cerebral hemisphere hypometabolism (blue) (R > L)

    Article Snippet: Integrated PET/MRI was performed on 48 patients with suspected dementia from Jan 2013 to Dec 2014 using simultaneous hybrid PET/MRI scanner (Siemens Biograph mMR; Siemens Healthcare, Erlangen, Germany).

    Techniques: Positron Emission Tomography, Magnetic Resonance Imaging

    Axial T1wMPRAGE, PET and fused PET/MRI (right to left) (A and B) Reveal significant left fronto-parieto-occipital and temporal hypometabolism (white arrowheads). “Ribbon-like” cortico-subcortical altered intensity in left fronto-parieto-occipital and temporal cortices (white arrows) and right parieto-occipital regions in axial DWI images (C and D) with corresponding diffusion restriction (E) Corresponding “ribboning” (C) and hypometabolism (white arrowheads) (C1). Quantitative analysis (F) shows left fronto-parietal-temporal hypometabolism (blue) out of proportion to the contralateral cortex

    Journal: The Indian Journal of Radiology & Imaging

    Article Title: Integrated 18F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging (18F-FDG PET/MRI), a multimodality approach for comprehensive evaluation of dementia patients: A pictorial essay

    doi: 10.4103/0971-3026.169449

    Figure Lengend Snippet: Axial T1wMPRAGE, PET and fused PET/MRI (right to left) (A and B) Reveal significant left fronto-parieto-occipital and temporal hypometabolism (white arrowheads). “Ribbon-like” cortico-subcortical altered intensity in left fronto-parieto-occipital and temporal cortices (white arrows) and right parieto-occipital regions in axial DWI images (C and D) with corresponding diffusion restriction (E) Corresponding “ribboning” (C) and hypometabolism (white arrowheads) (C1). Quantitative analysis (F) shows left fronto-parietal-temporal hypometabolism (blue) out of proportion to the contralateral cortex

    Article Snippet: Integrated PET/MRI was performed on 48 patients with suspected dementia from Jan 2013 to Dec 2014 using simultaneous hybrid PET/MRI scanner (Siemens Biograph mMR; Siemens Healthcare, Erlangen, Germany).

    Techniques: Positron Emission Tomography, Magnetic Resonance Imaging, Diffusion-based Assay

    Sagittal PET (B) Fused PET/MRI (C) Axial PET (E) and axial fused PET/MRI (I and F) reveal bilateral fronto-temporal hypometabolism (white arrowheads) with apparently normal parietal FDG uptake and hippocampal volume on axial T1wMPRAGE images (white arrow) (D) Also, Sagittal and axial T1wMPRAGE MRI images (A and H) reveal features of gliosis and encephalomalacia in bilateral parasagittal frontoparietal cortex (white arrowhead) and a midline old fax hematoma (white arrowhead) and hemorrhagic residua noted on axial SWI images (white arrowhead) (G) These features favored the diagnosis of FTD

    Journal: The Indian Journal of Radiology & Imaging

    Article Title: Integrated 18F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging (18F-FDG PET/MRI), a multimodality approach for comprehensive evaluation of dementia patients: A pictorial essay

    doi: 10.4103/0971-3026.169449

    Figure Lengend Snippet: Sagittal PET (B) Fused PET/MRI (C) Axial PET (E) and axial fused PET/MRI (I and F) reveal bilateral fronto-temporal hypometabolism (white arrowheads) with apparently normal parietal FDG uptake and hippocampal volume on axial T1wMPRAGE images (white arrow) (D) Also, Sagittal and axial T1wMPRAGE MRI images (A and H) reveal features of gliosis and encephalomalacia in bilateral parasagittal frontoparietal cortex (white arrowhead) and a midline old fax hematoma (white arrowhead) and hemorrhagic residua noted on axial SWI images (white arrowhead) (G) These features favored the diagnosis of FTD

    Article Snippet: Integrated PET/MRI was performed on 48 patients with suspected dementia from Jan 2013 to Dec 2014 using simultaneous hybrid PET/MRI scanner (Siemens Biograph mMR; Siemens Healthcare, Erlangen, Germany).

    Techniques: Positron Emission Tomography, Magnetic Resonance Imaging

    Axial SWI MRI Images (A and D) Reveal punctate old hemorrhagic residua in bilateral frontal and parietal regions (white arrows) with corresponding bilaterally symmetrical fronto-temporal hypometabolism (white arrowheads) on axial PET (B) and fused PET/MRI images (C) Also, on axial FLAIR MRI images (E and F) Ill defined non enhancing white matter changes in bilateral periventricular regions, corona radiata and centrum semiovale (white arrowheads) were also noted

    Journal: The Indian Journal of Radiology & Imaging

    Article Title: Integrated 18F-fluorodeoxyglucose positron emission tomography magnetic resonance imaging (18F-FDG PET/MRI), a multimodality approach for comprehensive evaluation of dementia patients: A pictorial essay

    doi: 10.4103/0971-3026.169449

    Figure Lengend Snippet: Axial SWI MRI Images (A and D) Reveal punctate old hemorrhagic residua in bilateral frontal and parietal regions (white arrows) with corresponding bilaterally symmetrical fronto-temporal hypometabolism (white arrowheads) on axial PET (B) and fused PET/MRI images (C) Also, on axial FLAIR MRI images (E and F) Ill defined non enhancing white matter changes in bilateral periventricular regions, corona radiata and centrum semiovale (white arrowheads) were also noted

    Article Snippet: Integrated PET/MRI was performed on 48 patients with suspected dementia from Jan 2013 to Dec 2014 using simultaneous hybrid PET/MRI scanner (Siemens Biograph mMR; Siemens Healthcare, Erlangen, Germany).

    Techniques: Magnetic Resonance Imaging, Positron Emission Tomography

    D600 PET/CT vs DMI PET/CT: no additional bone lesions were detected by DMI PET/CT compared to standard PET/CT. 61 years-old man with tonsillar cancer who underwent PET/CT for evaluation of response to therapy. DMI images were acquired 47.4 minutes after standard acquisition and 126 minutes after 18 F-FDG injection. A) D600 MIP; B) DMI MIP; C) D600 sagittal fused image; D) DMI sagittal fused image.

    Journal: PLoS ONE

    Article Title: 18F-FDG silicon photomultiplier PET/CT: A pilot study comparing semi-quantitative measurements with standard PET/CT

    doi: 10.1371/journal.pone.0178936

    Figure Lengend Snippet: D600 PET/CT vs DMI PET/CT: no additional bone lesions were detected by DMI PET/CT compared to standard PET/CT. 61 years-old man with tonsillar cancer who underwent PET/CT for evaluation of response to therapy. DMI images were acquired 47.4 minutes after standard acquisition and 126 minutes after 18 F-FDG injection. A) D600 MIP; B) DMI MIP; C) D600 sagittal fused image; D) DMI sagittal fused image.

    Article Snippet: We recently installed a new generation PET/CT scanner (GE Discovery Molecular Insights—DMI PET/CT, GE Healthcare, Waukesha, WI).

    Techniques: Positron Emission Tomography, Injection

    D690 PET/CT vs DMI PET/CT at different time delay between scans. A1) D690 PET; A2) D690 CT; A3) D690 fused image; B1) DMI PET; B2) DMI CT; B3) DMI fused image. Time delay between scans was 18 minutes. C1) D690 PET; C2) D690 CT; C3) D690 fused image; D1) DMI PET; D2) DMI CT; D3) DMI fused image. Time delay between scans was 73.7 minutes.

    Journal: PLoS ONE

    Article Title: 18F-FDG silicon photomultiplier PET/CT: A pilot study comparing semi-quantitative measurements with standard PET/CT

    doi: 10.1371/journal.pone.0178936

    Figure Lengend Snippet: D690 PET/CT vs DMI PET/CT at different time delay between scans. A1) D690 PET; A2) D690 CT; A3) D690 fused image; B1) DMI PET; B2) DMI CT; B3) DMI fused image. Time delay between scans was 18 minutes. C1) D690 PET; C2) D690 CT; C3) D690 fused image; D1) DMI PET; D2) DMI CT; D3) DMI fused image. Time delay between scans was 73.7 minutes.

    Article Snippet: We recently installed a new generation PET/CT scanner (GE Discovery Molecular Insights—DMI PET/CT, GE Healthcare, Waukesha, WI).

    Techniques: Positron Emission Tomography

    Bland-Altman plot analysis. Bland-Altman plot analysis showed an acceptable agreement between scanners for most of the SUV max values registered. There is a significant bias between measurements corresponding to the higher sensitivity of DMI PET/CT. The disagreement between measurements above the 95% CI is related to lesions for which DMI PET/CT registered the greatest increase of SUV max .

    Journal: PLoS ONE

    Article Title: 18F-FDG silicon photomultiplier PET/CT: A pilot study comparing semi-quantitative measurements with standard PET/CT

    doi: 10.1371/journal.pone.0178936

    Figure Lengend Snippet: Bland-Altman plot analysis. Bland-Altman plot analysis showed an acceptable agreement between scanners for most of the SUV max values registered. There is a significant bias between measurements corresponding to the higher sensitivity of DMI PET/CT. The disagreement between measurements above the 95% CI is related to lesions for which DMI PET/CT registered the greatest increase of SUV max .

    Article Snippet: We recently installed a new generation PET/CT scanner (GE Discovery Molecular Insights—DMI PET/CT, GE Healthcare, Waukesha, WI).

    Techniques: Positron Emission Tomography

    D690 PET/CT vs DMI PET/CT: DMI showed an additional focal avid FDG uptake area (black arrow). 46 years-old man with myocardial sarcoidosis who underwent PET/CT for initial staging. DMI images were acquired 39.1 minutes after standard acquisition and 109.2 minutes after 18 F-FDG injection. A) D690 Maximum-intensity-projection (MIP); B) DMI MIP; C) D690 coronal fused image D) DMI coronal fused image.

    Journal: PLoS ONE

    Article Title: 18F-FDG silicon photomultiplier PET/CT: A pilot study comparing semi-quantitative measurements with standard PET/CT

    doi: 10.1371/journal.pone.0178936

    Figure Lengend Snippet: D690 PET/CT vs DMI PET/CT: DMI showed an additional focal avid FDG uptake area (black arrow). 46 years-old man with myocardial sarcoidosis who underwent PET/CT for initial staging. DMI images were acquired 39.1 minutes after standard acquisition and 109.2 minutes after 18 F-FDG injection. A) D690 Maximum-intensity-projection (MIP); B) DMI MIP; C) D690 coronal fused image D) DMI coronal fused image.

    Article Snippet: We recently installed a new generation PET/CT scanner (GE Discovery Molecular Insights—DMI PET/CT, GE Healthcare, Waukesha, WI).

    Techniques: Positron Emission Tomography, Injection

    D690 PET/CT vs DMI PET/CT: DMI showed a significant increase in SUV max (red arrow). 79 years-old man with kidney cancer who performed PET/CT for evaluation of response to therapy. DMI images were acquired 34 minutes after standard PET/CT acquisition and 104 minutes after 18 F-FDG injection. A1) D690 PET; A2) D690 CT; A3) D690 fused image; B1) DMI PET; B2) DMI CT; B3) DMI fused image; C) D690 MIP; D) DMI MIP.

    Journal: PLoS ONE

    Article Title: 18F-FDG silicon photomultiplier PET/CT: A pilot study comparing semi-quantitative measurements with standard PET/CT

    doi: 10.1371/journal.pone.0178936

    Figure Lengend Snippet: D690 PET/CT vs DMI PET/CT: DMI showed a significant increase in SUV max (red arrow). 79 years-old man with kidney cancer who performed PET/CT for evaluation of response to therapy. DMI images were acquired 34 minutes after standard PET/CT acquisition and 104 minutes after 18 F-FDG injection. A1) D690 PET; A2) D690 CT; A3) D690 fused image; B1) DMI PET; B2) DMI CT; B3) DMI fused image; C) D690 MIP; D) DMI MIP.

    Article Snippet: We recently installed a new generation PET/CT scanner (GE Discovery Molecular Insights—DMI PET/CT, GE Healthcare, Waukesha, WI).

    Techniques: Positron Emission Tomography, Injection

    Lesional SUV max measured twice over time. Recognizable increase in lesion SUV max in DMI PET/CT. Partial correlation and linear regression were used to exclude time as independent variable between different SUV max values.

    Journal: PLoS ONE

    Article Title: 18F-FDG silicon photomultiplier PET/CT: A pilot study comparing semi-quantitative measurements with standard PET/CT

    doi: 10.1371/journal.pone.0178936

    Figure Lengend Snippet: Lesional SUV max measured twice over time. Recognizable increase in lesion SUV max in DMI PET/CT. Partial correlation and linear regression were used to exclude time as independent variable between different SUV max values.

    Article Snippet: We recently installed a new generation PET/CT scanner (GE Discovery Molecular Insights—DMI PET/CT, GE Healthcare, Waukesha, WI).

    Techniques: Positron Emission Tomography

    (a) Axial CT, (b) fused FDG PET/CT, and (c) fused FDHT PET/CT images in an 81-year-old patient with an FDHT-predominant phenotype. A ground-glass lesion in the left sacral ala (white arrowheads) has an attenuation of 352 HU and uptake on FDG (SUV max ,

    Journal:

    Article Title: Bone Metastases in Castration-Resistant Prostate Cancer: Associations between Morphologic CT Patterns, Glycolytic Activity, and Androgen Receptor Expression on PET and Overall Survival

    doi: 10.1148/radiol.13130625

    Figure Lengend Snippet: (a) Axial CT, (b) fused FDG PET/CT, and (c) fused FDHT PET/CT images in an 81-year-old patient with an FDHT-predominant phenotype. A ground-glass lesion in the left sacral ala (white arrowheads) has an attenuation of 352 HU and uptake on FDG (SUV max ,

    Article Snippet: FDG and FDHT PET/CT examinations were performed with a PET/CT scanner (Discovery STE; GE Healthcare, Milwaukee, Wis).

    Techniques: Positron Emission Tomography

    (a) Axial CT, (b) fused FDG PET/CT, and (c) fused FDHT PET/CT images in a 66-year-old patient with a predominantly lytic lesion in the sacrum (arrow) that shows marked FDG uptake (SUV max , 16) but no abnormal FDHT uptake on PET images.

    Journal:

    Article Title: Bone Metastases in Castration-Resistant Prostate Cancer: Associations between Morphologic CT Patterns, Glycolytic Activity, and Androgen Receptor Expression on PET and Overall Survival

    doi: 10.1148/radiol.13130625

    Figure Lengend Snippet: (a) Axial CT, (b) fused FDG PET/CT, and (c) fused FDHT PET/CT images in a 66-year-old patient with a predominantly lytic lesion in the sacrum (arrow) that shows marked FDG uptake (SUV max , 16) but no abnormal FDHT uptake on PET images.

    Article Snippet: FDG and FDHT PET/CT examinations were performed with a PET/CT scanner (Discovery STE; GE Healthcare, Milwaukee, Wis).

    Techniques: Positron Emission Tomography

    (a) Axial CT, (b) fused FDG PET/CT, and (c) fused FDHT PET/CT images in an 81-year-old patient with an FDHT-predominant phenotype. A ground-glass lesion in the left sacral ala (white arrowheads) has an attenuation of 352 HU and uptake on FDG (SUV max ,

    Journal:

    Article Title: Bone Metastases in Castration-Resistant Prostate Cancer: Associations between Morphologic CT Patterns, Glycolytic Activity, and Androgen Receptor Expression on PET and Overall Survival

    doi: 10.1148/radiol.13130625

    Figure Lengend Snippet: (a) Axial CT, (b) fused FDG PET/CT, and (c) fused FDHT PET/CT images in an 81-year-old patient with an FDHT-predominant phenotype. A ground-glass lesion in the left sacral ala (white arrowheads) has an attenuation of 352 HU and uptake on FDG (SUV max ,

    Article Snippet: FDG and FDHT PET/CT examinations were performed with a PET/CT scanner (Discovery STE; GE Healthcare, Milwaukee, Wis).

    Techniques: Positron Emission Tomography

    Scatterplots show the relationships of mean Hounsfield units to SUV max at FDG PET and SUV max at FDHT PET for each bone lesion for (a, b) reader 1 and (c, d) reader 2.

    Journal:

    Article Title: Bone Metastases in Castration-Resistant Prostate Cancer: Associations between Morphologic CT Patterns, Glycolytic Activity, and Androgen Receptor Expression on PET and Overall Survival

    doi: 10.1148/radiol.13130625

    Figure Lengend Snippet: Scatterplots show the relationships of mean Hounsfield units to SUV max at FDG PET and SUV max at FDHT PET for each bone lesion for (a, b) reader 1 and (c, d) reader 2.

    Article Snippet: FDG and FDHT PET/CT examinations were performed with a PET/CT scanner (Discovery STE; GE Healthcare, Milwaukee, Wis).

    Techniques: Positron Emission Tomography

    Scatterplots show the relationships of mean Hounsfield units to SUV max at FDG PET and SUV max at FDHT PET for each bone lesion for (a, b) reader 1 and (c, d) reader 2.

    Journal:

    Article Title: Bone Metastases in Castration-Resistant Prostate Cancer: Associations between Morphologic CT Patterns, Glycolytic Activity, and Androgen Receptor Expression on PET and Overall Survival

    doi: 10.1148/radiol.13130625

    Figure Lengend Snippet: Scatterplots show the relationships of mean Hounsfield units to SUV max at FDG PET and SUV max at FDHT PET for each bone lesion for (a, b) reader 1 and (c, d) reader 2.

    Article Snippet: FDG and FDHT PET/CT examinations were performed with a PET/CT scanner (Discovery STE; GE Healthcare, Milwaukee, Wis).

    Techniques: Positron Emission Tomography

    Morphologic Patterns of Lesions at CT and Correlation with FDG and FDHT Uptake at PET

    Journal:

    Article Title: Bone Metastases in Castration-Resistant Prostate Cancer: Associations between Morphologic CT Patterns, Glycolytic Activity, and Androgen Receptor Expression on PET and Overall Survival

    doi: 10.1148/radiol.13130625

    Figure Lengend Snippet: Morphologic Patterns of Lesions at CT and Correlation with FDG and FDHT Uptake at PET

    Article Snippet: FDG and FDHT PET/CT examinations were performed with a PET/CT scanner (Discovery STE; GE Healthcare, Milwaukee, Wis).

    Techniques: Positron Emission Tomography

    (a) Axial CT, (b) fused FDG PET/CT, and (c) fused FDHT PET/CT images in a 66-year-old patient with a predominantly lytic lesion in the sacrum (arrow) that shows marked FDG uptake (SUV max , 16) but no abnormal FDHT uptake on PET images.

    Journal:

    Article Title: Bone Metastases in Castration-Resistant Prostate Cancer: Associations between Morphologic CT Patterns, Glycolytic Activity, and Androgen Receptor Expression on PET and Overall Survival

    doi: 10.1148/radiol.13130625

    Figure Lengend Snippet: (a) Axial CT, (b) fused FDG PET/CT, and (c) fused FDHT PET/CT images in a 66-year-old patient with a predominantly lytic lesion in the sacrum (arrow) that shows marked FDG uptake (SUV max , 16) but no abnormal FDHT uptake on PET images.

    Article Snippet: FDG and FDHT PET/CT examinations were performed with a PET/CT scanner (Discovery STE; GE Healthcare, Milwaukee, Wis).

    Techniques: Positron Emission Tomography

    Scatterplots show the relationships of mean Hounsfield units to SUV max at FDG PET and SUV max at FDHT PET for each bone lesion for (a, b) reader 1 and (c, d) reader 2.

    Journal:

    Article Title: Bone Metastases in Castration-Resistant Prostate Cancer: Associations between Morphologic CT Patterns, Glycolytic Activity, and Androgen Receptor Expression on PET and Overall Survival

    doi: 10.1148/radiol.13130625

    Figure Lengend Snippet: Scatterplots show the relationships of mean Hounsfield units to SUV max at FDG PET and SUV max at FDHT PET for each bone lesion for (a, b) reader 1 and (c, d) reader 2.

    Article Snippet: FDG and FDHT PET/CT examinations were performed with a PET/CT scanner (Discovery STE; GE Healthcare, Milwaukee, Wis).

    Techniques: Positron Emission Tomography

    (a) Axial CT, (b) fused FDG PET/CT, and (c) fused FDHT PET/CT images in a 66-year-old patient with a predominantly lytic lesion in the sacrum (arrow) that shows marked FDG uptake (SUV max , 16) but no abnormal FDHT uptake on PET images.

    Journal:

    Article Title: Bone Metastases in Castration-Resistant Prostate Cancer: Associations between Morphologic CT Patterns, Glycolytic Activity, and Androgen Receptor Expression on PET and Overall Survival

    doi: 10.1148/radiol.13130625

    Figure Lengend Snippet: (a) Axial CT, (b) fused FDG PET/CT, and (c) fused FDHT PET/CT images in a 66-year-old patient with a predominantly lytic lesion in the sacrum (arrow) that shows marked FDG uptake (SUV max , 16) but no abnormal FDHT uptake on PET images.

    Article Snippet: FDG and FDHT PET/CT examinations were performed with a PET/CT scanner (Discovery STE; GE Healthcare, Milwaukee, Wis).

    Techniques: Positron Emission Tomography

    Scatterplots show the relationships of mean Hounsfield units to SUV max at FDG PET and SUV max at FDHT PET for each bone lesion for (a, b) reader 1 and (c, d) reader 2.

    Journal:

    Article Title: Bone Metastases in Castration-Resistant Prostate Cancer: Associations between Morphologic CT Patterns, Glycolytic Activity, and Androgen Receptor Expression on PET and Overall Survival

    doi: 10.1148/radiol.13130625

    Figure Lengend Snippet: Scatterplots show the relationships of mean Hounsfield units to SUV max at FDG PET and SUV max at FDHT PET for each bone lesion for (a, b) reader 1 and (c, d) reader 2.

    Article Snippet: FDG and FDHT PET/CT examinations were performed with a PET/CT scanner (Discovery STE; GE Healthcare, Milwaukee, Wis).

    Techniques: Positron Emission Tomography

    (a) Axial CT, (b) fused FDG PET/CT, and (c) fused FDHT PET/CT images in an 81-year-old patient with an FDHT-predominant phenotype. A ground-glass lesion in the left sacral ala (white arrowheads) has an attenuation of 352 HU and uptake on FDG (SUV max ,

    Journal:

    Article Title: Bone Metastases in Castration-Resistant Prostate Cancer: Associations between Morphologic CT Patterns, Glycolytic Activity, and Androgen Receptor Expression on PET and Overall Survival

    doi: 10.1148/radiol.13130625

    Figure Lengend Snippet: (a) Axial CT, (b) fused FDG PET/CT, and (c) fused FDHT PET/CT images in an 81-year-old patient with an FDHT-predominant phenotype. A ground-glass lesion in the left sacral ala (white arrowheads) has an attenuation of 352 HU and uptake on FDG (SUV max ,

    Article Snippet: FDG and FDHT PET/CT examinations were performed with a PET/CT scanner (Discovery STE; GE Healthcare, Milwaukee, Wis).

    Techniques: Positron Emission Tomography

    Comparison of lymph node staging (N-stage) on pre-transurethral resection ( a , b , c ) and post-chemotherapy 11 C-acetate PET/MRI ( d , e , f ) in patient number 12, the same patient as in Fig. 4 . 17 mm right obturator lymph node ( a - white arrow on T2-weighted image) demonstrated increased diffusion signal restriction ( b - b value 800 s/mm 2 trace diffusion weighted image) and increased 11 C-acetate uptake ( c - PET fused with T2-weighted image, SUV is scaled from 0.0 to 3.5) suggestive of lymph node metastasis. On post-chemotherapy 11 C-acetate PET/MRI, lymph node decreased in size and measured 4 mm ( d - green arrow on T2-weighted image) with increased diffusion signal ( e - b value 800 s/mm 2 trace diffusion weighted image) and increased 11 C-acetate uptake ( f - PET fused with T2-weighted image, SUV is scaled from 0.0 to 3.0). SUVmax values of the lymph node on the pre-transurethral resection ( c ) and post-chemotherapy 11 C-acetate PET/MRI ( f ) were 3.4 and 2.8, respectively. The lymph node was confirmed to be lymph node metastasis measuring 3 mm on extended pelvic lymph node dissection, thus the findings of 11 C-acetate PET/MRI were considered as true positive

    Journal: Cancer Imaging

    Article Title: 11C-acetate PET/MRI in bladder cancer staging and treatment response evaluation to neoadjuvant chemotherapy: a prospective multicenter study (ACEBIB trial)

    doi: 10.1186/s40644-018-0158-4

    Figure Lengend Snippet: Comparison of lymph node staging (N-stage) on pre-transurethral resection ( a , b , c ) and post-chemotherapy 11 C-acetate PET/MRI ( d , e , f ) in patient number 12, the same patient as in Fig. 4 . 17 mm right obturator lymph node ( a - white arrow on T2-weighted image) demonstrated increased diffusion signal restriction ( b - b value 800 s/mm 2 trace diffusion weighted image) and increased 11 C-acetate uptake ( c - PET fused with T2-weighted image, SUV is scaled from 0.0 to 3.5) suggestive of lymph node metastasis. On post-chemotherapy 11 C-acetate PET/MRI, lymph node decreased in size and measured 4 mm ( d - green arrow on T2-weighted image) with increased diffusion signal ( e - b value 800 s/mm 2 trace diffusion weighted image) and increased 11 C-acetate uptake ( f - PET fused with T2-weighted image, SUV is scaled from 0.0 to 3.0). SUVmax values of the lymph node on the pre-transurethral resection ( c ) and post-chemotherapy 11 C-acetate PET/MRI ( f ) were 3.4 and 2.8, respectively. The lymph node was confirmed to be lymph node metastasis measuring 3 mm on extended pelvic lymph node dissection, thus the findings of 11 C-acetate PET/MRI were considered as true positive

    Article Snippet: All patients were imaged with an Ingenuity TF PET/MRI scanner (Philips Medical Systems, Cleveland, OH).

    Techniques: Positron Emission Tomography, Magnetic Resonance Imaging, Diffusion-based Assay, Dissection

    Comparison of lymph node staging (N-stage) on pre-transurethral resection ( a , b , c ) and post-chemotherapy 11 C-acetate PET/MRI ( d , e , f ) in a 66-year-old male (patient number 6 in primary imaging). 8 mm retroaortic lymph node ( d - white arrow on T2-weighted image) demonstrated increased diffusion signal restriction ( b - b value 800 s/mm 2 trace diffusion weighted image), and increased 11 C-acetate uptake ( c - PET fused with T2-weighted image, SUV is scaled from 0.0 to 2.8) suggestive of lymph node metastasis. On post-chemotherapy 11 C-acetate PET/MRI, lymph did not decrease in size and measured 7 mm ( d - green arrow on T2-weighted image) with increased diffusion signal ( e - b value 800 s/mm 2 trace diffusion weighted image) and increased 11 C-acetate uptake ( f - PET fused with T2-weighted image, SUV is scaled from 0.0 to 2.8). SUVmax values of the lymph node on the pre-transurethral resection ( c ) and post-chemotherapy 11 C-acetate PET/MRI ( f ) was 1.7, 1.3, respectively. No lymph node metastases were found on extended pelvic lymph node dissection, thus the findings of 11 C-acetate PET/MRI were considered as false positive

    Journal: Cancer Imaging

    Article Title: 11C-acetate PET/MRI in bladder cancer staging and treatment response evaluation to neoadjuvant chemotherapy: a prospective multicenter study (ACEBIB trial)

    doi: 10.1186/s40644-018-0158-4

    Figure Lengend Snippet: Comparison of lymph node staging (N-stage) on pre-transurethral resection ( a , b , c ) and post-chemotherapy 11 C-acetate PET/MRI ( d , e , f ) in a 66-year-old male (patient number 6 in primary imaging). 8 mm retroaortic lymph node ( d - white arrow on T2-weighted image) demonstrated increased diffusion signal restriction ( b - b value 800 s/mm 2 trace diffusion weighted image), and increased 11 C-acetate uptake ( c - PET fused with T2-weighted image, SUV is scaled from 0.0 to 2.8) suggestive of lymph node metastasis. On post-chemotherapy 11 C-acetate PET/MRI, lymph did not decrease in size and measured 7 mm ( d - green arrow on T2-weighted image) with increased diffusion signal ( e - b value 800 s/mm 2 trace diffusion weighted image) and increased 11 C-acetate uptake ( f - PET fused with T2-weighted image, SUV is scaled from 0.0 to 2.8). SUVmax values of the lymph node on the pre-transurethral resection ( c ) and post-chemotherapy 11 C-acetate PET/MRI ( f ) was 1.7, 1.3, respectively. No lymph node metastases were found on extended pelvic lymph node dissection, thus the findings of 11 C-acetate PET/MRI were considered as false positive

    Article Snippet: All patients were imaged with an Ingenuity TF PET/MRI scanner (Philips Medical Systems, Cleveland, OH).

    Techniques: Positron Emission Tomography, Magnetic Resonance Imaging, Imaging, Diffusion-based Assay, Dissection

    Flow chart of the study protocol. The trial consisted of two phases: in phase 1 accuracy of 11 C-acetate PET/MRI was evaluated on 15 treatment naive patients before any intervention of primary tumor. In phase 2 treatment response to Neoadjuvant chemotherapy (NAC) was evaluated in 5 patients undergoing 11 C-acetate PET/MRI after transurethral resection of bladder tumor (TUR-BT) and neoadjuvant chemotherapy. 2 patients participated in both phases of the study. In total, 18 patients were enrolled

    Journal: Cancer Imaging

    Article Title: 11C-acetate PET/MRI in bladder cancer staging and treatment response evaluation to neoadjuvant chemotherapy: a prospective multicenter study (ACEBIB trial)

    doi: 10.1186/s40644-018-0158-4

    Figure Lengend Snippet: Flow chart of the study protocol. The trial consisted of two phases: in phase 1 accuracy of 11 C-acetate PET/MRI was evaluated on 15 treatment naive patients before any intervention of primary tumor. In phase 2 treatment response to Neoadjuvant chemotherapy (NAC) was evaluated in 5 patients undergoing 11 C-acetate PET/MRI after transurethral resection of bladder tumor (TUR-BT) and neoadjuvant chemotherapy. 2 patients participated in both phases of the study. In total, 18 patients were enrolled

    Article Snippet: All patients were imaged with an Ingenuity TF PET/MRI scanner (Philips Medical Systems, Cleveland, OH).

    Techniques: Flow Cytometry, Positron Emission Tomography, Magnetic Resonance Imaging

    Comparison of local bladder cancer staging (T-stage) on pre-transurethral resection ( a , b , c ) and post-chemotherapy 11 C-acetate PET/MRI ( d , e , f ) in a 63-year-old male patient (number 12 in primary imaging). A heterogenous lobulated mass on the right side of mid-line with extension (white arrow) to perivesical fat seen on T2-weighted image ( a ), an area of increased diffusion signal restriction (white arrow) beyond the bladder wall ( b - b value 800 s/mm 2 trace diffusion weighted image), an associated right sided hydroureter, and increased 11 C-acetate uptake ( c - PET fused with T2-weighted image, SUV is scaled from 0.0 to 3.5) suggestive of T3 stage. On post-chemotherapy 11 C-acetate PET/MRI, residual abnormal wall thickening (green arrow) on T2-weighted image ( d ) and diffusion signal restriction extending to perivesical fat ( e - b value 800 s/mm 2 trace diffusion weighted image) was presented suggestive of T3 stage. The final cystectomy specimen revealed stage T2, thus the findings of 11 C-acetate PET/MRI were considered as true positive for muscle invasion

    Journal: Cancer Imaging

    Article Title: 11C-acetate PET/MRI in bladder cancer staging and treatment response evaluation to neoadjuvant chemotherapy: a prospective multicenter study (ACEBIB trial)

    doi: 10.1186/s40644-018-0158-4

    Figure Lengend Snippet: Comparison of local bladder cancer staging (T-stage) on pre-transurethral resection ( a , b , c ) and post-chemotherapy 11 C-acetate PET/MRI ( d , e , f ) in a 63-year-old male patient (number 12 in primary imaging). A heterogenous lobulated mass on the right side of mid-line with extension (white arrow) to perivesical fat seen on T2-weighted image ( a ), an area of increased diffusion signal restriction (white arrow) beyond the bladder wall ( b - b value 800 s/mm 2 trace diffusion weighted image), an associated right sided hydroureter, and increased 11 C-acetate uptake ( c - PET fused with T2-weighted image, SUV is scaled from 0.0 to 3.5) suggestive of T3 stage. On post-chemotherapy 11 C-acetate PET/MRI, residual abnormal wall thickening (green arrow) on T2-weighted image ( d ) and diffusion signal restriction extending to perivesical fat ( e - b value 800 s/mm 2 trace diffusion weighted image) was presented suggestive of T3 stage. The final cystectomy specimen revealed stage T2, thus the findings of 11 C-acetate PET/MRI were considered as true positive for muscle invasion

    Article Snippet: All patients were imaged with an Ingenuity TF PET/MRI scanner (Philips Medical Systems, Cleveland, OH).

    Techniques: Positron Emission Tomography, Magnetic Resonance Imaging, Imaging, Diffusion-based Assay

    Pre-transurethral resection 11 C-acetate PET/MRI ( a , b , c ) in a 75-year old male patient (number 3 in primary imaging) showing a heterogenous lobulated mass on the left side of mid-line without extension (white arrow) to perivesical fat on T2-weighted image ( a ) or an area of increased diffusion signal restriction (white arrow) extending beyond the bladder wall ( b - b value 800 s/mm 2 trace diffusion weighted image). The lesion had increased 11 C-acetate uptake ( c - PET fused with T2-weighted image, SUV is scaled from 0.0 to 3.2). The imaging findings were suggestive of T1 stage. The transurethral resection of bladder tumor specimen revealed stage T1, thus the findings of 11 C-acetate PET/MRI correctly staged the tumor

    Journal: Cancer Imaging

    Article Title: 11C-acetate PET/MRI in bladder cancer staging and treatment response evaluation to neoadjuvant chemotherapy: a prospective multicenter study (ACEBIB trial)

    doi: 10.1186/s40644-018-0158-4

    Figure Lengend Snippet: Pre-transurethral resection 11 C-acetate PET/MRI ( a , b , c ) in a 75-year old male patient (number 3 in primary imaging) showing a heterogenous lobulated mass on the left side of mid-line without extension (white arrow) to perivesical fat on T2-weighted image ( a ) or an area of increased diffusion signal restriction (white arrow) extending beyond the bladder wall ( b - b value 800 s/mm 2 trace diffusion weighted image). The lesion had increased 11 C-acetate uptake ( c - PET fused with T2-weighted image, SUV is scaled from 0.0 to 3.2). The imaging findings were suggestive of T1 stage. The transurethral resection of bladder tumor specimen revealed stage T1, thus the findings of 11 C-acetate PET/MRI correctly staged the tumor

    Article Snippet: All patients were imaged with an Ingenuity TF PET/MRI scanner (Philips Medical Systems, Cleveland, OH).

    Techniques: Positron Emission Tomography, Magnetic Resonance Imaging, Imaging, Diffusion-based Assay

    A 46-year-old woman with breast cancer. A μ-map of FDG PET/MR mammography ( a ) show body contour artifact with missing dorsal body contour including both lungs causing failure of attenuation correction on an axial PET image ( b )

    Journal:

    Article Title: Potential Clinical Applications of 18F-Fluorodeoxyglucose Positron Emission Tomography/Magnetic Resonance Mammography in Breast Cancer

    doi: 10.1007/s13139-016-0446-5

    Figure Lengend Snippet: A 46-year-old woman with breast cancer. A μ-map of FDG PET/MR mammography ( a ) show body contour artifact with missing dorsal body contour including both lungs causing failure of attenuation correction on an axial PET image ( b )

    Article Snippet: Botsikas et al. [ ], who evaluated primary lesions in 58 patients with breast cancer using a sequential whole-body FDG PET/MR scanner (Philips Ingenuity TF PET/MR; Philips Healthcare, Best, The Netherlands), reported that sensitivity for primary cancers with MR breast imaging and fused PET/MR breast imaging was 100 and 77 %, respectively, and specificity for MR breast imaging and fused PET/MR breast imaging was 67 and 100 %.

    Techniques: Positron Emission Tomography

    Comparison between FDG uptake and the ADC in a 52-year-old woman with a 3.5-cm invasive ductal carcinoma. a Region of interest for measuring SUVmax is drawn on the axial PET/MR mammography (SUVmax , 8.63). b Contrast-enhanced T1 fs GRE subtraction image showed a heterogeneous enhancing mass. c DWI ( b value, 800 mm2 /s) shows high signal intensity within the tumor. d For measurement of the mean ADC, a region of interest is manually drawn within a mass lesion on an ADC map (mean ADC, 0.825 × 10−3 mm2 /s)

    Journal:

    Article Title: Potential Clinical Applications of 18F-Fluorodeoxyglucose Positron Emission Tomography/Magnetic Resonance Mammography in Breast Cancer

    doi: 10.1007/s13139-016-0446-5

    Figure Lengend Snippet: Comparison between FDG uptake and the ADC in a 52-year-old woman with a 3.5-cm invasive ductal carcinoma. a Region of interest for measuring SUVmax is drawn on the axial PET/MR mammography (SUVmax , 8.63). b Contrast-enhanced T1 fs GRE subtraction image showed a heterogeneous enhancing mass. c DWI ( b value, 800 mm2 /s) shows high signal intensity within the tumor. d For measurement of the mean ADC, a region of interest is manually drawn within a mass lesion on an ADC map (mean ADC, 0.825 × 10−3 mm2 /s)

    Article Snippet: Botsikas et al. [ ], who evaluated primary lesions in 58 patients with breast cancer using a sequential whole-body FDG PET/MR scanner (Philips Ingenuity TF PET/MR; Philips Healthcare, Best, The Netherlands), reported that sensitivity for primary cancers with MR breast imaging and fused PET/MR breast imaging was 100 and 77 %, respectively, and specificity for MR breast imaging and fused PET/MR breast imaging was 67 and 100 %.

    Techniques: Positron Emission Tomography

    A 49-year-old woman with an invasive carcinoma and one axillary lymph node metastasis. Increased FDG uptake is shown in the upper outer quadrant of the right breast on FDG PET ( a ) and FDG/T1 fs GRE PET/MR mammography ( b ). Axillary lymph node ( arrow ) with faint FDG uptake and focal cortical thickening is seen in the right axilla on FDG PET ( c ) and FDG/T1 fs GRE PET/MR mammography ( d )

    Journal:

    Article Title: Potential Clinical Applications of 18F-Fluorodeoxyglucose Positron Emission Tomography/Magnetic Resonance Mammography in Breast Cancer

    doi: 10.1007/s13139-016-0446-5

    Figure Lengend Snippet: A 49-year-old woman with an invasive carcinoma and one axillary lymph node metastasis. Increased FDG uptake is shown in the upper outer quadrant of the right breast on FDG PET ( a ) and FDG/T1 fs GRE PET/MR mammography ( b ). Axillary lymph node ( arrow ) with faint FDG uptake and focal cortical thickening is seen in the right axilla on FDG PET ( c ) and FDG/T1 fs GRE PET/MR mammography ( d )

    Article Snippet: Botsikas et al. [ ], who evaluated primary lesions in 58 patients with breast cancer using a sequential whole-body FDG PET/MR scanner (Philips Ingenuity TF PET/MR; Philips Healthcare, Best, The Netherlands), reported that sensitivity for primary cancers with MR breast imaging and fused PET/MR breast imaging was 100 and 77 %, respectively, and specificity for MR breast imaging and fused PET/MR breast imaging was 67 and 100 %.

    Techniques: Positron Emission Tomography

    A 52-year-old woman with three invasive ductal carcinomas in the left breast. a The MIP of a contrast-enhanced T1 fs GRE subtraction image shows three enhancing masses in the left breast. b FDG/T1 fs GRE PET/MR mammography shows the largest hypermetabolic mass with a low ADC value (0.962 × 10−3 mm2 /s) ( c ) and a washout enhancement ( d , e ). f , g FDG/T1 fs GRE PET/MR mammography shows two enhancing masses, one with moderate FDG uptake and one with no FDG uptake. h The smallest enhancing mass without FDG uptake is shown as a washout enhancement pattern on the color-coded map

    Journal:

    Article Title: Potential Clinical Applications of 18F-Fluorodeoxyglucose Positron Emission Tomography/Magnetic Resonance Mammography in Breast Cancer

    doi: 10.1007/s13139-016-0446-5

    Figure Lengend Snippet: A 52-year-old woman with three invasive ductal carcinomas in the left breast. a The MIP of a contrast-enhanced T1 fs GRE subtraction image shows three enhancing masses in the left breast. b FDG/T1 fs GRE PET/MR mammography shows the largest hypermetabolic mass with a low ADC value (0.962 × 10−3 mm2 /s) ( c ) and a washout enhancement ( d , e ). f , g FDG/T1 fs GRE PET/MR mammography shows two enhancing masses, one with moderate FDG uptake and one with no FDG uptake. h The smallest enhancing mass without FDG uptake is shown as a washout enhancement pattern on the color-coded map

    Article Snippet: Botsikas et al. [ ], who evaluated primary lesions in 58 patients with breast cancer using a sequential whole-body FDG PET/MR scanner (Philips Ingenuity TF PET/MR; Philips Healthcare, Best, The Netherlands), reported that sensitivity for primary cancers with MR breast imaging and fused PET/MR breast imaging was 100 and 77 %, respectively, and specificity for MR breast imaging and fused PET/MR breast imaging was 67 and 100 %.

    Techniques: Positron Emission Tomography

    Images of whole-body FDG PET/MR scan and PET/MR mammography obtained with a dedicated four-channel PET/MR breast coil in a 41-year-old woman with invasive ductal carcinoma. A spiculated enhancing mass with strong FDG uptake, a low apparent diffusion coefficient (ADC) value, and a washout enhancement pattern is seen at the 12 o’clock position in the left breast on FDG PET ( a ), FDG/ADC PET/ mammography ( b ), color-coded map of the maximum slope of enhancement ( c ), and FDG/T1 fat-saturated gradient-echo (fs GRE) PET/MR mammography ( d ). Pelvic FDG/T1 and whole body FDG/T2 PET/MR images ( e , f ) show moderate FDG uptake in the left iliac bone suspicious for bone metastasis ( arrows )

    Journal:

    Article Title: Potential Clinical Applications of 18F-Fluorodeoxyglucose Positron Emission Tomography/Magnetic Resonance Mammography in Breast Cancer

    doi: 10.1007/s13139-016-0446-5

    Figure Lengend Snippet: Images of whole-body FDG PET/MR scan and PET/MR mammography obtained with a dedicated four-channel PET/MR breast coil in a 41-year-old woman with invasive ductal carcinoma. A spiculated enhancing mass with strong FDG uptake, a low apparent diffusion coefficient (ADC) value, and a washout enhancement pattern is seen at the 12 o’clock position in the left breast on FDG PET ( a ), FDG/ADC PET/ mammography ( b ), color-coded map of the maximum slope of enhancement ( c ), and FDG/T1 fat-saturated gradient-echo (fs GRE) PET/MR mammography ( d ). Pelvic FDG/T1 and whole body FDG/T2 PET/MR images ( e , f ) show moderate FDG uptake in the left iliac bone suspicious for bone metastasis ( arrows )

    Article Snippet: Botsikas et al. [ ], who evaluated primary lesions in 58 patients with breast cancer using a sequential whole-body FDG PET/MR scanner (Philips Ingenuity TF PET/MR; Philips Healthcare, Best, The Netherlands), reported that sensitivity for primary cancers with MR breast imaging and fused PET/MR breast imaging was 100 and 77 %, respectively, and specificity for MR breast imaging and fused PET/MR breast imaging was 67 and 100 %.

    Techniques: Positron Emission Tomography, Diffusion-based Assay

    A 72-year-old woman with an invasive carcinoma. Baseline FDG PET ( a ) and FDG/ADC PET/MR mammography ( b ). After the first cycle of neoadjuvant chemotherapy, a significant reduction in tumor FDG uptake from an SUVmax of 13.8 to an SUVmax of 6.4 without a change in the ADC is seen on FDG PET ( c ) and FDG/ADC PET/MR mammography ( d ). Histopathology at completion of chemotherapy showed no residual disease in the tumor bed

    Journal:

    Article Title: Potential Clinical Applications of 18F-Fluorodeoxyglucose Positron Emission Tomography/Magnetic Resonance Mammography in Breast Cancer

    doi: 10.1007/s13139-016-0446-5

    Figure Lengend Snippet: A 72-year-old woman with an invasive carcinoma. Baseline FDG PET ( a ) and FDG/ADC PET/MR mammography ( b ). After the first cycle of neoadjuvant chemotherapy, a significant reduction in tumor FDG uptake from an SUVmax of 13.8 to an SUVmax of 6.4 without a change in the ADC is seen on FDG PET ( c ) and FDG/ADC PET/MR mammography ( d ). Histopathology at completion of chemotherapy showed no residual disease in the tumor bed

    Article Snippet: Botsikas et al. [ ], who evaluated primary lesions in 58 patients with breast cancer using a sequential whole-body FDG PET/MR scanner (Philips Ingenuity TF PET/MR; Philips Healthcare, Best, The Netherlands), reported that sensitivity for primary cancers with MR breast imaging and fused PET/MR breast imaging was 100 and 77 %, respectively, and specificity for MR breast imaging and fused PET/MR breast imaging was 67 and 100 %.

    Techniques: Positron Emission Tomography, Histopathology

    Schematic process for detection of CTCs on a cell microarray chip. (A) Human leukocytes/carcinoma cells are dispersed on a cell microarray chip, followed by 15 minutes' standing to allow the cells to settle down into the microchambers, and are then stained with fluorescence-labeled antibodies against carcinoma cells. (B) Fluorescence-positive CTCs are detected by using a microarray scanner with a confocal fluorescence laser. (C) The target CTCs are analyzed quantitatively at the single-cell level.

    Journal: PLoS ONE

    Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

    doi: 10.1371/journal.pone.0032370

    Figure Lengend Snippet: Schematic process for detection of CTCs on a cell microarray chip. (A) Human leukocytes/carcinoma cells are dispersed on a cell microarray chip, followed by 15 minutes' standing to allow the cells to settle down into the microchambers, and are then stained with fluorescence-labeled antibodies against carcinoma cells. (B) Fluorescence-positive CTCs are detected by using a microarray scanner with a confocal fluorescence laser. (C) The target CTCs are analyzed quantitatively at the single-cell level.

    Article Snippet: Each cell microarray chip was scanned for 20 min with a confocal laser-based fluorescence microarray scanner, CRBIO IIe (Hitachi Software Engineering Co., Ltd., Tokyo, Japan).

    Techniques: Microarray, Chromatin Immunoprecipitation, Staining, Fluorescence, Labeling

    Construction of a cell microarray chip. (A) Photo of an actual cell microarray chip. (B, C) SEM images of a cell microarray chip. The cell microarray chip comprises 20,944 microchambers in a plastic slide on a glass slide. The cell microarray chip has 112 (14×8) clusters, each having 187 microchambers. (D) Each microchamber is 105 µm in upper diameter, 50 µm in depth, and has the shape of a frustum with a 68-µm diameter flat bottom for the accommodation of leukocytes as a monolayer.

    Journal: PLoS ONE

    Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

    doi: 10.1371/journal.pone.0032370

    Figure Lengend Snippet: Construction of a cell microarray chip. (A) Photo of an actual cell microarray chip. (B, C) SEM images of a cell microarray chip. The cell microarray chip comprises 20,944 microchambers in a plastic slide on a glass slide. The cell microarray chip has 112 (14×8) clusters, each having 187 microchambers. (D) Each microchamber is 105 µm in upper diameter, 50 µm in depth, and has the shape of a frustum with a 68-µm diameter flat bottom for the accommodation of leukocytes as a monolayer.

    Article Snippet: Each cell microarray chip was scanned for 20 min with a confocal laser-based fluorescence microarray scanner, CRBIO IIe (Hitachi Software Engineering Co., Ltd., Tokyo, Japan).

    Techniques: Microarray, Chromatin Immunoprecipitation

    Scanned images of double-stained cells. (A–L) Cultured T lymphoblastoid leukemia (A–F) and leukocytes isolated from whole blood (F–L) were stained with PE-labeled anti-cytokeratin monoclonal antibody (A, G) and APC-labeled anti-EpCAM monoclonal antibody (C, I). (E, K) Merged images identify doubly-positive carcinoma cells in each panel. Magnified views of the boxed regions (B, D, F, H, J, L). Scatter-plot analysis of 3 cluster areas representing 561 microchambers in a cell microarray chip ( Fig. 9M ).

    Journal: PLoS ONE

    Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

    doi: 10.1371/journal.pone.0032370

    Figure Lengend Snippet: Scanned images of double-stained cells. (A–L) Cultured T lymphoblastoid leukemia (A–F) and leukocytes isolated from whole blood (F–L) were stained with PE-labeled anti-cytokeratin monoclonal antibody (A, G) and APC-labeled anti-EpCAM monoclonal antibody (C, I). (E, K) Merged images identify doubly-positive carcinoma cells in each panel. Magnified views of the boxed regions (B, D, F, H, J, L). Scatter-plot analysis of 3 cluster areas representing 561 microchambers in a cell microarray chip ( Fig. 9M ).

    Article Snippet: Each cell microarray chip was scanned for 20 min with a confocal laser-based fluorescence microarray scanner, CRBIO IIe (Hitachi Software Engineering Co., Ltd., Tokyo, Japan).

    Techniques: Staining, Cell Culture, Isolation, Labeling, Microarray, Chromatin Immunoprecipitation

    Detection of carcinoma cells among cultured T lymphoblastoid leukemia on a cell microarray chip. (A–I) Scanned images of leukocytes/carcinoma cells on a cell microarray chip obtained with the microarray scanner. (A) Negative control (no carcinoma cells). (B, D, G) Carcinoma cells (0.01, 0.001, and 0.0001%) were scanned in 3, 9, and 64 clusters, respectively, on the cell microarray chip. (C, E, F, H, I) Magnified views of the boxed regions. Color scale represents the intensity of fluorescent emission.

    Journal: PLoS ONE

    Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

    doi: 10.1371/journal.pone.0032370

    Figure Lengend Snippet: Detection of carcinoma cells among cultured T lymphoblastoid leukemia on a cell microarray chip. (A–I) Scanned images of leukocytes/carcinoma cells on a cell microarray chip obtained with the microarray scanner. (A) Negative control (no carcinoma cells). (B, D, G) Carcinoma cells (0.01, 0.001, and 0.0001%) were scanned in 3, 9, and 64 clusters, respectively, on the cell microarray chip. (C, E, F, H, I) Magnified views of the boxed regions. Color scale represents the intensity of fluorescent emission.

    Article Snippet: Each cell microarray chip was scanned for 20 min with a confocal laser-based fluorescence microarray scanner, CRBIO IIe (Hitachi Software Engineering Co., Ltd., Tokyo, Japan).

    Techniques: Cell Culture, Microarray, Chromatin Immunoprecipitation, Negative Control

    Detection of carcinoma cells among leukocytes/carcinoma cells isolated from whole blood and introduced onto a cell microarray chip. (A) Scanned images of cells on a cell microarray chip, obtained with the microarray scanner. The cells were immunostained with PE-labeled anti-cytokeratin monoclonal antibody. (B) Magnified view of the boxed region. Color scale represents the intensity of fluorescent emission.

    Journal: PLoS ONE

    Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

    doi: 10.1371/journal.pone.0032370

    Figure Lengend Snippet: Detection of carcinoma cells among leukocytes/carcinoma cells isolated from whole blood and introduced onto a cell microarray chip. (A) Scanned images of cells on a cell microarray chip, obtained with the microarray scanner. The cells were immunostained with PE-labeled anti-cytokeratin monoclonal antibody. (B) Magnified view of the boxed region. Color scale represents the intensity of fluorescent emission.

    Article Snippet: Each cell microarray chip was scanned for 20 min with a confocal laser-based fluorescence microarray scanner, CRBIO IIe (Hitachi Software Engineering Co., Ltd., Tokyo, Japan).

    Techniques: Isolation, Microarray, Chromatin Immunoprecipitation, Labeling

    Dispersion of carcinoma cells on a cell microarray chip and confinement in the microchambers. (A, B) Photographic light microscopic images of carcinoma cells on a cell microarray chip before (A) and after (B) washing of the chip surface. (C) Carcinoma cells showed tight confinement and had formed a monolayer in the microchamber when a concentration of 7.5×10 6 cells/ml was used. (Bar: 20 µm).

    Journal: PLoS ONE

    Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

    doi: 10.1371/journal.pone.0032370

    Figure Lengend Snippet: Dispersion of carcinoma cells on a cell microarray chip and confinement in the microchambers. (A, B) Photographic light microscopic images of carcinoma cells on a cell microarray chip before (A) and after (B) washing of the chip surface. (C) Carcinoma cells showed tight confinement and had formed a monolayer in the microchamber when a concentration of 7.5×10 6 cells/ml was used. (Bar: 20 µm).

    Article Snippet: Each cell microarray chip was scanned for 20 min with a confocal laser-based fluorescence microarray scanner, CRBIO IIe (Hitachi Software Engineering Co., Ltd., Tokyo, Japan).

    Techniques: Microarray, Chromatin Immunoprecipitation, Concentration Assay

    Dispersion of T lymphoblastoid leukemia cells on a cell microarray chip and confinement in the microchambers. (A, B) Photographic light microscopic images of T lymphoblastoid leukemia cells incubated on a cell microarray chip before (A) and after (B) washing of the chip surface. (C–F) Photos of microchamber appearance after washing when T lymphoblastoid leukemia suspensions of 2.5×10 6 (C), 5.0×10 6 (D), 7.5×10 6 (E) or 1.0×10 7 (F) cells/ml were applied to the microarray chip. Concentrations of 7.5×10 6 cells/ml of T lymphoblastoid leukemia and above afforded tight confinement and formation of a monolayer in the microchambers. (Bar: 20 µm).

    Journal: PLoS ONE

    Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

    doi: 10.1371/journal.pone.0032370

    Figure Lengend Snippet: Dispersion of T lymphoblastoid leukemia cells on a cell microarray chip and confinement in the microchambers. (A, B) Photographic light microscopic images of T lymphoblastoid leukemia cells incubated on a cell microarray chip before (A) and after (B) washing of the chip surface. (C–F) Photos of microchamber appearance after washing when T lymphoblastoid leukemia suspensions of 2.5×10 6 (C), 5.0×10 6 (D), 7.5×10 6 (E) or 1.0×10 7 (F) cells/ml were applied to the microarray chip. Concentrations of 7.5×10 6 cells/ml of T lymphoblastoid leukemia and above afforded tight confinement and formation of a monolayer in the microchambers. (Bar: 20 µm).

    Article Snippet: Each cell microarray chip was scanned for 20 min with a confocal laser-based fluorescence microarray scanner, CRBIO IIe (Hitachi Software Engineering Co., Ltd., Tokyo, Japan).

    Techniques: Microarray, Chromatin Immunoprecipitation, Incubation

    Dispersion of leukocytes isolated from whole blood on a cell microarray chip and confinement in the microchambers. (A, B) Photographic light microscopic images of leukocytes on a cell microarray chip before (A) and after (B) washing of the chip surface. (C) The leukocytes showed tight confinement and had formed a monolayer in the microchamber. (Bar: 20 µm).

    Journal: PLoS ONE

    Article Title: Accurate Detection of Carcinoma Cells by Use of a Cell Microarray Chip

    doi: 10.1371/journal.pone.0032370

    Figure Lengend Snippet: Dispersion of leukocytes isolated from whole blood on a cell microarray chip and confinement in the microchambers. (A, B) Photographic light microscopic images of leukocytes on a cell microarray chip before (A) and after (B) washing of the chip surface. (C) The leukocytes showed tight confinement and had formed a monolayer in the microchamber. (Bar: 20 µm).

    Article Snippet: Each cell microarray chip was scanned for 20 min with a confocal laser-based fluorescence microarray scanner, CRBIO IIe (Hitachi Software Engineering Co., Ltd., Tokyo, Japan).

    Techniques: Isolation, Microarray, Chromatin Immunoprecipitation

    Monitoring of antithrombotic therapy in mice using FLECT/CT. Thrombus was induced in the left carotid artery of mice by ferric chloride before the i.v. injection of activated-platelet targeting fluoroprobe, Targ-Cy7. A) Representative comparison of maximum-intensity projection FLECT/CT images of mice before and after treatment with either PBS or combined thrombolytic/anti-platelet drugs, urokinase/integrilin (n=3). The colour scale for each FLECT/CT image shows levels of detected NIR fluorescence, with white corresponding to the highest intensity and blue the lowest. B) The injured carotid artery was collected from each treated animal and scanned on the IVIS ® Lumina imager to confirm the detected FLECT NIR signal. A representative image of both control (PBS-treated) and urokinase/integrilin-treated left carotid vessels is shown. Detected Lumina fluorescence signal is depicted accordingly.

    Journal: Theranostics

    Article Title: A Unique Recombinant Fluoroprobe Targeting Activated Platelets Allows In Vivo Detection of Arterial Thrombosis and Pulmonary Embolism Using a Novel Three-Dimensional Fluorescence Emission Computed Tomography (FLECT) Technology

    doi: 10.7150/thno.18099

    Figure Lengend Snippet: Monitoring of antithrombotic therapy in mice using FLECT/CT. Thrombus was induced in the left carotid artery of mice by ferric chloride before the i.v. injection of activated-platelet targeting fluoroprobe, Targ-Cy7. A) Representative comparison of maximum-intensity projection FLECT/CT images of mice before and after treatment with either PBS or combined thrombolytic/anti-platelet drugs, urokinase/integrilin (n=3). The colour scale for each FLECT/CT image shows levels of detected NIR fluorescence, with white corresponding to the highest intensity and blue the lowest. B) The injured carotid artery was collected from each treated animal and scanned on the IVIS ® Lumina imager to confirm the detected FLECT NIR signal. A representative image of both control (PBS-treated) and urokinase/integrilin-treated left carotid vessels is shown. Detected Lumina fluorescence signal is depicted accordingly.

    Article Snippet: Similarly, in combination with a NIR scanner (Xiralite, Mivenion, GmbH), a non-specific ICG fluoroprobe has been used to detect joint hypervascularization in patients with rheumatoid arthritis .

    Techniques: Mouse Assay, Injection, Fluorescence

    Binding specificity of targeting-fluoroprobe (Targ-Cy7) to activated-platelets. A) Both targeting- (Targ-Cy7) and mutated- (Mut-Cy7) fluoroprobes were produced using the two-step conjugation system as evidenced by NIR fluorescence on the bands of interest (33 kDa), while controls Targ-BCN and Mut-BCN lacked any Cy7-800 nm fluorescence. B) The functionality of the Targ-Cy7 after the conjugation to bind activated-platelets was also ascertained. Ability to bind activated-platelet-rich thrombus (Figure 2 B top left panel: yellow indicating Cy7-800 nm fluorescence), in vitro activated-platelets (Figure 2 B bottom left panel: green indicating Cy7-800 nm fluorescence) and CHO cells expressing the activated GPIIb/IIIa (Figure 2 B right panel: green indicating Cy7-800 nm fluorescence) was only limited to the targeting FLECT fluoroprobe, Targ-Cy7.

    Journal: Theranostics

    Article Title: A Unique Recombinant Fluoroprobe Targeting Activated Platelets Allows In Vivo Detection of Arterial Thrombosis and Pulmonary Embolism Using a Novel Three-Dimensional Fluorescence Emission Computed Tomography (FLECT) Technology

    doi: 10.7150/thno.18099

    Figure Lengend Snippet: Binding specificity of targeting-fluoroprobe (Targ-Cy7) to activated-platelets. A) Both targeting- (Targ-Cy7) and mutated- (Mut-Cy7) fluoroprobes were produced using the two-step conjugation system as evidenced by NIR fluorescence on the bands of interest (33 kDa), while controls Targ-BCN and Mut-BCN lacked any Cy7-800 nm fluorescence. B) The functionality of the Targ-Cy7 after the conjugation to bind activated-platelets was also ascertained. Ability to bind activated-platelet-rich thrombus (Figure 2 B top left panel: yellow indicating Cy7-800 nm fluorescence), in vitro activated-platelets (Figure 2 B bottom left panel: green indicating Cy7-800 nm fluorescence) and CHO cells expressing the activated GPIIb/IIIa (Figure 2 B right panel: green indicating Cy7-800 nm fluorescence) was only limited to the targeting FLECT fluoroprobe, Targ-Cy7.

    Article Snippet: Similarly, in combination with a NIR scanner (Xiralite, Mivenion, GmbH), a non-specific ICG fluoroprobe has been used to detect joint hypervascularization in patients with rheumatoid arthritis .

    Techniques: Binding Assay, Produced, Conjugation Assay, Fluorescence, In Vitro, Expressing

    Circulatory in vivo half-life of the FLECT fluoroprobe and its use in FLECT/CT imaging of mice with left carotid ferric chloride induced thrombus. A) Mice (n=5) were i.v. injected with 1 µg/g of Targ-Cy7 and blood was collected at different time-points (0, 5, 30, 60 120, 240 and 1440 minutes). The NIR fluorescence signal in the collected samples was determined by the IVIS ® Lumina imager and quantified as shown. B) Upon arterial thrombus formation using the ferric chloride model, mice were i.v. injected with either mutated (Mut-Cy7; top panel) or targeting-fluoroprobe (Targ-Cy7; bottom panel) and allowed to circulate before they were scanned on the FLECT/CT imager. Following data reconstruction, coregistration and analysis, a representative comparison of maximum-intensity projection of FLECT/CT images of Mut-Cy7 (n=6) and Targ-Cy7 (n=6) mice is shown. The colour scale for each FLECT/CT image shows levels of detected NIR fluorescence with white corresponding to the highest intensity and blue the lowest. C) Using Invivoscope software, the region of interest around the left carotid artery was determined, and detected fluorescence intensity was quantified between groups of mice (**: p ≤ 0.01; Mann-Whitney nonparametric test, p= 0.0022). D) A representative micrograph of the ferric chloride-injured carotid artery (top) and the contralateral uninjured carotid artery (bottom), where nuclear stain (DAPI) is blue, and platelet-specific (CD41- Allophycocyanin) is red. E) Further analysis of the detected FLECT-signal in each mouse shows a strongly significant correlation to the weight of its ex-vivo thrombus (using Pearson's correlation analysis: r = 0.9807 and p= 0.0006, ***).

    Journal: Theranostics

    Article Title: A Unique Recombinant Fluoroprobe Targeting Activated Platelets Allows In Vivo Detection of Arterial Thrombosis and Pulmonary Embolism Using a Novel Three-Dimensional Fluorescence Emission Computed Tomography (FLECT) Technology

    doi: 10.7150/thno.18099

    Figure Lengend Snippet: Circulatory in vivo half-life of the FLECT fluoroprobe and its use in FLECT/CT imaging of mice with left carotid ferric chloride induced thrombus. A) Mice (n=5) were i.v. injected with 1 µg/g of Targ-Cy7 and blood was collected at different time-points (0, 5, 30, 60 120, 240 and 1440 minutes). The NIR fluorescence signal in the collected samples was determined by the IVIS ® Lumina imager and quantified as shown. B) Upon arterial thrombus formation using the ferric chloride model, mice were i.v. injected with either mutated (Mut-Cy7; top panel) or targeting-fluoroprobe (Targ-Cy7; bottom panel) and allowed to circulate before they were scanned on the FLECT/CT imager. Following data reconstruction, coregistration and analysis, a representative comparison of maximum-intensity projection of FLECT/CT images of Mut-Cy7 (n=6) and Targ-Cy7 (n=6) mice is shown. The colour scale for each FLECT/CT image shows levels of detected NIR fluorescence with white corresponding to the highest intensity and blue the lowest. C) Using Invivoscope software, the region of interest around the left carotid artery was determined, and detected fluorescence intensity was quantified between groups of mice (**: p ≤ 0.01; Mann-Whitney nonparametric test, p= 0.0022). D) A representative micrograph of the ferric chloride-injured carotid artery (top) and the contralateral uninjured carotid artery (bottom), where nuclear stain (DAPI) is blue, and platelet-specific (CD41- Allophycocyanin) is red. E) Further analysis of the detected FLECT-signal in each mouse shows a strongly significant correlation to the weight of its ex-vivo thrombus (using Pearson's correlation analysis: r = 0.9807 and p= 0.0006, ***).

    Article Snippet: Similarly, in combination with a NIR scanner (Xiralite, Mivenion, GmbH), a non-specific ICG fluoroprobe has been used to detect joint hypervascularization in patients with rheumatoid arthritis .

    Techniques: In Vivo, Imaging, Mouse Assay, Injection, Fluorescence, Software, MANN-WHITNEY, Staining, Ex Vivo

    IVIS ® Lumina scan of mice and their organs post FLECT/CT imaging. A) To verify the detected FLECT signal, each animal was subsequently imaged in the two-dimensional planar IVIS ® Lumina scanner. After euthanasia, both the right (uninjured) and left (injured) carotid arteries of each mouse were collected and similarly scanned in the IVIS ® Lumina scanner (Figure 4 A, bottom panel). B) In addition, major organs were also collected and scanned ex vivo . C) Quantitative analysis of the scanned organs with a region of interest (ROI) set at a fluorescence threshold of 25% was used to determine the levels of detected light radiance (un-normalized radiance to organ weight is shown). D) Analysis of the levels of detected ex vivo Targ-Cy7 signals of each injured left carotid vessel shows a significant correlation to its thrombus weight (using Pearson's correlation analysis (r = 0.8544 and p = 0.0303, *). E) Biodistribution of the NIR fluoroprobe uptake of these organs was also calculated by dividing the radiance with the mass (mg) of each scanned tissue for each group of mice (n=6) (Figure 4 E; Two-way ANOVA-Sidak's multiple group comparison test between Targ-Cy7 and Mut-Cy7 groups, Left Carotid- p= 0.0012. The p values between the 2 groups for rest of the organs (Right Carotid, Heart, Muscle, Brain, Intestine and Skin) are all > 0.9999).

    Journal: Theranostics

    Article Title: A Unique Recombinant Fluoroprobe Targeting Activated Platelets Allows In Vivo Detection of Arterial Thrombosis and Pulmonary Embolism Using a Novel Three-Dimensional Fluorescence Emission Computed Tomography (FLECT) Technology

    doi: 10.7150/thno.18099

    Figure Lengend Snippet: IVIS ® Lumina scan of mice and their organs post FLECT/CT imaging. A) To verify the detected FLECT signal, each animal was subsequently imaged in the two-dimensional planar IVIS ® Lumina scanner. After euthanasia, both the right (uninjured) and left (injured) carotid arteries of each mouse were collected and similarly scanned in the IVIS ® Lumina scanner (Figure 4 A, bottom panel). B) In addition, major organs were also collected and scanned ex vivo . C) Quantitative analysis of the scanned organs with a region of interest (ROI) set at a fluorescence threshold of 25% was used to determine the levels of detected light radiance (un-normalized radiance to organ weight is shown). D) Analysis of the levels of detected ex vivo Targ-Cy7 signals of each injured left carotid vessel shows a significant correlation to its thrombus weight (using Pearson's correlation analysis (r = 0.8544 and p = 0.0303, *). E) Biodistribution of the NIR fluoroprobe uptake of these organs was also calculated by dividing the radiance with the mass (mg) of each scanned tissue for each group of mice (n=6) (Figure 4 E; Two-way ANOVA-Sidak's multiple group comparison test between Targ-Cy7 and Mut-Cy7 groups, Left Carotid- p= 0.0012. The p values between the 2 groups for rest of the organs (Right Carotid, Heart, Muscle, Brain, Intestine and Skin) are all > 0.9999).

    Article Snippet: Similarly, in combination with a NIR scanner (Xiralite, Mivenion, GmbH), a non-specific ICG fluoroprobe has been used to detect joint hypervascularization in patients with rheumatoid arthritis .

    Techniques: Mouse Assay, Imaging, Ex Vivo, Fluorescence

    FLECT/CT imaging of mice with pulmonary embolism. Pulmonary embolism was induced in mice by the injection of thromborel. A) Following induction of embolism, mice were given (i.v.) either non-targeting (Mut-Cy7, n=3) or activated-platelet targeting-fluoroprobe (Targ-Cy7, n=4). Mice were then imaged in the FLECT/CT imager and generated images were reconstructed and analyzed as shown. To ensure the detected NIR signal in the Targ-Cy7 animal was localized to the lungs, mice were subsequently euthanized and airway perfused with CT contrasting medium, Iopromide, before an additional scan was performed (bottom panel). B) NIR fluorescence intensity from the region of interest was quantified in both groups (Mann-Whitney nonparametric test, p= 0.0286). C) Lungs were collected and histological analysis performed to detect CD41 positive (DAB stain; brown) thrombi within the pulmonary vessels. Each scale bar represents 20 µm of the micrograph.

    Journal: Theranostics

    Article Title: A Unique Recombinant Fluoroprobe Targeting Activated Platelets Allows In Vivo Detection of Arterial Thrombosis and Pulmonary Embolism Using a Novel Three-Dimensional Fluorescence Emission Computed Tomography (FLECT) Technology

    doi: 10.7150/thno.18099

    Figure Lengend Snippet: FLECT/CT imaging of mice with pulmonary embolism. Pulmonary embolism was induced in mice by the injection of thromborel. A) Following induction of embolism, mice were given (i.v.) either non-targeting (Mut-Cy7, n=3) or activated-platelet targeting-fluoroprobe (Targ-Cy7, n=4). Mice were then imaged in the FLECT/CT imager and generated images were reconstructed and analyzed as shown. To ensure the detected NIR signal in the Targ-Cy7 animal was localized to the lungs, mice were subsequently euthanized and airway perfused with CT contrasting medium, Iopromide, before an additional scan was performed (bottom panel). B) NIR fluorescence intensity from the region of interest was quantified in both groups (Mann-Whitney nonparametric test, p= 0.0286). C) Lungs were collected and histological analysis performed to detect CD41 positive (DAB stain; brown) thrombi within the pulmonary vessels. Each scale bar represents 20 µm of the micrograph.

    Article Snippet: Similarly, in combination with a NIR scanner (Xiralite, Mivenion, GmbH), a non-specific ICG fluoroprobe has been used to detect joint hypervascularization in patients with rheumatoid arthritis .

    Techniques: Imaging, Mouse Assay, Injection, Generated, Fluorescence, MANN-WHITNEY, Staining

    Two-step conjugation of scFv Targ for the production of FLECT fluoroprobe. A) Sortase bioconjugation of the targeting-scFv (scFv Targ ) to BCN resulted in the enzymatic product, BCN-Targ, of smaller protein size on the SDS PAGE gel (37 versus 33 kDa) due to cleavage of the His 6 -tag (Anti-His Western). B) The BCN-scFv was then conjugated to Cy7 dye via copper-free azide-cycloaddition 'click' reaction, generating the FLECT fluoroprobe (Targ-Cy7). Protein-gel analysis of the fluoroprobe was demonstrated to have NIR fluorescence (Odyssey Scan) on the band of interest (33 kDa), not seen on its unclicked-dye control (BCN-Targ) or addition of Cy7-dye to scFv Targ with or without exogenous BCN (non sortase-conjugated).

    Journal: Theranostics

    Article Title: A Unique Recombinant Fluoroprobe Targeting Activated Platelets Allows In Vivo Detection of Arterial Thrombosis and Pulmonary Embolism Using a Novel Three-Dimensional Fluorescence Emission Computed Tomography (FLECT) Technology

    doi: 10.7150/thno.18099

    Figure Lengend Snippet: Two-step conjugation of scFv Targ for the production of FLECT fluoroprobe. A) Sortase bioconjugation of the targeting-scFv (scFv Targ ) to BCN resulted in the enzymatic product, BCN-Targ, of smaller protein size on the SDS PAGE gel (37 versus 33 kDa) due to cleavage of the His 6 -tag (Anti-His Western). B) The BCN-scFv was then conjugated to Cy7 dye via copper-free azide-cycloaddition 'click' reaction, generating the FLECT fluoroprobe (Targ-Cy7). Protein-gel analysis of the fluoroprobe was demonstrated to have NIR fluorescence (Odyssey Scan) on the band of interest (33 kDa), not seen on its unclicked-dye control (BCN-Targ) or addition of Cy7-dye to scFv Targ with or without exogenous BCN (non sortase-conjugated).

    Article Snippet: Similarly, in combination with a NIR scanner (Xiralite, Mivenion, GmbH), a non-specific ICG fluoroprobe has been used to detect joint hypervascularization in patients with rheumatoid arthritis .

    Techniques: Conjugation Assay, SDS Page, Western Blot, Fluorescence

    Phantom image from the PEM/PET system. (a) Picture of the mini-Derenzo phantom (the numbers shown on the picture are the diameters of the rods in millimetres) (b) PEM/PET image of the phantom.

    Journal:

    Article Title: Initial clinical test of a breast-PET scanner

    doi: 10.1111/j.1754-9485.2010.02230.x

    Figure Lengend Snippet: Phantom image from the PEM/PET system. (a) Picture of the mini-Derenzo phantom (the numbers shown on the picture are the diameters of the rods in millimetres) (b) PEM/PET image of the phantom.

    Article Snippet: Specifically, our PEM/PET MLO images compare well in quality to those reported for the PEM scanner sold by Naviscan, Inc.

    Techniques: End-sequence Profiling, Positron Emission Tomography

    Picture of the PEM/PET system.

    Journal:

    Article Title: Initial clinical test of a breast-PET scanner

    doi: 10.1111/j.1754-9485.2010.02230.x

    Figure Lengend Snippet: Picture of the PEM/PET system.

    Article Snippet: Specifically, our PEM/PET MLO images compare well in quality to those reported for the PEM scanner sold by Naviscan, Inc.

    Techniques: End-sequence Profiling, Positron Emission Tomography

    Images from a patient with extremely dense breasts. (a) MLO view of the X-ray mammogram (b) MLO view of the FDG-PEM/PET scan of the breast showing the tumour and (c) transaxial PEM/PET view of the breast.

    Journal:

    Article Title: Initial clinical test of a breast-PET scanner

    doi: 10.1111/j.1754-9485.2010.02230.x

    Figure Lengend Snippet: Images from a patient with extremely dense breasts. (a) MLO view of the X-ray mammogram (b) MLO view of the FDG-PEM/PET scan of the breast showing the tumour and (c) transaxial PEM/PET view of the breast.

    Article Snippet: Specifically, our PEM/PET MLO images compare well in quality to those reported for the PEM scanner sold by Naviscan, Inc.

    Techniques: End-sequence Profiling, Positron Emission Tomography

    (a) Transaxial whole body PET/CT image showing the FDG-avid breast lesion and (b) MLO view of the PEM/PET image of the lesion, radiotracer uptake in an associated mammary duct is highlighted by the arrow.

    Journal:

    Article Title: Initial clinical test of a breast-PET scanner

    doi: 10.1111/j.1754-9485.2010.02230.x

    Figure Lengend Snippet: (a) Transaxial whole body PET/CT image showing the FDG-avid breast lesion and (b) MLO view of the PEM/PET image of the lesion, radiotracer uptake in an associated mammary duct is highlighted by the arrow.

    Article Snippet: Specifically, our PEM/PET MLO images compare well in quality to those reported for the PEM scanner sold by Naviscan, Inc.

    Techniques: Positron Emission Tomography, End-sequence Profiling

    (a) Transaxial view from a whole body PET/CT image showing the FDG-avid breast lesion and (b) MLO PEM/PET image (the arrow shows the likely FDG uptake in an occult lymph node).

    Journal:

    Article Title: Initial clinical test of a breast-PET scanner

    doi: 10.1111/j.1754-9485.2010.02230.x

    Figure Lengend Snippet: (a) Transaxial view from a whole body PET/CT image showing the FDG-avid breast lesion and (b) MLO PEM/PET image (the arrow shows the likely FDG uptake in an occult lymph node).

    Article Snippet: Specifically, our PEM/PET MLO images compare well in quality to those reported for the PEM scanner sold by Naviscan, Inc.

    Techniques: Positron Emission Tomography, End-sequence Profiling

    13-year-old male with comminuted right orbital roof “blow-in” fracture. Unenhanced CT orbits (obtained with a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare, using bone algorithm; axial acquisition of 0.6

    Journal:

    Article Title: Orbital Roof "Blow-in" Fracture: A Case Report and Review

    doi: 10.3941/jrcr.v3i12.363

    Figure Lengend Snippet: 13-year-old male with comminuted right orbital roof “blow-in” fracture. Unenhanced CT orbits (obtained with a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare, using bone algorithm; axial acquisition of 0.6

    Article Snippet: All imaging was obtained on a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare.

    Techniques:

    13-year-old male with comminuted right orbital roof “blow-in” fracture. Unenhanced CT orbits (obtained with a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare, using bone algorithm; axial acquisition of 0.6

    Journal:

    Article Title: Orbital Roof "Blow-in" Fracture: A Case Report and Review

    doi: 10.3941/jrcr.v3i12.363

    Figure Lengend Snippet: 13-year-old male with comminuted right orbital roof “blow-in” fracture. Unenhanced CT orbits (obtained with a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare, using bone algorithm; axial acquisition of 0.6

    Article Snippet: All imaging was obtained on a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare.

    Techniques:

    13-year-old male with comminuted right orbital roof “blow-in” fracture. Unenhanced CT orbits (obtained with a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare, using bone algorithm; axial acquisition of 0.6

    Journal:

    Article Title: Orbital Roof "Blow-in" Fracture: A Case Report and Review

    doi: 10.3941/jrcr.v3i12.363

    Figure Lengend Snippet: 13-year-old male with comminuted right orbital roof “blow-in” fracture. Unenhanced CT orbits (obtained with a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare, using bone algorithm; axial acquisition of 0.6

    Article Snippet: All imaging was obtained on a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare.

    Techniques:

    13-year-old male with comminuted right orbital roof “blow-in” fracture. Four day follow-up unenhanced CT orbits (obtained with a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare, using bone algorithm; axial

    Journal:

    Article Title: Orbital Roof "Blow-in" Fracture: A Case Report and Review

    doi: 10.3941/jrcr.v3i12.363

    Figure Lengend Snippet: 13-year-old male with comminuted right orbital roof “blow-in” fracture. Four day follow-up unenhanced CT orbits (obtained with a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare, using bone algorithm; axial

    Article Snippet: All imaging was obtained on a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare.

    Techniques:

    13-year-old male with comminuted right orbital roof “blow-in” fracture. Initial unenhanced CT head (obtained with a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare; axial acquisition of 0.6 mm thickness

    Journal:

    Article Title: Orbital Roof "Blow-in" Fracture: A Case Report and Review

    doi: 10.3941/jrcr.v3i12.363

    Figure Lengend Snippet: 13-year-old male with comminuted right orbital roof “blow-in” fracture. Initial unenhanced CT head (obtained with a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare; axial acquisition of 0.6 mm thickness

    Article Snippet: All imaging was obtained on a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare.

    Techniques:

    13-year-old male with comminuted right orbital roof “blow-in” fracture. Unenhanced CT orbits (obtained with a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare, using bone algorithm; axial acquisition of 0.6

    Journal:

    Article Title: Orbital Roof "Blow-in" Fracture: A Case Report and Review

    doi: 10.3941/jrcr.v3i12.363

    Figure Lengend Snippet: 13-year-old male with comminuted right orbital roof “blow-in” fracture. Unenhanced CT orbits (obtained with a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare, using bone algorithm; axial acquisition of 0.6

    Article Snippet: All imaging was obtained on a Siemens SOMATOM® Definition AS 128-slice CT scanner, Siemens Healthcare.

    Techniques: