Journal: bioRxiv

Article Title: Engineering chimeric DNA polymerases for DNA movable type storage

doi: 10.64898/2025.12.18.694503

Figure Lengend Snippet: Structural and biochemical characterization of 9°N DNA polymerase and chimeric variants. ( A ) Schematic domain architecture of 9°N and chimeric DNA polymerases. ( B ) Structural modeling of chimeric DNA polymerases PLS. ( C ) Thermal stability analysis of 9°N and chimeric DNA polymerases. ( D ) Salt tolerance analysis of 9°N and chimeric DNA polymerases.

Article Snippet: Deoxyribonucleoside triphosphates (dNTPs), ssM13mp18 DNA, λDNA, BsaI-HFv2, and 10× ThermoPol buffer were purchased from New England Biolabs (Ipswich, MA).

Techniques:

Journal: bioRxiv

Article Title: Engineering chimeric DNA polymerases for DNA movable type storage

doi: 10.64898/2025.12.18.694503

Figure Lengend Snippet:

Article Snippet: Deoxyribonucleoside triphosphates (dNTPs), ssM13mp18 DNA, λDNA, BsaI-HFv2, and 10× ThermoPol buffer were purchased from New England Biolabs (Ipswich, MA).

Techniques:

Journal: bioRxiv

Article Title: Engineering chimeric DNA polymerases for DNA movable type storage

doi: 10.64898/2025.12.18.694503

Figure Lengend Snippet: Interaction patterns of chimeric DNA polymerases with dsDNA. Molecular dynamics simulations of interactions between ( A ) PLS, ( B ) PLT, and ( C ) PLA DNA polymerases and dsDNA by contact fraction analysis and structural mapping of interacting residues. Hydrogen bonds were shown in yellow, π-π stacking in pink, charge-charge interactions in red, and salt bridges in purple.

Article Snippet: Deoxyribonucleoside triphosphates (dNTPs), ssM13mp18 DNA, λDNA, BsaI-HFv2, and 10× ThermoPol buffer were purchased from New England Biolabs (Ipswich, MA).

Techniques:

Journal: bioRxiv

Article Title: Engineering chimeric DNA polymerases for DNA movable type storage

doi: 10.64898/2025.12.18.694503

Figure Lengend Snippet: Information retrieval using DNA polymerases. ( A ) Schematic representation of data retrieval using orthogonal barcode. ( B ) Analysis of error types and frequencies in DNA polymerases amplification. ( C-F ) Base substitution matrices for 9°N ( C ), PLS ( D ), PLT ( E ), and PLA ( F ) DNA polymerases.

Article Snippet: Deoxyribonucleoside triphosphates (dNTPs), ssM13mp18 DNA, λDNA, BsaI-HFv2, and 10× ThermoPol buffer were purchased from New England Biolabs (Ipswich, MA).

Techniques: Amplification

Journal: bioRxiv

Article Title: Engineering chimeric DNA polymerases for DNA movable type storage

doi: 10.64898/2025.12.18.694503

Figure Lengend Snippet: Storage stability of long-term DNA movable type storage in vivo . ( A ) Bacterial passaging experiment. ( B ) Sequencing analysis of DMTB- and DMTU-engineered bacteria after serial passaging. ( C ) Sampling scheme for continuous bacterial growth. ( D ) OD600 measurement of DMTB- and DMTU-engineered bacteria under different sampling volumes.

Article Snippet: Deoxyribonucleoside triphosphates (dNTPs), ssM13mp18 DNA, λDNA, BsaI-HFv2, and 10× ThermoPol buffer were purchased from New England Biolabs (Ipswich, MA).

Techniques: In Vivo, Passaging, Sequencing, Bacteria, Sampling