Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: Simultaneous determination of antiretroviral drugs in human hair with liquid chromatography-electrospray ionization-tandem mass spectrometry

doi: 10.1016/j.jchromb.2018.03.021

Figure Lengend Snippet: Ion pairs of analytes (a-b) tenofovir (TFV), (c-d) adefovir (ADV), (e-f) lamivudine (3TC), (g-h) lamivudine-d3 (3TC-d3), (i-j) nevirapine (NVP) and (k-l) nevirapine-d3 (NVP-d3). (a, c, e, g, i,k) precursor ion and (b, d, f, h, j, l) product ion.

Article Snippet: Standards, lamivudine, tenofovir and nevirapine were purchased from TargetMol (Shanghai, P.R.

Techniques:

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: Simultaneous determination of antiretroviral drugs in human hair with liquid chromatography-electrospray ionization-tandem mass spectrometry

doi: 10.1016/j.jchromb.2018.03.021

Figure Lengend Snippet: The chromatograms of analytes in blank hair matrix, blank hair matrix spiked with standard solutions and hair samples from HIV patients. (a-b) TFV, (c-d) 3TC, (e-f) NVP and (g-h) their ISs spiked with blank hair matrix. When spiked with 2-mg blank matrix (figures on the left), the standard concentrations were 500 pg/mg for TFV, 3TC and NVP. The typical concentrations of drugs detected in 2-mg hair samples were 435 pg/mg for TFV, 658 pg/mg for 3TC and 546 pg/mg for NVP. When spiked with 10-mg blank matrix (figures on the right), the standard concentrations were 1000 pg/mg for TFV and 100pg/mg for 3TC and NVP. The typical concentrations of drugs detected in 10-mg hair samples were 767 pg/mg for TFV, 97 pg/mg for 3TC and 131 pg/mg for NVP. The ISs’ concentrations were 200 ng/mL for adefovir and 20 ng/mL for 3TC-d3 and NVP-d3.

Article Snippet: Standards, lamivudine, tenofovir and nevirapine were purchased from TargetMol (Shanghai, P.R.

Techniques:

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: Simultaneous determination of antiretroviral drugs in human hair with liquid chromatography-electrospray ionization-tandem mass spectrometry

doi: 10.1016/j.jchromb.2018.03.021

Figure Lengend Snippet: Time courses of the ratio of the 3TC peak area to the peak area of 3TC-d3 in the incubation of 10-mg hair samples from one HIV patient who took 3TC.

Article Snippet: Standards, lamivudine, tenofovir and nevirapine were purchased from TargetMol (Shanghai, P.R.

Techniques: Incubation

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: Simultaneous determination of antiretroviral drugs in human hair with liquid chromatography-electrospray ionization-tandem mass spectrometry

doi: 10.1016/j.jchromb.2018.03.021

Figure Lengend Snippet: The determined sensitivity for (a-b) tenofovir, (c-d) lamivudine and (e-f) nevirapine spiked with and without 2-mg blank hair matrix (figures on the left) or 10-mg blank hair matrix (figures on the right). S is the sensitivity and R2 represents the square of correlation coefficient.

Article Snippet: Standards, lamivudine, tenofovir and nevirapine were purchased from TargetMol (Shanghai, P.R.

Techniques:

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: Simultaneous determination of antiretroviral drugs in human hair with liquid chromatography-electrospray ionization-tandem mass spectrometry

doi: 10.1016/j.jchromb.2018.03.021

Figure Lengend Snippet: Validation parameters of tenofovir, lamivudine and nevirapine for 2-mg hair.

Article Snippet: Standards, lamivudine, tenofovir and nevirapine were purchased from TargetMol (Shanghai, P.R.

Techniques:

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: Simultaneous determination of antiretroviral drugs in human hair with liquid chromatography-electrospray ionization-tandem mass spectrometry

doi: 10.1016/j.jchromb.2018.03.021

Figure Lengend Snippet: Validation parameters of tenofovir, lamivudine and nevirapine for 10-mg hair.

Article Snippet: Standards, lamivudine, tenofovir and nevirapine were purchased from TargetMol (Shanghai, P.R.

Techniques:

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: Simultaneous determination of antiretroviral drugs in human hair with liquid chromatography-electrospray ionization-tandem mass spectrometry

doi: 10.1016/j.jchromb.2018.03.021

Figure Lengend Snippet: Linear correlations in (a) 3TC and (b) NVP concentrations between the two methods based on 2-mg and 10-mg hair samples from HIV patients.

Article Snippet: Standards, lamivudine, tenofovir and nevirapine were purchased from TargetMol (Shanghai, P.R.

Techniques:

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: Simultaneous determination of antiretroviral drugs in human hair with liquid chromatography-electrospray ionization-tandem mass spectrometry

doi: 10.1016/j.jchromb.2018.03.021

Figure Lengend Snippet: The changes of 3TC concentration in 1-cm segment (Segment 1-6) and 2-cm segment (Segment 7-9) with the distance away from the scalp for HIV patients who used 3TC, (a) P01-P15, (b) P17-P27, P29-P32 and (c) mean. P16 and P28 did not take 3TC. P33 was excluded because her hair strands less than 10 mg in weight of Segment 2-9. Mean concentration was obtained from 26 patients because 4 patients (P21, P25, P26 and P30) with hair strands shorter than 12 cm in length and less than 10 mg in weight of Segment 7-9 were excluded. Hair segment 1 is closest to the scalp. Error bar in the figure is standard error mean.

Article Snippet: Standards, lamivudine, tenofovir and nevirapine were purchased from TargetMol (Shanghai, P.R.

Techniques: Concentration Assay

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: Simultaneous determination of antiretroviral drugs in human hair with liquid chromatography-electrospray ionization-tandem mass spectrometry

doi: 10.1016/j.jchromb.2018.03.021

Figure Lengend Snippet: The comparisons of concentrations in hair between the three drugs in the nine segments. (a) 3TC and TFV among 27 patients for Segment 1-6, among 26 patients for Segment 7-8 and among 23 patients for Segment 9 where HIV patients took 300 mg 3TC and TFV every day. (b) 3TC and NVP among 10 patients for Segment 1-5, among 9 patients for Segment 6, among 8 patients for Segment 7, among 7 patients for Segment 8 and among 6 patients for segment 9 where HIV patients took 300 mg 3TC and 400 mg NVP every day. The 3TC concentration was enlarged by 10 times and the NVP concentration in hair was normalized to 300 mg every day. (c) NVP and TFV among 8 patients for Segment 1-6 and among 7 patients for Segment 7 and among 6 patients for Segment 8 and among 5 patients for Segment 9 where HIV patients took 300 mg TFV and 400 mg NVP every day. The TFV concentration was enlarged by 10 times and the NVP concentration in hair was normalized to 300 mg every day. Error bar in the figure is standard error mean.

Article Snippet: Standards, lamivudine, tenofovir and nevirapine were purchased from TargetMol (Shanghai, P.R.

Techniques: Concentration Assay

Journal: Cells

Article Title: Polarized Cytokine Release Triggered by P2X7 Receptor from Retinal Pigmented Epithelial Cells Dependent on Calcium Influx

doi: 10.3390/cells9122537

Figure Lengend Snippet: Pharmaceutical validation of P2X7 receptor. Exposure of ARPE-19 cells to 100 µM BzATP in isotonic solution for 30 min triggered a release of IL-6 (*** vs. Control, p = 0.0005, n = 13). In the presence of BzATP, IL-6 release was reduced by P2X7 antagonists A438079 (50 µM, **** p < 0.0001, n = 3), AZ10606120 (50 µM AZ10606, * p = 0.0113, n = 6) and A839977 (50 µM, ** p = 0.0071, n = 9). No significant reduction (ns) in IL-6 levels was found when lamivudine (3TC, 100 µM, n = 12), MRS2176 (300 nM, n = 3) or NF 279 (50 nM, n = 3) were added in the presence of BzATP. One way ANOVA (F97,60) = 5.42, p < 0.0001; Dunnett’s multiple comparisons test vs. BzATP alone.

Article Snippet: All antagonists were purchased from Tocris/Biochine Corp. (Minneapolis, MN, USA) except for lamivudine (Toronto Research Chemicals, Toronto, ON, Canada).

Techniques: