Journal: Molecular & Cellular Oncology

Article Title: GINS1 suppresses ferroptosis by inhibiting the Hippo pathway in neuroblastoma

doi: 10.1080/23723556.2026.2715868

Figure Lengend Snippet: The impact of GINS1 on ferroptosis and the Hippo signaling pathway in neuroblastoma cells. A GO and KEGG pathway enrichment analysis of GINS1. B The ssGSEA scores of the ferroptosis pathway in Low_GINS1 and High_GINS1 groups. C The correlation between GINS1 expression and the expression of ACSL4, AIFM2, and TP53 proteins. D The gene expression in ferroptosis and Hippo pathways between neuroblastoma in Low_GINS1 and High_GINS1 groups; red boxes highlight ferroptosis genes. E The relative RFU of C11-BODIPY 581/591 were measured to assess lipid peroxidation in SH-SY5Y neuroblastoma cells transfected with shNC, shGINS1-1 and shGINS1-2. The concentration of malondialdehyde (MDA) and the iron content were also determined, respectively, as indicators of lipid peroxidation and iron metabolism. F Western blot assessed ACSL4, YAP, p -YAP, and AIFM2 in SH-SY5Y cells with shNC, shGINS1-1, and shGINS1-2. Data are presented as mean ± SD from three independent experiments, and statistical significance was determined using unpaired t-test or one-way ANOVA. The p -values are denoted by asterisks as follows: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant.

Article Snippet: Membranes were incubated with primary antibodies against ACSL4 (1:2000, Proteintech, 22401-1-AP), p -YAP (1:1000, Proteintech, 80694-2-RR), YAP (1:1000, Proteintech, 13584-1-AP), AIFM2 (1:1000, Proteintech, 20886-1-AP), p -MST1 (1:1000, Cell Signaling Technology, #3681S), MST1 (1:1000, Cell Signaling Technology, #3682S), p -LATS1 (1:1000, Cell Signaling Technology, #8654S), LATS1 (1:1000, Cell Signaling Technology, #3477S), GPX4 (1:1000, Proteintech, 67763-1-Ig), SLC7A11 (1:1000, Proteintech, 26864-1-AP) and GAPDH (1:30000, Proteintech, 60004-1-lg), followed by HRP-conjugated secondary antibodies.

Techniques: Analysis, Expressing, Gene Expression, Transfection, Concentration Assay, Western Blot

Journal: Advanced Science

Article Title: Arrayed Magnetic Accelerator Synergistically Enhances Orthodontic Tooth Movement via Microtubule Assembly‐Mediated Periodontal Remodeling

doi: 10.1002/advs.77447

Figure Lengend Snippet: SMFs amplify the response of orthodontic mechanical signaling pathways. (a) Representative immunofluorescence images of vinculin (green), F‐actin (purple), and nuclei (blue) in PDLCs under three conditions: control (without mechanical force and without SMFs), with mechanical force only, and with mechanical force plus SMFs. Scale bar: 10 µm. (b) Cell area, (c) FA area per cell, and (d) cell shape index in PDLCs. Mean ± SD. n = 24. * p < 0.05, ** p < 0.01, **** p < 0.0001. (e) FAK: Focal Adhesion Kinase. It is a mechanotransducer that becomes activated upon orthodontic force, regulating downstream bone remodeling and inflammatory responses in PDLCs. Representative immunofluorescence images of FAK (red) and nuclei (blue) in PDLCs under three conditions: control (without mechanical force and without SMFs), mechanical force only, and mechanical force plus SMFs. Scale bar: 10 µm. (f) Relative FAK integrated density per cell in PDLCs. Mean ± SD. n = 24. **** p < 0.0001. (g) Representative immunofluorescence images of YAP/TAZ (green) and nuclei (blue) in PDLCs under three conditions: control (without mechanical force and without SMFs), mechanical force only, and mechanical force plus SMFs. Scale bar: 10 µm. (h) The nuclear‐cytoplasmic ratios of YAP/TAZ in PDLCs analyzed from the immunofluorescence images. Mean ± SD. n = 24. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: PDLCs were respectively incubated with the primary antibody for Vinculin (Proteintech, 26520‐1‐Ap, 1:300), FAK (Proteintech, 12636‐1‐AP, 1:400), YAP/TAZ (Proteintech, 13584‐1‐Ap, 1:200), α‐Tubulin (Abclonal, AC012, 1:200) and Integrin‐β1 (Abclonal, A23497, 1:200).

Techniques: Protein-Protein interactions, Immunofluorescence, Control

Journal: International Journal of Biological Sciences

Article Title: Young plasma-derived exosomes deliver YOD1 to induce M2 polarization and ameliorate osteoporosis via mTOR signaling pathways

doi: 10.7150/ijbs.138620

Figure Lengend Snippet: Mechanistic analysis of YOD1-mediated regulation by Y-Exos in macrophage polarization and osteogenic differentiation. (A) PCA analysis of transcriptomic profiles of LPS-stimulated BMMs treated with or without YOD1. (B) Number of differentially expressed genes identified after YOD1 treatment (P < 0.05, |log₂(FC)| > 1). (C) KEGG pathway enrichment analysis of differentially expressed genes. (D) Western blot analysis of Hippo/YAP signaling-related proteins and macrophage polarization markers in BMMs treated with YOD1 or Y-Exos under LPS stimulation. (E) Effects of YAP1 nuclear translocation inhibition by Verteporfin on ARG1 and iNOS expression in BMMs. (F-G) Quantification of protein expression in (D-E). (H) Western blot analysis of AKT/mTOR signaling-related proteins in BMMs after YOD1 or Y-Exos treatment. (I) Quantification of AKT/mTOR-related protein expression. (J) Effects of AKT and mTOR inhibition by MK2206 and Rapamycin on macrophage polarization-related proteins. (K) Western blot analysis of Hippo/YAP signaling-related proteins and osteogenic markers in BMSCs after YOD1 or Y-Exos treatment during osteogenic induction. (L-M) Quantification of protein expression in (J-K). (N) F-actin ring staining showing the effects of Rapamycin on the indirect regulation of osteoclast differentiation by YOD1 and Y-Exos (scale bar = 100 μm). (O-P) Western blot analysis and quantification of NFATC1 expression after Rapamycin treatment. Data are presented as mean ± SD. *P < 0.05; NS, no significant difference.

Article Snippet: The primary and secondary antibodies are listed as follows: TSG101 Rabbit antibody (Proteintech, China, 28283-1-AP), CD63 Rabbit antibody (Proteintech, China, 25682-1-AP), CD9 Rabbit antibody (Proteintech, China, 20597-1-AP), Calnexin Rabbit antibody (Proteintech, China, 10427-2-AP), CD86 Rabbit antibody(Immunoway, USA, YM8023), CD206 Rabbit antibody (Immunoway, USA, YM8349), iNOS Rabbit antibody(Proteintech, China, 22226-1-AP), ARG1 Rabbit antibody (Abcam, China, ab96183), RUNX2 Rabbit antibody (Proteintech, China, 20700-1-AP), Osteocalcin Rabbit antibody(Servicebio, China, GB11233-100), ACTIN Mouse antibody(Proteintech, China, 66009-1-Ig), YOD1 Rabbit antibody (Proteintech, China, 25370-1-AP), IDE Rabbit antibody (Proteintech, China, 21728-1-AP), VCL Rabbit antibody (Proteintech, China, 26520-1-AP), GAPDH Rabbit antibody (BOSTER, China, A00227-1), ITCH Rabbit antibody (Proteintech, China, 20920-1-AP), LATS1 Rabbit antibody (Proteintech, China, 17049-1-AP), Phospho-YAP1 (Ser127) Rabbit antibody (Proteintech, China, 80694-2-RR), YAP1 Rabbit antibody (Proteintech, China, 13584-1-AP), Phospho-AKT Rabbit antibody (Proteintech, China, 29163-1-AP), AKT Rabbit antibody (Proteintech, China, 10176-2-AP), Phospho-mTOR Rabbit antibody(ZenBio, China, 381557), mTOR Rabbit antibody (HUA BIO, China, ET1608-5), NFATC1 Mouse antibody (Proteintech, China, 66963-1-Ig).

Techniques: Analysis, Western Blot, Translocation Assay, Inhibition, Expressing, Staining, Cell Differentiation