Journal: Cell Communication and Signaling : CCS

Article Title: African swine fever virus A179L inhibits interferon induced transmembrane protein 1 activation of NF-κB pathway

doi: 10.1186/s12964-025-02381-y

Figure Lengend Snippet: ASFV A179L inhibits the NF-κB signaling pathway. A - B . HEK-293 A cells were co-transfected with pCAGGS-Flag-A179L and pRL-TK internal control plasmid, along with either pNF-κB-luc, pIFN-β-luc, or pISRE-luc reporter plasmid. The luciferase activity was detected at 24 24 h post-transfection. C - E . HEK-293 A cells were transfected with empty vector or pCAGGS-Flag-A179L for 24 h and then transfected with poly (I: C) for another 24 h. The mRNA levels of IL-1β and TNF-α were determined by RT-qPCR. F HEK-293 A cells were transfected with pCAGGS-Flag-A179L for 24 h, then treated with or without TNF-α (10 ng/mL) for 10 min, 20 min and 40 min. Protein levels of NF-κB, p-NF-κB, IκBα, and p-IκBα were analyzed by Western blot. G PAMs were transfected with shRNA-A179L (50 nM) or shRNA-NC (50 nM) for 8 h and then infected with ASFV. A179L mRNA levels were quantified using RT-qPCR at 6 and 12 hpi. H The transcription levels of the p72 (B646L) gene were detected using RT-qPCR. I Transcription levels of inflammatory cytokines were measured 12 h post-infection using RT-qPCR. J Protein levels of NF-κB, p-NF-κB, IκBα, and p-IκBα were analyzed by Western blot

Article Snippet: The following rabbit polyclonal antibodies were used: NF-κB (A3108), p-NF-κB (AP1355), p-IκBα (AP1069), IκBα (A1187), IKKβ (A0714), p-IKKβ (AP0546) and GFP (AE078) (Abclonal).

Techniques: Transfection, Control, Plasmid Preparation, Luciferase, Activity Assay, Quantitative RT-PCR, Western Blot, shRNA, Infection

Journal: Molecular cell

Article Title: LncRNA CamK-A Regulates Ca 2+ -Signaling-Mediated Tumor Microenvironment Remodeling.

doi: 10.1016/j.molcel.2018.08.014

Figure Lengend Snippet: Figure 5. Clinical Relevance of CamK-A in Ca2+-Dependent-NF-kB Axis in Breast Cancers (A) CamK-A levels were significantly associated with expression of p-IkBa (Ser32), Ki67, CD31, and F4/80 in 208 primary human breast cancer specimens (Sun Yat-sen cohorts). (Upper panel) Two representative cases are shown. The scale bars represent 100 mm. (Lower panel) Percentages of specimens showing low or high CamK-A expression relative to levels of p-IkBa (Ser32), Ki67, CD31, and F4/80 are shown. (B) Correlations between CamK-A levels and NF-kB target genes, including VEGF, IL-6, IL-8, TNFa, GLUT3, and MMP-9, in breast cancer tissues (n = 48). RNA levels were determined by qRT-PCR relative to B2M. The r values and p values are from Pearson’s correlation analysis. (C) qRT-PCR detection of PNCK expression in NBTs and malignant breast cancer; left panel, statistical analysis of training set (Sun Yat-sen cohorts; 48 NBT versus 48 malignant) and validation set (Sun Yat-sen cohorts; 48 NBT versus 48 malignant; Gehan-Breslow test).

Article Snippet: The full-length PNCK, IkBa expression vectors were obtained from Origene.

Techniques: Expressing, Quantitative RT-PCR, Biomarker Discovery