Journal: Nature Communications

Article Title: STAT3 is critical for skeletal development and bone homeostasis by regulating osteogenesis

doi: 10.1038/s41467-021-27273-w

Figure Lengend Snippet: a ALP and alizarin red S staining of BMSCs from Osx Cre , Osx Cre ; Stat3 fl/+ mice after culture in osteogenic medium with or without colivelin for 7 and 14 days separately. b Expression of p-STAT3 in BMSCs from Osx Cre , Osx Cre ; Stat3 fl/+ mice treated with or without colivelin for 12 h analyzed by western blotting. The experiment was performed twice to similar results. c The relative mRNA levels of Dlx5 , Runx2, Alp, Col1α1 , and Bsp in BMSCs with or without Colivelin for 14 days were quantified by qPCR. n = 9. d Micro-CT images of distal femurs from 4-week-old male Osx Cre , Osx Cre ; Stat3 fl/+ +Vehicle, and Osx Cre ; Stat3 fl/+ + Colivelin group mice. One-week-old male Osx Cre ; Stat3 fl/+ mice were used to treat with or without 1.5 mg/kg colivelin every day for 3 weeks. e Quantitative parameters of micro-CT, including BV/TV, Tb.Th., Tb.N., Tb.Sp., and Ct.Th. n = 8. f Images of calcein-alizarin red S double labeling of femurs from 4-week-old male Osx Cre , Osx Cre ; Stat3 fl/+ +Vehicle and Osx Cre ; Stat3 fl/+ + Colivelin group mice and quantitative parameters of MAR. n = 6. Two-tailed Student’s t test. Data represent the mean ± s.d. Source data are provided in the Source data file.

Article Snippet: In TS + colivelin group, mice were suspended in individual plastic cages for 7 days and with intraperitoneal administration of 1 mg/kg colivelin (MCE, HY-P1061A) for each day.

Techniques: Staining, Expressing, Western Blot, Micro-CT, Labeling, Two Tailed Test

Journal: Nature Communications

Article Title: STAT3 is critical for skeletal development and bone homeostasis by regulating osteogenesis

doi: 10.1038/s41467-021-27273-w

Figure Lengend Snippet: a Micro-CT images of distal femurs from 5-week-old Vehicle, TS + Vehicle, and TS + Colivelin group mice. Four-week-old male mice were used to perform the tail suspension model with or without 1 mg/kg colivelin every day for 1 week. b – f Quantitative parameters of micro-CT, including BV/TV, Tb.Th., Tb.N., Tb.Sp., and Ct.Th. n = 5. g Images of calcein-alizarin red S double labeling of femurs from 5-week-old Vehicle, TS + Vehicle, and TS + Colivelin group mice and quantitative parameters of MAR. n = 5. Two-tailed Student’s t test. Data represent the mean ± s.d. Source data are provided in the Source data file.

Article Snippet: In TS + colivelin group, mice were suspended in individual plastic cages for 7 days and with intraperitoneal administration of 1 mg/kg colivelin (MCE, HY-P1061A) for each day.

Techniques: Micro-CT, Suspension, Labeling, Two Tailed Test