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Image Search Results
Journal: Molecular Medicine Reports
Article Title: Polyphyllin VII induces apoptotic cell death via inhibition of the PI3K/Akt and NF-κB pathways in A549 human lung cancer cells
doi: 10.3892/mmr.2019.10879
Figure Lengend Snippet: Polyphyllin VII induces apoptosis of A549 cells via the PI3K/Akt and NF-κB pathways. A549 cells were treated with 0.41 µM Polyphyllin VII in the presence or absence of wortmannin and PDTC for 24 h. Protein expression levels were then evaluated by western blotting. GAPDH was used as a loading control for whole cell extract and lamin B was used as the loading control for nuclear extract. Densitometry results were expressed as average density relative to GAPDH or lamin B, or as a ratio of phosphorylated to total levels. Data are presented as mean ± standard deviation of 3 independent experiments. *P<0.05 and **P<0.01 vs. control group; # P<0.05 and ## P<0.01 vs. Polyphyllin VII-treated group. PDTC, pyrrolidine dithiocarbamate; p-, phosphorylated; PARP, poly-(ADP-ribose) polymerase; ICAD, inhibitor of caspase-activated DNase.
Article Snippet: Polyclonal antibodies against PI3K (cat. no. 20584-1-AP), Akt (cat. no. 10176-2-AP), NF-κB p65 (cat. no. 10745-1-AP), IκB (cat. no. 10268-1-AP), GAPDH (cat. no. 10494-1-AP), lamin B (cat. no. 12987-1-AP), caspase-3 (cat. no. 19677-1-AP), poly-(ADP-ribose) polymerase (PARP; cat. no. 13371-1-AP) and inhibitor of caspase-activated
Techniques: Expressing, Western Blot, Standard Deviation