Journal: Stem cell reports

Article Title: DOT1L regulates lung developmental epithelial cell fate and adult alveolar stem cell differentiation after acute injury.

doi: 10.1016/j.stemcr.2023.07.006

Figure Lengend Snippet: Figure 1. Alveolar organoid screen to identify epigenetic pathways regulating alveolar regeneration (A) Schematic of how AT2 cells isolated from SftpcEGFP mice were used in the screen. (B) An example of fluorescent reporter detection from SftpcEGFP AT2 cells in a 96-well plate. Arrows indicate negative (water or DMSO) or positive (FGF7) controls used for the screen. (C) Results from the epigenetic small molecular inhibitor library using a Z score of 2.0 as a cutoff for a positive increase in alveolar organoid total fluorescence. Data are representative of the screening assay performed in duplicate.

Article Snippet: Forty-eight hours after seeding cells, medium was changed to include 10 mM of one small molecule from the epigenetic library (Selleckchem, L1900) or an equal volume of H2O or DMSO as negative controls and 50 ng/mL of Fgf7 as a positive control.

Techniques: Isolation, Screening Assay

Journal: Stem cell reports

Article Title: DOT1L regulates lung developmental epithelial cell fate and adult alveolar stem cell differentiation after acute injury.

doi: 10.1016/j.stemcr.2023.07.006

Figure Lengend Snippet: Figure 7. Model of how Dot1l regulation of AT2-AT1 differentiation through regula- tion of OxPhos and a subset of transcrip- tion and epigenetic factors

Article Snippet: Forty-eight hours after seeding cells, medium was changed to include 10 mM of one small molecule from the epigenetic library (Selleckchem, L1900) or an equal volume of H2O or DMSO as negative controls and 50 ng/mL of Fgf7 as a positive control.

Techniques:

Journal: Scientific Reports

Article Title: Histone acetyltransferase and Polo-like kinase 3 inhibitors prevent rat galactose-induced cataract

doi: 10.1038/s41598-019-56414-x

Figure Lengend Snippet: HAT inhibitor list.

Article Snippet: Epigenetic Multiple Ligand , p300/CBP , Santa Cruz (USA) , 50 μM , × , (29).

Techniques:

Journal: Annals of the Rheumatic Diseases

Article Title: Biological impact of iberdomide in patients with active systemic lupus erythematosus

doi: 10.1136/annrheumdis-2022-222212

Figure Lengend Snippet: Time course of change from baseline during iberdomide treatment in whole blood leucocyte counts and selected B cells, T cells and NK cells by flow cytometry (Covance, Indianapolis, Indiana, USA) (A), CD268, plasma blasts, switched memory B cells DC subset counts and plasma cells by flow cytometry (B) and Tregs, Tfh cells and Th17 cells by epigenetic assay (Epiontis ID, Epiontis GmbH, Berlin, Germany) (C). *p≤0.05; **p≤0.01; ***p≤0.001 vs placebo. Values shown are the treatment comparison vs placebo of adjusted mean per cent change from baseline. See for numeric data. BLyS, B lymphocyte stimulator; DC, dendritic cell; NK, natural killer; Tfh, T follicular helper; Th17, T helper 17; Tregs, regulatory T cells.

Article Snippet: T helper 17 (Th17) cells, regulatory T cells (Tregs) and T follicular helper (Tfh) cells were measured by epigenetic assays (Epiontis ID; Epiontis GmbH, Berlin, Germany), an approach that correlates strongly with flow cytometry.

Techniques: Flow Cytometry, Clinical Proteomics, Epigenetic Assay, Comparison

Journal: Nature Communications

Article Title: Mutation hotspots at CTCF binding sites coupled to chromosomal instability in gastrointestinal cancers

doi: 10.1038/s41467-018-03828-2

Figure Lengend Snippet: Summary of the data. a Gastric tumor samples grouped by cohort and ordered by SNV count within each cohort. The panels show coverage, SNV count, indel count, mutation spectrum, molecular subtype, and Lauren’s classification of each sample. b Correlations between epigenetic features and somatic mutation rates in different tumor subtypes (error bars represent s.e.m of the correlation coefficient). c Principle component analysis of contributions of epigenetic features to the variance in the mutation rate of individual tumors. Colored stacked bars show the contribution of individual epigenetic features to the first two principal components

Article Snippet: We investigated the correlations between somatic mutation rates of the four cancer subtypes and epigenetic profiles of gastric tissue obtained from the Roadmap Epigenomics project .

Techniques: Mutagenesis