Journal: Oncology Letters

Article Title: Notch pathway inhibitor DAPT accelerates in vitro proliferation and adipogenesis in infantile hemangioma stem cells

doi: 10.3892/ol.2021.13115

Figure Lengend Snippet: Primer sequences used for reverse transcription-quantitative PCR.

Article Snippet: Additional cultures were prepared and protein samples were collected to evaluate the effects of DAPT treatment on the expression levels of β-actin (dilution, 1:500; cat. no. 3700; Cell Signaling Technology, Inc.), PPARγ (dilution, 1:500; cat. no. BS-0530R-2; BIOSS), C/EBPα (dilution, 1:1,000; cat. no. BS-1630R; BIOSS), C/EBPβ (dilution, 1:1,000; cat. no. BS-1396R; BIOSS), adiponectin (dilution, 1:1,000; cat. no. BS-0471R; BIOSS) and IGF-1 (dilution, 1:1,000; cat. no. BS-0014R; BIOSS).

Techniques: Sequencing

Journal: Oncology Letters

Article Title: Notch pathway inhibitor DAPT accelerates in vitro proliferation and adipogenesis in infantile hemangioma stem cells

doi: 10.3892/ol.2021.13115

Figure Lengend Snippet: Exposure to DAPT promotes adipogenesis and hemangioma stem cell expression of adiponectin, C/EBPα, C/EBPβ and PPARγ. Expression levels of (A) adiponectin, (B) C/EBPα, (C) C/EBPβ, (D) PPARγ and (E) IGF-1. In addition, expression was evaluated using (F) western blotting and semi-quantified using β-actin as the loading control. The band densities of (G) adiponectin, (H) C/EBPα, (I) C/EBPβ, (J) PPARγ and (K) IGF-1 were analyzed using ImageJ software. n=3. ns, P>0.05, *P<0.05, **P<0.01 (n=3). ADIP, adipogenic medium; ADIP+DAPT, adipogenic medium containing DAPT; ADIP+DMSO, adipogenic medium containing DMSO; C/EBPα, CCAAT/enhancer-binding protein α; C/EBPβ, CCAAT/enhancer-binding protein β; CON, control; DAPT, N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester; IGF-1, insulin-like growth factor 1; ns, not significant; PPARγ, peroxisome proliferator-activated receptor-γ.

Article Snippet: Additional cultures were prepared and protein samples were collected to evaluate the effects of DAPT treatment on the expression levels of β-actin (dilution, 1:500; cat. no. 3700; Cell Signaling Technology, Inc.), PPARγ (dilution, 1:500; cat. no. BS-0530R-2; BIOSS), C/EBPα (dilution, 1:1,000; cat. no. BS-1630R; BIOSS), C/EBPβ (dilution, 1:1,000; cat. no. BS-1396R; BIOSS), adiponectin (dilution, 1:1,000; cat. no. BS-0471R; BIOSS) and IGF-1 (dilution, 1:1,000; cat. no. BS-0014R; BIOSS).

Techniques: Expressing, Western Blot, Software, Binding Assay

Journal: Cancer Medicine

Article Title: Exploring the Relationship Between Adipocytokines and Endometrial Cancer: Identifying Correlations With Clinico‐Pathological Prognostic Factors

doi: 10.1002/cam4.71007

Figure Lengend Snippet: (A, B) Bar‐graph demonstrating the expression of (A) biomarkers and (B) their receptors in endometrial cancer tissue ( n = 39). X axis‐ biomarkers (A), receptors (B). Y axis–expressions of biomarkers as fold change over benign endometrial calibrator sample (assigned value = 1, for comparison with the cancer samples). The error bars represent standard errors of mean. 1 (C) Heat map demonstrating the expression of all 6 biomarkers and their receptors in endometrial cancer tissues ( n = 39). AdipoQ, adiponectin; ADIPOR1/R2, adiponectin receptors; OBR, leptin receptor; TNF1A, TNFRSF1A; TNF1B, TNFRSF1B (TNF receptors). Gene expressions are plotted on the Y ‐axis against the individual patients on the X ‐axis. Gene expressions are plotted as fold changes over a single pooled calibrator sample value. The color scale ranges from black (lowest value) to white (median) to yellow (highest value).

Article Snippet: The antibodies used were: ADIPOR1 (Proteintech, Manchester, UK, #66619‐1‐Ig; anti‐mouse antibody) and ADIPOR2 (Abcam, Cambridge, UK, #ab223752; anti‐rabbit antibody).

Techniques: Expressing, Comparison

Journal: Cancer Medicine

Article Title: Exploring the Relationship Between Adipocytokines and Endometrial Cancer: Identifying Correlations With Clinico‐Pathological Prognostic Factors

doi: 10.1002/cam4.71007

Figure Lengend Snippet: Heat map comparing the expression of (A) adiponectin, leptin and their receptors in endometrial cancer tissue and parametrial adipose tissue ( n = 39) (B) all 6 biomarkers and their receptors in endometrial cancer tissue and lymph nodes ( n = 12). AdipoQ, adiponectin; AdipoR1/R2, adiponectin receptors; ObR, leptin receptor; TNF1A, TNFRSF1A; TNF1B, TNFRSF1B (TNF receptors). Gene expressions are plotted on the Y ‐axis against the individual patients on the X ‐axis. Gene expression plotted as fold changes over a single pooled calibrator sample. The color scale ranges from black (lowest value) to white (median) to yellow (highest value).

Article Snippet: The antibodies used were: ADIPOR1 (Proteintech, Manchester, UK, #66619‐1‐Ig; anti‐mouse antibody) and ADIPOR2 (Abcam, Cambridge, UK, #ab223752; anti‐rabbit antibody).

Techniques: Expressing, Gene Expression

Journal: Cancer Medicine

Article Title: Exploring the Relationship Between Adipocytokines and Endometrial Cancer: Identifying Correlations With Clinico‐Pathological Prognostic Factors

doi: 10.1002/cam4.71007

Figure Lengend Snippet: Illustration of chromogenic 3,3′‐Diaminobenzidine (DAB) staining for ADIPOR1 (A) and ADIPOR2 (B) receptor in endometrial cancer tissue and benign tissues. Image taken at 10× magnification, Scalebar = 100 μm.

Article Snippet: The antibodies used were: ADIPOR1 (Proteintech, Manchester, UK, #66619‐1‐Ig; anti‐mouse antibody) and ADIPOR2 (Abcam, Cambridge, UK, #ab223752; anti‐rabbit antibody).

Techniques: Staining

Journal: Journal of Endocrinology

Article Title: Local adiponectin treatment reduces atherosclerotic plaque size in rabbits

doi: 10.1677/joe-06-0173

Figure Lengend Snippet: Figure 1 Serum adiponectin concentrations in rabbits. 1, Aorta non-injured rabbits on normal cholesterol diet; 2, aorta injured rabbits on high-cholesterol diet before adenovirus transfer; 3, after transfer Ad-bgal through intima; 4, after transfer Ad-APN through intima; 5, after transfer Ad-bgal through adventitia; 6, After transfer Ad-APN through adventitia. The symbol * indicates significant difference (P!0.01) vs serum adiponectin concentrations in aorta non-injured rabbits on normal cholesterol diet; † compared with rabbits before adenovirus transfer; and ‡ compared with rabbits after Ad-bgal transfer. The values are meansGS.E.M.

Article Snippet: Following blocking with 5% non-fat milk, the blots were incubated with anti-His tag mouse monoclonal antibody (1:1000 dilution, Applygen Technologies Inc., Beijing, China) and rabbit adiponectin polyclonal antibody (1:100 dilution, Boster, Wuhan, Hubei, China) at 4 8C overnight.

Techniques:

Journal: Journal of Endocrinology

Article Title: Local adiponectin treatment reduces atherosclerotic plaque size in rabbits

doi: 10.1677/joe-06-0173

Figure Lengend Snippet: Figure 2 SDS-PAGE (A) and western blot analysis of the supernatant of cultured Pichia pastoris (B, using anti-His tag monoclonal antibody; C, using adiponectin polyclonal antibody). Line 1 is a blank pGAPZa Pichia vector (not containing adiponectin gene) sample control and lines 2–4 are samples from the supernatant of cultured Pichia pastoris containing the recombinant rabbit adiponectin gene. Line 5 contained the molecular weight markers (not visible in image of blot). Both anti-His antibody and adiponectin polyclonal antibody detected a single predominant protein at z42 kDa (containing 9.3 kDa a-factor signal peptide and 2.5 kDa C-terminal His-tag).

Article Snippet: Following blocking with 5% non-fat milk, the blots were incubated with anti-His tag mouse monoclonal antibody (1:1000 dilution, Applygen Technologies Inc., Beijing, China) and rabbit adiponectin polyclonal antibody (1:100 dilution, Boster, Wuhan, Hubei, China) at 4 8C overnight.

Techniques: SDS Page, Western Blot, Cell Culture, Plasmid Preparation, Control, Recombinant, Molecular Weight

Journal: Journal of Endocrinology

Article Title: Local adiponectin treatment reduces atherosclerotic plaque size in rabbits

doi: 10.1677/joe-06-0173

Figure Lengend Snippet: Figure 5 Effect of Ad-APN treatment on adhesion molecules in atherosclerotic rabbits 14 days after recombinant adenovirus transfer. A, Immunohistochemical detection of adiponectin, VCAM-1 and ICAM-1 in atherosclerotic lesions. Representative images are shown. 1, Ad-bgal transfer through intima. 2, Ad-APN transfer through intima. 3, Ad-bgal transfer through adventitia. 4, Ad-APN transfer through adventitia. The magnification is 400. B, mRNA expression of VCAM-1 and ICAM-1 in abdominal aortic tissue in Ad-bgal or Ad-APN-treated rabbits. The values are meansGS.E.M.

Article Snippet: Following blocking with 5% non-fat milk, the blots were incubated with anti-His tag mouse monoclonal antibody (1:1000 dilution, Applygen Technologies Inc., Beijing, China) and rabbit adiponectin polyclonal antibody (1:100 dilution, Boster, Wuhan, Hubei, China) at 4 8C overnight.

Techniques: Recombinant, Immunohistochemical staining, Expressing