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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: A Self-defeating Anabolic Program Leads to β-Cell Apoptosis in Endoplasmic Reticulum Stress-induced Diabetes via Regulation of Amino Acid Flux
doi: 10.1074/jbc.m113.466920
Figure Lengend Snippet: FIGURE 6. Attenuation of translational recovery during ER stress in Min6 cells promotes survival. A, [35S]Met/Cys incorporation into proteins in cells treated with Tg and Sal003 for the indicated times. Sal003 was added 6 h after initiation of Tg treatment as indicated by the scheme. B, Western blot analysis of the indicated proteins from cells treated with Tg and Sal003 (7.5 M for the last 6 or 12 h of Tg treatment). Antibodies against LRS and XPOT were from Abcam;othersarelistedinFig.1.C,LeuandMeAIBuptakeincellstreatedwith Tg and Sal003 (7.5 M) for the indicated times. D, cells were treated as indi- cated and apoptosis was assessed by measuring the percentage of sub-G1 cells by flow cytometry of propidium iodide-stained cells. Leu starvation was performed by replacing the media after 6 h of Tg treatment with Tg-contain- ing Leu-free medium. D-Leu (40 mM) or Sal003 (7.5 M) was added for the last 6 h of Tg treatment. Significant differences (p 0.01) from untreated cells (*) or the appropriate Tg-treated cells (**) are indicated. Error bars, S.E.
Article Snippet:
Techniques: Western Blot, Flow Cytometry, Staining