Journal: Scientific Reports

Article Title: Krüppel-like factor 6 is a transcriptional activator of autophagy in acute liver injury

doi: 10.1038/s41598-017-08680-w

Figure Lengend Snippet: Autophagosome formation in KLF6 over-expressing HepG2 cells. Under a transmission electron microscope, the autophagosome formation was observed and imaged in HepG2 cells transfected with the empty vector (pcIneo) ( A ), in HepG2 cells transfected with pcIneo treated with 15 µM rapamycin for 6 h to stimulate autophagosome formation ( B ) and in KLF6 -over-expressing HepG2 cells (pcIneo-KLF6) ( C ). Representative slides and blow-ups shown are shown of n = 2 independent cell culture experiments.

Article Snippet: For induction of autophagy and monitoring of autophagosome-formation, HepG2 cells were treated with 15 μM of Rapamycin for 6 h (Medchem Express, Monmouth Junctions, NJ USA).

Techniques: Expressing, Transmission Assay, Microscopy, Transfection, Plasmid Preparation, Cell Culture

Journal: Scientific Reports

Article Title: Krüppel-like factor 6 is a transcriptional activator of autophagy in acute liver injury

doi: 10.1038/s41598-017-08680-w

Figure Lengend Snippet: Autophagosome formation in KLF6 over-expressing HepG2 cells. To visualize formation of autophagosomes in HepG2 cells transfected with empty control vector (pcIneo) or in KLF6 over-expressing HepG2 cells (pcIneoKLF6) we treated the cells with Autophagy Tandem Sensor RFP-GFP-LC3B. As a positive control, we stimulated autophagosome formation via treatment with 15 µM rapamycin for 6 h. Cells were viewed and imaged with a Leica SP8 confocal microscope (20-fold magnification); shown are representative images of n = 3 individual cell culture experiments.

Article Snippet: For induction of autophagy and monitoring of autophagosome-formation, HepG2 cells were treated with 15 μM of Rapamycin for 6 h (Medchem Express, Monmouth Junctions, NJ USA).

Techniques: Expressing, Transfection, Control, Plasmid Preparation, Positive Control, Microscopy, Cell Culture