Journal: Biology Direct

Article Title: hsa_circ_0020093 suppresses ovarian cancer progression by modulating LRPPRC activity and miR-107/LATS2 signaling

doi: 10.1186/s13062-024-00520-y

Figure Lengend Snippet: hsa_circ_0020093 acts as miR-107 sponge to regulate LATS2 expression. A AGO2 RIP assay was executed in SKOV3 cells. B Venn diagram of the predicted target miRNAs of hsa_circ_0020093 identified from GSE47481, TargetScan, RegRNA and Starbase. C Differential expression of overlapped miRNAs across normal and high-grade serous ovarian cancer (HGSOC) samples in GSE47841. D Schematic illustration of hsa_circ_0020093-WT and hsa_circ_0020093-MUT luciferase reporter vectors. E-F The relative luciferase activities were detected in 293 T cells after co-transfection with hsa_circ_0020093-WT or hsa_circ_0020093-MUT and mimics or miRNC, respectively. G Venn diagram of the predicted target genes of miR-107 identified from RNA-seq data and three tools (TargetScan, miRTarbase, miRDB). H RT-qPCR detection of the expression of upregulated mRNAs in hsa_circ_0020093-overexpressed OC cells. I Western blot analysis of LATS2 protein level after hsa_circ_0020093 overexpression in OC cells. J Western blot analysis of LATS2 protein level after co-transfection of hsa_circ_0020093 or control and miR-107 or miRNC, respectively, in OC cells. P values were calculated by two-tailed Student’s t test. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: The miRNA microarray dataset GSE47841 was applied to analyze differentially expressed miRNAs between OC tissues and normal ovary tissues using dbDEMC database ( https://www.biosino.org/dbDEMC/index ).

Techniques: Expressing, Quantitative Proteomics, Luciferase, Cotransfection, RNA Sequencing, Quantitative RT-PCR, Western Blot, Over Expression, Control, Two Tailed Test