Journal: Fungal Systematics and Evolution

Article Title: New and Interesting Fungi. 4

doi: 10.3114/fuse.2021.07.13

Figure Lengend Snippet: Collection details and GenBank accession numbers of isolates treated in this study, and associated ex-type strains where available. Species for which additional sequences were generated during the course of this study are also listed here.

Article Snippet: Closest hits using the tub2 sequence had highest similarity to Alternaria chartarum (strain ATCC 18044, GenBank JQ671994.1; Identities = 337/338 (99 %), no gaps), Alternaria septospora (strain CBS 109.38, GenBank JQ671995.1; Identities = 336/338 (99 %), no gaps), and Alternaria atra (strain ATCC 18040, GenBank JQ671998.1; Identities = 331/338 (98 %), no gaps).

Techniques: Generated, Capsules

Journal: Fungal Systematics and Evolution

Article Title: New and Interesting Fungi. 4

doi: 10.3114/fuse.2021.07.13

Figure Lengend Snippet: Alternaria chartarum ( CPC 38971). Conidiophores giving rise to chains of conidia. Scale bars = 10 μm.

Article Snippet: Closest hits using the tub2 sequence had highest similarity to Alternaria chartarum (strain ATCC 18044, GenBank JQ671994.1; Identities = 337/338 (99 %), no gaps), Alternaria septospora (strain CBS 109.38, GenBank JQ671995.1; Identities = 336/338 (99 %), no gaps), and Alternaria atra (strain ATCC 18040, GenBank JQ671998.1; Identities = 331/338 (98 %), no gaps).

Techniques:

Journal: Fungal Systematics and Evolution

Article Title: New and Interesting Fungi. 4

doi: 10.3114/fuse.2021.07.13

Figure Lengend Snippet: Alternaria heterospora (CPC 38969). Conidiophores giving rise to conidia. Scale bars = 10 μm.

Article Snippet: Closest hits using the tub2 sequence had highest similarity to Alternaria chartarum (strain ATCC 18044, GenBank JQ671994.1; Identities = 337/338 (99 %), no gaps), Alternaria septospora (strain CBS 109.38, GenBank JQ671995.1; Identities = 336/338 (99 %), no gaps), and Alternaria atra (strain ATCC 18040, GenBank JQ671998.1; Identities = 331/338 (98 %), no gaps).

Techniques:

Journal: Pharmaceuticals

Article Title: Dihydroartemisinin Promotes N1 Polarization of Tumor-Associated Neutrophils and Enhances Their Anti-Tumor Activity via Hub Gene Modulation

doi: 10.3390/ph19010088

Figure Lengend Snippet: ( A ) Flow cytometric analysis of CD11b expression following ATRA-induced differentiation. ( B ) qRT-PCR indicating CD11b mRNA levels after ATRA induction. ( C ) Wright’s staining of morphological changes in dNB4 cells (scale bar = 50 µm, 1000×). ( D ) qRT-PCR of hub genes including TNF, IL1B, PTGS2, BCL2A1, MSR1, ACOD1, CXCL16, CLEC10A and SOCS3 in DHA-treated dNB4 cells. ( E ) qRT-PCR of markers of N1 as PD-L1, NOX2, CEACAM8, and SIRL-1 in DHA-treated dNB4 cells. ( F ) ELISA of TNF, IL-1β, ROS, and PD-L1 in DHA-treated dNB4 cell supernatants. ( G ) CKA evaluation of the tumor-killing capacity of DHA-treated dNB4 cells in a direct co-culture system. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: All-trans retinoic acid (ATRA) was obtained from MCE (Monmouth Junction, NJ, UA, HY-14649).

Techniques: Expressing, Quantitative RT-PCR, Staining, Enzyme-linked Immunosorbent Assay, Co-Culture Assay

Journal: Redox Biology

Article Title: Targeting ALDH1A1 to enhance the efficacy of KRAS-targeted therapy through ferroptosis

doi: 10.1016/j.redox.2024.103361

Figure Lengend Snippet: ALDH1A1 inhibits ferroptosis through detoxifying aldehydes, promoting production of NADH and improving RARA function . (A – D) Measurement of retinal (A), 4-HNE (B), retinoic acid (C), and NADH (D) production in MIAPACA3 and H2122 cells with ALDH1A1 NC, inhibitor (CM10:500 nM), KO and OE under sotorasib or adagrasib treatment (IC50,IC90, Peak serum concentration, 72 h) (n = 3). (E) Relative viability showed retinoic acid receptor α (RARA) resisted KRAS G12C mutant cells to ferroptosis caused by RSL3, IKE, sotorasib and adagtasib (IC50), demonstrated by medications with RARA inhibitors AGN193109 (1000 nM) or activator ATRA (1000 nM) for 72 h. (n = 3) (F) Lipid peroxidation of KRAS G12C mutant cells increased when added AGN193109 (1000 nM) to sotorasib or adagrasib (IC50) for 72 h. (n = 3) (G) Dual-luciferase assays reflected significant RARA activity depression in ALDH1A1 -KO and CM10 (500 nM) treated groups (MIAPACA2 and H2122 cells) under KRAS G12C inhibitors medications (IC50). (n = 3) Data were analyzed by Student's t-test and were presented by mean ± SD.

Article Snippet: Various doses of KRAS-targeted inhibitors, RSL3, IKE, fer-1, Z -VAD, DFO, necro, CM10, ATRA, AGN193109, were used to treat the cells for 72 h. The Cell Counting Kit-8 (TargetMol) was used to gauge the activity of cellular dehydrogenases as an indicator of cell viability.

Techniques: Concentration Assay, Mutagenesis, Medications, Luciferase, Activity Assay