Journal: Scientific Reports

Article Title: Endothelial Cell Phenotypes are Maintained During Angiogenesis in Cultured Microvascular Networks

doi: 10.1038/s41598-018-24081-z

Figure Lengend Snippet: Illustration of CD36 labeling in ex vivo cultured microvascular networks and stimulated in vivo microvasculature. Comparison between Day 3 ( Ex Vivo ) ( A–C ) and Day 3 ( In Vivo ) ( D–F ) microvascular networks revealed diminishing CD36 labeling along the length of the capillary sprout becoming absent at the tip. Arrows identify examples of CD36-negative capillary sprouts. Scale bars = 200 µm. ( G , H ) Capillary sprouts exhibited a substantial decrease in CD36 labeling compared to larger network vessels ( > 10 µm) in Day 3 ( Ex Vivo ) and Day 3 ( In Vivo ) tissues. White and black bars represent “Sprouts” and “Networks” respectively for Day 3 ( Ex Vivo ) and Day 3 ( In Vivo ) groups. The *** indicates a significant difference of p < 0.001 by Mann-Whitney U test.

Article Snippet: The following primary antibodies were used: UNC5b (1:100; Abcam; Cambridge, MA), VEGFR-2 (1:50; Santa Cruz Biotechnology; Dallas, TX), Alexa-568 Phalloidin (1:50; Invitrogen; Carlsbad, CA), CD36 (1:100; Novus Biologicals; Littleton, CO), and PECAM (1:200; BD Pharmingen; San Jose, CA).

Techniques: Labeling, Ex Vivo, Cell Culture, In Vivo, Comparison, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Reduction of the HIV Protease Inhibitor-Induced ER Stress and Inflammatory Response by Raltegravir in Macrophages

doi: 10.1371/journal.pone.0090856

Figure Lengend Snippet: Real time PCR primers.

Article Snippet: Mouse monoclonal antibody against CD36 was from Cayman Chemical (Ann Arbor, MI).

Techniques: Real-time Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: Reduction of the HIV Protease Inhibitor-Induced ER Stress and Inflammatory Response by Raltegravir in Macrophages

doi: 10.1371/journal.pone.0090856

Figure Lengend Snippet: ( A–C ) Human THP-1-derived macrophages were treated with HIV PIs (lopinavir/Ritonavir, LOPV/RITV, 25 µM) with or without raltegravir (25 µM) for 24 h. The total cellular RNA was isolated and reverse transcribed. The relative mRNA levels of CD36, SRA, and LDLR were determined by real-time PCR as described in “Methods”. The values are means ± S.E. of three independent experiments. **, p<0.01, ***, p<0.001, statistical significance relative to vehicle control. #, p<0.05, statistical significance of HIV PI+raltegravir-treated group relative to HIV PI-treated group. ( D ) Human THP-1-derived macrophages were treated with HIV PIs (lopinavir/Ritonavir, LOPV/RITV, 25 µM) with or without raltegravir (25 µM) for 24 h. The total protein lysates were prepared and subjected to Western blot analysis. Representative immunoblots for CD36, SRA and β-actin are shown. β-actin was used as the loading control for total proteins.

Article Snippet: Mouse monoclonal antibody against CD36 was from Cayman Chemical (Ann Arbor, MI).

Techniques: Derivative Assay, Isolation, Reverse Transcription, Real-time Polymerase Chain Reaction, Control, Western Blot

Journal: Cell Death & Disease

Article Title: Microparticles from apoptotic platelets promote resident macrophage differentiation

doi: 10.1038/cddis.2011.94

Figure Lengend Snippet: Differentiation of monocytes into resident phagocytic cells induced by PM ap . ( a ) Surface spreading and actin cytoskeleton rearrangements of THP-1 cells, cultured for 7 days with vehicle buffer or PM ap . Bright-field images indicate cell spreading and filopod formation (arrows); images of FITC-phalloidin fluorescence show strands of actin filaments with PM ap (scale bars: 100 μ m, representative for three experiments). ( b ) Proliferation of THP-1 cells after 7 days of incubation with vehicle or PM ap . Data are expressed as absolute cell numbers. ( c ) Relative amount (%) of necrotic cells after incubation with PMA (10 ng/ml), PM ap , or resting platelets. ( d ) Uptake of oxLDL (labeled with DiI) by THP-1 cells, incubated for 7 days with PM ap or resting platelets (plts). ( e and f ,) Expression of phagocytic cell markers on THP-1 cells ( e ) and primary monocytes ( f ). Cells were treated for 2 or 7 days with vehicle or PM ap . Surface expression of CD36 was determined by flow cytometry, as was the intracellular expression of CD68 in permeabilized cells. Incubation conditions were as described for . Mean±S.E.M. ( n =4–5). * P <0.05 versus control

Article Snippet: THP-1 cells or monocytes in suspension were stained (30 min, 4 °C) with mouse anti-human antibodies against CD11b, CD31 (Sigma, St. Louis, MO, USA); CD14, CD16, CCR2, CCR5, CXCR4 (BD Biosciences); or CD36 (ImmunoTools, Friesoythe, Germany) and prepared as described before.

Techniques: Cell Culture, Fluorescence, Incubation, Labeling, Expressing, Flow Cytometry

Journal: Frontiers in Immunology

Article Title: Unveiling macrophage dynamics and efferocytosis-related targets in diabetic kidney disease: insights from single-cell and bulk RNA-sequencing

doi: 10.3389/fimmu.2025.1521554

Figure Lengend Snippet: Screening hub genes related to efferocytosis in diabetic kidney macrophages. The GO enrichment analysis of macrophage subpopulations in 3-month-old (A) and 7-month-old mice (B) . (C) The chord diagram about the interaction among renal immune cells. (D) The Venn diagram illustrating the intersection between GSE195799 and the gene list of efferocytosis. (E) The PPI diagram, where the color varies from light to dark, signifies an ascending order of degree. (F–H) Violin diagrams of the distribution of CD36, ITGAM, and CX3CR1 in each cluster. GO, gene ontology; MIF, macrophage migration inhibitory factor; PPI, protein-protein interaction network.

Article Snippet: Afterwards, the primary antibody incubation was performed at 4°C overnight, encompassing antibodies for CD36 (A19016, ABclonal, China), ITGAM (ab133357, Abcam, Cambridge, UK), and CX3CR1 (13885-1-AP, Proteintech, China).

Techniques: Migration

Journal: Frontiers in Immunology

Article Title: Unveiling macrophage dynamics and efferocytosis-related targets in diabetic kidney disease: insights from single-cell and bulk RNA-sequencing

doi: 10.3389/fimmu.2025.1521554

Figure Lengend Snippet: Validating the clinical relevance of hub molecules. (A–C) illustrating the distribution characteristics of the data via violin plots. Scatter plots describe the correlation between CD36 (D) , ITGAM (E) , and CX3CR1 (F) expression with clinical indicators. ***P<0.001.

Article Snippet: Afterwards, the primary antibody incubation was performed at 4°C overnight, encompassing antibodies for CD36 (A19016, ABclonal, China), ITGAM (ab133357, Abcam, Cambridge, UK), and CX3CR1 (13885-1-AP, Proteintech, China).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Unveiling macrophage dynamics and efferocytosis-related targets in diabetic kidney disease: insights from single-cell and bulk RNA-sequencing

doi: 10.3389/fimmu.2025.1521554

Figure Lengend Snippet: Evaluation of target gene expression in DKD model. (A) Quantitative PCR analysis of ITGAM, CD36, and CX3CR1 mRNA expression. (B) Western blot showing the protein expression levels of ITGAM, CD36, and CX3CR1. (C) Quantitative analysis of protein band grayscale values for ITGAM, CD36, and CX3CR1. *P < 0.05, **P < 0.01, ***P<0.001.

Article Snippet: Afterwards, the primary antibody incubation was performed at 4°C overnight, encompassing antibodies for CD36 (A19016, ABclonal, China), ITGAM (ab133357, Abcam, Cambridge, UK), and CX3CR1 (13885-1-AP, Proteintech, China).

Techniques: Targeted Gene Expression, Real-time Polymerase Chain Reaction, Expressing, Western Blot