ythdc2 (Cell Signaling Technology Inc)
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ythdc2
Ythdc2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ythdc2/YTHDF3+Antibody/bio_rxiv__64898__2026__03__09__710444-135-19-20
Average 94 stars, based on 31 article reviews
Ythdc2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ythdc2/YTHDF3+Antibody/bio_rxiv__64898__2026__03__09__710444-135-19-20
Average 94 stars, based on 31 article reviews
ythdc2 - by Bioz Stars,
2026-09
94/100 stars
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other:Article Title: Inhibition of BRD proteins suppresses the phenotype of uterine fibroids Article Snippet: Specific protein bands were visualized using ChemiDoc XRS molecular imager (Bio-Rad, Hercules, CA, USA). SDS Page:Article Title: METTL3-mediated m6A modification of TACSTD2 mRNA inhibits papillary thyroid cancer progression by activating the TNF signaling and blocking epithelial-mesenchymal transition. Article Snippet: Utilizing RIPA lysis buffer enriched with protease inhibitors, total protein was extracted from cells and then assessed by a BCA Protein Assay Kit (Beyotime Biotechnology, China). .. Equal protein amounts were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, Germany), blocked with 5% non-fat milk at room temperature for 2 h prior to incubation overnight at 4°C with primary antibodies against the following proteins: TACSTD2 (CST, #76730), METTL3 (CST, #86132), Article Title: METTL3-mediated m6A modification of TACSTD2 mRNA inhibits papillary thyroid cancer progression by activating the TNF signaling and blocking epithelial–mesenchymal transition Article Snippet: Utilizing RIPA lysis buffer enriched with protease inhibitors, total protein was extracted from cells and then assessed by a BCA Protein Assay Kit (Beyotime Biotechnology, China). .. Equal protein amounts were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, Germany), blocked with 5% non-fat milk at room temperature for 2 h prior to incubation overnight at 4 °C with primary antibodies against the following proteins: TACSTD2 (CST, #76730), METTL3 (CST, #86132), Incubation:Article Title: METTL3-mediated m6A modification of TACSTD2 mRNA inhibits papillary thyroid cancer progression by activating the TNF signaling and blocking epithelial-mesenchymal transition. Article Snippet: Utilizing RIPA lysis buffer enriched with protease inhibitors, total protein was extracted from cells and then assessed by a BCA Protein Assay Kit (Beyotime Biotechnology, China). .. Equal protein amounts were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, Germany), blocked with 5% non-fat milk at room temperature for 2 h prior to incubation overnight at 4°C with primary antibodies against the following proteins: TACSTD2 (CST, #76730), METTL3 (CST, #86132), Article Title: METTL3-mediated m6A modification of TACSTD2 mRNA inhibits papillary thyroid cancer progression by activating the TNF signaling and blocking epithelial–mesenchymal transition Article Snippet: Utilizing RIPA lysis buffer enriched with protease inhibitors, total protein was extracted from cells and then assessed by a BCA Protein Assay Kit (Beyotime Biotechnology, China). .. Equal protein amounts were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, Germany), blocked with 5% non-fat milk at room temperature for 2 h prior to incubation overnight at 4 °C with primary antibodies against the following proteins: TACSTD2 (CST, #76730), METTL3 (CST, #86132), Article Title: N6-methyladenosine RNA landscape in the aged mouse hearts Article Snippet: .. Primary antibody incubation occurred at 4 °C overnight and secondary incubation occurred at room temperature for 1 h. Primary antibodies used were: anti p16 (1:500, Santa), anti p53 (1:1,000, CST), anti γH2A.X (1:1,000, Santa), anti METTL3 (1:1,000, Abcam), anti METTL14 (1:1,000, CST), anti WTAP (1:1,000, Proteintech), anti FTO (1:1,000, CST), anti ALKBH5 (1:1,000, Proteintech), anti YTHDC1 (1:1,000, CST), anti Article Title: Regulation and Function of the HPV16 CircE7 RNA Article Snippet: For western blot analysis, cells were lysed in Cell Lysis Buffer (Cell Signaling Technologies, 5872S), separated on SDS-PAGE gels, and transferred to PVDF membranes. .. Membranes were incubated overnight at 4°C with the following primary antibodies: YTHDC1 (Cell Signaling, 77422), YTHDF3 (Cell Signaling, 24206), Blocking Assay:Article Title: FTO deficiency in older livers exacerbates ferroptosis during ischaemia/reperfusion injury by upregulating ACSL4 and TFRC Article Snippet: Then, the proteins in each group were separated by 12% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and transferred to polyvinylidene difluoride membranes (PVDF, 0.45 μm, Merck Millipore, Billerica, MA, USA). .. Subsequently, 5% nonfat milk was used to block the nonspecific antigens in the membranes for 1 h followed by incubating the membranes with the primary antibodies [including antibodies against NRF2 (1:500; Cell Signaling Technology, Danvers, MA, USA), FTH1 (1:2000; Cell Signaling Technology, Danvers, MA, USA), CISD1/mitoNEET (1:2000; Cell Signaling Technology, Danvers, MA, USA), FTO (1:1000; Cell Signaling Technology, Danvers, MA, USA), Rb (1:1000; Cell Signaling Technology, Danvers, MA, USA), Phospho-Rb (Ser807/811) (1:1000; Cell Signaling Technology, Danvers, MA, USA), Phospho-Rb (Ser780) (1:1000; Cell Signaling Technology, Danvers, MA, USA), β-Actin (1:2000; Cell Signaling Technology, Danvers, MA, USA), p53 (1:1000; Abcam, Cambridge, MA), Glutathione Peroxidase 4 (1:5000; Abcam, Cambridge, MA), p27 Kip1 (1:500; Santa Cruz, USA), p21 Waf1/Cip1 (1:500; Santa Cruz, USA), Flag (1:1000; Cell Signaling Technology, Danvers, MA, USA) YTHDF2 (1:500; Cell Signaling Technology, Danvers, MA, USA) and Wilms Tumor Assay:Article Title: Identification of differential <scp>m6A RNA</scp> methylomes and <scp>ALKBH5</scp> as a potential prevention target in the developmental neurotoxicity induced by multiple sevoflurane exposures Article Snippet: © 2024 Federation of American Societies for Experimental Biology.. Xiaowen Meng, Yichan Wang and Weiming Zhao contributed equally to this work.. Abbreviations: 3′UTRs, 3′ untranslated regions; 5′UTRs, 5′ untranslated regions; ALKBH5, alkB homolog 5; AMPK, adenosine 5′monophosphate (AMP)activated protein kinase; Aqp4, aquaporin4; Atg4d, autophagyrelated 4D cysteine peptidase; B3galt2, β1,3Galactosyltransferase 2; BCA, bicinchoninic acid; Bod1l, biorientation of chromosomes in cell division protein 1like; CBLL1, Casitas Blineage protooncogene like 1; CDSs, coding sequences; Chst1, carbohydrate sulfotransferase 1; DEGs, differentially expressed genes; DMGs, differentially methylated m6A genes; Dnajb4, DnaJ heatshockprotein family (Hsp40) member B4; Dnmbp, dynaminbinding protein; ECL, electrochemiluminescence; ELAVL1, embryonic lethalabnormal vision like protein 1; EPM, elevated plus maze; FMR1, fragile X mentalretardation 1; FTO, fat mass and obesityassociated protein; Gigyf1, GRB10 interacting GYF protein 1; GO, gene ontology; Gpr22, Gprotein coupled receptor 22; HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2B1; HNRNPC, heterogeneous nuclear ribonucleoprotein C; HRP, horseradish peroxidase; Hspa5, heatshock protein family A member 5; IGF2BP1, insulinlike growth factor2 mRNAbinding protein 1; IP, immunoprecipitation; Jph1, junctophilin 1; KEGG, Kyoto Encyclopedia of Genes and Genomes; LRPPRC, leucinerich pentatricopeptide repeat containing; m6A, N6Methyladenosine; m6Aseq, m6Asequencing; Manf, mesencephalic astrocytederived neurotrophic factor; MBP, myelin basic protein; METTL14, methyltransferaselike 14; METTL3, methyltransferaselike 3; mRNAs, messenger RNAs; MWM, Morris water maze; NOR, novel object recognition; O.C.T., optimal cutting temperature; Pcdhgb8, protocadherin gamma subfamily B8; Phf3, PHD finger protein 3; PND, postnatal days; PPI, protein–protein interaction; PVDF, polyvinylidene fluoride; Rbm12b2, RNAbinding protein 12BB; RBM15, RNAbinding motif protein 15; RBM15B, RNAbinding motif protein 15B; RIN, RNA integrity number; RNAseq, RNAsequencing; SDSPAGE, sodium dodecyl sulfatepolyacrylamide gel electrophoresis; Slc25a37, solute carrier family 25 member 37; Spef1, sperm flagellar protein 1; Traf6, tumor necrosis factor receptorassociated factor 6; TSS, transcription starting site; WTAP, Wilms tumor 1associated protein; YTHDC, YTH domain containing; YTHDF, YTH domain family; ZC3H13, zinc finger CCCHtype containing 13. |
