Journal: Cancer cell international

Article Title: Human drug efflux transporter ABCC5 confers acquired resistance to pemetrexed in breast cancer.

doi: 10.1186/s12935-021-01842-x

Figure Lengend Snippet: Fig. 1 Schematic diagram of animal study to evaluate the influence of ABCC5 expression on pemetrexed (MTA) efficacy

Article Snippet: ABCC5 human shRNA was purchased from OriGene (Locus ID: 10,057, Product ID: TL315024, Rockwell, USA).

Techniques: Expressing

Journal: Cancer cell international

Article Title: Human drug efflux transporter ABCC5 confers acquired resistance to pemetrexed in breast cancer.

doi: 10.1186/s12935-021-01842-x

Figure Lengend Snippet: Fig. 3 Assessment of transduction of ABCC5 adenovirus in MCF-7 cells. a ABCC5 expression was determined using western blotting after transduction with recombinant ABCC5-specific adenoviruses (AdvABCC5; multiplicity of infection (MOI) = 1000) or AdvCtrl (MOI = 1000) in MCF-7 cells for 48 h (n = 4), *p < 0.05 vs AdvCtrl. b ABCC5 expression was observed using immunofluorescence microscopy after transduction with recombinant AdvABCC5 or AdvCtrl. c ABCC5 mRNA expression determined using RT-PCR after transduction for 48 h. Data represent mean ± SD (n = 3), *p < 0.05

Article Snippet: ABCC5 human shRNA was purchased from OriGene (Locus ID: 10,057, Product ID: TL315024, Rockwell, USA).

Techniques: Transduction, Expressing, Western Blot, Recombinant, Infection, Immunofluorescence, Microscopy, Reverse Transcription Polymerase Chain Reaction

Journal: Cancer cell international

Article Title: Human drug efflux transporter ABCC5 confers acquired resistance to pemetrexed in breast cancer.

doi: 10.1186/s12935-021-01842-x

Figure Lengend Snippet: Fig. 5 ABCC5 affects the cell viability treated with MTA. a Dose– response curves of MCF-7, MCF-7-advABCC5, and MCF-7-siABCC5 cell lines. At least five drug concentrations were used to determine IC50 values. Experiments at each concentration were performed in triplicate. The presented data represent the means of three independent experiments. Data are shown as mean ± SD (n = 3, * p < 0.05). b The transductions of ABCC5 regulate cell apoptosis and expression of Bax and caspase after treatment with 0.1 μM MTA (42.74 ng/mL) for 72 h. The experiments were repeated in triplicate. An equal amount of cell lysate (20 μg of protein/lane) was analyzed using β-actin as the loading control (*p < 0.05, compared with non-MTA treatment group; #p < 0.05, compared with AdvCtrl group)

Article Snippet: ABCC5 human shRNA was purchased from OriGene (Locus ID: 10,057, Product ID: TL315024, Rockwell, USA).

Techniques: Concentration Assay, Expressing, Control

Journal: Cancer cell international

Article Title: Human drug efflux transporter ABCC5 confers acquired resistance to pemetrexed in breast cancer.

doi: 10.1186/s12935-021-01842-x

Figure Lengend Snippet: Fig. 6 Overexpression of ABCC5 in mice bearing subcutaneous BC xenografts reduces the antitumor activity of MTA. Tumor size was measured with a caliper (a). The excised tumors were weighed at the end point (b), and data are presented as mean ± SD (n = 6), *p < 0.05. Images of excised tumors from each group are shown (c)

Article Snippet: ABCC5 human shRNA was purchased from OriGene (Locus ID: 10,057, Product ID: TL315024, Rockwell, USA).

Techniques: Over Expression, Activity Assay

Journal: bioRxiv

Article Title: Ontogeny and Vulnerabilities of Drug-Tolerant Persisters in HER2+ Breast Cancer

doi: 10.1101/2020.08.28.273029

Figure Lengend Snippet: A, NPY1R expression in unsupervised clusters from untreated BT474 cells. B, UMAP showing supervised clustering of untreated BT474 single cells by their cell cycle gene expression with cells colored by NPY1R expression level. C, Plot showing NPY1R levels in untreated BT474 cells and gates used to define NPY1R hi , NPY1R mid , and NPY1R lo fractions. D, FACS-isolated cells from the indicated NPY1R fractions were treated with lapatinib for 14 days and then cultured in drug-free media for 14 days. Representative Incucyte images at the experimental endpoint are displayed, and a color mask is applied to show residual cells. E, NPY1R hi , NPY1R mid , and NPY1R lo BT474 cells were isolated by FACS and cultured under standard conditions. NPY1R expression was assessed by flow cytometry post-sort (Day 0) and at Days 4 and 14 of culture. F, FACS-isolated NPY1R cells were cultured under standard conditions, and viable cell number was quantified by Alamar Blue assay at the indicated times. Alamar Blue readings (RFU) were compared to the Day 0 values for each sample . G, Plot showing NPY1R levels in untreated BT474 cells and gates used to define NPY1R hi and NPY1R lo fractions (upper panel). ABCC5 expression is shown for NPY1R hi (lower right) and NPY1R lo (lower left) cells. H-J, ABCC5 hi and ABCC5 lo BT474 cells were isolated by FACS ( H ) and treated with lapatinib ( I ) or tucatinib ( J ) for 14 days. Cells were counted and compared to unsorted control cells. Mean ± SEM from three independent experiments is shown. Significance was assessed by one-way ANOVA with Tukey multiple comparisons test (**p<0.01, ***p<0.001, ****p<0.0001).

Article Snippet: Cells were released from plates by incubating with 0.05% trypsin and then stained with primary antibody (1 μg/10 6 live cells) against NPY1R (MAB6400; R&D Systems) followed by secondary antibody against human IgG for 30 minutes on ice and/or with PE-conjugated ABCC5 (sc-376965 PE; Santa Cruz).

Techniques: Expressing, Gene Expression, Isolation, Cell Culture, Flow Cytometry, Alamar Blue Assay, Control

Journal: Frontiers in Genetics

Article Title: Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma

doi: 10.3389/fgene.2022.805961

Figure Lengend Snippet: Combined effect survival analysis of ABCC1, ABCC4, ABCC5, and ABCC6.

Article Snippet: Antibodies for ABCC1, ABCC4, ABCC5, and ABCC6 were diluted according to the recommended concentrations of the manufacturer (Proteintech, Wuhan, China).

Techniques:

Journal: Frontiers in Genetics

Article Title: Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma

doi: 10.3389/fgene.2022.805961

Figure Lengend Snippet: ABCC1 , ABCC2 , ABCC4 , ABCC5 , ABCC6 , ABCC7 , ABCC9 , and ABCC10 were differently expressed between the HCC tissues and paraneoplastic tissues based on the RNA-seq data of the TCGA database: (A) ABCC1 , (B) ABCC2 , (C) ABCC4 , (D) ABCC5 , (E) ABCC6 , (F) ABCC7 , (G) ABCC9 , and (H) ABCC10 .

Article Snippet: Antibodies for ABCC1, ABCC4, ABCC5, and ABCC6 were diluted according to the recommended concentrations of the manufacturer (Proteintech, Wuhan, China).

Techniques: RNA Sequencing

Journal: Frontiers in Genetics

Article Title: Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma

doi: 10.3389/fgene.2022.805961

Figure Lengend Snippet: ROC curve for ABCC genes in the TCGA database: (A) ABCC5 , (B) ABCC7 , (C) ABCC9 , and (D) ABCC10 .

Article Snippet: Antibodies for ABCC1, ABCC4, ABCC5, and ABCC6 were diluted according to the recommended concentrations of the manufacturer (Proteintech, Wuhan, China).

Techniques:

Journal: Frontiers in Genetics

Article Title: Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma

doi: 10.3389/fgene.2022.805961

Figure Lengend Snippet: Survival analysis results of ABCC genes in the TCGA database.

Article Snippet: Antibodies for ABCC1, ABCC4, ABCC5, and ABCC6 were diluted according to the recommended concentrations of the manufacturer (Proteintech, Wuhan, China).

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma

doi: 10.3389/fgene.2022.805961

Figure Lengend Snippet: Survival curve of ABCC genes in the TCGA database: (A) ABCC1 , (B) ABCC4 , (C) ABCC5 , and (D) ABCC6.

Article Snippet: Antibodies for ABCC1, ABCC4, ABCC5, and ABCC6 were diluted according to the recommended concentrations of the manufacturer (Proteintech, Wuhan, China).

Techniques:

Journal: Frontiers in Genetics

Article Title: Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma

doi: 10.3389/fgene.2022.805961

Figure Lengend Snippet: Combined effect survival analysis of ABCC1 , ABCC4 , ABCC5 , and ABCC6 in the TCGA database: (A) combined effect survival analysis of ABCC1 and ABCC4 , (B) combined effect survival analysis of ABCC1 and ABCC5 , (C) combined effect survival analysis of ABCC1 and ABCC6 , (D) combined effect survival analysis of ABCC4 and ABCC5 , (E) combined effect survival analysis of ABCC4 and ABCC6 , and (F) combined effect survival analysis of ABCC5 and ABCC6 . The details about the groups are displayed in .

Article Snippet: Antibodies for ABCC1, ABCC4, ABCC5, and ABCC6 were diluted according to the recommended concentrations of the manufacturer (Proteintech, Wuhan, China).

Techniques:

Journal: Frontiers in Genetics

Article Title: Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma

doi: 10.3389/fgene.2022.805961

Figure Lengend Snippet: Nomogram and prognostic signature constructed in terms of ABCC1 , ABCC4 , ABCC5 , and ABCC6 in the TCGA database: (A) Nomogram based on expression of ABCC genes and clinicopathologic features; (B) internal validation for 1-year survival; (C) internal validation for 3-year survival; (D) internal validation for 5-year survival; (E) , scatter plot for risk score; (F) scatter plot for survival time (days); (G) , heat map corresponding to the expression of ABCC1 , ABCC4 , ABCC5 , and ABCC6 ; (H) survival analysis for high- and low-risk score groups; and (I) AUC for inspecting the efficiency of the prognostic signature for predicting long-term prognosis.

Article Snippet: Antibodies for ABCC1, ABCC4, ABCC5, and ABCC6 were diluted according to the recommended concentrations of the manufacturer (Proteintech, Wuhan, China).

Techniques: Construct, Expressing, Biomarker Discovery

Journal: Frontiers in Genetics

Article Title: Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma

doi: 10.3389/fgene.2022.805961

Figure Lengend Snippet: Validation for the prognostic significance of ABCC1 , ABCC4 , ABCC5 , and ABCC6 in the Guangxi HCC cohort: (A) expression of ABCC1, ABCC4, ABCC5, and ABCC6 in HCC tissues and paraneoplastic tissues assessed by IHC assays; (B–E) histogram showing ABCC1, ABCC4, ABCC5, and ABCC6 expression levels in HCC tissues and paraneoplastic tissues assessed by PCR assays; (F–I) survival curve of ABCC1, ABCC4, ABCC5, and ABCC6 in the Guangxi HCC cohort; the patients were grouped based on median expression; (J) Scatter plot for risk score; (K) scatter plot for survival time (months); (L) heat map corresponding to the expression of ABCC1 , ABCC4 , ABCC5 , and ABCC6 ; (M) survival analysis for high- and low-risk score groups; and (N) AUC for inspecting the efficiency of the prognostic signature for predicting long-term prognosis.

Article Snippet: Antibodies for ABCC1, ABCC4, ABCC5, and ABCC6 were diluted according to the recommended concentrations of the manufacturer (Proteintech, Wuhan, China).

Techniques: Biomarker Discovery, Expressing

Journal: Frontiers in Genetics

Article Title: Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma

doi: 10.3389/fgene.2022.805961

Figure Lengend Snippet: Survival analysis results of ABCC genes in the Guangxi cohort.

Article Snippet: Antibodies for ABCC1, ABCC4, ABCC5, and ABCC6 were diluted according to the recommended concentrations of the manufacturer (Proteintech, Wuhan, China).

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma

doi: 10.3389/fgene.2022.805961

Figure Lengend Snippet: GSEA results for the ABCC genes in the TCGA database: (A–F) GSEA results for ABCC1 in HCC, (G–L) GSEA results for ABCC4 in HCC, (M–O) GSEA results for ABCC5 in HCC, and (P–R) GSEA results for ABCC6 in HCC.

Article Snippet: Antibodies for ABCC1, ABCC4, ABCC5, and ABCC6 were diluted according to the recommended concentrations of the manufacturer (Proteintech, Wuhan, China).

Techniques:

Journal: Frontiers in Genetics

Article Title: Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma

doi: 10.3389/fgene.2022.805961

Figure Lengend Snippet: Correlation between ABCC gene expression and tumor-infiltrating immune cells: (A) scatter plot corresponding to ABCC1 expression and tumor-infiltrating immune cells, (B) scatter plot corresponding to ABCC4 expression and tumor-infiltrating immune cells, (C) scatter plot corresponding to ABCC5 expression and tumor-infiltrating immune cells, and (D) scatter plot corresponding to ABCC6 expression and tumor-infiltrating immune cells.

Article Snippet: Antibodies for ABCC1, ABCC4, ABCC5, and ABCC6 were diluted according to the recommended concentrations of the manufacturer (Proteintech, Wuhan, China).

Techniques: Gene Expression, Expressing