Journal: Cell reports

Article Title: Caspase Activation and Caspase-Mediated Cleavage of APP Is Associated with Amyloid β-Protein-Induced Synapse Loss in Alzheimer’s Disease

doi: 10.1016/j.celrep.2020.107839

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: At 12 or 18 hours after viral infection, GSI or caspase inhibitors were added to the OTSCs: 10 μM DAPT (565770, EMD Biosciences), 10 μM zVAD (FMK001, pan-caspase inhibitor, R&D Systems), 10 μM zDEVD (FMK004, caspase-3 inhibitor, R&D Systems), 10 μM zYVAD (FMK005, caspase-4 inhibitor, R&D Systems), 10 μM zVEID (FMK006, caspase-6 inhibitor, R&D Systems), and 10 μM zIETD (FMK007, caspase-8 inhibitor, R&D Systems).

Techniques: Virus, Recombinant, Plasmid Preparation, Protease Inhibitor, Transfection, Software, Cell Culture

Journal: Neuroscience Bulletin

Article Title: Sex-Dependent Glial Signaling in Pathological Pain: Distinct Roles of Spinal Microglia and Astrocytes

doi: 10.1007/s12264-017-0145-y

Figure Lengend Snippet: Effects of spinal injection of CASP6 and TNF on naïve male and female mice. A Intrathecal injection of CASP6 (5 U) and TNF (20 ng) both elicited rapid and persistent mechanical hypersensitivity in naïve males. B Intrathecal injection of TNF but not CASP6 elicited rapid and persistent mechanical hypersensitivity in naïve females. Mean ± SEM; *P < 0.05, n.s., not significant, two-way ANOVA; n = 4 mice per group; BL, baseline. Arrows indicate drug injection.

Article Snippet: Recombinant full-length active human caspase-6 protein (CASP6) was from Abcam (Cambridge, MA), the CASP6 inhibitor Z-V-E(OMe)-I-D(OMe)-FMK (ZVEID) and TNF were from R&D Systems (Minneapolis, MN).

Techniques: Injection

Journal: Neuroscience Bulletin

Article Title: Sex-Dependent Glial Signaling in Pathological Pain: Distinct Roles of Spinal Microglia and Astrocytes

doi: 10.1007/s12264-017-0145-y

Figure Lengend Snippet: CASP6 contributes to formalin-induced pain and late-phase mechanical hypersensitivity in male mice. A Time course of formalin-induced pain in males and females with intrathecal vehicle or the CASP6 inhibitor ZVEID (30 µg). B ZVEID (30 µg, i.t.) reduced both formalin-induced phase I and phase II pain in males but only phase I in females. *P < 0.05, two-tailed Student’s t-test, n = 5 mice per group. C, D Formalin-induced pain was reduced in male but not female Casp6 –/– mice. Mean ± SEM; *P < 0.05, two-tailed Student’s t-test, n = 4–5 mice per group. E Formalin-induced mechanical hypersensitivity in the late phase was lower in male than in female Casp6 –/– mice. Mean ± SEM; *P < 0.05, two-way ANOVA; n = 4–5 mice per group.

Article Snippet: Recombinant full-length active human caspase-6 protein (CASP6) was from Abcam (Cambridge, MA), the CASP6 inhibitor Z-V-E(OMe)-I-D(OMe)-FMK (ZVEID) and TNF were from R&D Systems (Minneapolis, MN).

Techniques: Two Tailed Test