Journal: STAR Protocols

Article Title: Murine brain tumor microenvironment immunophenotyping using mass cytometry

doi: 10.1016/j.xpro.2022.101357

Figure Lengend Snippet: Antibody master mix

Article Snippet: Ly-6C , 162Dy , HK1.4 , Fluidigm , SKU# 3162014B.

Techniques:

Journal: STAR Protocols

Article Title: Murine brain tumor microenvironment immunophenotyping using mass cytometry

doi: 10.1016/j.xpro.2022.101357

Figure Lengend Snippet:

Article Snippet: Ly-6C , 162Dy , HK1.4 , Fluidigm , SKU# 3162014B.

Techniques: Purification, Recombinant, Red Blood Cell Lysis, Centrifugation, Staining, Electron Microscopy, Antibody Labeling, Software, Sterility, Spectrophotometry

Journal: Frontiers in Cell and Developmental Biology

Article Title: Spatial immune landscapes of SARS-CoV-2 gastrointestinal infection: macrophages contribute to local tissue inflammation and gastrointestinal symptoms

doi: 10.3389/fcell.2024.1375354

Figure Lengend Snippet: Summary of antibodies and their corresponding metal tags used for imaging mass cytometry.

Article Snippet: CD8a , 162Dy , D8A8Y , Fluidigm , 1/100 , 3162035D.

Techniques: Imaging, Cytometry

Journal: Frontiers in Cell and Developmental Biology

Article Title: Spatial immune landscapes of SARS-CoV-2 gastrointestinal infection: macrophages contribute to local tissue inflammation and gastrointestinal symptoms

doi: 10.3389/fcell.2024.1375354

Figure Lengend Snippet: Gastrointestinal (GI) biopsies of COVID-19 patients showing histomorphologic changes. (A) In contrast to those of the healthy lamina propria, the gastrointestinal tracts of COVID-19 patients exhibited interstitial edema, acute inflammation (red arrow) and infiltration of plasma cells and lymphocytes (scale bar, 50 μm). (B) Immunofluorescence analysis of ACE2 and the SARS-CoV-2 nucleocapsid protein in the intestinal tissues of COVID-19 patients. ACE2 staining is shown in green, nucleocapsid staining is shown in red, and nuclear staining is shown in blue (scale bar, 50 μm). (C) Representative IMC images of gastrointestinal tissues from COVID-19 patients with (n = 12) or without (n = 4) detected NPs. Each image on the left was rendered with a selection of different markers (blue: nucleus, green: NP, red: ACE2, yellow: CD68 and CD3, magenta: CD20, cyan: E-cadherin, white: CD4 and CD8) (scale bar = 100 μm).

Article Snippet: CD8a , 162Dy , D8A8Y , Fluidigm , 1/100 , 3162035D.

Techniques: Clinical Proteomics, Immunofluorescence, Staining, Selection

Journal: Frontiers in Cell and Developmental Biology

Article Title: Spatial immune landscapes of SARS-CoV-2 gastrointestinal infection: macrophages contribute to local tissue inflammation and gastrointestinal symptoms

doi: 10.3389/fcell.2024.1375354

Figure Lengend Snippet: Macrophage recruitment in GI tissue from COVID-19 patients. (A) Cell phenotypes were visualized with a heatmap based on biomarker expression in NP + tissues (n = 12) and NP − tissues (n = 4). (B) PhenoGraph defines complex cell phenotypes based on marker expression and enables the labeling of cell phenotype clusters on a t-SNE plot. (C) Analysis by t-distributed stochastic neighbor embedding (t-SNE) dimensionality reduction yielded t-SNE plots of gastrointestinal tissues from COVID-19 patients with or without NP detection. Single-cell t-SNE maps showing the expression of CD68, CD4, CD8, and CD20. Plots showing NP − (right) in comparison to NP + (left) samples. The color spectrum on the right of the plot indicates the mean expression levels of the markers (red, high expression; blue, low expression). (D) Comparisons of the numbers of CD68 + macrophages, CD3 + CD4 + T-cells, CD3 + CD8 + T-cells, and CD20 + B-cells in the groups with or without detected NPs (Mann–Whitney test). (E) The scatter plots show a positive correlation between CD68 + macrophages and CD3 + CD4 + T-cells (n = 16), but no significant correlation was observed with CD3 + CD8 + T-cells or CD20 + B-cells (n = 16).

Article Snippet: CD8a , 162Dy , D8A8Y , Fluidigm , 1/100 , 3162035D.

Techniques: Biomarker Discovery, Expressing, Marker, Labeling, Comparison, MANN-WHITNEY

Journal: Frontiers in Cell and Developmental Biology

Article Title: Spatial immune landscapes of SARS-CoV-2 gastrointestinal infection: macrophages contribute to local tissue inflammation and gastrointestinal symptoms

doi: 10.3389/fcell.2024.1375354

Figure Lengend Snippet: Neighborhood analysis of cell–cell interactions in the GI tract of COVID-19 patients. (A, B) Unsupervised neighborhood analysis visualization of all cell-to-cell interactions based on the presence of significant adjacent (red) or avoidance (blue) interactions; white denotes the absence or nonsignificance of cell contacts (permutation test, p < 0.01). (C, D) Heatmap displaying cell–cell interactions in 4 NP − GI tract tissue samples (C) and 12 NP + tissue samples (D) from COVID-19 patients, in which the cell type in the row significantly neighbored (red) or avoided (blue) the cell type in the column. White represents a prevalence less than 10%. (E) Images from the NP − and NP + groups are displayed in the miCAT image window using user-defined color channels (blue, nucleus; green, NP; red, ACE2). Cells of interest, such as CD8 + T-cells (cluster 4, purple), CD4 + T-cells (cluster 5, gray), CD68 + macrophages (cluster 9, dark red), and CD20 + B-cells (cluster 13, turquoise), are highlighted in the image (scale bar: 100 μm).

Article Snippet: CD8a , 162Dy , D8A8Y , Fluidigm , 1/100 , 3162035D.

Techniques:

Journal: Frontiers in Physiology

Article Title: Circulating Endothelial Progenitor Cells Present an Inflammatory Phenotype and Function in Patients With Alcoholic Liver Cirrhosis

doi: 10.3389/fphys.2018.00556

Figure Lengend Snippet: Mass cytometry antibody panel information (alphabetical order).

Article Snippet: Anti-APC , APC003 , Fluidigm , Lu176.

Techniques: Mass Cytometry, Marker