Journal: Life

Article Title: Evaluation of an Antisense Oligonucleotide Targeting CAG Repeats: A Patient-Customized Therapy Study for Huntington’s Disease

doi: 10.3390/life14121607

Figure Lengend Snippet: Secondary structure analysis of normal Huntingtin mRNA exon 1 (NCBI NM_001388492.1) and mRNA with over 30 CAG repeats. ( A ) Secondary structure of wild-type exon 1 Huntingtin (NCBI NM_001388492.1). ( B ) Secondary structure with the incomplete hybridization predicted between wild-type exon 1 Huntingtin and ASO HTT 90-5, the red color highlights the antisense sequence. ( C ) mRNA secondary structure of the patients >30 CAG repeats sequence predicted by RNAfold with total hybridization. ( D ) Complete hybridization between the mRNA secondary structure of the patients >30 CAG repeats sequence and ASO HTT 90-5, the red color highlights the antisense sequence.

Article Snippet: Subsequently, for the Huntingtin mRNA expression, the RT-PCR was performed using primers designed for amplifying the mRNA Huntingtin gene; these primers were designed with the NCBI primer software Version 2.5.0 (National Center for Biotechnology Information, Bethesda, MD, USA, Available at: https://www.ncbi.nlm.nih.gov/tools/primer-blast/ , accessed on 11 April 2024), and the housekeeping gene was HPRT1, like the internal control.

Techniques: Hybridization, Sequencing

Journal: Life

Article Title: Evaluation of an Antisense Oligonucleotide Targeting CAG Repeats: A Patient-Customized Therapy Study for Huntington’s Disease

doi: 10.3390/life14121607

Figure Lengend Snippet: BLAST alignment of the antisense sequence ASO HTT 90-5 with the reference mRNA sequences Homo sapiens huntingtin transcript variant 1 (NCBI NM_001388492.1) and transcript variant 2 (NCBI NM_002111.8), as well as a sequence containing more than 30 CAG repeats, the asterisks indicate the nucleotides where homology between the sequences occurs.

Article Snippet: Subsequently, for the Huntingtin mRNA expression, the RT-PCR was performed using primers designed for amplifying the mRNA Huntingtin gene; these primers were designed with the NCBI primer software Version 2.5.0 (National Center for Biotechnology Information, Bethesda, MD, USA, Available at: https://www.ncbi.nlm.nih.gov/tools/primer-blast/ , accessed on 11 April 2024), and the housekeeping gene was HPRT1, like the internal control.

Techniques: Sequencing, Variant Assay