chip Search Results


zymo spin chip kit  (Zymo Research)
94
Zymo Research zymo spin chip kit
Zymo Spin Chip Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chip pcr  (Azenta)
86
Azenta chip pcr
Chip Pcr, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chip assays  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology chip assays
Figure 5 | Med23 promotes the transcription of negative regulators of T-cell activation. (a) Real-time reverse transcriptase-PCR assay for Egr1, Egr2, Klf2 and Foxp1 expression in freshly isolated or in vitro-stimulated naive CD4 þ Tcells. Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml <t>1)</t> <t>antibodies</t> (Egr1 and Egr2, n ¼ 7; Klf2 and Foxp1, n ¼ 6; * Po0.05, **Po0.01 and ***Po0.001 by Student’s t-test; NS, no significance). The relative transcription levels of target genes were normalized to Gapdh. Naı¨ve T cells were sorted by flow cytometry based on high expression of CD62L and low expression of CD44. (b) Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or in vitro stimulated and lysed for <t>ChIP</t> assays (Egr1 and Egr2, n ¼ 3; Klf2 and Foxp1, n ¼ 4; *Po0.05 by Student’s t-test; NS, no significance). (c) Ratio statistics for CD69 expression, as examined by flow cytometry, in stimulated naı¨ve CD4 þ Tcells transfected with lentiviral constructs. Purified naı¨ve CD4 þ Tcells transfected with vector, Egr2 or Klf2 lentiviral constructs were in vitro stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies and evaluated by fluorescence-activated cell sorting analysis (Vector and Egr2, n ¼ 8; Klf2, n ¼ 7; *Po0.05 and **Po0.01 by Student’s t-test). Error bars indicate s.e.m. All results are combined from at least three independent experiments.
Chip Assays, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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proteon glh sensor chip  (Bio-Rad)
93
Bio-Rad proteon glh sensor chip
Figure 5 | Med23 promotes the transcription of negative regulators of T-cell activation. (a) Real-time reverse transcriptase-PCR assay for Egr1, Egr2, Klf2 and Foxp1 expression in freshly isolated or in vitro-stimulated naive CD4 þ Tcells. Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml <t>1)</t> <t>antibodies</t> (Egr1 and Egr2, n ¼ 7; Klf2 and Foxp1, n ¼ 6; * Po0.05, **Po0.01 and ***Po0.001 by Student’s t-test; NS, no significance). The relative transcription levels of target genes were normalized to Gapdh. Naı¨ve T cells were sorted by flow cytometry based on high expression of CD62L and low expression of CD44. (b) Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or in vitro stimulated and lysed for <t>ChIP</t> assays (Egr1 and Egr2, n ¼ 3; Klf2 and Foxp1, n ¼ 4; *Po0.05 by Student’s t-test; NS, no significance). (c) Ratio statistics for CD69 expression, as examined by flow cytometry, in stimulated naı¨ve CD4 þ Tcells transfected with lentiviral constructs. Purified naı¨ve CD4 þ Tcells transfected with vector, Egr2 or Klf2 lentiviral constructs were in vitro stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies and evaluated by fluorescence-activated cell sorting analysis (Vector and Egr2, n ¼ 8; Klf2, n ¼ 7; *Po0.05 and **Po0.01 by Student’s t-test). Error bars indicate s.e.m. All results are combined from at least three independent experiments.
Proteon Glh Sensor Chip, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/proteon glh sensor chip/product/Bio-Rad
Average 93 stars, based on 1 article reviews
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chip it high sensitivity kit  (Active Motif)
96
Active Motif chip it high sensitivity kit
Figure 5 | Med23 promotes the transcription of negative regulators of T-cell activation. (a) Real-time reverse transcriptase-PCR assay for Egr1, Egr2, Klf2 and Foxp1 expression in freshly isolated or in vitro-stimulated naive CD4 þ Tcells. Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml <t>1)</t> <t>antibodies</t> (Egr1 and Egr2, n ¼ 7; Klf2 and Foxp1, n ¼ 6; * Po0.05, **Po0.01 and ***Po0.001 by Student’s t-test; NS, no significance). The relative transcription levels of target genes were normalized to Gapdh. Naı¨ve T cells were sorted by flow cytometry based on high expression of CD62L and low expression of CD44. (b) Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or in vitro stimulated and lysed for <t>ChIP</t> assays (Egr1 and Egr2, n ¼ 3; Klf2 and Foxp1, n ¼ 4; *Po0.05 by Student’s t-test; NS, no significance). (c) Ratio statistics for CD69 expression, as examined by flow cytometry, in stimulated naı¨ve CD4 þ Tcells transfected with lentiviral constructs. Purified naı¨ve CD4 þ Tcells transfected with vector, Egr2 or Klf2 lentiviral constructs were in vitro stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies and evaluated by fluorescence-activated cell sorting analysis (Vector and Egr2, n ¼ 8; Klf2, n ¼ 7; *Po0.05 and **Po0.01 by Student’s t-test). Error bars indicate s.e.m. All results are combined from at least three independent experiments.
Chip It High Sensitivity Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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lysis buffer  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology lysis buffer
Figure 5 | Med23 promotes the transcription of negative regulators of T-cell activation. (a) Real-time reverse transcriptase-PCR assay for Egr1, Egr2, Klf2 and Foxp1 expression in freshly isolated or in vitro-stimulated naive CD4 þ Tcells. Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml <t>1)</t> <t>antibodies</t> (Egr1 and Egr2, n ¼ 7; Klf2 and Foxp1, n ¼ 6; * Po0.05, **Po0.01 and ***Po0.001 by Student’s t-test; NS, no significance). The relative transcription levels of target genes were normalized to Gapdh. Naı¨ve T cells were sorted by flow cytometry based on high expression of CD62L and low expression of CD44. (b) Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or in vitro stimulated and lysed for <t>ChIP</t> assays (Egr1 and Egr2, n ¼ 3; Klf2 and Foxp1, n ¼ 4; *Po0.05 by Student’s t-test; NS, no significance). (c) Ratio statistics for CD69 expression, as examined by flow cytometry, in stimulated naı¨ve CD4 þ Tcells transfected with lentiviral constructs. Purified naı¨ve CD4 þ Tcells transfected with vector, Egr2 or Klf2 lentiviral constructs were in vitro stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies and evaluated by fluorescence-activated cell sorting analysis (Vector and Egr2, n ¼ 8; Klf2, n ¼ 7; *Po0.05 and **Po0.01 by Student’s t-test). Error bars indicate s.e.m. All results are combined from at least three independent experiments.
Lysis Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysis buffer/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
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rabbit anti histone γh2avd ps137  (Rockland Immunochemicals)
93
Rockland Immunochemicals rabbit anti histone γh2avd ps137
Figure 5 | Med23 promotes the transcription of negative regulators of T-cell activation. (a) Real-time reverse transcriptase-PCR assay for Egr1, Egr2, Klf2 and Foxp1 expression in freshly isolated or in vitro-stimulated naive CD4 þ Tcells. Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml <t>1)</t> <t>antibodies</t> (Egr1 and Egr2, n ¼ 7; Klf2 and Foxp1, n ¼ 6; * Po0.05, **Po0.01 and ***Po0.001 by Student’s t-test; NS, no significance). The relative transcription levels of target genes were normalized to Gapdh. Naı¨ve T cells were sorted by flow cytometry based on high expression of CD62L and low expression of CD44. (b) Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or in vitro stimulated and lysed for <t>ChIP</t> assays (Egr1 and Egr2, n ¼ 3; Klf2 and Foxp1, n ¼ 4; *Po0.05 by Student’s t-test; NS, no significance). (c) Ratio statistics for CD69 expression, as examined by flow cytometry, in stimulated naı¨ve CD4 þ Tcells transfected with lentiviral constructs. Purified naı¨ve CD4 þ Tcells transfected with vector, Egr2 or Klf2 lentiviral constructs were in vitro stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies and evaluated by fluorescence-activated cell sorting analysis (Vector and Egr2, n ¼ 8; Klf2, n ¼ 7; *Po0.05 and **Po0.01 by Student’s t-test). Error bars indicate s.e.m. All results are combined from at least three independent experiments.
Rabbit Anti Histone γh2avd Ps137, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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truseq chip seq sample preparation kit  (Illumina Inc)
96
Illumina Inc truseq chip seq sample preparation kit
Figure 5 | Med23 promotes the transcription of negative regulators of T-cell activation. (a) Real-time reverse transcriptase-PCR assay for Egr1, Egr2, Klf2 and Foxp1 expression in freshly isolated or in vitro-stimulated naive CD4 þ Tcells. Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml <t>1)</t> <t>antibodies</t> (Egr1 and Egr2, n ¼ 7; Klf2 and Foxp1, n ¼ 6; * Po0.05, **Po0.01 and ***Po0.001 by Student’s t-test; NS, no significance). The relative transcription levels of target genes were normalized to Gapdh. Naı¨ve T cells were sorted by flow cytometry based on high expression of CD62L and low expression of CD44. (b) Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or in vitro stimulated and lysed for <t>ChIP</t> assays (Egr1 and Egr2, n ¼ 3; Klf2 and Foxp1, n ¼ 4; *Po0.05 by Student’s t-test; NS, no significance). (c) Ratio statistics for CD69 expression, as examined by flow cytometry, in stimulated naı¨ve CD4 þ Tcells transfected with lentiviral constructs. Purified naı¨ve CD4 þ Tcells transfected with vector, Egr2 or Klf2 lentiviral constructs were in vitro stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies and evaluated by fluorescence-activated cell sorting analysis (Vector and Egr2, n ¼ 8; Klf2, n ¼ 7; *Po0.05 and **Po0.01 by Student’s t-test). Error bars indicate s.e.m. All results are combined from at least three independent experiments.
Truseq Chip Seq Sample Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chip dna clean concentrator kit  (Zymo Research)
99
Zymo Research chip dna clean concentrator kit
Figure 5 | Med23 promotes the transcription of negative regulators of T-cell activation. (a) Real-time reverse transcriptase-PCR assay for Egr1, Egr2, Klf2 and Foxp1 expression in freshly isolated or in vitro-stimulated naive CD4 þ Tcells. Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml <t>1)</t> <t>antibodies</t> (Egr1 and Egr2, n ¼ 7; Klf2 and Foxp1, n ¼ 6; * Po0.05, **Po0.01 and ***Po0.001 by Student’s t-test; NS, no significance). The relative transcription levels of target genes were normalized to Gapdh. Naı¨ve T cells were sorted by flow cytometry based on high expression of CD62L and low expression of CD44. (b) Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or in vitro stimulated and lysed for <t>ChIP</t> assays (Egr1 and Egr2, n ¼ 3; Klf2 and Foxp1, n ¼ 4; *Po0.05 by Student’s t-test; NS, no significance). (c) Ratio statistics for CD69 expression, as examined by flow cytometry, in stimulated naı¨ve CD4 þ Tcells transfected with lentiviral constructs. Purified naı¨ve CD4 þ Tcells transfected with vector, Egr2 or Klf2 lentiviral constructs were in vitro stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies and evaluated by fluorescence-activated cell sorting analysis (Vector and Egr2, n ¼ 8; Klf2, n ¼ 7; *Po0.05 and **Po0.01 by Student’s t-test). Error bars indicate s.e.m. All results are combined from at least three independent experiments.
Chip Dna Clean Concentrator Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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truseq sample preparation kit  (Illumina Inc)
96
Illumina Inc truseq sample preparation kit
Figure 5 | Med23 promotes the transcription of negative regulators of T-cell activation. (a) Real-time reverse transcriptase-PCR assay for Egr1, Egr2, Klf2 and Foxp1 expression in freshly isolated or in vitro-stimulated naive CD4 þ Tcells. Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml <t>1)</t> <t>antibodies</t> (Egr1 and Egr2, n ¼ 7; Klf2 and Foxp1, n ¼ 6; * Po0.05, **Po0.01 and ***Po0.001 by Student’s t-test; NS, no significance). The relative transcription levels of target genes were normalized to Gapdh. Naı¨ve T cells were sorted by flow cytometry based on high expression of CD62L and low expression of CD44. (b) Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or in vitro stimulated and lysed for <t>ChIP</t> assays (Egr1 and Egr2, n ¼ 3; Klf2 and Foxp1, n ¼ 4; *Po0.05 by Student’s t-test; NS, no significance). (c) Ratio statistics for CD69 expression, as examined by flow cytometry, in stimulated naı¨ve CD4 þ Tcells transfected with lentiviral constructs. Purified naı¨ve CD4 þ Tcells transfected with vector, Egr2 or Klf2 lentiviral constructs were in vitro stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies and evaluated by fluorescence-activated cell sorting analysis (Vector and Egr2, n ¼ 8; Klf2, n ¼ 7; *Po0.05 and **Po0.01 by Student’s t-test). Error bars indicate s.e.m. All results are combined from at least three independent experiments.
Truseq Sample Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microfluidic droplet generator  (Microfluidic ChipShop)
93
Microfluidic ChipShop microfluidic droplet generator
Figure 5 | Med23 promotes the transcription of negative regulators of T-cell activation. (a) Real-time reverse transcriptase-PCR assay for Egr1, Egr2, Klf2 and Foxp1 expression in freshly isolated or in vitro-stimulated naive CD4 þ Tcells. Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml <t>1)</t> <t>antibodies</t> (Egr1 and Egr2, n ¼ 7; Klf2 and Foxp1, n ¼ 6; * Po0.05, **Po0.01 and ***Po0.001 by Student’s t-test; NS, no significance). The relative transcription levels of target genes were normalized to Gapdh. Naı¨ve T cells were sorted by flow cytometry based on high expression of CD62L and low expression of CD44. (b) Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or in vitro stimulated and lysed for <t>ChIP</t> assays (Egr1 and Egr2, n ¼ 3; Klf2 and Foxp1, n ¼ 4; *Po0.05 by Student’s t-test; NS, no significance). (c) Ratio statistics for CD69 expression, as examined by flow cytometry, in stimulated naı¨ve CD4 þ Tcells transfected with lentiviral constructs. Purified naı¨ve CD4 þ Tcells transfected with vector, Egr2 or Klf2 lentiviral constructs were in vitro stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies and evaluated by fluorescence-activated cell sorting analysis (Vector and Egr2, n ¼ 8; Klf2, n ¼ 7; *Po0.05 and **Po0.01 by Student’s t-test). Error bars indicate s.e.m. All results are combined from at least three independent experiments.
Microfluidic Droplet Generator, supplied by Microfluidic ChipShop, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cm5 chip  (Danaher Inc)
96
Danaher Inc cm5 chip
Figure 5 | Med23 promotes the transcription of negative regulators of T-cell activation. (a) Real-time reverse transcriptase-PCR assay for Egr1, Egr2, Klf2 and Foxp1 expression in freshly isolated or in vitro-stimulated naive CD4 þ Tcells. Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml <t>1)</t> <t>antibodies</t> (Egr1 and Egr2, n ¼ 7; Klf2 and Foxp1, n ¼ 6; * Po0.05, **Po0.01 and ***Po0.001 by Student’s t-test; NS, no significance). The relative transcription levels of target genes were normalized to Gapdh. Naı¨ve T cells were sorted by flow cytometry based on high expression of CD62L and low expression of CD44. (b) Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or in vitro stimulated and lysed for <t>ChIP</t> assays (Egr1 and Egr2, n ¼ 3; Klf2 and Foxp1, n ¼ 4; *Po0.05 by Student’s t-test; NS, no significance). (c) Ratio statistics for CD69 expression, as examined by flow cytometry, in stimulated naı¨ve CD4 þ Tcells transfected with lentiviral constructs. Purified naı¨ve CD4 þ Tcells transfected with vector, Egr2 or Klf2 lentiviral constructs were in vitro stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies and evaluated by fluorescence-activated cell sorting analysis (Vector and Egr2, n ¼ 8; Klf2, n ¼ 7; *Po0.05 and **Po0.01 by Student’s t-test). Error bars indicate s.e.m. All results are combined from at least three independent experiments.
Cm5 Chip, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 5 | Med23 promotes the transcription of negative regulators of T-cell activation. (a) Real-time reverse transcriptase-PCR assay for Egr1, Egr2, Klf2 and Foxp1 expression in freshly isolated or in vitro-stimulated naive CD4 þ Tcells. Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies (Egr1 and Egr2, n ¼ 7; Klf2 and Foxp1, n ¼ 6; * Po0.05, **Po0.01 and ***Po0.001 by Student’s t-test; NS, no significance). The relative transcription levels of target genes were normalized to Gapdh. Naı¨ve T cells were sorted by flow cytometry based on high expression of CD62L and low expression of CD44. (b) Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or in vitro stimulated and lysed for ChIP assays (Egr1 and Egr2, n ¼ 3; Klf2 and Foxp1, n ¼ 4; *Po0.05 by Student’s t-test; NS, no significance). (c) Ratio statistics for CD69 expression, as examined by flow cytometry, in stimulated naı¨ve CD4 þ Tcells transfected with lentiviral constructs. Purified naı¨ve CD4 þ Tcells transfected with vector, Egr2 or Klf2 lentiviral constructs were in vitro stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies and evaluated by fluorescence-activated cell sorting analysis (Vector and Egr2, n ¼ 8; Klf2, n ¼ 7; *Po0.05 and **Po0.01 by Student’s t-test). Error bars indicate s.e.m. All results are combined from at least three independent experiments.

Journal: Nature communications

Article Title: The mediator subunit Med23 contributes to controlling T-cell activation and prevents autoimmunity.

doi: 10.1038/ncomms6225

Figure Lengend Snippet: Figure 5 | Med23 promotes the transcription of negative regulators of T-cell activation. (a) Real-time reverse transcriptase-PCR assay for Egr1, Egr2, Klf2 and Foxp1 expression in freshly isolated or in vitro-stimulated naive CD4 þ Tcells. Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies (Egr1 and Egr2, n ¼ 7; Klf2 and Foxp1, n ¼ 6; * Po0.05, **Po0.01 and ***Po0.001 by Student’s t-test; NS, no significance). The relative transcription levels of target genes were normalized to Gapdh. Naı¨ve T cells were sorted by flow cytometry based on high expression of CD62L and low expression of CD44. (b) Purified naı¨ve CD4 þ Tcells were unstimulated (fresh) or in vitro stimulated and lysed for ChIP assays (Egr1 and Egr2, n ¼ 3; Klf2 and Foxp1, n ¼ 4; *Po0.05 by Student’s t-test; NS, no significance). (c) Ratio statistics for CD69 expression, as examined by flow cytometry, in stimulated naı¨ve CD4 þ Tcells transfected with lentiviral constructs. Purified naı¨ve CD4 þ Tcells transfected with vector, Egr2 or Klf2 lentiviral constructs were in vitro stimulated using plate-bound anti-CD3 (1 mg ml 1) and anti-CD28 (1 mg ml 1) antibodies and evaluated by fluorescence-activated cell sorting analysis (Vector and Egr2, n ¼ 8; Klf2, n ¼ 7; *Po0.05 and **Po0.01 by Student’s t-test). Error bars indicate s.e.m. All results are combined from at least three independent experiments.

Article Snippet: The anti-Pol II (N-20) and normal rabbit IgG antibodies used in the ChIP assays (4mg per sample) were obtained from Santa Cruz Biotechnology.

Techniques: Activation Assay, Reverse Transcription, Expressing, Isolation, In Vitro, Cytometry, Transfection, Construct, Plasmid Preparation, FACS