Journal: BMC gastroenterology

Article Title: ZBTB10 as a potential prognosis biomarker and correlates with the tumor immune microenvironment in stomach adenocarcinoma.

doi: 10.1186/s12876-025-04047-y

Figure Lengend Snippet: Fig. 3 The clinical characteristics of ZBTB10 expression in STAD tissues. Box-plot showing the ZBTB10 mRNA median expression level correlated with tumor grade (A), pathological stages (B), lymph node metastases (C), patient’s ages(D), TP53 mutation status (E), and H.pylori infection status (F) respectively. The median expression of ZBTB10 was analyzed using the Mann–Whitney U test for two-group comparisons and the Kruskal–Wallis (K-W) test for multiple independent groups respectively. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: The membranes were then blocked with 5% non-fat milk at room temperature for 1 h, followed by incubation with primary antibodies against ZBTB10 (1:1000, Proteintech, China) and GAPDH (1:1000, Abcam) overnight at 4 °C.

Techniques: Expressing, Mutagenesis, Infection, MANN-WHITNEY

Journal: BMC gastroenterology

Article Title: ZBTB10 as a potential prognosis biomarker and correlates with the tumor immune microenvironment in stomach adenocarcinoma.

doi: 10.1186/s12876-025-04047-y

Figure Lengend Snippet: Fig. 6 Correlation of ZBTB10 expression with immune characteristics. (A) Radar map displayed the association between ZBTB10 median expression and tumor mutational burden (TMB). (B) The radar map displayed the association between ZBTB10 median expression and microsatellite instability (MSI). (C) Comparison of immune score and stromal score based on ZBTB10 median expression. (D) 22 types of tumor-infiltrating immune cells were analyzed based on ZBTB10 median expression using the CIBERSORT algorithm. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: The membranes were then blocked with 5% non-fat milk at room temperature for 1 h, followed by incubation with primary antibodies against ZBTB10 (1:1000, Proteintech, China) and GAPDH (1:1000, Abcam) overnight at 4 °C.

Techniques: Expressing, Comparison

Journal: BMC gastroenterology

Article Title: ZBTB10 as a potential prognosis biomarker and correlates with the tumor immune microenvironment in stomach adenocarcinoma.

doi: 10.1186/s12876-025-04047-y

Figure Lengend Snippet: Fig. 7 The association of ZBTB10 expression with tumor immune microenvironment. (A) ZBTB10 median expression correlated with tumor-infiltrating lymphocytes. (B) ZBTB10 median expression correlated with immune stimulators. (C) ZBTB10 median expression correlated with immune inhibitors. (D) The correlation analysis of the top four targeting immunosuppressive and immuneactivative markers with ZBTB10 median expression in STAD. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: The membranes were then blocked with 5% non-fat milk at room temperature for 1 h, followed by incubation with primary antibodies against ZBTB10 (1:1000, Proteintech, China) and GAPDH (1:1000, Abcam) overnight at 4 °C.

Techniques: Expressing

Journal: BMC gastroenterology

Article Title: ZBTB10 as a potential prognosis biomarker and correlates with the tumor immune microenvironment in stomach adenocarcinoma.

doi: 10.1186/s12876-025-04047-y

Figure Lengend Snippet: Fig. 10 Multiplex immunohistochemistry (mIHC) staining for identification. Representative mIHC images of STAD tissues and adjacent normal tissues, a staining panel was developed to visualize CD45, EpCAM, ZBTB10 and DAPI simultaneously. CD45: leukocyte marker. EpCAM: epithelial cell marker. Scale bar 200 µm

Article Snippet: The membranes were then blocked with 5% non-fat milk at room temperature for 1 h, followed by incubation with primary antibodies against ZBTB10 (1:1000, Proteintech, China) and GAPDH (1:1000, Abcam) overnight at 4 °C.

Techniques: Multiplex Assay, Immunohistochemistry, Staining, Marker