Journal: Journal of Neuroinflammation

Article Title: Microglial NCAM1 attenuates ischemic brain injury by inhibiting NF-κB-driven neuroinflammation through IκBα stabilization

doi: 10.1186/s12974-026-03766-7

Figure Lengend Snippet: NCAM1 overexpression in microglia/macrophages confers protection against neuroinflammation and apoptotic cell death after ischemic stroke in mouse tMCAO model. A Quantification for IL-1β, IL-6, TNF-α, CXCL1, and CCL2 mRNA expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice by qRT-PCR. n = 4. Results are represented as means ± SD. *** p < 0.001 vs. sham group and ## p < 0.01 and ### p < 0.001 vs. tMCAO plus Vector group. B Quantification for IL-1β, IL-6, TNF-α, CXCL1, and CCL2 protein expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice by ELISA. n = 4. Results are represented as means ± SD. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group. C and D Western blotting images and quantitative analysis for iNOS, CD32, and CD86 protein expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice. n = 4. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group. E and F Western blotting images and quantitative analysis for Bcl-xl, Bax, cleaved caspase-3, −9, and PARP protein expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice. n = 4. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group. G and H Representative immunofluorescence images and quantitative analysis for TUNEL-positive cells in the peri-infarct hippocampus and cortex of CX3CR1 Cre/ERT2 mice by TUNEL staining. n = 4. Scale bar: 50 μm. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group

Article Snippet: The levels of IL-1β, IL-6, TNF-α, CXCL1, and CCL2 in tissue homogenates were quantified using commercial mouse ELISA kits (Multi Sciences, Hangzhou, China), and the results were expressed as picograms per milliliter (pg/mL).

Techniques: Over Expression, Expressing, Quantitative RT-PCR, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, TUNEL Assay, Staining

Journal: Journal of Advanced Research

Article Title: Characterization of immune features and discovery of potential biomarkers for ankylosing spondylitis using deep plasma proteomics

doi: 10.1016/j.jare.2025.05.052

Figure Lengend Snippet: Verification of potential biomarkers for AS. (A) Expression levels of SAA1, FERMT3, ILK, and TLN1 in plasma samples from active-stage AS patients, stable-stage AS patients, and healthy controls measured by proteomics. ROC curves for the individual protein (SAA1, FERMT3, ILK, and TLN1) as a predictor to classify AS patients from healthy controls (B), and active-stage AS patients from stable-stage AS patients (C). (D) ELISA analysis of SAA1, FERMT3, ILK, and TLN1 expressions in the plasma samples from an independent validation cohort of healthy controls (N, n = 24), active-stage AS (A, n = 27), and stable-stage AS patients (S, n = 28). Statistical analyses employed Wilcoxon rank-sum test for intergroup comparisons, with results reported as mean ± SEM. Significance thresholds based on BH-adjusted p -values were defined as: *, <0.05; **, <0.01; ***, <0.001.

Article Snippet: The human serum amyloid A (SAA1) ELISA kit (#OKIA00083) was purchased from Aviva Systems Biology Company (San Diego, CA, USA).

Techniques: Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Biomarker Discovery

Journal: The European respiratory journal

Article Title: The NLRP3 inflammasome pathway is activated in sarcoidosis and involved in granuloma formation.

doi: 10.1183/13993003.00119-2019

Figure Lengend Snippet: Figure 4. TDM induced pulmonary granuloma in mice resemble human sarcoid granuloma: Involvment of the NLRP3 inflammasome pathway and reduced pulmonary granuloma burden in mice treated with compounds targeting the NLRP3 inflammasome. (A) H&E stain of lung harvested on day 7 from C57/BL6 wildtype (WT) mice challenged with TDM. (B) Counting strategy for granuloma burden of the left lobe of the lung. (C) High magnification of typical TDM-induced granulomas in mouse lung. (D) IL-1β release from BAL cells stimulated ex vivo with LPS/nigericin (6 h) was determined in the supernatants. (E ) H&E stained section of lung granulomas, red circles indicate the sites of higher magnification in F and G. what about green circles (F-H) Immunohistochemistry of the TDM induced granuloma using antibodies against the mouse macrophage lineage marker Iba-1 (F), IL-1β (G) or SAA what do red circles denote (H). (I-K) H&E stains of lung tissues from WT mice (I), miR-223-/- mice (J) and NLRP3-/- mice (K) 7d after TDM injection (each 6 replicates). (L) Granuloma counts of lung tissues from WT, miR-223 -/- mice and NLRP3-/- mice. Individual values and mean ± SD are shown. **p<0.01, unpaired t-test. (M) H&E stains of lungs from TDM-treated C57/BL6 wildtype mice treated with vehicle or the NLRP3 inflammasome pathway inhibitor MCC950 (each 2 replicates). (N) Reduced granuloma counts in lung tissues from MCC950- treated compared to vehicle-treated mice. Individual values and mean ± SD are shown. *p<0.05, unpaired ANOVA. (O) H&E stains of lung from TDM-treated C57/BL6 wildtype mice treated with isotype control antibody or anti-IL-1β antibody. (P) Significantly reduced granuloma counts in lung tissues from anti-IL-1β-treated compared to mice treated with isotype control antibody or vehicle (each 8 replicates). Individual values and mean ± standard deviation are shown. *p<0.05, unpaired ANOVA. Bar in panels A,B, 1000 μm; Bar in panel C 10 μm; bar in panel. E-G, and I-K 500 μm.

Article Snippet: Paraffin tissue sections from murine lung lobes (3μm) were stained with H&E and immunohistochemically using antibodies specific for mouse macrophage lineage marker Iba-1 (Wako Chemicals USA Inc., USA, 1:500), mouse IL-1β (Abcam, UK, 1:800), and SAA (Novus Biologicals, UK, 1:150).

Techniques: Staining, Ex Vivo, Immunohistochemistry, Marker, Injection, Control, Standard Deviation

Journal: OncoTargets and therapy

Article Title: Chemotherapy induces ovarian cancer cell repopulation through the caspase 3-mediated arachidonic acid metabolic pathway

doi: 10.2147/OTT.S150456

Figure Lengend Snippet: The PGE 2 and AA level and the expression of FAK in the clinical samples before and after chemotherapy. Notes: ( A ) The AA and PGE 2 levels of patient plasma samples were measured by ELISA. The PGE 2 -to-AA ratios of patient plasma samples before and after chemotherapy were compared. Three independent experiments were carried out. The results are expressed as the mean ± SD of three independent experiments, * P <0.05 compared with the control group. ( B ) Immunohistochemical staining of FAK and p-FAK in patient ovarian cancer samples before and after chemotherapy. ( C ) Levels of FAK and p-FAK were quantified according to immunohistochemical staining score. Abbreviations: PGE 2 , prostaglandin E 2 ; AA, arachidonic acid; FAK, focal adhesion kinase; ELISA, enzyme-linked immunosorbent assay; SD, standard deviation; p-FAK, phosphorylated FAK; IHC, immunohistochemical.

Article Snippet: A human PGE 2 enzyme-linked immunosorbent assay (ELISA) kit and human AA ELISA kit were purchased from R&D Systems.

Techniques: Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control, Immunohistochemical staining, Staining, Standard Deviation

Journal: OncoTargets and therapy

Article Title: Chemotherapy induces ovarian cancer cell repopulation through the caspase 3-mediated arachidonic acid metabolic pathway

doi: 10.2147/OTT.S150456

Figure Lengend Snippet: The AA and PGE 2 levels within the Transwell system. Notes: ( A and B ) The AA and PGE 2 levels of the supernatants collected each day from the Transwell system during the 10-day incubation were measured by ELISA. ( C ) The PGE 2 -to-AA ratios were calculated. Three independent experiments were carried out. The results are expressed as the mean ± SD, n=3, * P <0.05 compared with the control group. Abbreviations: AA, arachidonic acid; PGE 2 , prostaglandin E 2 ; ELISA, enzyme-linked immunosorbent assay; SD, standard deviation.

Article Snippet: A human PGE 2 enzyme-linked immunosorbent assay (ELISA) kit and human AA ELISA kit were purchased from R&D Systems.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Control, Standard Deviation